Restriction map for
pFN21AB7576
(Circular) (Six-base) MAPSORT of: pFN21AB7576.seq Check: 664 from: 1 to: 5557
pFN21AB7576 5557 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2861 3620 5004 5272 1632
Size: 1229 759 1384 268 1917
Fragments arranged by size:
1917 1384 1229 759 268
AflII C'TTAA_G
Cuts at: 820 1017 2756 820
Size: 197 1739 3621
Fragments arranged by size:
3621 1739 197
AflIII A'CryG_T
Cuts at: 1203 2360 4456 5433 1203
Size: 1157 2096 977 1327
Fragments arranged by size:
2096 1327 1157 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 3300 4872 3300
Size: 1572 3985
ApaI G_GGCC'C
Cuts at: 2793 2793
Size: 5557
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2866 3092 3244 5353 1493
Size: 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2786 2882 1870
Size: 73 843 96 4545
Fragments arranged by size:
4545 843 96 73
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2183 2489 2878 4333
Size: 325 217 627 291 105 306 389 1455
Cuts at: 4333 618
Size: 1842
Fragments arranged by size:
1842 1455 627 389 325 306 291 217
105
BanII G_rGCy'C
Cuts at: 1391 2624 2651 2793 2876 1391
Size: 1233 27 142 83 4072
Fragments arranged by size:
4072 1233 142 83 27
BbeI G_GCGC'C
Cuts at: 2082 2187 2493 2082
Size: 105 306 5146
Fragments arranged by size:
5146 306 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2849 928
Size: 971 950 3636
Fragments arranged by size:
3636 971 950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 2094 2708
Size: 107 122 71 631 605 382 40 614
Cuts at: 2708 4168 136
Size: 1460 1525
Fragments arranged by size:
1525 1460 631 614 605 382 122 107
71 40
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2793 3618 4774 1790
Size: 436 567 825 1156 2573
Fragments arranged by size:
2573 1156 825 567 436
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2654 3288 4246
Size: 416 53 83 399 24 1237 634 958
Cuts at: 4246 442
Size: 1753
Fragments arranged by size:
1753 1237 958 634 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 4217 1239
Size: 1100 1878 2579
Fragments arranged by size:
2579 1878 1100
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2363 2669 3682 4547 4845 2294
Size: 69 306 1013 865 298 3006
Fragments arranged by size:
3006 1013 865 306 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 4220 882
Size: 617 831 1890 2219
Fragments arranged by size:
2219 1890 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 2547 3348 493
Size: 711 1343 801 2702
Fragments arranged by size:
2702 1343 801 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2079 2184 2490
Size: 53 83 186 936 524 22 105 306
Cuts at: 2490 3364 3746 4381 275
Size: 874 382 635 1451
Fragments arranged by size:
1451 936 874 635 524 382 306 186
105 83 53 22
BsaWI w'CCGG_w
Cuts at: 2820 3990 4388 4662 4809 5220 2820
Size: 1170 398 274 147 411 3157
Fragments arranged by size:
3157 1170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 1178
Size: 30 165 30 1128 30 4174
Fragments arranged by size:
4174 1128 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2155 2381 2506 1186
Size: 969 226 125 4237
Fragments arranged by size:
4237 969 226 125
BseYI C'CCAG_C
Cuts at: 2624 4760 5256 2624
Size: 2136 496 2925
Fragments arranged by size:
2925 2136 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 2426 2569 1163
Size: 573 504 186 143 4151
Fragments arranged by size:
4151 573 504 186 143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2748 3007 3768 4796 1540
Size: 275 186 747 259 761 1028 2301
Fragments arranged by size:
2301 1028 761 747 275 259 186
BsiHKAI G_wGCw'C
Cuts at: 3618 3703 4774 3618
Size: 85 1071 4401
Fragments arranged by size:
4401 1071 85
BsiWI C'GTAC_G
Cuts at: 2028 2028
Size: 5557
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2624 2651 2793 2876 3618 3703
Size: 399 436 398 27 142 83 742 85
Cuts at: 3703 4774 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 398 142 85
83 27
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2398 2912 844
Size: 1554 514 3489
Fragments arranged by size:
3489 1554 514
BsrBI CCG'CTC
Cuts at: 1394 1823 2683 2744 3444 1394
Size: 429 860 61 700 3507
Fragments arranged by size:
3507 860 700 429 61
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2702 2770 4201 4388 1700
Size: 110 892 68 1431 187 2869
Fragments arranged by size:
2869 1431 892 187 110 68
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 2301 2452 2507 1925
Size: 376 151 55 4975
Fragments arranged by size:
4975 376 151 55
BssSI C'ACGA_G
Cuts at: 2035 2258 3617 4629 2035
Size: 223 1359 1012 2963
Fragments arranged by size:
2963 1359 1012 223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552
Size: 915 367 385 17 1452 86 12 346
Cuts at: 5552 1972
Size: 1977
Fragments arranged by size:
1977 1452 915 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2133 513
Size: 552 939 45 84 3937
Fragments arranged by size:
3937 939 552 84 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 3073 3867 3887 2226
Size: 847 794 20 3896
Fragments arranged by size:
3896 847 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2457 2604
Size: 54 1425 50 275 18 216 411 147
Cuts at: 2604 2745 3004 3893 5522 8
Size: 141 259 889 1629 43
Fragments arranged by size:
1629 1425 889 411 275 259 216 147
141 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2745 3004 1537
Size: 275 933 259 4090
Fragments arranged by size:
4090 933 275 259
EarI CTCTTCn'nnn_
Cuts at: 1353 2858 3487 1353
Size: 1505 629 3423
Fragments arranged by size:
3423 1505 629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2050 2789 4144 4518 4664 387
Size: 1064 138 461 739 1355 374 146 1280
Fragments arranged by size:
1355 1280 1064 739 461 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2861 3620 4217 5004 5272 1239
Size: 393 707 522 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 707 597 522 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2393 2393
Size: 5557
EcoO109I rG'GnC_Cy
Cuts at: 2660 2790 2660
Size: 130 5427
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
FspI TGC'GCA
Cuts at: 2176 4063 2176
Size: 1887 3670
HaeII r_GCGC'y
Cuts at: 1678 2082 2187 2378 2466 2493 4704 1678
Size: 404 105 191 88 27 2211 2531
Fragments arranged by size:
2531 2211 404 191 105 88 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373
Size: 32 135 32 2626 32 42 32 2626
Fragments arranged by size:
2626 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KasI G'GCGC_C
Cuts at: 2078 2183 2489 2078
Size: 105 306 5146
Fragments arranged by size:
5146 306 105
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 2360 5433 2360
Size: 3073 2484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 4671 4855 1305
Size: 888 2478 184 2007
Fragments arranged by size:
2478 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2606 10
Size: 54 1425 343 774 2961
Fragments arranged by size:
2961 1425 774 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3515 3874 4033 518
Size: 648 2349 359 159 2042
Fragments arranged by size:
2349 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 2275 2632 2973 3650 4798 5043 1606
Size: 529 140 357 341 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 529 357 341 245 140
NaeI GCC'GGC
Cuts at: 2772 2772
Size: 5557
NarI GG'CG_CC
Cuts at: 2079 2184 2490 2079
Size: 105 306 5146
Fragments arranged by size:
5146 306 105
NcoI C'CATG_G
Cuts at: 513 1065 2004 2049 513
Size: 552 939 45 4021
Fragments arranged by size:
4021 939 552 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NgoMIV G'CCGG_C
Cuts at: 2770 2770
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2915 4460 1132
Size: 97 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PmlI CAC'GTG
Cuts at: 2547 2547
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2660 2660
Size: 5557
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PspOMI G'GGCC_C
Cuts at: 2789 2789
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2087 2909 830
Size: 1257 822 3478
Fragments arranged by size:
3478 1257 822
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
SacII CC_GC'GG
Cuts at: 2136 2136
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2083 2905 4040 4721 4912 826
Size: 219 1038 822 1135 681 191 1471
Fragments arranged by size:
1471 1135 1038 822 681 219 191
SfoI GGC'GCC
Cuts at: 2080 2185 2491 2080
Size: 105 306 5146
Fragments arranged by size:
5146 306 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2788 2884 1872
Size: 916 96 4545
Fragments arranged by size:
4545 916 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 2378 2684 2756 3661 4562
Size: 197 926 366 69 306 72 905 901
Cuts at: 4562 4824 820
Size: 262 1553
Fragments arranged by size:
1553 926 905 901 366 306 262 197
72 69
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2466 2535 2996 513
Size: 552 939 45 417 69 461 3074
Fragments arranged by size:
3074 939 552 461 417 69 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3584 3601 3754 3939 4384 325
Size: 1255 2004 17 153 185 445 1498
Fragments arranged by size:
2004 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 5517 5549 2531
Size: 32 2954 32 2539
Fragments arranged by size:
2954 2539 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2786 2882 1870
Size: 916 96 4545
Fragments arranged by size:
4545 916 96
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI XcmI