Restriction map for
pFN21AB7595
(Circular) (Six-base) MAPSORT of: pFN21AB7595.seq Check: 3547 from: 1 to: 5404
pFN21AB7595 5404 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2682 2747 1937
Size: 745 65 4594
Fragments arranged by size:
4594 745 65
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3467 4851 5119 1632
Size: 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 268
AfeI AGC'GCT
Cuts at: 2632 2632
Size: 5404
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 4303 5280 1203
Size: 3100 977 1327
Fragments arranged by size:
3100 1327 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 2450 1166
Size: 1284 4120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5404
AlwNI CAG_nnn'CTG
Cuts at: 2317 3147 4719 2317
Size: 830 1572 3002
Fragments arranged by size:
3002 1572 830
ApaI G_GGCC'C
Cuts at: 2076 2076
Size: 5404
ApaLI G'TGCA_C
Cuts at: 3461 4617 3461
Size: 1156 4248
ApoI r'AATT_y
Cuts at: 1493 2713 2939 3091 5200 1493
Size: 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 2729 1870
Size: 73 311 475 4545
Fragments arranged by size:
4545 475 311 73
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2380 2725 4180 618
Size: 325 217 627 345 248 345 1455 1842
Fragments arranged by size:
1842 1455 627 345 345 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2076 2084 2531 2583 2723 1391
Size: 685 8 447 52 140 4072
Fragments arranged by size:
4072 685 447 140 52 8
BbeI G_GCGC'C
Cuts at: 2136 2136
Size: 5404
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2069 2593 928
Size: 971 170 524 3739
Fragments arranged by size:
3739 971 524 170
BbvCI CC'TCA_GC
Cuts at: 2102 2298 2559 2102
Size: 196 261 4947
Fragments arranged by size:
4947 261 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2342 4015
Size: 107 122 71 631 605 376 294 1673
Cuts at: 4015 136
Size: 1525
Fragments arranged by size:
1673 1525 631 605 376 294 122 107
71
BglII A'GATC_T
Cuts at: 5399 5399
Size: 5404
BlpI GC'TnA_GC
Cuts at: 2821 2821
Size: 5404
Bme1580I G_kGCm'C
Cuts at: 1790 2076 3465 4621 1790
Size: 286 1389 1156 2573
Fragments arranged by size:
2573 1389 1156 286
BmgBI CAC'GTC
Cuts at: 1318 2032 1318
Size: 714 4690
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2294 2630 4064 1239
Size: 829 226 336 1434 2579
Fragments arranged by size:
2579 1434 829 336 226
Bpu10I CC'TnA_GC
Cuts at: 2102 2298 2559 2102
Size: 196 261 4947
Fragments arranged by size:
4947 261 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2277 3529 4394 4692 2277
Size: 1252 865 298 2989
Fragments arranged by size:
2989 1252 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2537 4067 882
Size: 617 1038 1530 2219
Fragments arranged by size:
2219 1530 1038 617
BsaAI yAC'GTr
Cuts at: 493 1204 3195 493
Size: 711 1991 2702
Fragments arranged by size:
2702 1991 711
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 3211 3593 4228
Size: 53 83 186 936 600 1078 382 635
Cuts at: 4228 275
Size: 1451
Fragments arranged by size:
1451 1078 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5179
Fragments arranged by size:
5179 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2447 2501 1186
Size: 874 387 54 4089
Fragments arranged by size:
4089 874 387 54
BseYI C'CCAG_C
Cuts at: 4607 5103 4607
Size: 496 4908
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 1163
Size: 573 405 4426
Fragments arranged by size:
4426 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3615 4643 1540
Size: 275 186 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2457 3465 3550 4621 2012
Size: 445 1008 85 1071 2795
Fragments arranged by size:
2795 1071 1008 445 85
BsmI GAATG_Cn'
Cuts at: 2915 3008 2915
Size: 93 5311
BsmBI CGTCTCn'nnnn_
Cuts at: 2491 2491
Size: 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2076 2084 2457 2531 2583 2723
Size: 399 222 64 8 373 74 52 140
Cuts at: 2723 3465 3550 4621 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 222 140 85
74 64 52 8
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2668 2759 844
Size: 1824 91 3489
Fragments arranged by size:
3489 1824 91
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2694 3899 4073 65
Size: 1183 1446 1205 174 1396
Fragments arranged by size:
1446 1396 1205 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4235 1700
Size: 110 2238 187 2869
Fragments arranged by size:
2869 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5404
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 3464 4476 3464
Size: 1012 4392
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5404
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 2618 1184
Size: 1434 3970
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 2590 2353
Size: 237 5167
BstYI r'GATC_y
Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399
Size: 762 367 385 17 1452 86 12 346
Cuts at: 5399 1972
Size: 1977
Fragments arranged by size:
1977 1452 762 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4852
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2464 487
Size: 683 56 1238 3427
Fragments arranged by size:
3427 1238 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3714 3734 2920
Size: 794 20 4590
Fragments arranged by size:
4590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2343 2851
Size: 54 1425 50 275 18 210 303 508
Cuts at: 2851 3740 5369 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 508 303 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2150 2181 2426 3334 1353
Size: 761 36 31 245 908 3423
Fragments arranged by size:
3423 908 761 245 36 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2294 2630 3467 4064 4851 5119
Size: 393 436 226 336 837 597 787 268
Cuts at: 5119 1239
Size: 1524
Fragments arranged by size:
1524 837 787 597 436 393 336 268
226
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 2536 2423
Size: 113 5291
EcoO109I rG'GnC_Cy
Cuts at: 2072 2073 2200 2392 2416 2072
Size: 1 127 192 24 5060
Fragments arranged by size:
5060 192 127 24 1
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 2054 2136 2634 4551 1678
Size: 376 82 498 1917 2531
Fragments arranged by size:
2531 1917 498 376 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4045 4077 4119 4151 1373
Size: 32 135 32 2473 32 42 32 2626
Fragments arranged by size:
2626 2473 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 2748 2994 669
Size: 988 281 568 242 246 3079
Fragments arranged by size:
3079 988 568 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KasI G'GCGC_C
Cuts at: 2132 2132
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2345 10
Size: 54 1425 343 513 3069
Fragments arranged by size:
3069 1425 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 3362 3721 3880 518
Size: 648 1284 912 359 159 2042
Fragments arranged by size:
2042 1284 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2374 2608 2820 3497 4645 4890 1606
Size: 768 234 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 768 677 245 234 212
NarI GG'CG_CC
Cuts at: 2133 2133
Size: 5404
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4852
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2762 4307 1132
Size: 97 1533 1545 2229
Fragments arranged by size:
2229 1545 1533 97
PasI CC'CwG_GG
Cuts at: 1348 2077 2196 1348
Size: 729 119 4556
Fragments arranged by size:
4556 729 119
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2571 1108
Size: 1269 194 3941
Fragments arranged by size:
3941 1269 194
PfoI T'CCnGG_A
Cuts at: 2025 2025
Size: 5404
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2416 2200
Size: 216 5188
PshAI GACnn'nnGTC
Cuts at: 1613 2501 1613
Size: 888 4516
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PspOMI G'GGCC_C
Cuts at: 2072 2072
Size: 5404
PstI C_TGCA'G
Cuts at: 830 2756 830
Size: 1926 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
PvuII CAG'CTG
Cuts at: 2374 2608 2374
Size: 234 5170
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SapI GCTCTTCn'nnn_
Cuts at: 2150 2150
Size: 5404
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2268 2752 3887 4568 4759 826
Size: 219 1223 484 1135 681 191 1471
Fragments arranged by size:
1471 1223 1135 681 484 219 191
SfoI GGC'GCC
Cuts at: 2134 2134
Size: 5404
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2731 1872
Size: 859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2292 3508 4409 4671 820
Size: 197 926 349 1216 901 262 1553
Fragments arranged by size:
1553 1216 926 901 349 262 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5404
StyI C'CwwG_G
Cuts at: 513 1065 2431 2499 2843 513
Size: 552 1366 68 344 3074
Fragments arranged by size:
3074 1366 552 344 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 2477 2637 3431 3448 3601 3786
Size: 1255 882 15 160 794 17 153 185
Cuts at: 3786 4231 325
Size: 445 1498
Fragments arranged by size:
1498 1255 882 794 445 185 160 153
17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3650 5362 96
Size: 274 80 33 51 1443 1673 1712 138
Fragments arranged by size:
1712 1673 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2338
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2729 1870
Size: 859 4545
XmnI GAAnn'nnTTC
Cuts at: 3533 3533
Size: 5404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI