Restriction map for
pFN21AB7559
(Circular) (Six-base) MAPSORT of: pFN21AB7559.seq Check: 6256 from: 1 to: 5323
pFN21AB7559 5323 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 2487 2243
Size: 244 5079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3133 278
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 1937
Size: 729 4594
Acc65I G'GTAC_C
Cuts at: 2644 2644
Size: 5323
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3386 4770 5038 1632
Size: 1754 1384 268 1917
Fragments arranged by size:
1917 1754 1384 268
AfeI AGC'GCT
Cuts at: 2177 2177
Size: 5323
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 4222 5199 1203
Size: 3019 977 1327
Fragments arranged by size:
3019 1327 977
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 2137 4052 2137
Size: 1915 3408
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5323
AlwNI CAG_nnn'CTG
Cuts at: 3066 4638 3066
Size: 1572 3751
ApaLI G'TGCA_C
Cuts at: 2522 3380 4536 2522
Size: 858 1156 3309
Fragments arranged by size:
3309 1156 858
ApoI r'AATT_y
Cuts at: 1493 2632 2858 3010 5119 1493
Size: 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2557 2648 1870
Size: 73 614 91 4545
Fragments arranged by size:
4545 614 91 73
BamHI G'GATC_C
Cuts at: 2653 3020 2653
Size: 367 4956
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2413 2644 4099 618
Size: 325 217 627 626 231 1455 1842
Fragments arranged by size:
1842 1455 627 626 325 231 217
BanII G_rGCy'C
Cuts at: 1391 2018 2303 2642 1391
Size: 627 285 339 4072
Fragments arranged by size:
4072 627 339 285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2569 928
Size: 971 670 3682
Fragments arranged by size:
3682 971 670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3514 3548 1903
Size: 34 1577 34 3678
Fragments arranged by size:
3678 1577 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4425 3215
Size: 1210 4113
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3934 136
Size: 107 122 71 631 605 2262 1525
Fragments arranged by size:
2262 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5318 5318
Size: 5323
BlpI GC'TnA_GC
Cuts at: 2302 2372 2740 2302
Size: 70 368 4885
Fragments arranged by size:
4885 368 70
Bme1580I G_kGCm'C
Cuts at: 1790 2192 2359 2418 2526 3384 4540 1790
Size: 402 167 59 108 858 1156 2573
Fragments arranged by size:
2573 1156 858 402 167 108 59
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5323
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 2522 3054
Size: 416 53 83 399 24 704 401 532
Cuts at: 3054 4012 442
Size: 958 1753
Fragments arranged by size:
1753 958 704 532 416 401 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2371 3983 1239
Size: 1132 1612 2579
Fragments arranged by size:
2579 1612 1132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 3448 4313 4611 2378
Size: 1070 865 298 3090
Fragments arranged by size:
3090 1070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2560 3986 882
Size: 617 1061 1426 2219
Fragments arranged by size:
2219 1426 1061 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 3114 493
Size: 711 1317 593 2702
Fragments arranged by size:
2702 1317 711 593
BsaBI GATnn'nnATC
Cuts at: 5317 5317
Size: 5323
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3130 3512 4147 275
Size: 53 83 186 936 1597 382 635 1451
Fragments arranged by size:
1597 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2029 2566 3756 4154 4428 4575 4986 2029
Size: 537 1190 398 274 147 411 2366
Fragments arranged by size:
2366 1190 537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5098
Fragments arranged by size:
5098 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2162 2165 2183 2186 1186
Size: 862 114 3 18 3 4323
Fragments arranged by size:
4323 862 114 18 3 3
BseYI C'CCAG_C
Cuts at: 2237 4526 5022 2237
Size: 2289 496 2538
Fragments arranged by size:
2538 2289 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4750
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2773 3534 4562 1540
Size: 275 186 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2526 3384 3469 4540 2526
Size: 858 85 1071 3309
Fragments arranged by size:
3309 1071 858 85
BsmI GAATG_Cn'
Cuts at: 2834 2927 2834
Size: 93 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2192 2303 2359 2418 2526 2642
Size: 399 228 174 111 56 59 108 116
Cuts at: 2642 3384 3469 4540 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 228 174 116 111
108 85 59 56
BspEI T'CCGG_A
Cuts at: 2029 4986 2029
Size: 2957 2366
BspHI T'CATG_A
Cuts at: 3212 4942 3212
Size: 1730 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 2487 2678 844
Size: 1399 244 191 3489
Fragments arranged by size:
3489 1399 244 191
BsrBI CCG'CTC
Cuts at: 1394 1823 3210 1394
Size: 429 1387 3507
Fragments arranged by size:
3507 1387 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 3992 65
Size: 1183 2570 174 1396
Fragments arranged by size:
2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2195 3967 4154 1700
Size: 110 385 1772 187 2869
Fragments arranged by size:
2869 1772 385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5323
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5323
BssSI C'ACGA_G
Cuts at: 3383 4395 3383
Size: 1012 4311
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2491 794
Size: 1697 3626
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5323
BstYI r'GATC_y
Cuts at: 1972 2653 3020 3405 3422 4874 4960 4972 5318
Size: 681 367 385 17 1452 86 12 346
Cuts at: 5318 1972
Size: 1977
Fragments arranged by size:
1977 1452 681 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2010 2098 513
Size: 552 945 88 3738
Fragments arranged by size:
3738 945 552 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 487
Size: 683 56 1229 3355
Fragments arranged by size:
3355 1229 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 3633 3653 2839
Size: 794 20 4509
Fragments arranged by size:
4509 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5288
DraI TTT'AAA
Cuts at: 2627 2627
Size: 5323
DraIII CAC_nnn'GTG
Cuts at: 2530 2530
Size: 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4330 809
Size: 507 3014 1802
Fragments arranged by size:
3014 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 2337 2543
Size: 54 1425 50 275 18 462 45 206
Cuts at: 2543 2770 3659 5288 8
Size: 227 889 1629 43
Fragments arranged by size:
1629 1425 889 462 275 227 206 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2770 1537
Size: 275 958 4090
Fragments arranged by size:
4090 958 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3253 1353
Size: 1900 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4284 4430 387
Size: 1064 138 2321 374 146 1280
Fragments arranged by size:
2321 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 3386 3983 4770 5038 1239
Size: 393 739 1015 597 787 268 1524
Fragments arranged by size:
1524 1015 787 739 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2083 2386 2083
Size: 303 5020
EcoO109I rG'GnC_Cy
Cuts at: 2155 2155
Size: 5323
EcoRI G'AATT_C
Cuts at: 2632 3010 2632
Size: 378 4945
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 2179 4470 1678
Size: 501 2291 2531
Fragments arranged by size:
2531 2291 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 3964 3996 4038
Size: 32 135 32 551 32 1809 32 42
Cuts at: 4038 4070 1373
Size: 32 2626
Fragments arranged by size:
2626 1809 551 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2667 2913 669
Size: 988 281 729 246 3079
Fragments arranged by size:
3079 988 729 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5323
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KpnI G_GTAC'C
Cuts at: 2648 2648
Size: 5323
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4437 4621 1305
Size: 3132 184 2007
Fragments arranged by size:
3132 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2545 10
Size: 54 1425 343 713 2788
Fragments arranged by size:
2788 1425 713 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3281 3640 3799 518
Size: 648 2115 359 159 2042
Fragments arranged by size:
2115 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2100 2329 2488 2621 2739 3416 4564
Size: 453 41 229 159 133 118 677 1148
Cuts at: 4564 4809 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 453 245 229 159 133
118 41
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 5323
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4771
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2681 4226 1132
Size: 97 1452 1545 2229
Fragments arranged by size:
2229 1545 1452 97
PasI CC'CwG_GG
Cuts at: 1348 2409 1348
Size: 1061 4262
PciI A'CATG_T
Cuts at: 4222 4222
Size: 5323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 4905 4937 3392
Size: 32 1481 32 3778
Fragments arranged by size:
3778 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2155
Size: 5323
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2058 2675 830
Size: 1228 617 3478
Fragments arranged by size:
3478 1228 617
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
PvuII CAG'CTG
Cuts at: 2488 2488
Size: 5323
SacII CC_GC'GG
Cuts at: 2101 2101
Size: 5323
SalI G'TCGA_C
Cuts at: 1936 2665 1936
Size: 729 4594
SbfI CC_TGCA'GG
Cuts at: 2675 2675
Size: 5323
ScaI AGT'ACT
Cuts at: 3571 5283 3571
Size: 1712 3611
SfcI C'TryA_G
Cuts at: 826 1045 2054 2671 3806 4487 4678 826
Size: 219 1009 617 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 617 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5323
SmaI CCC'GGG
Cuts at: 1872 2650 1872
Size: 778 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3427 4328 4590 820
Size: 197 926 450 1034 901 262 1553
Fragments arranged by size:
1553 1034 926 901 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5323
SphI G_CATG'C
Cuts at: 1132 2681 1132
Size: 1549 3774
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5323
StyI C'CwwG_G
Cuts at: 513 1065 2295 2762 513
Size: 552 1230 467 3074
Fragments arranged by size:
3074 1230 552 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2401 2541 3350 3367 3520 3705 4150
Size: 1255 821 140 809 17 153 185 445
Cuts at: 4150 325
Size: 1498
Fragments arranged by size:
1498 1255 821 809 445 185 153 140
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 5281 96
Size: 274 80 33 51 1443 1592 1712 138
Fragments arranged by size:
1712 1592 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5283 5315 5283
Size: 32 5291
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5323
XbaI T'CTAG_A
Cuts at: 2659 2659
Size: 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2648 1870
Size: 778 4545
XmnI GAAnn'nnTTC
Cuts at: 3452 3452
Size: 5323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3131 276
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI