Restriction map for
pFN21AB7634
(Circular) (Six-base) MAPSORT of: pFN21AB7634.seq Check: 1125 from: 1 to: 5830
pFN21AB7634 5830 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3067 3957 4330 1563
Size: 1504 890 373 3063
Fragments arranged by size:
3063 1504 890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2280 2388 2675 3893 5277 5545 1632
Size: 648 108 287 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 648 287 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 2536 4559 2536
Size: 2023 3807
AleI CACnn'nnGTG
Cuts at: 1166 2484 1166
Size: 1318 4512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2374 2461 2877 3139 3365 3517 5626 1493
Size: 881 87 416 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 416 262 226 152 87
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 2724 3160 3527 2724
Size: 436 367 5027
Fragments arranged by size:
5027 436 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3056 3151 4606 618
Size: 325 217 627 1269 95 1455 1842
Fragments arranged by size:
1842 1455 1269 627 325 217 95
BanII G_rGCy'C
Cuts at: 1391 2130 2736 3149 1391
Size: 739 606 413 4072
Fragments arranged by size:
4072 739 606 413
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2232 2266 4021 4055 1903
Size: 34 295 34 1755 34 3678
Fragments arranged by size:
3678 1755 295 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2230 3722 4932 2230
Size: 1492 1210 3128
Fragments arranged by size:
3128 1492 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BfrBI ATG'CAT
Cuts at: 2225 2225
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3075 5825 3075
Size: 2750 3080
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 3891 5047 1790
Size: 2101 1156 2573
Fragments arranged by size:
2573 2101 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2186 3561 4519
Size: 416 53 83 399 24 769 1375 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1375 958 769 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 4490 1239
Size: 905 2346 2579
Fragments arranged by size:
2579 2346 905
Bpu10I CC'TnA_GC
Cuts at: 2151 2151
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 2181 4493 882
Size: 617 609 73 2312 2219
Fragments arranged by size:
2312 2219 617 609 73
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2233 4263 4661 4935 5082 5493 2233
Size: 2030 398 274 147 411 2570
Fragments arranged by size:
2570 2030 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 1178
Size: 30 165 30 1113 30 4462
Fragments arranged by size:
4462 1113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 1186
Size: 940 4890
BseYI C'CCAG_C
Cuts at: 2414 2434 5033 5529 2414
Size: 20 2599 496 2715
Fragments arranged by size:
2715 2599 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 2761 2858 1163
Size: 573 726 299 97 4135
Fragments arranged by size:
4135 726 573 299 97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 3891 3976 5047 2130
Size: 1761 85 1071 2913
Fragments arranged by size:
2913 1761 1071 85
BsmI GAATG_Cn'
Cuts at: 2227 3341 3434 2227
Size: 1114 93 4623
Fragments arranged by size:
4623 1114 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2736 3149 3891 3976 5047 1391
Size: 399 340 606 413 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 413 399 340 85
BspEI T'CCGG_A
Cuts at: 2233 5493 2233
Size: 3260 2570
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2150 3717 1394
Size: 429 206 121 1567 3507
Fragments arranged by size:
3507 1567 429 206 121
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 2211 96
Size: 2115 3715
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 2829 3890 4902 2829
Size: 1061 1012 3757
Fragments arranged by size:
3757 1061 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2681 794
Size: 1887 3943
BstBI TT'CG_AA
Cuts at: 2252 4634 2252
Size: 2382 3448
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstXI CCAn_nnnn'nTGG
Cuts at: 2923 2923
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2296 2650 2724 3075 3160 3527 3912 3929
Size: 324 354 74 351 85 367 385 17
Cuts at: 3929 5381 5467 5479 5825 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 354 351 346 324
86 85 74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 513
Size: 552 939 96 4243
Fragments arranged by size:
4243 939 552 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 2875 3121 3134 2875
Size: 246 13 5571
Fragments arranged by size:
5571 246 13
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2345 4837 809
Size: 507 1029 2492 1802
Fragments arranged by size:
2492 1802 1029 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 3277 4166
Size: 54 1425 50 275 18 191 1256 889
Cuts at: 4166 5795 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1256 889 275 191 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2790 3760 1353
Size: 1437 970 3423
Fragments arranged by size:
3423 1437 970
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 5830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2280 2388 2675 3893 4490 5277
Size: 393 512 136 108 287 1218 597 787
Cuts at: 5277 5545 1239
Size: 268 1524
Fragments arranged by size:
1524 1218 787 597 512 393 287 268
136 108
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2480 2512 2522
Size: 32 135 32 701 32 175 32 10
Cuts at: 2522 2554 2901 2933 4471 4503 4545 4577 1373
Size: 32 347 32 1538 32 42 32 2626
Fragments arranged by size:
2626 1538 701 347 175 135 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3174 3420 669
Size: 988 281 1113 123 246 3079
Fragments arranged by size:
3079 1113 988 281 246 123
HindIII A'AGCT_T
Cuts at: 748 2843 748
Size: 2095 3735
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 2628 2872 4944 5128 1305
Size: 1248 75 244 2072 184 2007
Fragments arranged by size:
2072 2007 1248 244 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2484 3788 4147 4306 518
Size: 648 1318 1304 359 159 2042
Fragments arranged by size:
2042 1318 1304 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2476 3246 3923 5071 5316 1606
Size: 870 770 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 770 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2100 513
Size: 552 939 96 4243
Fragments arranged by size:
4243 939 552 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NsiI A_TGCA'T
Cuts at: 2227 2227
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2675 3188 4733 1132
Size: 97 1446 513 1545 2229
Fragments arranged by size:
2229 1545 1446 513 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2476 2476
Size: 5830
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2790 2790
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2609 3178 4313 4994 5185 826
Size: 219 1564 569 1135 681 191 1471
Fragments arranged by size:
1564 1471 1135 681 569 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 2675 3188 1132
Size: 1543 513 3774
Fragments arranged by size:
3774 1543 513
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2004 2100 3269 513
Size: 552 939 96 1169 3074
Fragments arranged by size:
3074 1169 939 552 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2185 2428 3857 3874 4027 4212 4657
Size: 1255 605 243 1429 17 153 185 445
Cuts at: 4657 325
Size: 1498
Fragments arranged by size:
1498 1429 1255 605 445 243 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 4076 5788
Size: 274 80 33 51 1443 234 1865 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1865 1712 1443 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3030 3062 5790 5822 3030
Size: 32 2728 32 3038
Fragments arranged by size:
3038 2728 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2498 1316
Size: 1182 4648
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2632 2632
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 2976 3959 2976
Size: 983 4847
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI