Restriction map for
pFN21AB7594
(Circular) (Six-base) MAPSORT of: pFN21AB7594.seq Check: 186 from: 1 to: 5578
pFN21AB7594 5578 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3641 5025 5293 1632
Size: 2009 1384 268 1917
Fragments arranged by size:
2009 1917 1384 268
AfeI AGC'GCT
Cuts at: 2555 2555
Size: 5578
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AlwNI CAG_nnn'CTG
Cuts at: 2824 3321 4893 2824
Size: 497 1572 3509
Fragments arranged by size:
3509 1572 497
ApaI G_GGCC'C
Cuts at: 2117 2145 2117
Size: 28 5550
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AscI GG'CGCG_CC
Cuts at: 2205 2205
Size: 5578
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2294 2526 2903 1870
Size: 73 204 147 232 377 4545
Fragments arranged by size:
4545 377 232 204 147 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2123 2162 2272 2899
Size: 325 217 627 224 112 39 110 627
Cuts at: 2899 4354 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 627 325 224 217 112
110 39
BanII G_rGCy'C
Cuts at: 1391 2049 2117 2145 2897 1391
Size: 658 68 28 752 4072
Fragments arranged by size:
4072 752 658 68 28
BbeI G_GCGC'C
Cuts at: 2015 2127 2166 2015
Size: 112 39 5427
Fragments arranged by size:
5427 112 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2121 2160
Size: 107 122 71 631 605 337 112 39
Cuts at: 2160 2591 4189 136
Size: 431 1598 1525
Fragments arranged by size:
1598 1525 631 605 431 337 122 112
107 71 39
BglII A'GATC_T
Cuts at: 2535 5573 2535
Size: 3038 2540
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2145 2349 3639 4795 1790
Size: 327 28 204 1290 1156 2573
Fragments arranged by size:
2573 1290 1156 327 204 28
BmgBI CAC'GTC
Cuts at: 1318 2517 1318
Size: 1199 4379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4267 442
Size: 416 53 83 399 24 1892 958 1753
Fragments arranged by size:
1892 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2341 4238 1239
Size: 1102 1897 2579
Fragments arranged by size:
2579 1897 1102
Bpu10I CC'TnA_GC
Cuts at: 2243 2243
Size: 5578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 4568 4866 3703
Size: 865 298 4415
Fragments arranged by size:
4415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2359 2375 4241 882
Size: 617 860 16 1866 2219
Fragments arranged by size:
2219 1866 860 617 16
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2124 2163 2489
Size: 53 83 186 936 479 112 39 326
Cuts at: 2489 2742 3385 3767 4402 275
Size: 253 643 382 635 1451
Fragments arranged by size:
1451 936 643 635 479 382 326 253
186 112 83 53 39
BsaWI w'CCGG_w
Cuts at: 2484 2652 4011 4409 4683 4830 5241 2484
Size: 168 1359 398 274 147 411 2821
Fragments arranged by size:
2821 1359 411 398 274 168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5353
Fragments arranged by size:
5353 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2699 2852 1186
Size: 1513 153 3912
Fragments arranged by size:
3912 1513 153
BseYI C'CCAG_C
Cuts at: 4781 5277 4781
Size: 496 5082
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 2777 1163
Size: 573 816 225 3964
Fragments arranged by size:
3964 816 573 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2433 3028 3789 4817 1540
Size: 275 186 432 595 761 1028 2301
Fragments arranged by size:
2301 1028 761 595 432 275 186
BsiHKAI G_wGCw'C
Cuts at: 3639 3724 4795 3639
Size: 85 1071 4422
Fragments arranged by size:
4422 1071 85
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
BsmBI CGTCTCn'nnnn_
Cuts at: 2181 2181
Size: 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2117 2145 2349 2897 3639 3724
Size: 399 259 68 28 204 548 742 85
Cuts at: 3724 4795 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 548 399 259 204 85
68 28
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 2933 844
Size: 1776 313 3489
Fragments arranged by size:
3489 1776 313
BsrBI CCG'CTC
Cuts at: 1394 1823 2689 2701 2785 3465 1394
Size: 429 866 12 84 680 3507
Fragments arranged by size:
3507 866 680 429 84 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2275 2715 4222 4409 1700
Size: 110 465 440 1507 187 2869
Fragments arranged by size:
2869 1507 465 440 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 2022 2205 2322 1925
Size: 97 183 117 5181
Fragments arranged by size:
5181 183 117 97
BssSI C'ACGA_G
Cuts at: 2042 3638 4650 2042
Size: 1596 1012 2970
Fragments arranged by size:
2970 1596 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2824 794
Size: 2030 3548
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 2134
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2070 2225 2535 2908 3275 3660 3677 5129
Size: 98 155 310 373 367 385 17 1452
Cuts at: 5129 5215 5227 5573 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 373 367 346 310 155
98 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2108 2349 513
Size: 552 939 104 241 3742
Fragments arranged by size:
3742 939 552 241 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2546 487
Size: 683 56 1320 3519
Fragments arranged by size:
3519 1320 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3888 3908 3094
Size: 794 20 4764
Fragments arranged by size:
4764 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2630 4585 809
Size: 507 1314 1955 1802
Fragments arranged by size:
1955 1802 1314 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 2467 3025
Size: 54 1425 50 275 18 600 37 558
Cuts at: 3025 3914 5543 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 600 558 275 54 50
43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2430 3025 1537
Size: 275 618 595 4090
Fragments arranged by size:
4090 618 595 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3508 1353
Size: 2155 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2696 2711 4165 4539 4685 387
Size: 1064 138 1107 15 1454 374 146 1280
Fragments arranged by size:
1454 1280 1107 1064 374 146 138 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2341 3641 4238 5025 5293 1239
Size: 393 709 1300 597 787 268 1524
Fragments arranged by size:
1524 1300 787 709 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2113 2114 2141 2142 2113
Size: 1 27 1 5549
Fragments arranged by size:
5549 27 1 1
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2015 2127 2166 2255 2557 2600 4725 1678
Size: 337 112 39 89 302 43 2125 2531
Fragments arranged by size:
2531 2125 337 302 112 89 43 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 4219 4251 4293
Size: 32 135 32 638 32 1977 32 42
Cuts at: 4293 4325 1373
Size: 32 2626
Fragments arranged by size:
2626 1977 638 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KasI G'GCGC_C
Cuts at: 2011 2123 2162 2011
Size: 112 39 5427
Fragments arranged by size:
5427 112 39
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4692 4876 1305
Size: 3387 184 2007
Fragments arranged by size:
3387 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3536 3895 4054 518
Size: 648 2370 359 159 2042
Fragments arranged by size:
2370 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2290 2426 2731 2861 2994 3671 4819
Size: 504 180 136 305 130 133 677 1148
Cuts at: 4819 5064 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 504 305 245 180 136
133 130
NaeI GCC'GGC
Cuts at: 2277 2717 2277
Size: 440 5138
NarI GG'CG_CC
Cuts at: 2012 2124 2163 2012
Size: 112 39 5427
Fragments arranged by size:
5427 112 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4087
Fragments arranged by size:
4087 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NgoMIV G'CCGG_C
Cuts at: 2275 2715 2275
Size: 440 5138
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 2430 3025 2430
Size: 595 4983
NspI r_CATG'y
Cuts at: 1132 1229 2936 4481 1132
Size: 97 1707 1545 2229
Fragments arranged by size:
2229 1707 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2832 1348
Size: 1484 4094
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PspOMI G'GGCC_C
Cuts at: 2113 2141 2113
Size: 28 5550
PstI C_TGCA'G
Cuts at: 830 2730 2820 2829 2930 830
Size: 1900 90 9 101 3478
Fragments arranged by size:
3478 1900 101 90 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2731 2731
Size: 5578
SacII CC_GC'GG
Cuts at: 2111 2111
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SexAI A'CCwGG_T
Cuts at: 2639 2639
Size: 5578
SfcI C'TryA_G
Cuts at: 826 1045 2726 2816 2825 2926 4061 4742 4933
Size: 219 1681 90 9 101 1135 681 191
Cuts at: 4933 826
Size: 1471
Fragments arranged by size:
1681 1471 1135 681 219 191 101 90
9
SfoI GGC'GCC
Cuts at: 2013 2125 2164 2013
Size: 112 39 5427
Fragments arranged by size:
5427 112 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2149 2296 2528 2905 1872
Size: 277 147 232 377 4545
Fragments arranged by size:
4545 377 277 232 147
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3682 4583 4845 820
Size: 197 926 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2004 2362 2850 3017 513
Size: 552 939 358 488 167 3074
Fragments arranged by size:
3074 939 552 488 358 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3605 3622 3775 3960 4405 325
Size: 1255 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3824 5536 96
Size: 274 80 33 51 1443 1847 1712 138
Fragments arranged by size:
1847 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5538 5570 5538
Size: 32 5546
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2147 2294 2526 2903 1870
Size: 277 147 232 377 4545
Fragments arranged by size:
4545 377 277 232 147
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI
XcmI