Restriction map for
pFN21AB7572
(Circular) (Six-base) MAPSORT of: pFN21AB7572.seq Check: 5050 from: 1 to: 5545
pFN21AB7572 5545 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2116 2116
Size: 5545
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 2888 1937
Size: 406 545 4594
Fragments arranged by size:
4594 545 406
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 2499 3672 4045 1563
Size: 936 1173 373 3063
Fragments arranged by size:
3063 1173 936 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3608 4992 5260 1632
Size: 1976 1384 268 1917
Fragments arranged by size:
1976 1917 1384 268
AfeI AGC'GCT
Cuts at: 2483 2483
Size: 5545
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 4274 4274
Size: 5545
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5545
AlwNI CAG_nnn'CTG
Cuts at: 2087 3288 4860 2087
Size: 1201 1572 2772
Fragments arranged by size:
2772 1572 1201
ApaI G_GGCC'C
Cuts at: 2039 2121 2039
Size: 82 5463
ApaLI G'TGCA_C
Cuts at: 2684 3602 4758 2684
Size: 918 1156 3471
Fragments arranged by size:
3471 1156 918
ApoI r'AATT_y
Cuts at: 1493 2854 3080 3232 5341 1493
Size: 1361 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 2870 1870
Size: 73 720 207 4545
Fragments arranged by size:
4545 720 207 73
BamHI G'GATC_C
Cuts at: 2717 2875 3242 2717
Size: 158 367 5020
Fragments arranged by size:
5020 367 158
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2433 2507 2705 2866 4321
Size: 325 217 627 646 74 198 161 1455
Cuts at: 4321 618
Size: 1842
Fragments arranged by size:
1842 1455 646 627 325 217 198 161
74
BanII G_rGCy'C
Cuts at: 1391 2039 2121 2834 2864 1391
Size: 648 82 713 30 4072
Fragments arranged by size:
4072 713 648 82 30
BbeI G_GCGC'C
Cuts at: 2511 2709 2511
Size: 198 5347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4574
BbvCI CC'TCA_GC
Cuts at: 2427 2522 2427
Size: 95 5450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2730 2764 3736 3770 1903
Size: 34 793 34 972 34 3678
Fragments arranged by size:
3678 972 793 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4647 3437
Size: 1210 4335
BclI T'GATC_A
Cuts at: 1548 2190 1548
Size: 642 4903
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2167 2738 4156
Size: 107 122 71 631 605 495 571 1418
Cuts at: 4156 136
Size: 1525
Fragments arranged by size:
1525 1418 631 605 571 495 122 107
71
BglII A'GATC_T
Cuts at: 5540 5540
Size: 5545
BlpI GC'TnA_GC
Cuts at: 2561 2962 2561
Size: 401 5144
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2039 2074 2121 2249 2436 2688 3606
Size: 222 27 35 47 128 187 252 918
Cuts at: 3606 4762 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 918 252 222 187 128 47
35 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3276 4234 442
Size: 416 53 83 399 24 1859 958 1753
Fragments arranged by size:
1859 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4205 1239
Size: 2966 2579
Bpu10I CC'TnA_GC
Cuts at: 2100 2427 2522 2100
Size: 327 95 5123
Fragments arranged by size:
5123 327 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 4535 4833 3670
Size: 865 298 4382
Fragments arranged by size:
4382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2392 4208 882
Size: 617 893 1816 2219
Fragments arranged by size:
2219 1816 893 617
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2508 2706 3352 3734
Size: 53 83 186 936 975 198 646 382
Cuts at: 3734 4369 275
Size: 635 1451
Fragments arranged by size:
1451 975 936 646 635 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 2553 2720 3978 4376 4650 4797 5208 2553
Size: 167 1258 398 274 147 411 2890
Fragments arranged by size:
2890 1258 411 398 274 167 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5320
Fragments arranged by size:
5320 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2147 2163 1186
Size: 960 1 16 4568
Fragments arranged by size:
4568 960 16 1
BseYI C'CCAG_C
Cuts at: 2075 2080 2242 4748 5244 2075
Size: 5 162 2506 496 2376
Fragments arranged by size:
2506 2376 496 162 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 1163
Size: 573 558 4414
Fragments arranged by size:
4414 573 558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2554 2681 2769 2995 3756 4784
Size: 275 186 553 127 88 226 761 1028
Cuts at: 4784 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 553 275 226 186 127
88
BsiHKAI G_wGCw'C
Cuts at: 2580 2688 3606 3691 4762 2580
Size: 108 918 85 1071 3363
Fragments arranged by size:
3363 1071 918 108 85
BsmI GAATG_Cn'
Cuts at: 3056 3149 3056
Size: 93 5452
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2039 2074 2121 2249 2436 2580
Size: 399 222 27 35 47 128 187 144
Cuts at: 2580 2688 2834 2864 3606 3691 4762 1391
Size: 108 146 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 222 187 146 144
128 108 85 47 35 30 27
BspEI T'CCGG_A
Cuts at: 2720 5208 2720
Size: 2488 3057
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2116 2900 844
Size: 1272 784 3489
Fragments arranged by size:
3489 1272 784
BsrBI CCG'CTC
Cuts at: 1394 1823 2795 3432 1394
Size: 429 972 637 3507
Fragments arranged by size:
3507 972 637 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4040 4214 65
Size: 1183 2792 174 1396
Fragments arranged by size:
2792 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 2680 4189 4376 1700
Size: 110 575 295 1509 187 2869
Fragments arranged by size:
2869 1509 575 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 2744 1925
Size: 819 4726
BssSI C'ACGA_G
Cuts at: 3605 4617 3605
Size: 1012 4533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5545
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2812 2242
Size: 570 4975
BstYI r'GATC_y
Cuts at: 1972 2717 2875 3242 3627 3644 5096 5182 5194
Size: 745 158 367 385 17 1452 86 12
Cuts at: 5194 5540 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 745 385 367 346 158 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2016 2016
Size: 5545
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4993
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 3061 3855 3875 3061
Size: 794 20 4731
Fragments arranged by size:
4731 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DraIII CAC_nnn'GTG
Cuts at: 2180 2180
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4552 809
Size: 507 3236 1802
Fragments arranged by size:
3236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2643 2678 2766
Size: 54 1425 50 275 18 813 35 88
Cuts at: 2766 2992 3881 5510 8
Size: 226 889 1629 43
Fragments arranged by size:
1629 1425 889 813 275 226 88 54
50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2678 2766 2992 1537
Size: 275 866 88 226 4090
Fragments arranged by size:
4090 866 275 226 88
EarI CTCTTCn'nnn_
Cuts at: 1353 3475 1353
Size: 2122 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2570 4132 4506 4652 387
Size: 1064 138 981 1562 374 146 1280
Fragments arranged by size:
1562 1280 1064 981 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3608 4205 4992 5260 1239
Size: 393 1976 597 787 268 1524
Fragments arranged by size:
1976 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2426 2426
Size: 5545
EcoO109I rG'GnC_Cy
Cuts at: 2036 2118 2211 2360 2651 2692 2036
Size: 82 93 149 291 41 4889
Fragments arranged by size:
4889 291 149 93 82 41
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 2087 2485 2511 2709 4692 1678
Size: 409 398 26 198 1983 2531
Fragments arranged by size:
2531 1983 409 398 198 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373
Size: 32 135 32 2614 32 42 32 2626
Fragments arranged by size:
2626 2614 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 2889 3135 669
Size: 988 281 565 386 246 3079
Fragments arranged by size:
3079 988 565 386 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 2503 3135 2503
Size: 632 4913
KasI G'GCGC_C
Cuts at: 2507 2705 2507
Size: 198 5347
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4659 4843 1305
Size: 3354 184 2007
Fragments arranged by size:
3354 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3723
Fragments arranged by size:
3723 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 3503 3862 4021 518
Size: 648 1644 693 359 159 2042
Fragments arranged by size:
2042 1644 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 2961 3638 4786 5031 1606
Size: 657 698 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 657 245
NarI GG'CG_CC
Cuts at: 2508 2706 2508
Size: 198 5347
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4993
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2903 4448 1132
Size: 97 1674 1545 2229
Fragments arranged by size:
2229 1674 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2216 2281 1348
Size: 868 65 4612
Fragments arranged by size:
4612 868 65
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2449 1108
Size: 1341 4204
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 5127 5159 3614
Size: 32 1481 32 4000
Fragments arranged by size:
4000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2360 2692 2360
Size: 332 5213
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PspOMI G'GGCC_C
Cuts at: 2035 2117 2035
Size: 82 5463
PstI C_TGCA'G
Cuts at: 830 2897 830
Size: 2067 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SanDI GG'GwC_CC
Cuts at: 2692 2692
Size: 5545
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SexAI A'CCwGG_T
Cuts at: 2176 2176
Size: 5545
SfcI C'TryA_G
Cuts at: 826 1045 2893 4028 4709 4900 826
Size: 219 1848 1135 681 191 1471
Fragments arranged by size:
1848 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2509 2707 2509
Size: 198 5347
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2665 2872 1872
Size: 793 207 4545
Fragments arranged by size:
4545 793 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3649 4550 4812 820
Size: 197 926 1706 901 262 1553
Fragments arranged by size:
1706 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5545
StyI C'CwwG_G
Cuts at: 513 1065 2984 513
Size: 552 1919 3074
Fragments arranged by size:
3074 1919 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3572 3589 3742 3927 4372 325
Size: 1255 1992 17 153 185 445 1498
Fragments arranged by size:
1992 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3791 5503
Size: 274 80 33 51 1443 618 1196 1712
Cuts at: 5503 96
Size: 138
Fragments arranged by size:
1712 1443 1196 618 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5505 5537 5505
Size: 32 5513
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5545
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2239
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5545
XmaI C'CCGG_G
Cuts at: 1870 2663 2870 1870
Size: 793 207 4545
Fragments arranged by size:
4545 793 207
XmnI GAAnn'nnTTC
Cuts at: 3674 3674
Size: 5545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SapI SfiI SrfI SwaI