Restriction map for
pFN21AB7613
(Circular) (Six-base) MAPSORT of: pFN21AB7613.seq Check: 3960 from: 1 to: 5614
pFN21AB7613 5614 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2235 3424 278
Size: 53 83 186 936 699 1189 2468
Fragments arranged by size:
2468 1189 936 699 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2292 2957 1937
Size: 355 665 4594
Fragments arranged by size:
4594 665 355
Acc65I G'GTAC_C
Cuts at: 2541 2785 2935 2541
Size: 244 150 5220
Fragments arranged by size:
5220 244 150
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 2679 3677 5061 5329 1632
Size: 815 232 998 1384 268 1917
Fragments arranged by size:
1917 1384 998 815 268 232
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 2592 4513 5490 1203
Size: 1389 1921 977 1327
Fragments arranged by size:
1921 1389 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 2033 2818 3357 4929 2033
Size: 785 539 1572 2718
Fragments arranged by size:
2718 1572 785 539
ApaI G_GGCC'C
Cuts at: 2766 2766
Size: 5614
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2923 3149 3301 5410 1493
Size: 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2721 2939 1870
Size: 73 778 218 4545
Fragments arranged by size:
4545 778 218 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2525 2558 2525
Size: 33 5581
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2323 2541 2785 2935
Size: 325 217 627 261 275 218 244 150
Cuts at: 2935 4390 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 275 261 244 218
217 150
BanII G_rGCy'C
Cuts at: 1391 2551 2766 2872 2933 1391
Size: 1160 215 106 61 4072
Fragments arranged by size:
4072 1160 215 106 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2740 3506 4716 2740
Size: 766 1210 3638
Fragments arranged by size:
3638 1210 766
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4225 136
Size: 107 122 71 631 605 2553 1525
Fragments arranged by size:
2553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2422 5609 2422
Size: 3187 2427
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2766 3675 4831 1790
Size: 976 909 1156 2573
Fragments arranged by size:
2573 1156 976 909
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2765 3345 4303
Size: 416 53 83 399 24 1348 580 958
Cuts at: 4303 442
Size: 1753
Fragments arranged by size:
1753 1348 958 580 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2688 4274 1239
Size: 974 475 1586 2579
Fragments arranged by size:
2579 1586 974 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 3739 4604 4902 2090
Size: 1649 865 298 2802
Fragments arranged by size:
2802 1649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4277 882
Size: 617 2778 2219
Fragments arranged by size:
2778 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 3421 3803 4438
Size: 53 83 186 936 699 1189 382 635
Cuts at: 4438 275
Size: 1451
Fragments arranged by size:
1451 1189 936 699 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2908 4047 4445 4719 4866 5277 2908
Size: 1139 398 274 147 411 3245
Fragments arranged by size:
3245 1139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2145 2148 2157 2813 1186
Size: 959 3 9 656 3987
Fragments arranged by size:
3987 959 656 9 3
BseYI C'CCAG_C
Cuts at: 4817 5313 4817
Size: 496 5118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2795 1163
Size: 573 1059 3982
Fragments arranged by size:
3982 1059 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2551 2812 2872 3675 3760 4831 2551
Size: 261 60 803 85 1071 3334
Fragments arranged by size:
3334 1071 803 261 85 60
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2551 2766 2812 2872 2933 3675 3760
Size: 399 761 215 46 60 61 742 85
Cuts at: 3760 4831 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 761 742 399 215 85 61
60 46
BspEI T'CCGG_A
Cuts at: 2908 5277 2908
Size: 2369 3245
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2969 844
Size: 2125 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4109 4283 65
Size: 1183 2861 174 1396
Fragments arranged by size:
2861 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4258 4445 1700
Size: 110 2448 187 2869
Fragments arranged by size:
2869 2448 187 110
BsrGI T'GTAC_A
Cuts at: 96 2064 96
Size: 1968 3646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 2269 1184
Size: 1085 4529
BstYI r'GATC_y
Cuts at: 1972 2359 2422 2944 3311 3696 3713 5165 5251
Size: 387 63 522 367 385 17 1452 86
Cuts at: 5251 5263 5609 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 522 387 385 367 346 86
63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2303
Size: 5614
BtgI C'CryG_G
Cuts at: 513 1065 2004 2523 2740 513
Size: 552 939 519 217 3387
Fragments arranged by size:
3387 939 552 519 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2319 2354 2568 2603 529
Size: 35 1755 35 214 35 3540
Fragments arranged by size:
3540 1755 214 35 35 35
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DraIII CAC_nnn'GTG
Cuts at: 2098 2098
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2487 3061
Size: 54 1425 50 275 18 259 398 574
Cuts at: 3061 3950 5579 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 574 398 275 259 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2648 3544 1353
Size: 1295 896 3423
Fragments arranged by size:
3423 1295 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2549 2870 2549
Size: 321 5293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2447 2679 2688 3677 4274 5061
Size: 393 581 234 232 9 989 597 787
Cuts at: 5061 5329 1239
Size: 268 1524
Fragments arranged by size:
1524 989 787 597 581 393 268 234
232 9
EcoO109I rG'GnC_Cy
Cuts at: 2299 2762 2763 2299
Size: 463 1 5150
Fragments arranged by size:
5150 463 1
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 5614
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 4761 1678
Size: 3083 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 2614 2646 4255
Size: 32 135 32 772 32 238 32 1609
Cuts at: 4255 4287 4329 4361 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1609 772 238 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2545 2789 2939 2545
Size: 244 150 5220
Fragments arranged by size:
5220 244 150
MfeI C'AATT_G
Cuts at: 2404 3213 2404
Size: 809 4805
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2091 2489 10
Size: 54 1425 343 259 398 3135
Fragments arranged by size:
3135 1425 398 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 2444 2895 3572 3931 4090 518
Size: 648 1045 233 451 677 359 159 2042
Fragments arranged by size:
2042 1045 677 648 451 359 233 159
MspA1I CmG'CkG
Cuts at: 1606 2815 3030 3707 4855 5100 1606
Size: 1209 215 677 1148 245 2120
Fragments arranged by size:
2120 1209 1148 677 245 215
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4123
Fragments arranged by size:
4123 939 552
NdeI CA'TA_TG
Cuts at: 387 2575 387
Size: 2188 3426
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2291 2596 2972 4517 1132
Size: 97 1062 305 376 1545 2229
Fragments arranged by size:
2229 1545 1062 376 305 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 2592 4513 2592
Size: 1921 3693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2098 1108
Size: 990 4624
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2299
Size: 5614
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PspOMI G'GGCC_C
Cuts at: 2762 2762
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2568 2877 2966 830
Size: 1738 309 89 3478
Fragments arranged by size:
3478 1738 309 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
PvuII CAG'CTG
Cuts at: 2815 2815
Size: 5614
SacI G_AGCT'C
Cuts at: 2551 2872 2551
Size: 321 5293
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2497 2564 2873 2962 4097 4778 4969
Size: 219 1452 67 309 89 1135 681 191
Cuts at: 4969 826
Size: 1471
Fragments arranged by size:
1471 1452 1135 681 309 219 191 89
67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 2721 3718 4619 4881 820
Size: 197 926 162 616 997 901 262 1553
Fragments arranged by size:
1553 997 926 901 616 262 197 162
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 2148 2385 1517
Size: 631 237 4746
Fragments arranged by size:
4746 631 237
StyI C'CwwG_G
Cuts at: 513 1065 2004 3053 513
Size: 552 939 1049 3074
Fragments arranged by size:
3074 1049 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3641 3658 3811 3996 4441 325
Size: 1255 2061 17 153 185 445 1498
Fragments arranged by size:
2061 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2064 3860 5572
Size: 274 80 33 51 1443 87 1796 1712
Cuts at: 5572 96
Size: 138
Fragments arranged by size:
1796 1712 1443 274 138 87 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5574 5606 5574
Size: 32 5582
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2712 2712
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 2721 1943
Size: 778 4836
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 2559 3743 2559
Size: 1184 4430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2233 3422 276
Size: 53 83 186 936 699 1189 2468
Fragments arranged by size:
2468 1189 936 699 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI