Restriction map for
pFN21AB7608
(Circular) (Six-base) MAPSORT of: pFN21AB7608.seq Check: 1049 from: 1 to: 5776
pFN21AB7608 5776 bp
With 182 enzymes: SgfI *
December 17, 2010 17:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2150 2150
Size: 5776
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3012 3097 3012
Size: 85 5691
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2092 3839 5223 5491 1632
Size: 460 1747 1384 268 1917
Fragments arranged by size:
1917 1747 1384 460 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 3037 3519 5091 3037
Size: 482 1572 3722
Fragments arranged by size:
3722 1572 482
ApaI G_GGCC'C
Cuts at: 2365 2365
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2997 4330 160
Size: 872 1965 1333 1606
Fragments arranged by size:
1965 1606 1333 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2940 3101 1870
Size: 73 997 161 4545
Fragments arranged by size:
4545 997 161 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 2381 3012 3097 4552
Size: 325 217 627 397 197 631 85 1455
Cuts at: 4552 618
Size: 1842
Fragments arranged by size:
1842 1455 631 627 397 325 217 197
85
BanII G_rGCy'C
Cuts at: 1391 2365 2695 2988 3095 1391
Size: 974 330 293 107 4072
Fragments arranged by size:
4072 974 330 293 107
BbeI G_GCGC'C
Cuts at: 2188 2188
Size: 5776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2539 2574 928
Size: 971 640 35 4130
Fragments arranged by size:
4130 971 640 35
BbvCI CC'TCA_GC
Cuts at: 2027 2027
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 2875 1548
Size: 1327 4449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2379 4387 136
Size: 107 122 71 631 605 707 2008 1525
Fragments arranged by size:
2008 1525 707 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2574 3066 5771 2574
Size: 492 2705 2579
Fragments arranged by size:
2705 2579 492
BlpI GC'TnA_GC
Cuts at: 2982 3193 2982
Size: 211 5565
Bme1580I G_kGCm'C
Cuts at: 1790 2302 2365 2384 3837 4993 1790
Size: 512 63 19 1453 1156 2573
Fragments arranged by size:
2573 1453 1156 512 63 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 2871 3044 4436 1239
Size: 1161 471 173 1392 2579
Fragments arranged by size:
2579 1392 1161 471 173
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 3901 4766 5064 2570
Size: 1331 865 298 3282
Fragments arranged by size:
3282 1331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2185 3583 3965 4600
Size: 53 83 186 936 652 1398 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1451 1398 936 652 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2346 2988 4209 4607 4881 5028 5439 2346
Size: 642 1221 398 274 147 411 2683
Fragments arranged by size:
2683 1221 642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2561 2583 2591 2613 2682
Size: 30 165 30 1158 22 8 22 69
Cuts at: 2682 2712 2760 2790 1178
Size: 30 48 30 4164
Fragments arranged by size:
4164 1158 165 69 48 30 30 30
30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2903 1186
Size: 1717 4059
BseYI C'CCAG_C
Cuts at: 2194 2289 4979 5475 2194
Size: 95 2690 496 2495
Fragments arranged by size:
2690 2495 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2423 1163
Size: 573 687 4516
Fragments arranged by size:
4516 687 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2799 2988 3837 3922 4993 2799
Size: 189 849 85 1071 3582
Fragments arranged by size:
3582 1071 849 189 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2302 2365 2384 2695 2799 2988 3095
Size: 399 512 63 19 311 104 189 107
Cuts at: 3095 3837 3922 4993 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 512 399 311 189 107
104 85 63 19
BspEI T'CCGG_A
Cuts at: 2346 2988 5439 2346
Size: 642 2451 2683
Fragments arranged by size:
2683 2451 642
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2150 2193 2547 3131 844
Size: 1306 43 354 584 3489
Fragments arranged by size:
3489 1306 584 354 43
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2267 4271 4445 65
Size: 1183 1019 2004 174 1396
Fragments arranged by size:
2004 1396 1183 1019 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2574 3066 3106 3473 3858 3875 5327 5413
Size: 602 492 40 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 602 492 385 367 346 86
40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2752 2808 513
Size: 552 939 748 56 3481
Fragments arranged by size:
3481 939 748 552 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 3292 4086 4106 2188
Size: 1104 794 20 3858
Fragments arranged by size:
3858 1104 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2543 4783 809
Size: 507 1227 2240 1802
Fragments arranged by size:
2240 1802 1227 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3223 4112
Size: 54 1425 50 275 18 177 1216 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1216 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 2673 2822 3706 1353
Size: 978 342 149 884 3423
Fragments arranged by size:
3423 978 884 342 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2986 2986
Size: 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2400 2871 3044 3839 4436 5223
Size: 393 460 308 471 173 795 597 787
Cuts at: 5223 5491 1239
Size: 268 1524
Fragments arranged by size:
1524 795 787 597 471 460 393 308
268 173
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 2585
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2361 2536 2937 3034 2361
Size: 175 401 97 5103
Fragments arranged by size:
5103 401 175 97
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2188 4923 1678
Size: 510 2735 2531
Fragments arranged by size:
2735 2531 510
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 4417 4449 4491
Size: 32 135 32 989 32 1824 32 42
Cuts at: 4491 4523 1373
Size: 32 2626
Fragments arranged by size:
2626 1824 989 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2184 2184
Size: 5776
KpnI G_GTAC'C
Cuts at: 3016 3101 3016
Size: 85 5691
MfeI C'AATT_G
Cuts at: 2679 3375 2679
Size: 696 5080
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3777
Fragments arranged by size:
3777 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2504 2751 3734 4093 4252 518
Size: 648 907 431 247 983 359 159 2042
Fragments arranged by size:
2042 983 907 648 431 359 247 159
MspA1I CmG'CkG
Cuts at: 1606 2293 2597 2881 3055 3192 3869 5017 5262
Size: 687 304 284 174 137 677 1148 245
Cuts at: 5262 1606
Size: 2120
Fragments arranged by size:
2120 1148 687 677 304 284 245 174
137
NarI GG'CG_CC
Cuts at: 2185 2185
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 2004 2752 2808 513
Size: 552 939 748 56 3481
Fragments arranged by size:
3481 939 748 552 56
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 3060 3060
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2536 2536
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2361 2361
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2204 2775 3074 3128 830
Size: 1374 571 299 54 3478
Fragments arranged by size:
3478 1374 571 299 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2597 2881 2597
Size: 284 5492
SacI G_AGCT'C
Cuts at: 2988 2988
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2331 2331
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 2204 3128 2204
Size: 924 4852
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2200 2771 3070 3124 4259 4940 5131
Size: 219 1155 571 299 54 1135 681 191
Cuts at: 5131 826
Size: 1471
Fragments arranged by size:
1471 1155 1135 681 571 299 219 191
54
SfoI GGC'GCC
Cuts at: 2186 2186
Size: 5776
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 2942 3103 1872
Size: 1070 161 4545
Fragments arranged by size:
4545 1070 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 3880 4781 5043 820
Size: 197 926 642 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 642 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2413 2973 3005 1517
Size: 896 560 32 4288
Fragments arranged by size:
4288 896 560 32
StyI C'CwwG_G
Cuts at: 513 1065 2004 2752 2808 3215 513
Size: 552 939 748 56 407 3074
Fragments arranged by size:
3074 939 748 552 407 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2859 3803 3820 3973 4158 4603 325
Size: 1255 1279 944 17 153 185 445 1498
Fragments arranged by size:
1498 1279 1255 944 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 2637 2669 5736 5768 2583
Size: 32 22 32 3067 32 2591
Fragments arranged by size:
3067 2591 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 2940 3101 1870
Size: 1070 161 4545
Fragments arranged by size:
4545 1070 161
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI