Restriction map for
pFN21AB7611
(Circular) (Six-base) MAPSORT of: pFN21AB7611.seq Check: 3094 from: 1 to: 5611
pFN21AB7611 5611 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2818 3674 5058 5326 1632
Size: 1186 856 1384 268 1917
Fragments arranged by size:
1917 1384 1186 856 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 4510 5487 1203
Size: 3307 977 1327
Fragments arranged by size:
3307 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2066 2639 2656 3354 4926 2066
Size: 573 17 698 1572 2751
Fragments arranged by size:
2751 1572 698 573 17
ApaI G_GGCC'C
Cuts at: 2647 2669 2647
Size: 22 5589
ApaLI G'TGCA_C
Cuts at: 2849 3668 4824 2849
Size: 819 1156 3636
Fragments arranged by size:
3636 1156 819
ApoI r'AATT_y
Cuts at: 1493 2920 3146 3298 5407 1493
Size: 1427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 226 152
AscI GG'CGCG_CC
Cuts at: 2344 2344
Size: 5611
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 2620 2672 2788 2936 1870
Size: 73 471 206 52 116 148 4545
Fragments arranged by size:
4545 471 206 148 116 73 52
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 2425 2599 2624 2932
Size: 325 217 627 620 18 174 25 308
Cuts at: 2932 4387 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 620 325 308 217 174
25 18
BanII G_rGCy'C
Cuts at: 1391 2045 2647 2669 2930 1391
Size: 654 602 22 261 4072
Fragments arranged by size:
4072 654 602 261 22
BbeI G_GCGC'C
Cuts at: 2411 2411
Size: 5611
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2557 928
Size: 971 658 3982
Fragments arranged by size:
3982 971 658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2270 2304 3802 3836 1903
Size: 34 333 34 1498 34 3678
Fragments arranged by size:
3678 1498 333 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2744 4222 136
Size: 107 122 71 631 605 1072 1478 1525
Fragments arranged by size:
1525 1478 1072 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 2021 3028 2021
Size: 1007 4604
Bme1580I G_kGCm'C
Cuts at: 1790 2604 2627 2647 2669 2853 3672 4828 1790
Size: 814 23 20 22 184 819 1156 2573
Fragments arranged by size:
2573 1156 819 814 184 23 22 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3342 4300 442
Size: 416 53 83 399 24 1925 958 1753
Fragments arranged by size:
1925 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4271 1239
Size: 3032 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4601 4899 3736
Size: 865 298 4448
Fragments arranged by size:
4448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 882
Size: 617 2775 2219
Fragments arranged by size:
2775 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2408 3418 3800 4435
Size: 53 83 186 936 875 1010 382 635
Cuts at: 4435 275
Size: 1451
Fragments arranged by size:
1451 1010 936 875 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5386
Fragments arranged by size:
5386 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2453 1186
Size: 1111 156 4344
Fragments arranged by size:
4344 1111 156
BseYI C'CCAG_C
Cuts at: 2082 2576 4814 5310 2082
Size: 494 2238 496 2383
Fragments arranged by size:
2383 2238 496 494
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5038
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2688 2730 3061 3822 4850 1540
Size: 275 186 687 42 331 761 1028 2301
Fragments arranged by size:
2301 1028 761 687 331 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2097 2118 2276 2853 3672 3757 4828 2097
Size: 21 158 577 819 85 1071 2880
Fragments arranged by size:
2880 1071 819 577 158 85 21
BsiWI C'GTAC_G
Cuts at: 2460 2460
Size: 5611
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2097 2118 2276 2604 2627 2647
Size: 399 255 52 21 158 328 23 20
Cuts at: 2647 2669 2853 2930 3672 3757 4828 1391
Size: 22 184 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 255 184 158
85 77 52 23 22 21 20
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2966 844
Size: 2122 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2413 2497 3498 1394
Size: 429 590 84 1001 3507
Fragments arranged by size:
3507 1001 590 429 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2340 4255 4442 1700
Size: 110 530 1915 187 2869
Fragments arranged by size:
2869 1915 530 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 2344 1925
Size: 419 5192
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5611
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606
Size: 969 367 385 17 1452 86 12 346
Cuts at: 5606 1972
Size: 1977
Fragments arranged by size:
1977 1452 969 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2595 2610 2742 2895 513
Size: 552 939 591 15 132 153 3229
Fragments arranged by size:
3229 939 591 552 153 132 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2569 487
Size: 683 56 1343 3529
Fragments arranged by size:
3529 1343 683 56
BtsI GCAGTG_nn'
Cuts at: 3127 3921 3941 3127
Size: 794 20 4797
Fragments arranged by size:
4797 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2471 2685 2727
Size: 54 1425 50 275 18 641 214 42
Cuts at: 2727 2796 3058 3947 5576 8
Size: 69 262 889 1629 43
Fragments arranged by size:
1629 1425 889 641 275 262 214 69
54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2685 2727 3058 1537
Size: 275 873 42 331 4090
Fragments arranged by size:
4090 873 331 275 42
EarI CTCTTCn'nnn_
Cuts at: 1353 2761 3541 1353
Size: 1408 780 3423
Fragments arranged by size:
3423 1408 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2371 2596 4198 4572 4718 387
Size: 1064 138 782 225 1602 374 146 1280
Fragments arranged by size:
1602 1280 1064 782 374 225 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2818 3674 4271 5058 5326 1239
Size: 393 1186 856 597 787 268 1524
Fragments arranged by size:
1524 1186 856 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2248 2309 2248
Size: 61 5550
EcoO109I rG'GnC_Cy
Cuts at: 2543 2636 2643 2644 2653 2665 2666 2776 2543
Size: 93 7 1 9 12 1 110 5378
Fragments arranged by size:
5378 110 93 12 9 7 1 1
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 2411 4758 1678
Size: 733 2347 2531
Fragments arranged by size:
2531 2347 733
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373
Size: 32 135 32 2680 32 42 32 2626
Fragments arranged by size:
2680 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2536 2955 3201 669
Size: 988 281 598 419 246 3079
Fragments arranged by size:
3079 988 598 419 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KasI G'GCGC_C
Cuts at: 2407 2407
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4725 4909 1305
Size: 3420 184 2007
Fragments arranged by size:
3420 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2798 10
Size: 54 1425 343 966 2823
Fragments arranged by size:
2823 1425 966 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3569 3928 4087 518
Size: 648 2403 359 159 2042
Fragments arranged by size:
2403 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2612 2744 2846 3027 3704 4852 5097
Size: 585 421 132 102 181 677 1148 245
Cuts at: 5097 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 585 421 245 181 132
102
NarI GG'CG_CC
Cuts at: 2408 2408
Size: 5611
NcoI C'CATG_G
Cuts at: 513 1065 2004 2595 513
Size: 552 939 591 3529
Fragments arranged by size:
3529 939 591 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2969 4514 1132
Size: 97 1740 1545 2229
Fragments arranged by size:
2229 1740 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2639 1108
Size: 1531 4080
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2776 2776
Size: 5611
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PspOMI G'GGCC_C
Cuts at: 2643 2665 2643
Size: 22 5589
PspXI vC'TCGA_Gb
Cuts at: 2788 2788
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2652 2963 830
Size: 1822 311 3478
Fragments arranged by size:
3478 1822 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
SacII CC_GC'GG
Cuts at: 2613 2745 2613
Size: 132 5479
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SanDI GG'GwC_CC
Cuts at: 2776 2776
Size: 5611
SbfI CC_TGCA'GG
Cuts at: 2652 2963 2652
Size: 311 5300
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2225 2648 2959 4094 4775 4966 826
Size: 219 1180 423 311 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 681 423 311 219 191
SfoI GGC'GCC
Cuts at: 2409 2409
Size: 5611
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2622 2674 2938 1872
Size: 750 52 264 4545
Fragments arranged by size:
4545 750 264 52
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2788 3715 4616 4878 820
Size: 197 926 845 927 901 262 1553
Fragments arranged by size:
1553 927 926 901 845 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 2135 1517
Size: 618 4993
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 2595 3050 513
Size: 552 939 474 117 455 3074
Fragments arranged by size:
3074 939 552 474 455 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2521 2590 3638 3655 3808 3993 4438
Size: 1255 941 69 1048 17 153 185 445
Cuts at: 4438 325
Size: 1498
Fragments arranged by size:
1498 1255 1048 941 445 185 153 69
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3857 5569 96
Size: 274 80 33 51 1443 1880 1712 138
Fragments arranged by size:
1880 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5611
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 2788 1943
Size: 845 4766
XmaI C'CCGG_G
Cuts at: 1870 2620 2672 2936 1870
Size: 750 52 264 4545
Fragments arranged by size:
4545 750 264 52
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PsrI PvuII RsrII SacI SapI SexAI
SfiI SrfI SwaI XcmI