Restriction map for
pFN21AB7584
(Circular) (Six-base) MAPSORT of: pFN21AB7584.seq Check: 9752 from: 1 to: 5566
pFN21AB7584 5566 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2909 1937
Size: 972 4594
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 3693 4066 1563
Size: 2130 373 3063
Fragments arranged by size:
3063 2130 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3629 5013 5281 1632
Size: 1997 1384 268 1917
Fragments arranged by size:
1997 1917 1384 268
AfeI AGC'GCT
Cuts at: 2137 2137
Size: 5566
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5369
AflIII A'CryG_T
Cuts at: 1203 4465 5442 1203
Size: 3262 977 1327
Fragments arranged by size:
3262 1327 977
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 2329 4295 2329
Size: 1966 3600
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2711 1184
Size: 1527 4039
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 5534
AlwNI CAG_nnn'CTG
Cuts at: 2311 2464 2773 3309 4881 2311
Size: 153 309 536 1572 2996
Fragments arranged by size:
2996 1572 536 309 153
ApaI G_GGCC'C
Cuts at: 2841 2841
Size: 5566
ApaLI G'TGCA_C
Cuts at: 3623 4779 3623
Size: 1156 4410
ApoI r'AATT_y
Cuts at: 1493 2875 3101 3253 5362 1493
Size: 1382 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4120 160
Size: 872 3088 1606
Fragments arranged by size:
3088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2158 2474 2834 2891 1870
Size: 73 159 56 316 360 57 4545
Fragments arranged by size:
4545 360 316 159 73 57 56
BamHI G'GATC_C
Cuts at: 2896 3263 2896
Size: 367 5199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 2887 4342 618
Size: 325 217 627 794 306 1455 1842
Fragments arranged by size:
1842 1455 794 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2081 2841 2885 1391
Size: 690 760 44 4072
Fragments arranged by size:
4072 760 690 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 928
Size: 971 201 4394
Fragments arranged by size:
4394 971 201
BbvCI CC'TCA_GC
Cuts at: 2805 2805
Size: 5566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3757 3791 1903
Size: 34 1820 34 3678
Fragments arranged by size:
3678 1820 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 2489 4177
Size: 107 122 71 631 605 404 413 1688
Cuts at: 4177 136
Size: 1525
Fragments arranged by size:
1688 1525 631 605 413 404 122 107
71
BglII A'GATC_T
Cuts at: 5561 5561
Size: 5566
BlpI GC'TnA_GC
Cuts at: 2030 2983 2030
Size: 953 4613
Bme1580I G_kGCm'C
Cuts at: 1790 2841 3627 4783 1790
Size: 1051 786 1156 2573
Fragments arranged by size:
2573 1156 1051 786
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2321 2452 3297
Size: 416 53 83 399 24 904 131 845
Cuts at: 3297 4255 442
Size: 958 1753
Fragments arranged by size:
1753 958 904 845 416 399 131 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2398 2609 4226 1239
Size: 799 360 211 1617 2579
Fragments arranged by size:
2579 1617 799 360 211
Bpu10I CC'TnA_GC
Cuts at: 2063 2805 2063
Size: 742 4824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3691 4556 4854 3691
Size: 865 298 4403
Fragments arranged by size:
4403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4229 882
Size: 617 2730 2219
Fragments arranged by size:
2730 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3357 493
Size: 711 2153 2702
Fragments arranged by size:
2702 2153 711
BsaBI GATnn'nnATC
Cuts at: 5560 5560
Size: 5566
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3373 3755 4390 275
Size: 53 83 186 936 1840 382 635 1451
Fragments arranged by size:
1840 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3999 4397 4671 4818 5229 3999
Size: 398 274 147 411 4336
Fragments arranged by size:
4336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2705 2735 1178
Size: 30 165 30 855 30 417 30 4009
Fragments arranged by size:
4009 855 417 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 1186
Size: 1171 4395
BseYI C'CCAG_C
Cuts at: 2142 2500 2726 4769 5265 2142
Size: 358 226 2043 496 2443
Fragments arranged by size:
2443 2043 496 358 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 1163
Size: 573 1068 3925
Fragments arranged by size:
3925 1068 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2023 3016 3777 4805 1540
Size: 275 186 22 993 761 1028 2301
Fragments arranged by size:
2301 1028 993 761 275 186 22
BsiHKAI G_wGCw'C
Cuts at: 2595 3627 3712 4783 2595
Size: 1032 85 1071 3378
Fragments arranged by size:
3378 1071 1032 85
BsmI GAATG_Cn'
Cuts at: 3077 3170 3077
Size: 93 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2595 2841 2885 3627 3712 4783
Size: 399 291 514 246 44 742 85 1071
Cuts at: 4783 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 514 399 291 246 85
44
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2921 844
Size: 2077 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2707 3453 1394
Size: 429 884 746 3507
Fragments arranged by size:
3507 884 746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4061 4235 65
Size: 1183 2813 174 1396
Fragments arranged by size:
2813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 4210 4397 1700
Size: 110 470 1930 187 2869
Fragments arranged by size:
2869 1930 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5566
BssHII G'CGCG_C
Cuts at: 1925 2479 1925
Size: 554 5012
BssSI C'ACGA_G
Cuts at: 3626 4638 3626
Size: 1012 4554
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2311 794
Size: 1517 4049
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstXI CCAn_nnnn'nTGG
Cuts at: 2171 2734 2171
Size: 563 5003
BstYI r'GATC_y
Cuts at: 1972 2896 3263 3648 3665 5117 5203 5215 5561
Size: 924 367 385 17 1452 86 12 346
Cuts at: 5561 1972
Size: 1977
Fragments arranged by size:
1977 1452 924 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2616 513
Size: 552 1551 3463
Fragments arranged by size:
3463 1551 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4827
Fragments arranged by size:
4827 683 56
BtsI GCAGTG_nn'
Cuts at: 3082 3876 3896 3082
Size: 794 20 4752
Fragments arranged by size:
4752 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2870 2870
Size: 5566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4573 809
Size: 507 3257 1802
Fragments arranged by size:
3257 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 2427 2490
Size: 54 1425 50 275 18 343 254 63
Cuts at: 2490 3013 3902 5531 8
Size: 523 889 1629 43
Fragments arranged by size:
1629 1425 889 523 343 275 254 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 1537
Size: 275 1201 4090
Fragments arranged by size:
4090 1201 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2665 3496 1353
Size: 1312 831 3423
Fragments arranged by size:
3423 1312 831
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4153 4527 4673 387
Size: 1064 138 2564 374 146 1280
Fragments arranged by size:
2564 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2398 2609 3629 4226 5013 5281
Size: 393 406 360 211 1020 597 787 268
Cuts at: 5281 1239
Size: 1524
Fragments arranged by size:
1524 1020 787 597 406 393 360 268
211
EcoO109I rG'GnC_Cy
Cuts at: 2323 2838 2323
Size: 515 5051
EcoRI G'AATT_C
Cuts at: 2875 3253 2875
Size: 378 5188
FspI TGC'GCA
Cuts at: 2363 4072 2363
Size: 1709 3857
FspAI rTGC'GCAy
Cuts at: 2363 2363
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 2139 2241 2443 2471 4713 1678
Size: 461 102 202 28 2242 2531
Fragments arranged by size:
2531 2242 461 202 102 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2315 2347 4207
Size: 32 135 32 569 32 142 32 1860
Cuts at: 4207 4239 4281 4313 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1860 569 142 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2910 3156 669
Size: 988 281 972 246 3079
Fragments arranged by size:
3079 988 972 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5566
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 4680 4864 1305
Size: 930 2445 184 2007
Fragments arranged by size:
2445 2007 930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2175 10
Size: 54 1425 343 343 3401
Fragments arranged by size:
3401 1425 343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3524 3883 4042 518
Size: 648 2358 359 159 2042
Fragments arranged by size:
2358 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 2425 2500 2587 2695 2982 3659 4807
Size: 705 114 75 87 108 287 677 1148
Cuts at: 4807 5052 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 705 677 287 245 114 108
87 75
NcoI C'CATG_G
Cuts at: 513 1065 2616 513
Size: 552 1551 3463
Fragments arranged by size:
3463 1551 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5566
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2924 4469 1132
Size: 97 1695 1545 2229
Fragments arranged by size:
2229 1695 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2852 1348
Size: 1504 4062
PciI A'CATG_T
Cuts at: 4465 4465
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2464 2754 1108
Size: 1356 290 3920
Fragments arranged by size:
3920 1356 290
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 5148 5180 3635
Size: 32 1481 32 4021
Fragments arranged by size:
4021 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2323
Size: 5566
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5566
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PspOMI G'GGCC_C
Cuts at: 2837 2837
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2439 2918 830
Size: 1609 479 3478
Fragments arranged by size:
3478 1609 479
PvuI CG_AT'CG
Cuts at: 2001 2023 2001
Size: 22 5544
PvuII CAG'CTG
Cuts at: 2425 2500 2695 2425
Size: 75 195 5296
Fragments arranged by size:
5296 195 75
SalI G'TCGA_C
Cuts at: 1936 2908 1936
Size: 972 4594
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SfcI C'TryA_G
Cuts at: 826 1045 2131 2435 2914 4049 4730 4921 826
Size: 219 1086 304 479 1135 681 191 1471
Fragments arranged by size:
1471 1135 1086 681 479 304 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2836 2893 1872
Size: 964 57 4545
Fragments arranged by size:
4545 964 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3670 4571 4833 820
Size: 197 926 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2924 1132
Size: 1792 3774
SspI AAT'ATT
Cuts at: 5 52 3490 5
Size: 47 3438 2081
Fragments arranged by size:
3438 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5566
StyI C'CwwG_G
Cuts at: 513 1065 2082 2616 3005 513
Size: 552 1017 534 389 3074
Fragments arranged by size:
3074 1017 552 534 389
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3593 3610 3763 3948 4393 325
Size: 1255 2013 17 153 185 445 1498
Fragments arranged by size:
2013 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3812 5524 96
Size: 274 80 33 51 1443 1835 1712 138
Fragments arranged by size:
1835 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5526 5558 5526
Size: 32 5534
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2422 2422
Size: 5566
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2834 2891 1870
Size: 964 57 4545
Fragments arranged by size:
4545 964 57
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI