Restriction map for
pFN21AB7627
(Circular) (Six-base) MAPSORT of: pFN21AB7627.seq Check: 7471 from: 1 to: 5596
pFN21AB7627 5596 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2063 2063
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3659 5043 5311 1632
Size: 2027 1384 268 1917
Fragments arranged by size:
2027 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 2048 4325 2048
Size: 2277 3319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 3339 4911 3339
Size: 1572 4024
ApaLI G'TGCA_C
Cuts at: 2323 3653 4809 2323
Size: 1330 1156 3110
Fragments arranged by size:
3110 1330 1156
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2657 2921 1870
Size: 73 714 264 4545
Fragments arranged by size:
4545 714 264 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 4372 618
Size: 325 217 627 1130 1455 1842
Fragments arranged by size:
1842 1455 1130 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2475 2868 2915 1391
Size: 1084 393 47 4072
Fragments arranged by size:
4072 1084 393 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2327 2403 2877 3657 4813 1790
Size: 537 76 474 780 1156 2573
Fragments arranged by size:
2573 1156 780 537 474 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 2752 3327
Size: 416 53 83 399 24 621 714 575
Cuts at: 3327 4285 442
Size: 958 1753
Fragments arranged by size:
1753 958 714 621 575 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2832 2871 2882 4256 1239
Size: 1593 39 11 1374 2579
Fragments arranged by size:
2579 1593 1374 39 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2588 2813 4259 882
Size: 617 1089 225 1446 2219
Fragments arranged by size:
2219 1446 1089 617 225
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 1186
Size: 944 4652
BseYI C'CCAG_C
Cuts at: 2160 2585 2637 2888 4799 5295 2160
Size: 425 52 251 1911 496 2461
Fragments arranged by size:
2461 1911 496 425 251 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2110 2212 2704 1163
Size: 573 374 102 492 4055
Fragments arranged by size:
4055 573 492 374 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2327 2868 3657 3742 4813 2327
Size: 541 789 85 1071 3110
Fragments arranged by size:
3110 1071 789 541 85
BsmI GAATG_Cn'
Cuts at: 2768 3107 3200 2768
Size: 339 93 5164
Fragments arranged by size:
5164 339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2327 2403 2475 2868 2877 2915 3657
Size: 399 537 76 72 393 9 38 742
Cuts at: 3657 3742 4813 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 537 399 393 85 76
72 38 9
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2063 2136 2787 2951 844
Size: 1219 73 651 164 3489
Fragments arranged by size:
3489 1219 651 164 73
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2509 4091 4265 65
Size: 1183 1261 1582 174 1396
Fragments arranged by size:
1582 1396 1261 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2133 2322 794
Size: 1339 189 4068
Fragments arranged by size:
4068 1339 189
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstXI CCAn_nnnn'nTGG
Cuts at: 2066 2066
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591
Size: 954 367 385 17 1452 86 12 346
Cuts at: 5591 1972
Size: 1977
Fragments arranged by size:
1977 1452 954 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2406 2406
Size: 5596
BtgI C'CryG_G
Cuts at: 513 1065 2004 2709 513
Size: 552 939 705 3400
Fragments arranged by size:
3400 939 705 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3906 3926 3112
Size: 794 20 4782
Fragments arranged by size:
4782 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2077 2112 2404 2439 529
Size: 35 1513 35 292 35 3686
Fragments arranged by size:
3686 1513 292 35 35 35
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3043 3932 5561
Size: 54 1425 50 275 18 1213 889 1629
Cuts at: 5561 8
Size: 43
Fragments arranged by size:
1629 1425 1213 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 2576 3526 1353
Size: 1127 96 950 3423
Fragments arranged by size:
3423 1127 950 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2866 2866
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2832 2871 2882 3659 4256 5043 5311
Size: 393 1200 39 11 777 597 787 268
Cuts at: 5311 1239
Size: 1524
Fragments arranged by size:
1524 1200 787 777 597 393 268 39
11
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 5596
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 2657 2689 4237
Size: 32 135 32 462 32 591 32 1548
Cuts at: 4237 4269 4311 4343 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1548 591 462 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 2940 3186 669
Size: 988 281 286 716 246 3079
Fragments arranged by size:
3079 988 716 286 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3554 3913 4072 518
Size: 648 2388 359 159 2042
Fragments arranged by size:
2388 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 3012 3689 4837 5082 1606
Size: 554 852 677 1148 245 2120
Fragments arranged by size:
2120 1148 852 677 554 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2709 513
Size: 552 939 705 3400
Fragments arranged by size:
3400 939 705 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PfoI T'CCnGG_A
Cuts at: 2071 2071
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PspXI vC'TCGA_Gb
Cuts at: 2657 2657
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2304 2535 2672 2948 830
Size: 1474 231 137 276 3478
Fragments arranged by size:
3478 1474 276 231 137
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2160 2160
Size: 5596
SacI G_AGCT'C
Cuts at: 2868 2868
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2480 2480
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2304 2948 2304
Size: 644 4952
ScaI AGT'ACT
Cuts at: 2027 3844 5556 2027
Size: 1817 1712 2067
Fragments arranged by size:
2067 1817 1712
SfcI C'TryA_G
Cuts at: 826 1045 2300 2531 2668 2944 4079 4760 4951
Size: 219 1255 231 137 276 1135 681 191
Cuts at: 4951 826
Size: 1471
Fragments arranged by size:
1471 1255 1135 681 276 231 219 191
137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 3700 4601 4863 820
Size: 197 926 714 1043 901 262 1553
Fragments arranged by size:
1553 1043 926 901 714 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 2678 152
Size: 2526 3070
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 2298 1517
Size: 781 4815
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2709 2823 3035 513
Size: 552 939 84 621 114 212 3074
Fragments arranged by size:
3074 939 621 552 212 114 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2407 2599 3623 3640 3793 3978 4423
Size: 1255 827 192 1024 17 153 185 445
Cuts at: 4423 325
Size: 1498
Fragments arranged by size:
1498 1255 1024 827 445 192 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 3842 5554
Size: 274 80 33 51 1443 48 1817 1712
Cuts at: 5554 96
Size: 138
Fragments arranged by size:
1817 1712 1443 274 138 80 51 48
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2297 2329 5556 5588 2297
Size: 32 3227 32 2305
Fragments arranged by size:
3227 2305 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 2657 1943
Size: 714 4882
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI