Restriction map for
pFN21AB7570
(Circular) (Six-base) MAPSORT of: pFN21AB7570.seq Check: 835 from: 1 to: 5542
pFN21AB7570 5542 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2521 2521
Size: 5542
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3352 278
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 1937
Size: 948 4594
Acc65I G'GTAC_C
Cuts at: 2863 2863
Size: 5542
AclI AA'CG_TT
Cuts at: 1563 3669 4042 1563
Size: 2106 373 3063
Fragments arranged by size:
3063 2106 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2685 3605 4989 5257 1632
Size: 1053 920 1384 268 1917
Fragments arranged by size:
1917 1384 1053 920 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5345
AflIII A'CryG_T
Cuts at: 1203 4441 5418 1203
Size: 3238 977 1327
Fragments arranged by size:
3238 1327 977
AgeI A'CCGG_T
Cuts at: 4373 4373
Size: 5542
AhdI GACnn_n'nnGTC
Cuts at: 4271 4271
Size: 5542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2314 1184
Size: 1130 4412
AlwNI CAG_nnn'CTG
Cuts at: 2293 2512 3285 4857 2293
Size: 219 773 1572 2978
Fragments arranged by size:
2978 1572 773 219
ApaLI G'TGCA_C
Cuts at: 3599 4755 3599
Size: 1156 4386
ApoI r'AATT_y
Cuts at: 1493 2456 2851 3077 3229 5338 1493
Size: 963 395 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 395 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4096 160
Size: 872 3064 1606
Fragments arranged by size:
3064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
AvaI C'yCGr_G
Cuts at: 1870 1943 2275 2867 1870
Size: 73 332 592 4545
Fragments arranged by size:
4545 592 332 73
BamHI G'GATC_C
Cuts at: 2872 3239 2872
Size: 367 5175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2863 4318 618
Size: 325 217 627 1076 1455 1842
Fragments arranged by size:
1842 1455 1076 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2195 2712 2733 2861 1391
Size: 645 159 517 21 128 4072
Fragments arranged by size:
4072 645 517 159 128 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2543 928
Size: 971 644 3927
Fragments arranged by size:
3927 971 644
BbvCI CC'TCA_GC
Cuts at: 2024 2328 2024
Size: 304 5238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3733 3767 1903
Size: 34 50 34 1712 34 3678
Fragments arranged by size:
3678 1712 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3434 4644 3434
Size: 1210 4332
BclI T'GATC_A
Cuts at: 1548 2229 1548
Size: 681 4861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4153 136
Size: 107 122 71 631 605 2481 1525
Fragments arranged by size:
2481 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5537 5537
Size: 5542
BlpI GC'TnA_GC
Cuts at: 2030 2959 2030
Size: 929 4613
Bme1580I G_kGCm'C
Cuts at: 1790 2150 3603 4759 1790
Size: 360 1453 1156 2573
Fragments arranged by size:
2573 1453 1156 360
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3273 4231 442
Size: 416 53 83 399 24 1856 958 1753
Fragments arranged by size:
1856 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5542
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2338 2113
Size: 225 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2726 4202 1239
Size: 1229 258 1476 2579
Fragments arranged by size:
2579 1476 1229 258
Bpu10I CC'TnA_GC
Cuts at: 2024 2328 2024
Size: 304 5238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3667 4532 4830 3667
Size: 865 298 4379
Fragments arranged by size:
4379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 2326 4205 882
Size: 617 600 227 1879 2219
Fragments arranged by size:
2219 1879 617 600 227
BsaAI yAC'GTr
Cuts at: 493 1204 2759 3333 493
Size: 711 1555 574 2702
Fragments arranged by size:
2702 1555 711 574
BsaBI GATnn'nnATC
Cuts at: 5536 5536
Size: 5542
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3349 3731 4366 275
Size: 53 83 186 936 1816 382 635 1451
Fragments arranged by size:
1816 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3975 4373 4647 4794 5205 3975
Size: 398 274 147 411 4312
Fragments arranged by size:
4312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5317
Fragments arranged by size:
5317 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2741 1186
Size: 1024 531 3987
Fragments arranged by size:
3987 1024 531
BseYI C'CCAG_C
Cuts at: 4745 5241 4745
Size: 496 5046
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4969
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2992 3753 4781 1540
Size: 275 186 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 2733 3603 3688 4759 2036
Size: 697 870 85 1071 2819
Fragments arranged by size:
2819 1071 870 697 85
BsmI GAATG_Cn'
Cuts at: 2179 3053 3146 2179
Size: 874 93 4575
Fragments arranged by size:
4575 874 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2363 2363
Size: 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2150 2195 2712 2733 2861 3603
Size: 399 246 114 45 517 21 128 742
Cuts at: 3603 3688 4759 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 246 128 114
85 45 21
BspEI T'CCGG_A
Cuts at: 5205 5205
Size: 5542
BspHI T'CATG_A
Cuts at: 2079 3431 5161 2079
Size: 1352 1730 2460
Fragments arranged by size:
2460 1730 1352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2521 2897 844
Size: 1273 404 376 3489
Fragments arranged by size:
3489 1273 404 376
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 3429 1394
Size: 429 395 1211 3507
Fragments arranged by size:
3507 1211 429 395
BsrDI GCAATG_nn'
Cuts at: 65 1248 4037 4211 65
Size: 1183 2789 174 1396
Fragments arranged by size:
2789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4186 4373 1700
Size: 110 2376 187 2869
Fragments arranged by size:
2869 2376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5542
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5542
BssSI C'ACGA_G
Cuts at: 3602 4614 3602
Size: 1012 4530
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5542
BstBI TT'CG_AA
Cuts at: 4346 4346
Size: 5542
BstEII G'GTnAC_C
Cuts at: 1184 2308 1184
Size: 1124 4418
BstYI r'GATC_y
Cuts at: 1972 2091 2872 3239 3624 3641 5093 5179 5191
Size: 119 781 367 385 17 1452 86 12
Cuts at: 5191 5537 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 781 385 367 346 119 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4990
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4803
Fragments arranged by size:
4803 683 56
BtsI GCAGTG_nn'
Cuts at: 2008 3058 3852 3872 2008
Size: 1050 794 20 3678
Fragments arranged by size:
3678 1050 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5507
DraI TTT'AAA
Cuts at: 2846 2846
Size: 5542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4549 809
Size: 507 3233 1802
Fragments arranged by size:
3233 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2989 3878
Size: 54 1425 50 275 18 211 948 889
Cuts at: 3878 5507 8
Size: 1629 43
Fragments arranged by size:
1629 1425 948 889 275 211 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2989 1537
Size: 275 1177 4090
Fragments arranged by size:
4090 1177 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 2661 2824 3472 1353
Size: 1293 15 163 648 3423
Fragments arranged by size:
3423 1293 648 163 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4129 4503 4649 387
Size: 1064 138 2540 374 146 1280
Fragments arranged by size:
2540 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2034 2731 2034
Size: 697 4845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2685 2726 3605 4202 4989 5257
Size: 393 836 217 41 879 597 787 268
Cuts at: 5257 1239
Size: 1524
Fragments arranged by size:
1524 879 836 787 597 393 268 217
41
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2099
Size: 5542
EcoO109I rG'GnC_Cy
Cuts at: 2332 2347 2332
Size: 15 5527
EcoRI G'AATT_C
Cuts at: 2851 3229 2851
Size: 378 5164
FspI TGC'GCA
Cuts at: 4048 4048
Size: 5542
HaeII r_GCGC'y
Cuts at: 1678 2068 4689 1678
Size: 390 2621 2531
Fragments arranged by size:
2621 2531 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2530 2562 4183 4215 4257
Size: 32 135 32 958 32 1621 32 42
Cuts at: 4257 4289 1373
Size: 32 2626
Fragments arranged by size:
2626 1621 958 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 2886 3132 669
Size: 988 281 543 405 246 3079
Fragments arranged by size:
3079 988 543 405 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5542
HpaI GTT'AAC
Cuts at: 3132 3132
Size: 5542
KpnI G_GTAC'C
Cuts at: 2867 2867
Size: 5542
MfeI C'AATT_G
Cuts at: 3141 3141
Size: 5542
MluI A'CGCG_T
Cuts at: 5418 5418
Size: 5542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 4656 4840 1305
Size: 1170 2181 184 2007
Fragments arranged by size:
2181 2007 1170 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3720
Fragments arranged by size:
3720 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3500 3859 4018 518
Size: 648 2334 359 159 2042
Fragments arranged by size:
2334 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2704 2958 3635 4783 5028 1606
Size: 921 177 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 254 245 177
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4990
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5542
NotI GC'GGCC_GC
Cuts at: 2989 2989
Size: 5542
NspI r_CATG'y
Cuts at: 1132 1229 2900 4445 1132
Size: 97 1671 1545 2229
Fragments arranged by size:
2229 1671 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2336 2689 1348
Size: 988 353 4201
Fragments arranged by size:
4201 988 353
PciI A'CATG_T
Cuts at: 4441 4441
Size: 5542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5542
PmeI GTTT'AAAC
Cuts at: 2846 2846
Size: 5542
PmlI CAC'GTG
Cuts at: 2759 2759
Size: 5542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 5124 5156 3611
Size: 32 1481 32 3997
Fragments arranged by size:
3997 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5542
PsiI TTA'TAA
Cuts at: 3112 3112
Size: 5542
PstI C_TGCA'G
Cuts at: 830 2436 2532 2894 830
Size: 1606 96 362 3478
Fragments arranged by size:
3478 1606 362 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5542
PvuII CAG'CTG
Cuts at: 2527 2704 2527
Size: 177 5365
SacI G_AGCT'C
Cuts at: 2036 2733 2036
Size: 697 4845
SalI G'TCGA_C
Cuts at: 1936 2884 1936
Size: 948 4594
SbfI CC_TGCA'GG
Cuts at: 2894 2894
Size: 5542
ScaI AGT'ACT
Cuts at: 3790 5502 3790
Size: 1712 3830
SexAI A'CCwGG_T
Cuts at: 2259 2304 2540 2259
Size: 45 236 5261
Fragments arranged by size:
5261 236 45
SfcI C'TryA_G
Cuts at: 826 1045 2432 2528 2890 4025 4706 4897 826
Size: 219 1387 96 362 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 681 362 219 191 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5542
SmaI CCC'GGG
Cuts at: 1872 2277 2869 1872
Size: 405 592 4545
Fragments arranged by size:
4545 592 405
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3646 4547 4809 820
Size: 197 926 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3333 493
Size: 2840 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5542
SphI G_CATG'C
Cuts at: 1132 2900 1132
Size: 1768 3774
SspI AAT'ATT
Cuts at: 5 52 3466 5
Size: 47 3414 2081
Fragments arranged by size:
3414 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5542
StyI C'CwwG_G
Cuts at: 513 1065 2100 2981 513
Size: 552 1035 881 3074
Fragments arranged by size:
3074 1035 881 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 3569 3586 3739 3924 4369 325
Size: 1255 1037 952 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1037 952 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3788 5500 96
Size: 274 80 33 51 1443 1811 1712 138
Fragments arranged by size:
1811 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2748 2780 5502 5534 2748
Size: 32 2722 32 2756
Fragments arranged by size:
2756 2722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5542
XbaI T'CTAG_A
Cuts at: 2878 2878
Size: 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5542
XmaI C'CCGG_G
Cuts at: 1870 2275 2867 1870
Size: 405 592 4545
Fragments arranged by size:
4545 592 405
XmnI GAAnn'nnTTC
Cuts at: 2456 3671 2456
Size: 1215 4327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3350 276
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI