Restriction map for
pFN21AB7637
(Circular) (Six-base) MAPSORT of: pFN21AB7637.seq Check: 5765 from: 1 to: 5683
pFN21AB7637 5683 bp
With 182 enzymes: SgfI *
March 28, 2012 12:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 2578 3810 4183 1563
Size: 1015 1232 373 3063
Fragments arranged by size:
3063 1232 1015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 2116 4412 2116
Size: 2296 3387
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2164 2168 1184
Size: 980 4 4699
Fragments arranged by size:
4699 980 4
AlwNI CAG_nnn'CTG
Cuts at: 3426 4998 3426
Size: 1572 4111
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 1870
Size: 73 1065 4545
Fragments arranged by size:
4545 1065 73
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2406 3004 4459 618
Size: 325 217 627 619 598 1455 1842
Fragments arranged by size:
1842 1455 627 619 598 325 217
BanII G_rGCy'C
Cuts at: 1391 2042 2517 3002 1391
Size: 651 475 485 4072
Fragments arranged by size:
4072 651 485 475
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2910 928
Size: 971 1011 3701
Fragments arranged by size:
3701 1011 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2009 2043 3874 3908 1903
Size: 34 72 34 1831 34 3678
Fragments arranged by size:
3678 1831 72 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2395 3575 4785 2395
Size: 1180 1210 3293
Fragments arranged by size:
3293 1210 1180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BfrBI ATG'CAT
Cuts at: 2721 2885 2721
Size: 164 5519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2832 3744 4900 1790
Size: 1042 912 1156 2573
Fragments arranged by size:
2573 1156 1042 912
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 3414 4372
Size: 416 53 83 399 24 1457 540 958
Cuts at: 4372 442
Size: 1753
Fragments arranged by size:
1753 1457 958 540 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2465 4343 1239
Size: 869 357 1878 2579
Fragments arranged by size:
2579 1878 869 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 3808 4673 4971 2018
Size: 1790 865 298 2730
Fragments arranged by size:
2730 1790 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2191 2732 2827 3474 493
Size: 711 987 541 95 647 2702
Fragments arranged by size:
2702 987 711 647 541 95
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2726 2756 1178
Size: 30 165 30 1323 30 4105
Fragments arranged by size:
4105 1323 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 1186
Size: 1267 4416
BseYI C'CCAG_C
Cuts at: 4886 5382 4886
Size: 496 5187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 1163
Size: 573 438 4672
Fragments arranged by size:
4672 573 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 2517 2958 3744 3829 4900 2094
Size: 423 441 786 85 1071 2877
Fragments arranged by size:
2877 1071 786 441 423 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
BsmBI CGTCTCn'nnnn_
Cuts at: 2096 2096
Size: 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2094 2517 2832 2958 3002 3744
Size: 399 252 52 423 315 126 44 742
Cuts at: 3744 3829 4900 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 399 315 252 126
85 52 44
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 844
Size: 2194 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2386 4178 4352 65
Size: 1183 1138 1792 174 1396
Fragments arranged by size:
1792 1396 1183 1138 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2191 2690 794
Size: 1397 499 3787
Fragments arranged by size:
3787 1397 499
BstBI TT'CG_AA
Cuts at: 2938 4487 2938
Size: 1549 4134
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678
Size: 1041 367 385 17 1452 86 12 346
Cuts at: 5678 1972
Size: 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2979 513
Size: 552 939 975 3217
Fragments arranged by size:
3217 975 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2584 3130 4019
Size: 54 1425 50 275 18 754 546 889
Cuts at: 4019 5648 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 754 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2862 3613 1353
Size: 1509 751 3423
Fragments arranged by size:
3423 1509 751
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2515 2515
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2465 3746 4343 5130 5398 1239
Size: 393 476 357 1281 597 787 268 1524
Fragments arranged by size:
1524 1281 787 597 476 393 357 268
EcoO109I rG'GnC_Cy
Cuts at: 2081 2205 2081
Size: 124 5559
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2934 2934
Size: 5683
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2726 2758 4324
Size: 32 135 32 530 32 592 32 1566
Cuts at: 4324 4356 4398 4430 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 592 530 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2586 10
Size: 54 1425 343 754 3107
Fragments arranged by size:
3107 1425 754 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3099 3776 4924 5169 1606
Size: 1493 677 1148 245 2120
Fragments arranged by size:
2120 1493 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2979 513
Size: 552 939 975 3217
Fragments arranged by size:
3217 975 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NsiI A_TGCA'T
Cuts at: 2723 2887 2723
Size: 164 5519
NspI r_CATG'y
Cuts at: 1132 1229 2725 2885 3041 4586 1132
Size: 97 1496 160 156 1545 2229
Fragments arranged by size:
2229 1545 1496 160 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PmlI CAC'GTG
Cuts at: 2191 2732 2827 2191
Size: 541 95 5047
Fragments arranged by size:
5047 541 95
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2205 2081
Size: 124 5559
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PstI C_TGCA'G
Cuts at: 830 2143 3035 830
Size: 1313 892 3478
Fragments arranged by size:
3478 1313 892
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
SacI G_AGCT'C
Cuts at: 2517 2517
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2139 3031 4166 4847 5038 826
Size: 219 1094 892 1135 681 191 1471
Fragments arranged by size:
1471 1135 1094 892 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 3787 4688 4950 820
Size: 197 926 90 1754 901 262 1553
Fragments arranged by size:
1754 1553 926 901 262 197 90
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 2968 3607 5
Size: 47 2916 639 2081
Fragments arranged by size:
2916 2081 639 47
StuI AGG'CCT
Cuts at: 1517 2159 1517
Size: 642 5041
StyI C'CwwG_G
Cuts at: 513 1065 2004 2642 2949 2979 3122 513
Size: 552 939 638 307 30 143 3074
Fragments arranged by size:
3074 939 638 552 307 143 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3710 3727 3880 4065 4510 325
Size: 1255 2130 17 153 185 445 1498
Fragments arranged by size:
2130 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 2816 3812 2816
Size: 996 4687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI