Restriction map for
pFN21AB7560
(Circular) (Six-base) MAPSORT of: pFN21AB7560.seq Check: 6658 from: 1 to: 5521
pFN21AB7560 5521 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2220 2842 2220
Size: 622 4899
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3584 4968 5236 1632
Size: 1952 1384 268 1917
Fragments arranged by size:
1952 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2175 1184
Size: 991 4530
AlwNI CAG_nnn'CTG
Cuts at: 2551 3264 4836 2551
Size: 713 1572 3236
Fragments arranged by size:
3236 1572 713
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2356 2600 2846 1870
Size: 73 243 170 244 246 4545
Fragments arranged by size:
4545 246 244 243 170 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2101 2220 2381 2842
Size: 325 217 627 255 59 119 161 461
Cuts at: 2842 4297 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 461 325 255 217 161
119 59
BanII G_rGCy'C
Cuts at: 1391 2840 1391
Size: 1449 4072
BbeI G_GCGC'C
Cuts at: 2046 2105 2385 2046
Size: 59 280 5182
Fragments arranged by size:
5182 280 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2591 2625 3712 3746 1903
Size: 34 654 34 1087 34 3678
Fragments arranged by size:
3678 1087 654 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2114 4132
Size: 107 122 71 631 605 337 105 2018
Cuts at: 4132 136
Size: 1525
Fragments arranged by size:
2018 1525 631 605 337 122 107 105
71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2061 3582 4738 1790
Size: 271 1521 1156 2573
Fragments arranged by size:
2573 1521 1156 271
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4181 1239
Size: 2942 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2441 4184 882
Size: 617 942 1743 2219
Fragments arranged by size:
2219 1743 942 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2102 2382 3328
Size: 53 83 186 936 510 59 280 946
Cuts at: 3328 3710 4345 275
Size: 382 635 1451
Fragments arranged by size:
1451 946 936 635 510 382 280 186
83 59 53
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2609 2639 1178
Size: 30 165 30 1206 30 4060
Fragments arranged by size:
4060 1206 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2360 1186
Size: 1081 93 4347
Fragments arranged by size:
4347 1081 93
BseYI C'CCAG_C
Cuts at: 2162 2258 4724 5220 2162
Size: 96 2466 496 2463
Fragments arranged by size:
2466 2463 496 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2410 2631 1163
Size: 573 674 221 4053
Fragments arranged by size:
4053 674 573 221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2971 3732 4760 1540
Size: 275 186 120 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186 120
BsiHKAI G_wGCw'C
Cuts at: 3582 3667 4738 3582
Size: 85 1071 4365
Fragments arranged by size:
4365 1071 85
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2840 3582 3667 4738 1391
Size: 399 271 779 742 85 1071 2174
Fragments arranged by size:
2174 1071 779 742 399 271 85
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 2876 844
Size: 1588 444 3489
Fragments arranged by size:
3489 1588 444
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4165 4352 1700
Size: 110 2355 187 2869
Fragments arranged by size:
2869 2355 187 110
BsrGI T'GTAC_A
Cuts at: 96 2511 96
Size: 2415 3106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 2452 3581 4593 2452
Size: 1129 1012 3380
Fragments arranged by size:
3380 1129 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2424 794
Size: 1630 3891
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2332 2648 2851 3218 3603 3620 5072 5158
Size: 360 316 203 367 385 17 1452 86
Cuts at: 5158 5170 5516 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 360 346 316 203
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 2121 2480 513
Size: 552 939 63 54 359 3554
Fragments arranged by size:
3554 939 552 359 63 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 3831 3851 3037
Size: 794 20 4707
Fragments arranged by size:
4707 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2463 2968
Size: 54 1425 50 275 18 288 345 505
Cuts at: 2968 3857 5486 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 505 345 288 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2968 1537
Size: 275 306 850 4090
Fragments arranged by size:
4090 850 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 2531 3451 1353
Size: 891 287 920 3423
Fragments arranged by size:
3423 920 891 287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 4108 4482 4628 387
Size: 1064 138 699 1820 374 146 1280
Fragments arranged by size:
1820 1280 1064 699 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3584 4181 4968 5236 1239
Size: 393 1952 597 787 268 1524
Fragments arranged by size:
1952 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2548 2548
Size: 5521
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2046 2105 2385 4668 1678
Size: 368 59 280 2283 2531
Fragments arranged by size:
2531 2283 368 280 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4162 4194 4236 4268 1373
Size: 32 135 32 2590 32 42 32 2626
Fragments arranged by size:
2626 2590 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KasI G'GCGC_C
Cuts at: 2042 2101 2381 2042
Size: 59 280 5182
Fragments arranged by size:
5182 280 59
KpnI G_GTAC'C
Cuts at: 2224 2846 2224
Size: 622 4899
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 10
Size: 54 1425 343 633 3066
Fragments arranged by size:
3066 1425 633 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 3838 3997 518
Size: 648 2313 359 159 2042
Fragments arranged by size:
2313 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2069 2123 2376 2400 2937 3614 4762
Size: 446 17 54 253 24 537 677 1148
Cuts at: 4762 5007 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 537 446 253 245 54
24 17
NarI GG'CG_CC
Cuts at: 2043 2102 2382 2043
Size: 59 280 5182
Fragments arranged by size:
5182 280 59
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2118 2968 2118
Size: 850 4671
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PfoI T'CCnGG_A
Cuts at: 2806 2806
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PspXI vC'TCGA_Gb
Cuts at: 2600 2600
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2444 2873 830
Size: 1614 429 3478
Fragments arranged by size:
3478 1614 429
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
SacII CC_GC'GG
Cuts at: 2070 2124 2070
Size: 54 5467
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SapI GCTCTTCn'nnn_
Cuts at: 2531 2531
Size: 5521
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2440 2869 4004 4685 4876 826
Size: 219 1395 429 1135 681 191 1471
Fragments arranged by size:
1471 1395 1135 681 429 219 191
SfoI GGC'GCC
Cuts at: 2044 2103 2383 2044
Size: 59 280 5182
Fragments arranged by size:
5182 280 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2188 2848 1872
Size: 316 660 4545
Fragments arranged by size:
4545 660 316
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 3625 4526 4788 820
Size: 197 926 657 1025 901 262 1553
Fragments arranged by size:
1553 1025 926 901 657 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2960 513
Size: 552 939 93 863 3074
Fragments arranged by size:
3074 939 863 552 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 3767 5479
Size: 274 80 33 51 1443 534 1256 1712
Cuts at: 5479 96
Size: 138
Fragments arranged by size:
1712 1443 1256 534 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2335 2857 2335
Size: 522 4999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 2458
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 2600 1943
Size: 657 4864
XmaI C'CCGG_G
Cuts at: 1870 2186 2846 1870
Size: 316 660 4545
Fragments arranged by size:
4545 660 316
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII
RsrII SacI SanDI SexAI SfiI SrfI SwaI