Restriction map for
pFN21AB7571
(Circular) (Six-base) MAPSORT of: pFN21AB7571.seq Check: 1525 from: 1 to: 5320
pFN21AB7571 5320 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3130 278
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2663 1937
Size: 726 4594
Acc65I G'GTAC_C
Cuts at: 2641 2641
Size: 5320
AclI AA'CG_TT
Cuts at: 1563 3447 3820 1563
Size: 1884 373 3063
Fragments arranged by size:
3063 1884 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3383 4767 5035 1632
Size: 602 1149 1384 268 1917
Fragments arranged by size:
1917 1384 1149 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5123
AflIII A'CryG_T
Cuts at: 1203 4219 5196 1203
Size: 3016 977 1327
Fragments arranged by size:
3016 1327 977
AgeI A'CCGG_T
Cuts at: 4151 4151
Size: 5320
AhdI GACnn_n'nnGTC
Cuts at: 4049 4049
Size: 5320
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5320
AlwNI CAG_nnn'CTG
Cuts at: 2450 2588 3063 4635 2450
Size: 138 475 1572 3135
Fragments arranged by size:
3135 1572 475 138
ApaI G_GGCC'C
Cuts at: 2579 2579
Size: 5320
ApaLI G'TGCA_C
Cuts at: 3377 4533 3377
Size: 1156 4164
ApoI r'AATT_y
Cuts at: 1493 2629 2855 3007 5116 1493
Size: 1136 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3874 160
Size: 872 2842 1606
Fragments arranged by size:
2842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 2129 2572 2645 1870
Size: 73 178 8 443 73 4545
Fragments arranged by size:
4545 443 178 73 73 8
BamHI G'GATC_C
Cuts at: 2650 3017 2650
Size: 367 4953
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2324 2641 4096 618
Size: 325 217 627 317 220 317 1455 1842
Fragments arranged by size:
1842 1455 627 325 317 317 220 217
BanII G_rGCy'C
Cuts at: 1391 2136 2519 2579 2639 1391
Size: 745 383 60 60 4072
Fragments arranged by size:
4072 745 383 60 60
BbeI G_GCGC'C
Cuts at: 2328 2328
Size: 5320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2198 2304 928
Size: 971 299 106 3944
Fragments arranged by size:
3944 971 299 106
BbvCI CC'TCA_GC
Cuts at: 2581 2581
Size: 5320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3511 3545 1903
Size: 34 50 34 1490 34 3678
Fragments arranged by size:
3678 1490 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3212 4422 3212
Size: 1210 4110
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3931 136
Size: 107 122 71 631 605 2259 1525
Fragments arranged by size:
2259 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5315 5315
Size: 5320
BlpI GC'TnA_GC
Cuts at: 2737 2737
Size: 5320
Bme1580I G_kGCm'C
Cuts at: 1790 2579 3381 4537 1790
Size: 789 802 1156 2573
Fragments arranged by size:
2573 1156 802 789
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5320
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2170 3051 4009
Size: 416 53 83 399 24 753 881 958
Cuts at: 4009 442
Size: 1753
Fragments arranged by size:
1753 958 881 753 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 3980 1239
Size: 1013 1728 2579
Fragments arranged by size:
2579 1728 1013
Bpu10I CC'TnA_GC
Cuts at: 2581 2581
Size: 5320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3445 4310 4608 3445
Size: 865 298 4157
Fragments arranged by size:
4157 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3983 882
Size: 617 2484 2219
Fragments arranged by size:
2484 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3111 493
Size: 711 1907 2702
Fragments arranged by size:
2702 1907 711
BsaBI GATnn'nnATC
Cuts at: 5314 5314
Size: 5320
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 3127 3509 4144
Size: 53 83 186 936 792 802 382 635
Cuts at: 4144 275
Size: 1451
Fragments arranged by size:
1451 936 802 792 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3753 4151 4425 4572 4983 3753
Size: 398 274 147 411 4090
Fragments arranged by size:
4090 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 1178
Size: 30 165 30 822 30 4243
Fragments arranged by size:
4243 822 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 1186
Size: 1291 4029
BseYI C'CCAG_C
Cuts at: 2563 4523 5019 2563
Size: 1960 496 2864
Fragments arranged by size:
2864 1960 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4747
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2359 2770 3531 4559 1540
Size: 275 186 358 411 761 1028 2301
Fragments arranged by size:
2301 1028 761 411 358 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 3381 3466 4537 2136
Size: 1245 85 1071 2919
Fragments arranged by size:
2919 1245 1071 85
BsmI GAATG_Cn'
Cuts at: 2831 2924 2831
Size: 93 5227
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2519 2579 2639 3381 3466 4537
Size: 399 346 383 60 60 742 85 1071
Cuts at: 4537 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 383 346 85 60
60
BspEI T'CCGG_A
Cuts at: 4983 4983
Size: 5320
BspHI T'CATG_A
Cuts at: 3209 4939 3209
Size: 1730 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2675 844
Size: 1831 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3207 1394
Size: 429 1384 3507
Fragments arranged by size:
3507 1384 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3815 3989 65
Size: 1183 2567 174 1396
Fragments arranged by size:
2567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 3964 4151 1700
Size: 110 234 1920 187 2869
Fragments arranged by size:
2869 1920 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5320
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5320
BssSI C'ACGA_G
Cuts at: 3380 4392 3380
Size: 1012 4308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5320
BstBI TT'CG_AA
Cuts at: 4124 4124
Size: 5320
BstEII G'GTnAC_C
Cuts at: 1184 2170 1184
Size: 986 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 2312 2312
Size: 5320
BstYI r'GATC_y
Cuts at: 1972 2650 3017 3402 3419 4871 4957 4969 5315
Size: 678 367 385 17 1452 86 12 346
Cuts at: 5315 1972
Size: 1977
Fragments arranged by size:
1977 1452 678 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4768
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4581
Fragments arranged by size:
4581 683 56
BtsI GCAGTG_nn'
Cuts at: 2225 2836 3630 3650 2225
Size: 611 794 20 3895
Fragments arranged by size:
3895 794 611 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5285
DraI TTT'AAA
Cuts at: 2624 2624
Size: 5320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4327 809
Size: 507 3011 1802
Fragments arranged by size:
3011 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 2767 3656
Size: 54 1425 50 275 18 526 411 889
Cuts at: 3656 5285 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 526 411 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2356 2767 1537
Size: 275 544 411 4090
Fragments arranged by size:
4090 544 411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2141 2454 2589 3250 1353
Size: 720 68 313 135 661 3423
Fragments arranged by size:
3423 720 661 313 135 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3907 4281 4427 387
Size: 1064 138 2318 374 146 1280
Fragments arranged by size:
2318 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2134 2134
Size: 5320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2252 3383 3980 4767 5035 1239
Size: 393 602 18 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 787 602 597 393 268 18
EcoO109I rG'GnC_Cy
Cuts at: 2257 2576 2257
Size: 319 5001
EcoRI G'AATT_C
Cuts at: 2629 3007 2629
Size: 378 4942
FspI TGC'GCA
Cuts at: 3826 3826
Size: 5320
HaeII r_GCGC'y
Cuts at: 1678 2129 2328 2570 4467 1678
Size: 451 199 242 1897 2531
Fragments arranged by size:
2531 1897 451 242 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 2453 2485 2600
Size: 32 135 32 845 32 4 32 115
Cuts at: 2600 2632 3961 3993 4035 4067 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 845 135 115 42 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2664 2910 669
Size: 988 281 726 246 3079
Fragments arranged by size:
3079 988 726 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5320
HpaI GTT'AAC
Cuts at: 2910 2910
Size: 5320
KasI G'GCGC_C
Cuts at: 2324 2324
Size: 5320
KpnI G_GTAC'C
Cuts at: 2645 2645
Size: 5320
MfeI C'AATT_G
Cuts at: 2919 2919
Size: 5320
MluI A'CGCG_T
Cuts at: 5196 5196
Size: 5320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4434 4618 1305
Size: 3129 184 2007
Fragments arranged by size:
3129 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3498
Fragments arranged by size:
3498 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2310 3278 3637 3796 518
Size: 648 1144 968 359 159 2042
Fragments arranged by size:
2042 1144 968 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2352 2736 3413 4561 4806 1606
Size: 746 384 677 1148 245 2120
Fragments arranged by size:
2120 1148 746 677 384 245
NaeI GCC'GGC
Cuts at: 2046 2046
Size: 5320
NarI GG'CG_CC
Cuts at: 2325 2325
Size: 5320
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4768
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5320
NgoMIV G'CCGG_C
Cuts at: 2044 2044
Size: 5320
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5320
NotI GC'GGCC_GC
Cuts at: 2767 2767
Size: 5320
NspI r_CATG'y
Cuts at: 1132 1229 2284 2678 4223 1132
Size: 97 1055 394 1545 2229
Fragments arranged by size:
2229 1545 1055 394 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5320
PciI A'CATG_T
Cuts at: 4219 4219
Size: 5320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5320
PfoI T'CCnGG_A
Cuts at: 2405 2405
Size: 5320
PmeI GTTT'AAAC
Cuts at: 2624 2624
Size: 5320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 4902 4934 3389
Size: 32 1481 32 3775
Fragments arranged by size:
3775 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2257 2257
Size: 5320
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5320
PsiI TTA'TAA
Cuts at: 2890 2890
Size: 5320
PspOMI G'GGCC_C
Cuts at: 2575 2575
Size: 5320
PspXI vC'TCGA_Gb
Cuts at: 2129 2129
Size: 5320
PstI C_TGCA'G
Cuts at: 830 2672 830
Size: 1842 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5320
SacI G_AGCT'C
Cuts at: 2136 2136
Size: 5320
SalI G'TCGA_C
Cuts at: 1936 2662 1936
Size: 726 4594
SapI GCTCTTCn'nnn_
Cuts at: 2141 2141
Size: 5320
SbfI CC_TGCA'GG
Cuts at: 2672 2672
Size: 5320
ScaI AGT'ACT
Cuts at: 3568 5280 3568
Size: 1712 3608
SexAI A'CCwGG_T
Cuts at: 2259 2259
Size: 5320
SfcI C'TryA_G
Cuts at: 826 1045 2668 3803 4484 4675 826
Size: 219 1623 1135 681 191 1471
Fragments arranged by size:
1623 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2326 2326
Size: 5320
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
SgrAI Cr'CCGG_yG
Cuts at: 1700 2044 1700
Size: 344 4976
SmaI CCC'GGG
Cuts at: 1872 2123 2574 2647 1872
Size: 251 451 73 4545
Fragments arranged by size:
4545 451 251 73
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 3424 4325 4587 820
Size: 197 926 186 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3111 493
Size: 2618 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5320
SphI G_CATG'C
Cuts at: 1132 2678 1132
Size: 1546 3774
SspI AAT'ATT
Cuts at: 5 52 3244 5
Size: 47 3192 2081
Fragments arranged by size:
3192 2081 47
StuI AGG'CCT
Cuts at: 1517 2162 1517
Size: 645 4675
StyI C'CwwG_G
Cuts at: 513 1065 2759 513
Size: 552 1694 3074
Fragments arranged by size:
3074 1694 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3347 3364 3517 3702 4147 325
Size: 1255 1767 17 153 185 445 1498
Fragments arranged by size:
1767 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 3566 5278
Size: 274 80 33 51 1443 48 1541 1712
Cuts at: 5278 96
Size: 138
Fragments arranged by size:
1712 1541 1443 274 138 80 51 48
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2353 2385 5280 5312 2353
Size: 32 2895 32 2361
Fragments arranged by size:
2895 2361 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2176 1316
Size: 860 4460
XbaI T'CTAG_A
Cuts at: 2656 2656
Size: 5320
XhoI C'TCGA_G
Cuts at: 1943 2129 1943
Size: 186 5134
XmaI C'CCGG_G
Cuts at: 1870 2121 2572 2645 1870
Size: 251 451 73 4545
Fragments arranged by size:
4545 451 251 73
XmnI GAAnn'nnTTC
Cuts at: 3449 3449
Size: 5320
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3128 276
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PsrI PvuII
RsrII SacII SanDI SfiI SrfI SwaI XcmI