Restriction map for
pFN21AB7622
(Circular) (Six-base) MAPSORT of: pFN21AB7622.seq Check: 5265 from: 1 to: 5809
pFN21AB7622 5809 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2437 3152 1937
Size: 500 715 4594
Fragments arranged by size:
4594 715 500
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2610 2767 3872 5256 5524 1632
Size: 978 157 1105 1384 268 1917
Fragments arranged by size:
1917 1384 1105 978 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 2422 4640 2422
Size: 2218 3591
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2322 1184
Size: 1138 4671
AlwNI CAG_nnn'CTG
Cuts at: 3552 5124 3552
Size: 1572 4237
ApaI G_GGCC'C
Cuts at: 2202 2202
Size: 5809
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 2070 2679 2817 2959 3118 3344 3496 5605
Size: 577 609 138 142 159 226 152 2109
Cuts at: 5605 1493
Size: 1697
Fragments arranged by size:
2109 1697 609 577 226 159 152 142
138
AseI AT'TA_AT
Cuts at: 160 1032 2973 4363 160
Size: 872 1941 1390 1606
Fragments arranged by size:
1941 1606 1390 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 3134 1870
Size: 73 1191 4545
Fragments arranged by size:
4545 1191 73
BamHI G'GATC_C
Cuts at: 2095 3139 3506 2095
Size: 1044 367 4398
Fragments arranged by size:
4398 1044 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3130 4585 618
Size: 325 217 627 1343 1455 1842
Fragments arranged by size:
1842 1455 1343 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2553 2795 3128 1391
Size: 811 351 242 333 4072
Fragments arranged by size:
4072 811 351 333 242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2469 3076 928
Size: 971 570 607 3661
Fragments arranged by size:
3661 971 607 570
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2214 2248 4000 4034 1903
Size: 34 277 34 1752 34 3678
Fragments arranged by size:
3678 1752 277 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2132 2174 3701 4911 2132
Size: 42 1527 1210 3030
Fragments arranged by size:
3030 1527 1210 42
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4420 136
Size: 107 122 71 631 605 2748 1525
Fragments arranged by size:
2748 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5804 5804
Size: 5809
BlpI GC'TnA_GC
Cuts at: 2794 3226 2794
Size: 432 5377
Bme1580I G_kGCm'C
Cuts at: 1790 2202 3870 5026 1790
Size: 412 1668 1156 2573
Fragments arranged by size:
2573 1668 1156 412
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3003 3540 4498
Size: 416 53 83 399 24 1586 537 958
Cuts at: 4498 442
Size: 1753
Fragments arranged by size:
1753 1586 958 537 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2315 2405 2931 4469 1239
Size: 798 278 90 526 1538 2579
Fragments arranged by size:
2579 1538 798 526 278 90
Bpu10I CC'TnA_GC
Cuts at: 2246 2487 2246
Size: 241 5568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2460 2516 3934 4799 5097 2460
Size: 56 1418 865 298 3172
Fragments arranged by size:
3172 1418 865 298 56
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4472 882
Size: 617 2973 2219
Fragments arranged by size:
2973 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 2349 5803 2349
Size: 3454 2355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3616 3998 4633 275
Size: 53 83 186 936 2083 382 635 1451
Fragments arranged by size:
2083 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2422 4242 4640 4914 5061 5472 2422
Size: 1820 398 274 147 411 2759
Fragments arranged by size:
2759 1820 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2381 1186
Size: 919 276 4614
Fragments arranged by size:
4614 919 276
BseYI C'CCAG_C
Cuts at: 5012 5508 5012
Size: 496 5313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1995 1163
Size: 573 259 4977
Fragments arranged by size:
4977 573 259
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 4020 5048 1540
Size: 275 186 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 2602 3870 3955 5026 2553
Size: 49 1268 85 1071 3336
Fragments arranged by size:
3336 1268 1071 85 49
BsmI GAATG_Cn'
Cuts at: 2864 3017 3320 3413 2864
Size: 153 303 93 5260
Fragments arranged by size:
5260 303 153 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2249 2249
Size: 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2553 2602 2795 3128 3870 3955
Size: 399 412 351 49 193 333 742 85
Cuts at: 3955 5026 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 351 333 193
85 49
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 3164 844
Size: 1862 458 3489
Fragments arranged by size:
3489 1862 458
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 1394
Size: 429 1873 3507
Fragments arranged by size:
3507 1873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4304 4478 65
Size: 1183 3056 174 1396
Fragments arranged by size:
3056 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2422 4453 4640 1700
Size: 110 612 2031 187 2869
Fragments arranged by size:
2869 2031 612 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 3869 4881 3869
Size: 1012 4797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 2095 3139 3506 3891 3908 5360 5446 5458
Size: 123 1044 367 385 17 1452 86 12
Cuts at: 5458 5804 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1044 385 367 346 123 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2561 2561
Size: 5809
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2379 3325 4119 4139 2379
Size: 946 794 20 4049
Fragments arranged by size:
4049 946 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 2686 3113 2686
Size: 427 5382
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3256 4145 5774
Size: 54 1425 50 275 18 1426 889 1629
Cuts at: 5774 8
Size: 43
Fragments arranged by size:
1629 1426 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2103 3739 1353
Size: 702 48 1636 3423
Fragments arranged by size:
3423 1636 702 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4396 4770 4916 387
Size: 1064 138 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 2551
Size: 5809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2315 2405 2610 2767 2931 3872
Size: 393 405 278 90 205 157 164 941
Cuts at: 3872 4469 5256 5524 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 941 787 597 405 393 278 268
205 164 157 90
EcoO109I rG'GnC_Cy
Cuts at: 2199 2199
Size: 5809
EcoRI G'AATT_C
Cuts at: 2817 2959 3118 3496 2817
Size: 142 159 378 5130
Fragments arranged by size:
5130 378 159 142
FspI TGC'GCA
Cuts at: 4315 4315
Size: 5809
HaeII r_GCGC'y
Cuts at: 1678 4956 1678
Size: 3278 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2456 2488 4450
Size: 32 135 32 534 32 318 32 1962
Cuts at: 4450 4482 4524 4556 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1962 534 318 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2438 3153 3399 669
Size: 988 281 500 715 246 3079
Fragments arranged by size:
3079 988 715 500 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 2182 4923 5107 1305
Size: 762 115 2741 184 2007
Fragments arranged by size:
2741 2007 762 184 115
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3987
Fragments arranged by size:
3987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2571 2760 2885 3767 4126 4285 518
Size: 648 1405 189 125 882 359 159 2042
Fragments arranged by size:
2042 1405 882 648 359 189 159 125
MspA1I CmG'CkG
Cuts at: 1606 3225 3902 5050 5295 1606
Size: 1619 677 1148 245 2120
Fragments arranged by size:
2120 1619 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5257
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 3167 4712 1132
Size: 97 1938 1545 2229
Fragments arranged by size:
2229 1938 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 1108
Size: 1252 4557
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PspOMI G'GGCC_C
Cuts at: 2198 2198
Size: 5809
PstI C_TGCA'G
Cuts at: 830 3161 830
Size: 2331 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
SacI G_AGCT'C
Cuts at: 2553 2553
Size: 5809
SalI G'TCGA_C
Cuts at: 1936 2436 3151 1936
Size: 500 715 4594
Fragments arranged by size:
4594 715 500
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 2454 4057 5769 2454
Size: 1603 1712 2494
Fragments arranged by size:
2494 1712 1603
SfcI C'TryA_G
Cuts at: 826 1045 2327 3157 4292 4973 5164 826
Size: 219 1282 830 1135 681 191 1471
Fragments arranged by size:
1471 1282 1135 830 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2475 2531 3913 4814 5076 820
Size: 197 926 532 56 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 901 532 262 197 56
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 2455 152
Size: 2303 3506
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5809
StyI C'CwwG_G
Cuts at: 513 1065 2709 3248 513
Size: 552 1644 539 3074
Fragments arranged by size:
3074 1644 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2657 3836 3853 4006 4191 4636 325
Size: 1255 1077 1179 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1179 1077 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2452 4055 5767
Size: 274 80 33 51 1443 475 1603 1712
Cuts at: 5767 96
Size: 138
Fragments arranged by size:
1712 1603 1443 475 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5769 5801 5769
Size: 32 5777
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2648 2192
Size: 456 5353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 2070 2864 3015 3087 3938 2070
Size: 794 151 72 851 3941
Fragments arranged by size:
3941 851 794 151 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI