Restriction map for
pFN21AB7616
(Circular) (Six-base) MAPSORT of: pFN21AB7616.seq Check: 7397 from: 1 to: 5788
pFN21AB7616 5788 bp
With 182 enzymes: SgfI *
March 28, 2012 12:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2529 3598 278
Size: 53 83 186 936 993 1069 2468
Fragments arranged by size:
2468 1069 993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2400 3851 5235 5503 1632
Size: 768 1451 1384 268 1917
Fragments arranged by size:
1917 1451 1384 768 268
AfeI AGC'GCT
Cuts at: 2028 2160 2028
Size: 132 5656
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 2960 1166
Size: 1794 3994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2942 2978 1184
Size: 1758 36 3994
Fragments arranged by size:
3994 1758 36
AlwNI CAG_nnn'CTG
Cuts at: 2833 3531 5103 2833
Size: 698 1572 3518
Fragments arranged by size:
3518 1572 698
ApaI G_GGCC'C
Cuts at: 2247 2873 2247
Size: 626 5162
ApaLI G'TGCA_C
Cuts at: 2089 3845 5001 2089
Size: 1756 1156 2876
Fragments arranged by size:
2876 1756 1156
ApoI r'AATT_y
Cuts at: 1493 3097 3323 3475 5584 1493
Size: 1604 226 152 2109 1697
Fragments arranged by size:
2109 1697 1604 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2137 2177 2246 2535 2702 2813
Size: 73 125 69 40 69 289 167 111
Cuts at: 2813 3113 1870
Size: 300 4545
Fragments arranged by size:
4545 300 289 167 125 111 73 69
69 40
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 3109 4564 618
Size: 325 217 627 288 1034 1455 1842
Fragments arranged by size:
1842 1455 1034 627 325 288 217
BanII G_rGCy'C
Cuts at: 1391 2136 2144 2247 2256 2667 2873 3107 1391
Size: 745 8 103 9 411 206 234 4072
Fragments arranged by size:
4072 745 411 234 206 103 9 8
BbeI G_GCGC'C
Cuts at: 2079 2079
Size: 5788
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2577 3045 928
Size: 971 678 468 3671
Fragments arranged by size:
3671 971 678 468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2080 2114 2781 2815 3979 4013 1903
Size: 34 143 34 667 34 1164 34 3678
Fragments arranged by size:
3678 1164 667 143 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3680 4890 2571
Size: 1109 1210 3469
Fragments arranged by size:
3469 1210 1109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2251 4399 136
Size: 107 122 71 631 605 579 2148 1525
Fragments arranged by size:
2148 1525 631 605 579 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 2030 2207 3205 2030
Size: 177 998 4613
Fragments arranged by size:
4613 998 177
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2184 2247 2752 2873 3849 5005 1790
Size: 303 91 63 505 121 976 1156 2573
Fragments arranged by size:
2573 1156 976 505 303 121 91 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2103 2771 3519
Size: 416 53 83 399 24 686 668 748
Cuts at: 3519 4477 442
Size: 958 1753
Fragments arranged by size:
1753 958 748 686 668 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2377 2513 2804 3095 4448 1239
Size: 1138 136 291 291 1353 2579
Fragments arranged by size:
2579 1353 1138 291 291 136
Bpu10I CC'TnA_GC
Cuts at: 2880 2943 2880
Size: 63 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 3913 4778 5076 2699
Size: 1214 865 298 3411
Fragments arranged by size:
3411 1214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2526 3595 3977
Size: 53 83 186 936 543 450 1069 382
Cuts at: 3977 4612 275
Size: 635 1451
Fragments arranged by size:
1451 1069 936 635 543 450 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2501 3032 3035 3038 1186
Size: 1315 531 3 3 3936
Fragments arranged by size:
3936 1315 531 3 3
BseYI C'CCAG_C
Cuts at: 2631 2965 4991 5487 2631
Size: 334 2026 496 2932
Fragments arranged by size:
2932 2026 496 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2401 3238 3999 5027 1540
Size: 275 186 400 837 761 1028 2301
Fragments arranged by size:
2301 1028 837 761 400 275 186
BsiHKAI G_wGCw'C
Cuts at: 2093 2144 2208 2931 2952 3849 3934 5005 2093
Size: 51 64 723 21 897 85 1071 2876
Fragments arranged by size:
2876 1071 897 723 85 64 51 21
BsmI GAATG_Cn'
Cuts at: 2501 3299 3392 2501
Size: 798 93 4897
Fragments arranged by size:
4897 798 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2136 2144 2184 2208 2247 2256
Size: 399 303 43 8 40 24 39 9
Cuts at: 2256 2667 2752 2873 2931 2952 3107 3849 3934
Size: 411 85 121 58 21 155 742 85
Cuts at: 3934 5005 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 303 155 121
85 85 58 43 40 39 24 21
9 8
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 844
Size: 2299 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2866 3675 1394
Size: 429 1043 809 3507
Fragments arranged by size:
3507 1043 809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2919 4283 4457 65
Size: 1183 1671 1364 174 1396
Fragments arranged by size:
1671 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3046 4432 4619 1700
Size: 110 1236 1386 187 2869
Fragments arranged by size:
2869 1386 1236 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 2195 1925
Size: 270 5518
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2965 2284
Size: 681 5107
BstYI r'GATC_y
Cuts at: 1972 2112 3118 3485 3870 3887 5339 5425 5437
Size: 140 1006 367 385 17 1452 86 12
Cuts at: 5437 5783 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1006 385 367 346 140 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2150 2214 2676 2753 3030 513
Size: 552 1085 64 462 77 277 3271
Fragments arranged by size:
3271 1085 552 462 277 77 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2650 3304 4098 4118 2650
Size: 654 794 20 4320
Fragments arranged by size:
4320 794 654 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 2424 3092 2424
Size: 668 5120
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2082 2364
Size: 54 1425 50 275 18 225 27 282
Cuts at: 2364 2547 2886 3235 4124 5753 8
Size: 183 339 349 889 1629 43
Fragments arranged by size:
1629 1425 889 349 339 282 275 225
183 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3718 1353
Size: 2365 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2669 3065 4375 4749 4895 387
Size: 1064 138 1080 396 1310 374 146 1280
Fragments arranged by size:
1310 1280 1080 1064 396 374 146 138
EcoICRI GAG'CTC
Cuts at: 2142 2142
Size: 5788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2377 2400 2513 2804 3095 3851 4448
Size: 393 745 23 113 291 291 756 597
Cuts at: 4448 5235 5503 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 756 745 597 393 291 291
268 113 23
EcoO109I rG'GnC_Cy
Cuts at: 2243 2830 2869 2870 2243
Size: 587 39 1 5161
Fragments arranged by size:
5161 587 39 1
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2030 2079 2162 4935 1678
Size: 352 49 83 2773 2531
Fragments arranged by size:
2773 2531 352 83 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 3046 3078 4429
Size: 32 135 32 803 32 639 32 1351
Cuts at: 4429 4461 4503 4535 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1351 803 639 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KasI G'GCGC_C
Cuts at: 2075 2075
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 4902 5086 1305
Size: 1017 2580 184 2007
Fragments arranged by size:
2580 2007 1017 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2549 10
Size: 54 1425 343 225 492 3249
Fragments arranged by size:
3249 1425 492 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2924 2960 3746 4105 4264 518
Size: 648 1758 36 786 359 159 2042
Fragments arranged by size:
2042 1758 786 648 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 2152 2216 2347 3073 3204 3881 5029 5274
Size: 546 64 131 726 131 677 1148 245
Cuts at: 5274 1606
Size: 2120
Fragments arranged by size:
2120 1148 726 677 546 245 131 131
64
NaeI GCC'GGC
Cuts at: 3048 3048
Size: 5788
NarI GG'CG_CC
Cuts at: 2076 2076
Size: 5788
NcoI C'CATG_G
Cuts at: 513 1065 2676 3030 513
Size: 552 1611 354 3271
Fragments arranged by size:
3271 1611 552 354
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NgoMIV G'CCGG_C
Cuts at: 3046 3046
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PfoI T'CCnGG_A
Cuts at: 2106 2283 2106
Size: 177 5611
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2243 2869 2243
Size: 626 5162
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2401 2001
Size: 400 5388
PvuII CAG'CTG
Cuts at: 3073 3073
Size: 5788
SacI G_AGCT'C
Cuts at: 2144 2144
Size: 5788
SacII CC_GC'GG
Cuts at: 2153 2217 2153
Size: 64 5724
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 3136 4271 4952 5143 826
Size: 219 2091 1135 681 191 1471
Fragments arranged by size:
2091 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2077 2077
Size: 5788
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 2179 2248 2537 3115 1872
Size: 307 69 289 578 4545
Fragments arranged by size:
4545 578 307 289 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2714 3892 4793 5055 820
Size: 197 926 771 1178 901 262 1553
Fragments arranged by size:
1553 1178 926 901 771 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2676 2724 3030 3227 513
Size: 552 1611 48 306 197 3074
Fragments arranged by size:
3074 1611 552 306 197 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 2974 1316
Size: 1658 4130
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2177 2246 2535 3113 1870
Size: 307 69 289 578 4545
Fragments arranged by size:
4545 578 307 289 69
XmnI GAAnn'nnTTC
Cuts at: 2501 3917 2501
Size: 1416 4372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2527 3596 276
Size: 53 83 186 936 993 1069 2468
Fragments arranged by size:
2468 1069 993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI
XcmI