Restriction map for
pFN21AB7580
(Circular) (Six-base) MAPSORT of: pFN21AB7580.seq Check: 8115 from: 1 to: 5557
pFN21AB7580 5557 bp
With 182 enzymes: SgfI *
March 28, 2012 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2488 2488
Size: 5557
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3620 5004 5272 1632
Size: 1988 1384 268 1917
Fragments arranged by size:
1988 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 4456 5433 1203
Size: 3253 977 1327
Fragments arranged by size:
3253 1327 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2510 1184
Size: 1326 4231
AlwNI CAG_nnn'CTG
Cuts at: 3300 4872 3300
Size: 1572 3985
ApaI G_GGCC'C
Cuts at: 2357 2357
Size: 5557
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2576 2866 3092 3244 5353 1493
Size: 1083 290 226 152 2109 1697
Fragments arranged by size:
2109 1697 1083 290 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2882 1870
Size: 73 104 835 4545
Fragments arranged by size:
4545 835 104 73
BamHI G'GATC_C
Cuts at: 2191 2887 3254 2191
Size: 696 367 4494
Fragments arranged by size:
4494 696 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2143 2429 2878 4333 618
Size: 325 217 627 356 286 449 1455 1842
Fragments arranged by size:
1842 1455 627 449 356 325 286 217
BanII G_rGCy'C
Cuts at: 1391 2066 2225 2357 2876 1391
Size: 675 159 132 519 4072
Fragments arranged by size:
4072 675 519 159 132
BbeI G_GCGC'C
Cuts at: 2433 2433
Size: 5557
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 928
Size: 971 354 4232
Fragments arranged by size:
4232 971 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2271 3449 4659 2181
Size: 90 1178 1210 3079
Fragments arranged by size:
3079 1210 1178 90
BclI T'GATC_A
Cuts at: 1548 2548 2697 1548
Size: 1000 149 4408
Fragments arranged by size:
4408 1000 149
BfrBI ATG'CAT
Cuts at: 2477 2477
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2070 2361 2391
Size: 107 122 71 631 605 398 291 30
Cuts at: 2391 2400 2438 4168 136
Size: 9 38 1730 1525
Fragments arranged by size:
1730 1525 631 605 398 291 122 107
71 38 30 9
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2288 2357 3618 4774 1790
Size: 264 234 69 1261 1156 2573
Fragments arranged by size:
2573 1261 1156 264 234 69
BmgBI CAC'GTC
Cuts at: 1318 2305 1318
Size: 987 4570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3288 4246
Size: 416 53 83 399 24 1293 578 958
Cuts at: 4246 442
Size: 1753
Fragments arranged by size:
1753 1293 958 578 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 4217 1239
Size: 1033 1945 2579
Fragments arranged by size:
2579 1945 1033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4220 882
Size: 617 2721 2219
Fragments arranged by size:
2721 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3348 493
Size: 711 2144 2702
Fragments arranged by size:
2702 2144 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2430 3364 3746 4381
Size: 53 83 186 936 897 934 382 635
Cuts at: 4381 275
Size: 1451
Fragments arranged by size:
1451 936 934 897 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3990 4388 4662 4809 5220 3990
Size: 398 274 147 411 4327
Fragments arranged by size:
4327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2724 2741 2754 2771 1178
Size: 30 165 30 1321 17 13 17 3964
Fragments arranged by size:
3964 1321 165 30 30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5557
BseYI C'CCAG_C
Cuts at: 2084 2183 2450 4760 5256 2084
Size: 99 267 2310 496 2385
Fragments arranged by size:
2385 2310 496 267 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 1163
Size: 573 780 4204
Fragments arranged by size:
4204 780 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2337 3007 3768 4796 1540
Size: 275 186 336 670 761 1028 2301
Fragments arranged by size:
2301 1028 761 670 336 275 186
BsiHKAI G_wGCw'C
Cuts at: 3618 3703 4774 3618
Size: 85 1071 4401
Fragments arranged by size:
4401 1071 85
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
BsmBI CGTCTCn'nnnn_
Cuts at: 2211 2211
Size: 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2066 2225 2288 2357 2876 3618
Size: 399 264 12 159 63 69 519 742
Cuts at: 3618 3703 4774 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 519 399 264 159 85
69 63 12
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 2058 3446 5176 2058
Size: 1388 1730 2439
Fragments arranged by size:
2439 1730 1388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 2830 2912 844
Size: 1644 342 82 3489
Fragments arranged by size:
3489 1644 342 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3444 1394
Size: 429 1621 3507
Fragments arranged by size:
3507 1621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2581 4052 4226 65
Size: 1183 1333 1471 174 1396
Fragments arranged by size:
1471 1396 1333 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2146 2293 4201 4388 1700
Size: 110 336 147 1908 187 2869
Fragments arranged by size:
2869 1908 336 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2191 2887 3254 3639 3656 5108 5194 5206
Size: 219 696 367 385 17 1452 86 12
Cuts at: 5206 5552 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 696 385 367 346 219 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5005
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 3073 3867 3887 3073
Size: 794 20 4743
Fragments arranged by size:
4743 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2201 3004 3893
Size: 54 1425 50 275 18 371 803 889
Cuts at: 3893 5522 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 803 371 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3004 1537
Size: 275 1192 4090
Fragments arranged by size:
4090 1192 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3487 1353
Size: 2134 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4144 4518 4664 387
Size: 1064 138 2555 374 146 1280
Fragments arranged by size:
2555 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 3620 4217 5004 5272 1239
Size: 393 640 1348 597 787 268 1524
Fragments arranged by size:
1524 1348 787 640 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2354 2354
Size: 5557
EcoRI G'AATT_C
Cuts at: 2576 2866 3244 2576
Size: 290 378 4889
Fragments arranged by size:
4889 378 290
FspI TGC'GCA
Cuts at: 2486 4063 2486
Size: 1577 3980
HaeII r_GCGC'y
Cuts at: 1678 2433 4704 1678
Size: 755 2271 2531
Fragments arranged by size:
2531 2271 755
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2245 2277 2294 2326 2741
Size: 32 135 32 673 32 17 32 415
Cuts at: 2741 2773 4198 4230 4272 4304 1373
Size: 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 673 415 135 42 32 32
32 32 32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2629 2901 3147 669
Size: 988 281 691 272 246 3079
Fragments arranged by size:
3079 988 691 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 2629 3147 2629
Size: 518 5039
KasI G'GCGC_C
Cuts at: 2429 2429
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4671 4855 1305
Size: 3366 184 2007
Fragments arranged by size:
3366 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3735
Fragments arranged by size:
3735 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 3515 3874 4033 518
Size: 648 891 1458 359 159 2042
Fragments arranged by size:
2042 1458 891 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2973 3650 4798 5043 1606
Size: 478 889 677 1148 245 2120
Fragments arranged by size:
2120 1148 889 677 478 245
NarI GG'CG_CC
Cuts at: 2430 2430
Size: 5557
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5005
NdeI CA'TA_TG
Cuts at: 387 2178 387
Size: 1791 3766
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NsiI A_TGCA'T
Cuts at: 2479 2479
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2044 2915 4460 1132
Size: 97 815 871 1545 2229
Fragments arranged by size:
2229 1545 871 815 97
PasI CC'CwG_GG
Cuts at: 1348 2066 2768 1348
Size: 718 702 4137
Fragments arranged by size:
4137 718 702
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PspOMI G'GGCC_C
Cuts at: 2353 2353
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2909 830
Size: 2079 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
PvuII CAG'CTG
Cuts at: 2084 2084
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2230 2341 2905 4040 4721 4912 826
Size: 219 1185 111 564 1135 681 191 1471
Fragments arranged by size:
1471 1185 1135 681 564 219 191 111
SfoI GGC'GCC
Cuts at: 2431 2431
Size: 5557
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2049 2884 1872
Size: 177 835 4545
Fragments arranged by size:
4545 835 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3661 4562 4824 820
Size: 197 926 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2436 2682 2996 513
Size: 552 1371 246 314 3074
Fragments arranged by size:
3074 1371 552 314 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2351 3584 3601 3754 3939 4384 325
Size: 1255 771 1233 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1233 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2276 3803 5515
Size: 274 80 33 51 1443 299 1527 1712
Cuts at: 5515 96
Size: 138
Fragments arranged by size:
1712 1527 1443 299 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2724 2756 5517 5549 2724
Size: 32 2761 32 2732
Fragments arranged by size:
2761 2732 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2047 2882 1870
Size: 177 835 4545
Fragments arranged by size:
4545 835 177
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI