Restriction map for
pFN21AB7604
(Circular) (Six-base) MAPSORT of: pFN21AB7604.seq Check: 1232 from: 1 to: 5770
pFN21AB7604 5770 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2831 2831
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2403 2891 3833 5217 5485 1632
Size: 771 488 942 1384 268 1917
Fragments arranged by size:
1917 1384 942 771 488 268
AfeI AGC'GCT
Cuts at: 2081 2081
Size: 5770
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 2895 1166
Size: 1729 4041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaI G_GGCC'C
Cuts at: 2322 2468 2625 2322
Size: 146 157 5467
Fragments arranged by size:
5467 157 146
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AscI GG'CGCG_CC
Cuts at: 2241 2613 2241
Size: 372 5398
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2297 2417 2461 2492 2618 3095
Size: 73 156 198 120 44 31 126 477
Cuts at: 3095 1870
Size: 4545
Fragments arranged by size:
4545 477 198 156 126 120 73 44
31
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2174 2347 2456 2707
Size: 325 217 627 324 63 173 109 251
Cuts at: 2707 3091 4546 618
Size: 384 1455 1842
Fragments arranged by size:
1842 1455 627 384 325 324 251 217
173 109 63
BanII G_rGCy'C
Cuts at: 1391 2322 2379 2468 2625 2961 3089 1391
Size: 931 57 89 157 336 128 4072
Fragments arranged by size:
4072 931 336 157 128 89 57
BbeI G_GCGC'C
Cuts at: 2178 2351 2460 2711 2178
Size: 173 109 251 5237
Fragments arranged by size:
5237 251 173 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 3961 3995 1903
Size: 34 351 34 1639 34 3678
Fragments arranged by size:
3678 1639 351 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2317 2541 2690
Size: 107 122 71 631 605 645 224 149
Cuts at: 2690 4381 136
Size: 1691 1525
Fragments arranged by size:
1691 1525 645 631 605 224 149 122
107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 2531 3187 2531
Size: 656 5114
Bme1580I G_kGCm'C
Cuts at: 1790 2322 2468 2625 3831 4987 1790
Size: 532 146 157 1206 1156 2573
Fragments arranged by size:
2573 1206 1156 532 157 146
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2929 3501 4459
Size: 416 53 83 399 24 1512 572 958
Cuts at: 4459 442
Size: 1753
Fragments arranged by size:
1753 1512 958 572 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2774 2783 4430 1239
Size: 1535 9 1647 2579
Fragments arranged by size:
2579 1647 1535 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2429 3895 4760 5058 2429
Size: 1466 865 298 3141
Fragments arranged by size:
3141 1466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 2732 4433 882
Size: 617 1170 63 1701 2219
Fragments arranged by size:
2219 1701 1170 617 63
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 2348 2457 2708
Size: 53 83 186 936 642 173 109 251
Cuts at: 2708 3577 3959 4594 275
Size: 869 382 635 1451
Fragments arranged by size:
1451 936 869 642 635 382 251 186
173 109 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 1186
Size: 1534 4236
BseYI C'CCAG_C
Cuts at: 2653 4973 5469 2653
Size: 2320 496 2954
Fragments arranged by size:
2954 2320 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 3035 1163
Size: 573 576 723 3898
Fragments arranged by size:
3898 723 576 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2335 2359 2682 2785 3019 3220
Size: 275 186 334 24 323 103 234 201
Cuts at: 3220 3981 5009 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 334 323 275 234 201
186 103 24
BsiHKAI G_wGCw'C
Cuts at: 2961 3831 3916 4987 2961
Size: 870 85 1071 3744
Fragments arranged by size:
3744 1071 870 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2379 2468 2625 2961 3089 3831
Size: 399 532 57 89 157 336 128 742
Cuts at: 3831 3916 4987 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 399 336 157 128
89 85 57
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2831 3125 844
Size: 1987 294 3489
Fragments arranged by size:
3489 1987 294
BsrBI CCG'CTC
Cuts at: 1394 1823 2530 2790 3657 1394
Size: 429 707 260 867 3507
Fragments arranged by size:
3507 867 707 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2991 4414 4601 1700
Size: 110 1181 1423 187 2869
Fragments arranged by size:
2869 1423 1181 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 2089 2241 2549 2572 2613 2945 1925
Size: 164 152 308 23 41 332 4750
Fragments arranged by size:
4750 332 308 164 152 41 23
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2161
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765
Size: 1128 367 385 17 1452 86 12 346
Cuts at: 5765 1972
Size: 1977
Fragments arranged by size:
1977 1452 1128 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2265 2428 2688 2722 513
Size: 552 939 150 111 163 260 34 3561
Fragments arranged by size:
3561 939 552 260 163 150 111 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 2324 2911 3286 4080 4100 2324
Size: 587 375 794 20 3994
Fragments arranged by size:
3994 794 587 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2084 2332 2356
Size: 54 1425 50 275 18 254 248 24
Cuts at: 2356 2679 2782 2993 3217 4106 5735 8
Size: 323 103 211 224 889 1629 43
Fragments arranged by size:
1629 1425 889 323 275 254 248 224
211 103 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2332 2356 2679 2782 3217 1537
Size: 275 520 24 323 103 435 4090
Fragments arranged by size:
4090 520 435 323 275 103 24
EarI CTCTTCn'nnn_
Cuts at: 1353 2672 3700 1353
Size: 1319 1028 3423
Fragments arranged by size:
3423 1319 1028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2959 2959
Size: 5770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2774 2783 2891 3833 4430 5217
Size: 393 771 371 9 108 942 597 787
Cuts at: 5217 5485 1239
Size: 268 1524
Fragments arranged by size:
1524 942 787 771 597 393 371 268
108 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2366
Size: 5770
EcoO109I rG'GnC_Cy
Cuts at: 2318 2489 2318
Size: 171 5599
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FspI TGC'GCA
Cuts at: 2885 4276 2885
Size: 1391 4379
HaeII r_GCGC'y
Cuts at: 1678 2012 2083 2178 2262 2351 2460 2711 3050
Size: 334 71 95 84 89 109 251 339
Cuts at: 3050 4917 1678
Size: 1867 2531
Fragments arranged by size:
2531 1867 339 334 251 109 95 89
84 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2851 2883 4411 4443 4485
Size: 32 135 32 1279 32 1528 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 1528 1279 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KasI G'GCGC_C
Cuts at: 2174 2347 2456 2707 2174
Size: 173 109 251 5237
Fragments arranged by size:
5237 251 173 109
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2895 3001 3728 4087 4246 518
Size: 648 1729 106 727 359 159 2042
Fragments arranged by size:
2042 1729 727 648 359 159 106
MspA1I CmG'CkG
Cuts at: 1606 2267 2690 2724 2752 3186 3863 5011 5256
Size: 661 423 34 28 434 677 1148 245
Cuts at: 5256 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 661 434 423 245 34
28
NarI GG'CG_CC
Cuts at: 2175 2348 2457 2708 2175
Size: 173 109 251 5237
Fragments arranged by size:
5237 251 173 109
NcoI C'CATG_G
Cuts at: 513 1065 2004 2154 513
Size: 552 939 150 4129
Fragments arranged by size:
4129 939 552 150
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 2679 2782 3217 2679
Size: 103 435 5232
Fragments arranged by size:
5232 435 103
NspI r_CATG'y
Cuts at: 1132 1229 2485 3128 4673 1132
Size: 97 1256 643 1545 2229
Fragments arranged by size:
2229 1545 1256 643 97
PasI CC'CwG_GG
Cuts at: 1348 2698 1348
Size: 1350 4420
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2489 2489
Size: 5770
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PspOMI G'GGCC_C
Cuts at: 2318 2464 2621 2318
Size: 146 157 5467
Fragments arranged by size:
5467 157 146
PstI C_TGCA'G
Cuts at: 830 2076 2828 3122 830
Size: 1246 752 294 3478
Fragments arranged by size:
3478 1246 752 294
PvuI CG_AT'CG
Cuts at: 2001 3019 2001
Size: 1018 4752
RsrII CG'GwC_CG
Cuts at: 2567 2567
Size: 5770
SacI G_AGCT'C
Cuts at: 2961 2961
Size: 5770
SacII CC_GC'GG
Cuts at: 2268 2691 2725 2268
Size: 423 34 5313
Fragments arranged by size:
5313 423 34
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SbfI CC_TGCA'GG
Cuts at: 2076 3122 2076
Size: 1046 4724
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 2072 2254 2824 3118 4253 4934 5125
Size: 219 1027 182 570 294 1135 681 191
Cuts at: 5125 826
Size: 1471
Fragments arranged by size:
1471 1135 1027 681 570 294 219 191
182
SfoI GGC'GCC
Cuts at: 2176 2349 2458 2709 2176
Size: 173 109 251 5237
Fragments arranged by size:
5237 251 173 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 2419 2463 2494 2620 3097 1872
Size: 547 44 31 126 477 4545
Fragments arranged by size:
4545 547 477 126 44 31
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 3874 4775 5037 820
Size: 197 926 501 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 501 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 2485 3128 1132
Size: 1353 643 3774
Fragments arranged by size:
3774 1353 643
SrfI GCCC'GGGC
Cuts at: 2620 2620
Size: 5770
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 3209 513
Size: 552 939 150 1055 3074
Fragments arranged by size:
3074 1055 939 552 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 3797 3814 3967 4152 4597 325
Size: 1255 1071 1146 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1146 1071 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4016 5728 96
Size: 274 80 33 51 1443 2039 1712 138
Fragments arranged by size:
2039 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 2417 2461 2492 2618 3095 1870
Size: 547 44 31 126 477 4545
Fragments arranged by size:
4545 547 477 126 44 31
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
RsrII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII SanDI SapI SexAI SfiI SwaI XcmI