Restriction map for
pFN21AB7579
(Circular) (Six-base) MAPSORT of: pFN21AB7579.seq Check: 6786 from: 1 to: 5365
pFN21AB7579 5365 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3175 278
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2708 1937
Size: 771 4594
Acc65I G'GTAC_C
Cuts at: 2686 2686
Size: 5365
AclI AA'CG_TT
Cuts at: 1563 3492 3865 1563
Size: 1929 373 3063
Fragments arranged by size:
3063 1929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 3428 4812 5080 1632
Size: 610 1186 1384 268 1917
Fragments arranged by size:
1917 1384 1186 610 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5168
AflIII A'CryG_T
Cuts at: 1203 4264 5241 1203
Size: 3061 977 1327
Fragments arranged by size:
3061 1327 977
AgeI A'CCGG_T
Cuts at: 4196 4196
Size: 5365
AhdI GACnn_n'nnGTC
Cuts at: 4094 4094
Size: 5365
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5365
AlwNI CAG_nnn'CTG
Cuts at: 2162 3108 4680 2162
Size: 946 1572 2847
Fragments arranged by size:
2847 1572 946
ApaI G_GGCC'C
Cuts at: 2169 2192 2169
Size: 23 5342
ApaLI G'TGCA_C
Cuts at: 3422 4578 3422
Size: 1156 4209
ApoI r'AATT_y
Cuts at: 1493 2674 2900 3052 5161 1493
Size: 1181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1181 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3919 160
Size: 872 2887 1606
Fragments arranged by size:
2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
AvaI C'yCGr_G
Cuts at: 1870 1943 2170 2192 2690 1870
Size: 73 227 22 498 4545
Fragments arranged by size:
4545 498 227 73 22
BamHI G'GATC_C
Cuts at: 2268 2695 3062 2268
Size: 427 367 4571
Fragments arranged by size:
4571 427 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2134 2686 4141 618
Size: 325 217 627 333 14 552 1455 1842
Fragments arranged by size:
1842 1455 627 552 333 325 217 14
BanII G_rGCy'C
Cuts at: 1391 2169 2192 2684 1391
Size: 778 23 492 4072
Fragments arranged by size:
4072 778 492 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4394
BbvCI CC'TCA_GC
Cuts at: 2130 2130
Size: 5365
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3556 3590 1903
Size: 34 1619 34 3678
Fragments arranged by size:
3678 1619 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3257 4467 3257
Size: 1210 4155
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2096 3976
Size: 107 122 71 631 605 388 36 1880
Cuts at: 3976 136
Size: 1525
Fragments arranged by size:
1880 1525 631 605 388 122 107 71
36
BglII A'GATC_T
Cuts at: 2638 5360 2638
Size: 2722 2643
BlpI GC'TnA_GC
Cuts at: 2782 2782
Size: 5365
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2169 2192 3426 4582 1790
Size: 333 46 23 1234 1156 2573
Fragments arranged by size:
2573 1234 1156 333 46 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3096 4054 442
Size: 416 53 83 399 24 1679 958 1753
Fragments arranged by size:
1753 1679 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4025 1239
Size: 2786 2579
Bpu10I CC'TnA_GC
Cuts at: 2130 2130
Size: 5365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 3490 4355 4653 2627
Size: 863 865 298 3339
Fragments arranged by size:
3339 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2158 4028 882
Size: 617 659 1870 2219
Fragments arranged by size:
2219 1870 659 617
BsaAI yAC'GTr
Cuts at: 493 1204 3156 493
Size: 711 1952 2702
Fragments arranged by size:
2702 1952 711
BsaBI GATnn'nnATC
Cuts at: 5359 5359
Size: 5365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3172 3554 4189 275
Size: 53 83 186 936 1639 382 635 1451
Fragments arranged by size:
1639 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3798 4196 4470 4617 5028 3798
Size: 398 274 147 411 4135
Fragments arranged by size:
4135 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5140
Fragments arranged by size:
5140 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5365
BseYI C'CCAG_C
Cuts at: 2162 4568 5064 2162
Size: 2406 496 2463
Fragments arranged by size:
2463 2406 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4792
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2085 2181 2236 2815 3576
Size: 275 186 51 33 96 55 579 761
Cuts at: 3576 4604 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 579 275 186 96 55
51 33
BsiHKAI G_wGCw'C
Cuts at: 3426 3511 4582 3426
Size: 85 1071 4209
Fragments arranged by size:
4209 1071 85
BsmI GAATG_Cn'
Cuts at: 2876 2969 2876
Size: 93 5272
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2169 2192 2684 3426 3511 4582
Size: 399 333 46 23 492 742 85 1071
Cuts at: 4582 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 492 399 333 85 46
23
BspEI T'CCGG_A
Cuts at: 5028 5028
Size: 5365
BspHI T'CATG_A
Cuts at: 3254 4984 3254
Size: 1730 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2720 844
Size: 1876 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2227 3252 1394
Size: 429 404 1025 3507
Fragments arranged by size:
3507 1025 429 404
BsrDI GCAATG_nn'
Cuts at: 65 1248 3860 4034 65
Size: 1183 2612 174 1396
Fragments arranged by size:
2612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 4009 4196 1700
Size: 110 301 1898 187 2869
Fragments arranged by size:
2869 1898 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5365
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5365
BssSI C'ACGA_G
Cuts at: 3425 4437 3425
Size: 1012 4353
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5365
BstBI TT'CG_AA
Cuts at: 2371 4169 2371
Size: 1798 3567
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5365
BstYI r'GATC_y
Cuts at: 1972 2268 2638 2695 3062 3447 3464 4916 5002
Size: 296 370 57 367 385 17 1452 86
Cuts at: 5002 5014 5360 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 370 367 346 296 86
57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2035 2079 513
Size: 552 970 44 3799
Fragments arranged by size:
3799 970 552 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4626
Fragments arranged by size:
4626 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2073 2881 3675 3695 2064
Size: 9 808 794 20 3734
Fragments arranged by size:
3734 808 794 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5330
DraI TTT'AAA
Cuts at: 2669 2669
Size: 5365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4372 809
Size: 507 3056 1802
Fragments arranged by size:
3056 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2061 2082
Size: 54 1425 50 275 18 219 12 21
Cuts at: 2082 2178 2233 2812 3701 5330 8
Size: 96 55 579 889 1629 43
Fragments arranged by size:
1629 1425 889 579 275 219 96 55
54 50 43 21 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2049 2082 2178 2233 2812 1537
Size: 275 237 33 96 55 579 4090
Fragments arranged by size:
4090 579 275 237 96 55 33
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 3295 1353
Size: 917 1025 3423
Fragments arranged by size:
3423 1025 917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3952 4326 4472 387
Size: 1064 138 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 3428 4025 4812 5080 1239
Size: 393 610 1186 597 787 268 1524
Fragments arranged by size:
1524 1186 787 610 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2213 2213
Size: 5365
EcoO109I rG'GnC_Cy
Cuts at: 2166 2189 2166
Size: 23 5342
EcoRI G'AATT_C
Cuts at: 2674 3052 2674
Size: 378 4987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2552 2552
Size: 5365
FspI TGC'GCA
Cuts at: 3871 3871
Size: 5365
HaeII r_GCGC'y
Cuts at: 1678 4512 1678
Size: 2834 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373
Size: 32 135 32 2434 32 42 32 2626
Fragments arranged by size:
2626 2434 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2709 2955 669
Size: 988 281 771 246 3079
Fragments arranged by size:
3079 988 771 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5365
HpaI GTT'AAC
Cuts at: 2955 2955
Size: 5365
KpnI G_GTAC'C
Cuts at: 2690 2690
Size: 5365
MfeI C'AATT_G
Cuts at: 2964 2964
Size: 5365
MluI A'CGCG_T
Cuts at: 5241 5241
Size: 5365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4479 4663 1305
Size: 3174 184 2007
Fragments arranged by size:
3174 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3543
Fragments arranged by size:
3543 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3323 3682 3841 518
Size: 648 2157 359 159 2042
Fragments arranged by size:
2157 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2081 2159 2781 3458 4606 4851 1606
Size: 431 44 78 622 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 622 431 245 78 44
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4813
NdeI CA'TA_TG
Cuts at: 387 2336 387
Size: 1949 3416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5365
NotI GC'GGCC_GC
Cuts at: 2049 2082 2812 2049
Size: 33 730 4602
Fragments arranged by size:
4602 730 33
NspI r_CATG'y
Cuts at: 1132 1229 2723 4268 1132
Size: 97 1494 1545 2229
Fragments arranged by size:
2229 1545 1494 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5365
PciI A'CATG_T
Cuts at: 4264 4264
Size: 5365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5365
PmeI GTTT'AAAC
Cuts at: 2669 2669
Size: 5365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4947 4979 3434
Size: 32 1481 32 3820
Fragments arranged by size:
3820 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5365
PsiI TTA'TAA
Cuts at: 2935 2935
Size: 5365
PspOMI G'GGCC_C
Cuts at: 2165 2188 2165
Size: 23 5342
PstI C_TGCA'G
Cuts at: 830 2717 830
Size: 1887 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5365
SacII CC_GC'GG
Cuts at: 2038 2082 2038
Size: 44 5321
SalI G'TCGA_C
Cuts at: 1936 2707 1936
Size: 771 4594
SapI GCTCTTCn'nnn_
Cuts at: 2270 2270
Size: 5365
SbfI CC_TGCA'GG
Cuts at: 2717 2717
Size: 5365
ScaI AGT'ACT
Cuts at: 3613 5325 3613
Size: 1712 3653
SfcI C'TryA_G
Cuts at: 826 1045 2713 3848 4529 4720 826
Size: 219 1668 1135 681 191 1471
Fragments arranged by size:
1668 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5365
SmaI CCC'GGG
Cuts at: 1872 2172 2194 2692 1872
Size: 300 22 498 4545
Fragments arranged by size:
4545 498 300 22
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 3469 4370 4632 820
Size: 197 926 699 827 901 262 1553
Fragments arranged by size:
1553 926 901 827 699 262 197
SnaBI TAC'GTA
Cuts at: 493 3156 493
Size: 2663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5365
SphI G_CATG'C
Cuts at: 1132 2723 1132
Size: 1591 3774
SspI AAT'ATT
Cuts at: 5 52 3289 5
Size: 47 3237 2081
Fragments arranged by size:
3237 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5365
StyI C'CwwG_G
Cuts at: 513 1065 2804 513
Size: 552 1739 3074
Fragments arranged by size:
3074 1739 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3392 3409 3562 3747 4192 325
Size: 1255 1812 17 153 185 445 1498
Fragments arranged by size:
1812 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3611 5323 96
Size: 274 80 33 51 1443 1634 1712 138
Fragments arranged by size:
1712 1634 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5325 5357 5325
Size: 32 5333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5365
XbaI T'CTAG_A
Cuts at: 2701 2701
Size: 5365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5365
XmaI C'CCGG_G
Cuts at: 1870 2170 2192 2690 1870
Size: 300 22 498 4545
Fragments arranged by size:
4545 498 300 22
XmnI GAAnn'nnTTC
Cuts at: 2012 3494 2012
Size: 1482 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3173 276
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI
XcmI