Restriction map for
pFN21AB7629
(Circular) (Six-base) MAPSORT of: pFN21AB7629.seq Check: 3315 from: 1 to: 5662
pFN21AB7629 5662 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2303 2399 3725 5109 5377 1632
Size: 527 144 96 1326 1384 268 1917
Fragments arranged by size:
1917 1384 1326 527 268 144 96
AfeI AGC'GCT
Cuts at: 2670 2670
Size: 5662
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2687 1184
Size: 1503 4159
AlwNI CAG_nnn'CTG
Cuts at: 2339 3405 4977 2339
Size: 1066 1572 3024
Fragments arranged by size:
3024 1572 1066
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2069 2251 2417 2543 2971 3197 3349 5458
Size: 576 182 166 126 428 226 152 2109
Cuts at: 5458 1493
Size: 1697
Fragments arranged by size:
2109 1697 576 428 226 182 166 152
126
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2646 2987 1870
Size: 73 703 341 4545
Fragments arranged by size:
4545 703 341 73
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 4438 618
Size: 325 217 627 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2214 2536 2981 1391
Size: 823 322 445 4072
Fragments arranged by size:
4072 823 445 322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 2265 2867 928
Size: 971 248 118 602 3723
Fragments arranged by size:
3723 971 602 248 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2729 4273 136
Size: 107 122 71 631 605 1057 1544 1525
Fragments arranged by size:
1544 1525 1057 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 2660 3079 2660
Size: 419 5243
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4879 1790
Size: 1933 1156 2573
Fragments arranged by size:
2573 1933 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2587 3393 4351
Size: 416 53 83 399 24 1170 806 958
Cuts at: 4351 442
Size: 1753
Fragments arranged by size:
1753 1170 958 806 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2550 4322 1239
Size: 1172 139 1772 2579
Fragments arranged by size:
2579 1772 1172 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4325 882
Size: 617 2826 2219
Fragments arranged by size:
2826 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2864 3469 3851 4486
Size: 53 83 186 936 1331 605 382 635
Cuts at: 4486 275
Size: 1451
Fragments arranged by size:
1451 1331 936 635 605 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 1178
Size: 30 165 30 762 30 4645
Fragments arranged by size:
4645 762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5662
BseYI C'CCAG_C
Cuts at: 2855 4865 5361 2855
Size: 2010 496 3156
Fragments arranged by size:
3156 2010 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5089
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 3723 3808 4879 2214
Size: 1509 85 1071 2997
Fragments arranged by size:
2997 1509 1071 85
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2536 2981 3723 3808 4879 1391
Size: 399 424 322 445 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 445 424 399 322 85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3017 844
Size: 2173 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2059 4157 4331 65
Size: 1183 811 2098 174 1396
Fragments arranged by size:
2098 1396 1183 811 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2134 2833 2872 2992 3359 3744 3761 5213
Size: 162 699 39 120 367 385 17 1452
Cuts at: 5213 5299 5311 5657 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 699 385 367 346 162 120
86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2079 2079
Size: 5662
BtgI C'CryG_G
Cuts at: 513 1065 2587 513
Size: 552 1522 3588
Fragments arranged by size:
3588 1522 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2610 2966 2610
Size: 356 5306
DraIII CAC_nnn'GTG
Cuts at: 2161 2161
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2692 3109 3998
Size: 54 1425 50 275 18 862 417 889
Cuts at: 3998 5627 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 862 417 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 3592 1353
Size: 1125 1114 3423
Fragments arranged by size:
3423 1125 1114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2212 2212
Size: 5662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2303 2399 2411 2550 3725 4322
Size: 393 527 144 96 12 139 1175 597
Cuts at: 4322 5109 5377 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1175 787 597 527 393 268 144
139 96 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2078
Size: 5662
EcoRI G'AATT_C
Cuts at: 2069 2543 2971 3349 2069
Size: 474 428 378 4382
Fragments arranged by size:
4382 474 428 378
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 2646 2672 4809 1678
Size: 968 26 2137 2531
Fragments arranged by size:
2531 2137 968 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2523 2555 4303 4335 4377
Size: 32 135 32 951 32 1748 32 42
Cuts at: 4377 4409 1373
Size: 32 2626
Fragments arranged by size:
2626 1748 951 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3006 3252 669
Size: 988 281 1068 246 3079
Fragments arranged by size:
3079 1068 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2243 748
Size: 1495 4167
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 2505 3261 2505
Size: 756 4906
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2833 4776 4960 1305
Size: 1528 1943 184 2007
Fragments arranged by size:
2007 1943 1528 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2694 10
Size: 54 1425 343 862 2978
Fragments arranged by size:
2978 1425 862 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3620 3979 4138 518
Size: 648 2454 359 159 2042
Fragments arranged by size:
2454 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3078 3755 4903 5148 1606
Size: 1472 677 1148 245 2120
Fragments arranged by size:
2120 1472 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5110
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 2487 2678 3020 4565 1132
Size: 97 1258 191 342 1545 2229
Fragments arranged by size:
2229 1545 1258 342 191 97
PasI CC'CwG_GG
Cuts at: 1348 2167 1348
Size: 819 4843
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2577 1108
Size: 1469 4193
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
SacI G_AGCT'C
Cuts at: 2214 2214
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SapI GCTCTTCn'nnn_
Cuts at: 2478 2478
Size: 5662
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 3010 4145 4826 5017 826
Size: 219 1965 1135 681 191 1471
Fragments arranged by size:
1965 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3766 4667 4929 820
Size: 197 926 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 2487 3020 1132
Size: 1355 533 3774
Fragments arranged by size:
3774 1355 533
SspI AAT'ATT
Cuts at: 5 52 2910 3586 5
Size: 47 2858 676 2081
Fragments arranged by size:
2858 2081 676 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 3101 513
Size: 552 2036 3074
Fragments arranged by size:
3074 2036 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3689 3706 3859 4044 4489 325
Size: 1255 2109 17 153 185 445 1498
Fragments arranged by size:
2109 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3908 5620 96
Size: 274 80 33 51 1443 1931 1712 138
Fragments arranged by size:
1931 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2165 2197 5622 5654 2165
Size: 32 3425 32 2173
Fragments arranged by size:
3425 2173 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 2073 3791 2073
Size: 1718 3944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI