Restriction map for
pFN21AB7623
(Circular) (Six-base) MAPSORT of: pFN21AB7623.seq Check: 5942 from: 1 to: 5647
pFN21AB7623 5647 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2823 2990 1937
Size: 886 167 4594
Fragments arranged by size:
4594 886 167
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 5094 5362 1632
Size: 2078 1384 268 1917
Fragments arranged by size:
2078 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 2523 2832 2928 4546 5523 1203
Size: 1320 309 96 1618 977 1327
Fragments arranged by size:
1618 1327 1320 977 309 96
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2461 3390 4962 2461
Size: 929 1572 3146
Fragments arranged by size:
3146 1572 929
ApaI G_GGCC'C
Cuts at: 2091 2205 2696 2912 2091
Size: 114 491 216 4826
Fragments arranged by size:
4826 491 216 114
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 2204 2722 2842 2917 2972 1870
Size: 73 189 72 518 120 75 55 4545
Fragments arranged by size:
4545 518 189 120 75 73 72 55
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2572 2699 2968 4423 618
Size: 325 217 627 785 127 269 1455 1842
Fragments arranged by size:
1842 1455 785 627 325 269 217 127
BanII G_rGCy'C
Cuts at: 1391 2055 2091 2205 2624 2696 2912 2966 1391
Size: 664 36 114 419 72 216 54 4072
Fragments arranged by size:
4072 664 419 216 114 72 54 36
BbeI G_GCGC'C
Cuts at: 2576 2703 2576
Size: 127 5520
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 2796 2907
Size: 107 122 71 631 605 378 746 111
Cuts at: 2907 4258 136
Size: 1351 1525
Fragments arranged by size:
1525 1351 746 631 605 378 122 111
107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2205 2696 2912 3708 4864 1790
Size: 301 114 491 216 796 1156 2573
Fragments arranged by size:
2573 1156 796 491 301 216 114
BmgBI CAC'GTC
Cuts at: 1318 2524 1318
Size: 1206 4441
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 3378 4336
Size: 416 53 83 399 24 1146 815 958
Cuts at: 4336 442
Size: 1753
Fragments arranged by size:
1753 1146 958 815 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 4307 1239
Size: 815 2253 2579
Fragments arranged by size:
2579 2253 815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3772 4637 4935 2849
Size: 923 865 298 3561
Fragments arranged by size:
3561 923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2485 2835 3438 493
Size: 711 1281 350 603 2702
Fragments arranged by size:
2702 1281 711 603 350
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2573 2700 3454
Size: 53 83 186 936 477 563 127 754
Cuts at: 3454 3836 4471 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 754 635 563 477 382 186
127 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2656 2746 1186
Size: 1192 278 90 4087
Fragments arranged by size:
4087 1192 278 90
BseYI C'CCAG_C
Cuts at: 2251 2654 4850 5346 2251
Size: 403 2196 496 2552
Fragments arranged by size:
2552 2196 496 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 1163
Size: 573 792 4282
Fragments arranged by size:
4282 792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2060 3097 3858 4886 1540
Size: 275 186 59 1037 761 1028 2301
Fragments arranged by size:
2301 1037 1028 761 275 186 59
BsiHKAI G_wGCw'C
Cuts at: 3708 3793 4864 3708
Size: 85 1071 4491
Fragments arranged by size:
4491 1071 85
BsiWI C'GTAC_G
Cuts at: 2282 2793 2282
Size: 511 5136
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2091 2205 2624 2696 2912 2966
Size: 399 265 36 114 419 72 216 54
Cuts at: 2966 3708 3793 4864 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 399 265 216 114
85 72 54 36
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 844
Size: 2158 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 2131 2584 3534 1394
Size: 429 215 93 453 950 3507
Fragments arranged by size:
3507 950 453 429 215 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2534 2750 4291 4478 1700
Size: 110 724 216 1541 187 2869
Fragments arranged by size:
2869 1541 724 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2232 794
Size: 1438 4209
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2569 2569
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642
Size: 1005 367 385 17 1452 86 12 346
Cuts at: 5642 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 3163 3957 3977 3163
Size: 794 20 4833
Fragments arranged by size:
4833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2680 4654 809
Size: 507 1364 1974 1802
Fragments arranged by size:
1974 1802 1364 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2057 2769 3094
Size: 54 1425 50 275 18 227 712 325
Cuts at: 3094 3983 5612 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 712 325 275 227 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2057 3094 1537
Size: 275 245 1037 4090
Fragments arranged by size:
4090 1037 275 245
EarI CTCTTCn'nnn_
Cuts at: 1353 3577 1353
Size: 2224 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2131 4234 4608 4754 387
Size: 1064 138 542 2103 374 146 1280
Fragments arranged by size:
2103 1280 1064 542 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 3710 4307 5094 5362 1239
Size: 393 422 1656 597 787 268 1524
Fragments arranged by size:
1656 1524 787 597 422 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2471
Size: 5647
EcoO109I rG'GnC_Cy
Cuts at: 2087 2201 2908 2087
Size: 114 707 4826
Fragments arranged by size:
4826 707 114
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2576 2582 2703 2787 4794 1678
Size: 898 6 121 84 2007 2531
Fragments arranged by size:
2531 2007 898 121 84 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373
Size: 32 135 32 2716 32 42 32 2626
Fragments arranged by size:
2716 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KasI G'GCGC_C
Cuts at: 2572 2699 2572
Size: 127 5520
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 2928 5523 2928
Size: 2595 3052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2675 3605 3964 4123 518
Size: 648 1188 321 930 359 159 2042
Fragments arranged by size:
2042 1188 930 648 359 321 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2461 2499 2626 3063 3740 4888 5133
Size: 666 189 38 127 437 677 1148 245
Cuts at: 5133 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 666 437 245 189 127
38
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 5647
NarI GG'CG_CC
Cuts at: 2573 2700 2573
Size: 127 5520
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2716 3005 4550 1132
Size: 97 1487 289 1545 2229
Fragments arranged by size:
2229 1545 1487 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 2461 1108
Size: 975 378 4294
Fragments arranged by size:
4294 975 378
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PmlI CAC'GTG
Cuts at: 2835 2835
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2309 2341 3716 3748 5229 5261 2309
Size: 32 1375 32 1481 32 2695
Fragments arranged by size:
2695 1481 1375 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2087 2201 2692 2908 2087
Size: 114 491 216 4826
Fragments arranged by size:
4826 491 216 114
PspXI vC'TCGA_Gb
Cuts at: 2842 2842
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2907 2999 830
Size: 2077 92 3478
Fragments arranged by size:
3478 2077 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2907 2999 2907
Size: 92 5555
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2815 2903 2995 4130 4811 5002 826
Size: 219 1770 88 92 1135 681 191 1471
Fragments arranged by size:
1770 1471 1135 681 219 191 92 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 2907 2907
Size: 5647
SfoI GGC'GCC
Cuts at: 2574 2701 2574
Size: 127 5520
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2842 2864 3751 4652 4914
Size: 197 926 189 710 22 887 901 262
Cuts at: 4914 820
Size: 1553
Fragments arranged by size:
1553 926 901 887 710 262 197 189
22
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 2716 3005 1132
Size: 1584 289 3774
Fragments arranged by size:
3774 1584 289
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2004 2196 3086 513
Size: 552 939 192 890 3074
Fragments arranged by size:
3074 939 890 552 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2668 3674 3691 3844 4029 4474 325
Size: 1255 1088 1006 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1088 1006 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 3893 5605
Size: 274 80 33 51 1443 960 956 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1443 960 956 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 2132 2842 1943
Size: 189 710 4748
Fragments arranged by size:
4748 710 189
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PpuMI
PsrI RsrII SacI SacII SanDI SapI SexAI SrfI
SwaI XcmI