Restriction map for
pFN21AB7633
(Circular) (Six-base) MAPSORT of: pFN21AB7633.seq Check: 9588 from: 1 to: 5668
pFN21AB7633 5668 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2573 2722 3731 5115 5383 1632
Size: 605 336 149 1009 1384 268 1917
Fragments arranged by size:
1917 1384 1009 605 336 268 149
AflII C'TTAA_G
Cuts at: 820 1017 2420 2672 820
Size: 197 1403 252 3816
Fragments arranged by size:
3816 1403 252 197
AflIII A'CryG_T
Cuts at: 1203 2596 2848 4567 5544 1203
Size: 1393 252 1719 977 1327
Fragments arranged by size:
1719 1393 1327 977 252
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 2781 1166
Size: 1615 4053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2388 2640 3411 4983 2388
Size: 252 771 1572 3073
Fragments arranged by size:
3073 1572 771 252
ApaLI G'TGCA_C
Cuts at: 2510 2762 3725 4881 2510
Size: 252 963 1156 3297
Fragments arranged by size:
3297 1156 963 252
ApoI r'AATT_y
Cuts at: 1493 2235 2827 2977 3203 3355 5464 1493
Size: 742 592 150 226 152 2109 1697
Fragments arranged by size:
2109 1697 742 592 226 152 150
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2989 4444 618
Size: 325 217 627 1202 1455 1842
Fragments arranged by size:
1842 1455 1202 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2498 2987 1391
Size: 1107 489 4072
Fragments arranged by size:
4072 1107 489
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 2406 928
Size: 971 326 181 4190
Fragments arranged by size:
4190 971 326 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 2467 1548
Size: 919 4749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2435 5663 2435
Size: 3228 2440
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2514 2766 3729 4885 1790
Size: 724 252 963 1156 2573
Fragments arranged by size:
2573 1156 963 724 252
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 3399 4357
Size: 416 53 83 399 24 852 1130 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1130 958 852 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2382 2466 2550 2634 2718 2886 4328
Size: 917 226 84 84 84 84 168 1442
Cuts at: 4328 1239
Size: 2579
Fragments arranged by size:
2579 1442 917 226 168 84 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2851 3459 493
Size: 711 1647 608 2702
Fragments arranged by size:
2702 1647 711 608
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 4758
BseYI C'CCAG_C
Cuts at: 4871 5367 4871
Size: 496 5172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2274 1163
Size: 573 538 4557
Fragments arranged by size:
4557 573 538
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2498 2514 2766 3729 3814 4885 2498
Size: 16 252 963 85 1071 3281
Fragments arranged by size:
3281 1071 963 252 85 16
BsmI GAATG_Cn'
Cuts at: 2384 2636 3179 3272 2384
Size: 252 543 93 4780
Fragments arranged by size:
4780 543 252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2498 2514 2766 2987 3729 3814 4885
Size: 399 708 16 252 221 742 85 1071
Cuts at: 4885 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 708 399 252 221 85
16
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3023 844
Size: 2179 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2090 4163 4337 65
Size: 1183 842 2073 174 1396
Fragments arranged by size:
2073 1396 1183 842 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2435 2998 3365 3750 3767 5219 5305 5317
Size: 463 563 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 563 463 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2840 2840
Size: 5668
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4177
Fragments arranged by size:
4177 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 2389 2641 3184 3978 3998 2070
Size: 319 252 543 794 20 3740
Fragments arranged by size:
3740 794 543 319 252 20
ClaI AT'CG_AT
Cuts at: 1554 2658 1554
Size: 1104 4564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2029 4675 809
Size: 507 713 2646 1802
Fragments arranged by size:
2646 1802 713 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2950 3115 4004
Size: 54 1425 50 275 18 1120 165 889
Cuts at: 4004 5633 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1120 889 275 165 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3598 1353
Size: 2245 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2496 2496
Size: 5668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2237 2382 2466 2550 2573 2634
Size: 393 524 81 145 84 84 23 61
Cuts at: 2634 2718 2722 2886 3731 4328 5115 5383 1239
Size: 84 4 164 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 597 524 393 268 164
145 84 84 84 81 61 23 4
EcoRI G'AATT_C
Cuts at: 2827 2977 3355 2827
Size: 150 378 5140
Fragments arranged by size:
5140 378 150
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2517 2651 2683 4309
Size: 32 135 32 913 32 134 32 1626
Cuts at: 4309 4341 4383 4415 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1626 913 135 134 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 2289 3267 2289
Size: 978 4690
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2952 10
Size: 54 1425 343 1120 2726
Fragments arranged by size:
2726 1425 1120 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2505 2781 3626 3985 4144 518
Size: 648 1339 276 845 359 159 2042
Fragments arranged by size:
2042 1339 845 648 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2215 3084 3761 4909 5154 1606
Size: 609 869 677 1148 245 2120
Fragments arranged by size:
2120 1148 869 677 609 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4177
Fragments arranged by size:
4177 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2600 3026 4571 1132
Size: 97 1371 426 1545 2229
Fragments arranged by size:
2229 1545 1371 426 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 2596 4567 2596
Size: 1971 3697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 1108
Size: 951 4717
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PmlI CAC'GTG
Cuts at: 2851 2851
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 3737 3769 5250 5282 2141
Size: 32 1564 32 1481 32 2527
Fragments arranged by size:
2527 1564 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 2880 2927 3238 2880
Size: 47 311 5310
Fragments arranged by size:
5310 311 47
PstI C_TGCA'G
Cuts at: 830 2014 2180 3020 830
Size: 1184 166 840 3478
Fragments arranged by size:
3478 1184 840 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2215 2215
Size: 5668
SacI G_AGCT'C
Cuts at: 2498 2498
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SexAI A'CCwGG_T
Cuts at: 2027 2027
Size: 5668
SfcI C'TryA_G
Cuts at: 826 1045 2010 2176 3016 4151 4832 5023 826
Size: 219 965 166 840 1135 681 191 1471
Fragments arranged by size:
1471 1135 965 840 681 219 191 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 2672 3772 4673 4935 820
Size: 197 926 477 252 1100 901 262 1553
Fragments arranged by size:
1553 1100 926 901 477 262 252 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 2819 1517
Size: 1302 4366
StyI C'CwwG_G
Cuts at: 513 1065 2004 2196 3107 513
Size: 552 939 192 911 3074
Fragments arranged by size:
3074 939 911 552 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 2184 1316
Size: 868 4800
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 2399 2567 3797 2399
Size: 168 1230 4270
Fragments arranged by size:
4270 1230 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI