Restriction map for
pFN21AB7612
(Circular) (Six-base) MAPSORT of: pFN21AB7612.seq Check: 3328 from: 1 to: 5782
pFN21AB7612 5782 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2275 2789 3845 5229 5497 1632
Size: 643 514 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1056 643 514 268
AfeI AGC'GCT
Cuts at: 2732 2732
Size: 5782
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 2521 4511 2521
Size: 1990 3792
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2188 2377 2812 3525 5097 2188
Size: 189 435 713 1572 2873
Fragments arranged by size:
2873 1572 713 435 189
ApaLI G'TGCA_C
Cuts at: 2200 2555 3839 4995 2200
Size: 355 1284 1156 2987
Fragments arranged by size:
2987 1284 1156 355
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2141 2444 3107 1870
Size: 73 126 72 303 663 4545
Fragments arranged by size:
4545 663 303 126 73 72
AvrII C'CTAG_G
Cuts at: 2992 2992
Size: 5782
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2492 3061 3103 4558 618
Size: 325 217 627 705 569 42 1455 1842
Fragments arranged by size:
1842 1455 705 627 569 325 217 42
BanII G_rGCy'C
Cuts at: 1391 2699 3101 1391
Size: 1308 402 4072
Fragments arranged by size:
4072 1308 402
BbeI G_GCGC'C
Cuts at: 2496 2496
Size: 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 2579 928
Size: 971 332 348 4131
Fragments arranged by size:
4131 971 348 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2191 2225 2566 2600 3973 4007 1903
Size: 34 254 34 341 34 1373 34 3678
Fragments arranged by size:
3678 1373 341 254 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2050 1548
Size: 502 5280
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2286 4393 136
Size: 107 122 71 631 605 614 2107 1525
Fragments arranged by size:
2107 1525 631 614 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 2915 3199 2915
Size: 284 5498
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2559 3843 4999 1790
Size: 414 355 1284 1156 2573
Fragments arranged by size:
2573 1284 1156 414 355
BmgBI CAC'GTC
Cuts at: 1318 2199 3016 1318
Size: 881 817 4084
Fragments arranged by size:
4084 881 817
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2826 3513 4471
Size: 416 53 83 399 24 1409 687 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1409 958 687 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3004 3004
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 4442 1239
Size: 1676 1527 2579
Fragments arranged by size:
2579 1676 1527
Bpu10I CC'TnA_GC
Cuts at: 2693 2693
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 3907 4772 5070 2147
Size: 1760 865 298 2859
Fragments arranged by size:
2859 1760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2974 4445 882
Size: 617 1475 1471 2219
Fragments arranged by size:
2219 1475 1471 617
BsaAI yAC'GTr
Cuts at: 493 1204 2205 2554 3573 493
Size: 711 1001 349 1019 2702
Fragments arranged by size:
2702 1019 1001 711 349
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 2822 3589 3971
Size: 53 83 186 936 960 329 767 382
Cuts at: 3971 4606 275
Size: 635 1451
Fragments arranged by size:
1451 960 936 767 635 382 329 186
83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3005 3035 1178
Size: 30 165 30 1602 30 3925
Fragments arranged by size:
3925 1602 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2992 1186
Size: 1806 3976
BseYI C'CCAG_C
Cuts at: 2519 4985 5481 2519
Size: 2466 496 2820
Fragments arranged by size:
2820 2466 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2197 2383 2597 2885 2906 1163
Size: 573 461 186 214 288 21 4039
Fragments arranged by size:
4039 573 461 288 214 186 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2460 3232 3993 5021 1540
Size: 275 186 459 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 459 275 186
BsiHKAI G_wGCw'C
Cuts at: 2204 2409 2559 3843 3928 4999 2204
Size: 205 150 1284 85 1071 2987
Fragments arranged by size:
2987 1284 1071 205 150 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
BsmBI CGTCTCn'nnnn_
Cuts at: 2189 2211 3071 2189
Size: 22 860 4900
Fragments arranged by size:
4900 860 22
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2409 2559 2699 3101 3843 3928
Size: 399 414 205 150 140 402 742 85
Cuts at: 3928 4999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 414 402 399 205 150
140 85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2661 2760 3671 5401 2661
Size: 99 911 1730 3042
Fragments arranged by size:
3042 1730 911 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 3137 844
Size: 1792 501 3489
Fragments arranged by size:
3489 1792 501
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2459 2529 2687 4426 4613 1700
Size: 110 649 70 158 1739 187 2869
Fragments arranged by size:
2869 1739 649 187 158 110 70
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 2449 1925
Size: 524 5258
BssSI C'ACGA_G
Cuts at: 2408 3842 4854 2408
Size: 1434 1012 3336
Fragments arranged by size:
3336 1434 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2268 2413 2620 794
Size: 1474 145 207 3956
Fragments arranged by size:
3956 1474 207 145
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 3058 2086
Size: 972 4810
BstYI r'GATC_y
Cuts at: 1972 2103 3112 3479 3864 3881 5333 5419 5431
Size: 131 1009 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1009 385 367 346 131 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2431 2454 2497 2645 2699 2903
Size: 552 939 427 23 43 148 54 204
Cuts at: 2903 3072 513
Size: 169 3223
Fragments arranged by size:
3223 939 552 427 204 169 148 54
43 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 2883 3298 4092 4112 2883
Size: 415 794 20 4553
Fragments arranged by size:
4553 794 415 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3025 4789 809
Size: 507 1709 1764 1802
Fragments arranged by size:
1802 1764 1709 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2457 3229
Size: 54 1425 50 275 18 246 381 772
Cuts at: 3229 4118 5747 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 772 381 275 246 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2457 3229 1537
Size: 275 645 772 4090
Fragments arranged by size:
4090 772 645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 1353
Size: 2359 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2837 4369 4743 4889 387
Size: 1064 138 1248 1532 374 146 1280
Fragments arranged by size:
1532 1280 1248 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2275 2789 2915 3845 4442 5229 5497
Size: 393 643 514 126 930 597 787 268
Cuts at: 5497 1239
Size: 1524
Fragments arranged by size:
1524 930 787 643 597 514 393 268
126
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2496 2734 2915 4929 1678
Size: 818 238 181 2014 2531
Fragments arranged by size:
2531 2014 818 238 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 2507 2539 3005
Size: 32 135 32 806 32 97 32 466
Cuts at: 3005 3037 4423 4455 4497 4529 1373
Size: 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 806 466 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KasI G'GCGC_C
Cuts at: 2492 2492
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2983 4896 5080 1305
Size: 1678 1913 184 2007
Fragments arranged by size:
2007 1913 1678 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2759 3740 4099 4258 518
Size: 648 918 675 981 359 159 2042
Fragments arranged by size:
2042 981 918 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2352 2374 2456 2499 2611 2647 2674 2701
Size: 746 22 82 43 112 36 27 27
Cuts at: 2701 2770 2965 3198 3875 5023 5268 1606
Size: 69 195 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 746 677 245 233 195 112
82 69 43 36 27 27 22
NaeI GCC'GGC
Cuts at: 2461 2531 2461
Size: 70 5712
NarI GG'CG_CC
Cuts at: 2493 2493
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4291
Fragments arranged by size:
4291 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NgoMIV G'CCGG_C
Cuts at: 2459 2529 2459
Size: 70 5712
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 2965 1108
Size: 1080 777 3925
Fragments arranged by size:
3925 1080 777
PfoI T'CCnGG_A
Cuts at: 3039 3039
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2205 2554 2205
Size: 349 5433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PspXI vC'TCGA_Gb
Cuts at: 2141 2444 2141
Size: 303 5479
PstI C_TGCA'G
Cuts at: 830 2775 3134 830
Size: 1945 359 3478
Fragments arranged by size:
3478 1945 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2374 2611 2674 2770 2965 2374
Size: 237 63 96 195 5191
Fragments arranged by size:
5191 237 195 96 63
SacII CC_GC'GG
Cuts at: 2457 2500 2648 2702 2457
Size: 43 148 54 5537
Fragments arranged by size:
5537 148 54 43
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SexAI A'CCwGG_T
Cuts at: 2304 2304
Size: 5782
SfcI C'TryA_G
Cuts at: 826 1045 2771 3130 4265 4946 5137 826
Size: 219 1726 359 1135 681 191 1471
Fragments arranged by size:
1726 1471 1135 681 359 219 191
SfoI GGC'GCC
Cuts at: 2494 2494
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2071 3109 1872
Size: 199 1038 4545
Fragments arranged by size:
4545 1038 199
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 2162 2444 3886 4787 5049
Size: 197 926 198 21 282 1442 901 262
Cuts at: 5049 820
Size: 1553
Fragments arranged by size:
1553 1442 926 901 282 262 198 197
21
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2992 3221 513
Size: 552 939 988 229 3074
Fragments arranged by size:
3074 988 939 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2036 3809 3826 3979 4164 4609
Size: 1255 447 9 1773 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1773 1498 1255 447 445 185 153 17
9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4028 5740 96
Size: 274 80 33 51 1443 2051 1712 138
Fragments arranged by size:
2051 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 5742 5774 2183
Size: 32 3527 32 2191
Fragments arranged by size:
3527 2191 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2246 1316
Size: 930 4852
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2378 2281
Size: 97 5685
XhoI C'TCGA_G
Cuts at: 1943 2141 2444 1943
Size: 198 303 5281
Fragments arranged by size:
5281 303 198
XmaI C'CCGG_G
Cuts at: 1870 2069 3107 1870
Size: 199 1038 4545
Fragments arranged by size:
4545 1038 199
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI NruI NsiI PacI PpuMI PspOMI PsrI
RsrII SacI SanDI SapI SfiI SrfI SwaI