Restriction map for
pFN21AB7557
(Circular) (Six-base) MAPSORT of: pFN21AB7557.seq Check: 5673 from: 1 to: 5311
pFN21AB7557 5311 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2564 2654 1937
Size: 627 90 4594
Fragments arranged by size:
4594 627 90
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 2150 3438 3811 1563
Size: 587 1288 373 3063
Fragments arranged by size:
3063 1288 587 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2418 3374 4758 5026 1632
Size: 464 322 956 1384 268 1917
Fragments arranged by size:
1917 1384 956 464 322 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 2054 1166
Size: 888 4423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2427 1184
Size: 1243 4068
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaI G_GGCC'C
Cuts at: 2492 2492
Size: 5311
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2351 2620 2846 2998 5107 1493
Size: 858 269 226 152 2109 1697
Fragments arranged by size:
2109 1697 858 269 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2636 1870
Size: 73 693 4545
Fragments arranged by size:
4545 693 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2632 4087 618
Size: 325 217 627 261 584 1455 1842
Fragments arranged by size:
1842 1455 627 584 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2492 2630 1391
Size: 1101 138 4072
Fragments arranged by size:
4072 1101 138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2297 928
Size: 971 398 3942
Fragments arranged by size:
3942 971 398
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2134 2168 3502 3536 1903
Size: 34 197 34 1334 34 3678
Fragments arranged by size:
3678 1334 197 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3922 136
Size: 107 122 71 631 605 2250 1525
Fragments arranged by size:
2250 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2117 5306 2117
Size: 3189 2122
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2492 3372 4528 1790
Size: 702 880 1156 2573
Fragments arranged by size:
2573 1156 880 702
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5311
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 4000 442
Size: 416 53 83 399 24 1625 958 1753
Fragments arranged by size:
1753 1625 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 2456
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3971 1239
Size: 2732 2579
Bpu10I CC'TnA_GC
Cuts at: 2064 2064
Size: 5311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2106 2446 3436 4301 4599 2106
Size: 340 990 865 298 2818
Fragments arranged by size:
2818 990 865 340 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3974 882
Size: 617 2475 2219
Fragments arranged by size:
2475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 2334 5305 2334
Size: 2971 2340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3118 3500 4135 275
Size: 53 83 186 936 1585 382 635 1451
Fragments arranged by size:
1585 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 1186
Size: 1285 4026
BseYI C'CCAG_C
Cuts at: 2080 2485 4514 5010 2080
Size: 405 2029 496 2381
Fragments arranged by size:
2381 2029 496 405
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4738
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3522 4550 1540
Size: 275 186 760 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 3372 3457 4528 2406
Size: 966 85 1071 3189
Fragments arranged by size:
3189 1071 966 85
BsmI GAATG_Cn'
Cuts at: 2556 2822 2915 2556
Size: 266 93 4952
Fragments arranged by size:
4952 266 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2406 2492 2630 3372 3457 4528 1391
Size: 399 616 86 138 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 399 138 86 85
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 2442 3200 4930 2442
Size: 758 1730 2823
Fragments arranged by size:
2823 1730 758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 2666 844
Size: 1556 266 3489
Fragments arranged by size:
3489 1556 266
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3955 4142 1700
Size: 110 2145 187 2869
Fragments arranged by size:
2869 2145 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2117 2641 3008 3393 3410 4862 4948 4960
Size: 145 524 367 385 17 1452 86 12
Cuts at: 4960 5306 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 524 385 367 346 145 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2522 513
Size: 552 939 518 3302
Fragments arranged by size:
3302 939 552 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2758 3647 5276
Size: 54 1425 50 275 18 928 889 1629
Cuts at: 5276 8
Size: 43
Fragments arranged by size:
1629 1425 928 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2758 1537
Size: 275 946 4090
Fragments arranged by size:
4090 946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 3241 1353
Size: 1041 847 3423
Fragments arranged by size:
3423 1041 847
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2418 3374 3971 4758 5026 1239
Size: 393 464 322 956 597 787 268 1524
Fragments arranged by size:
1524 956 787 597 464 393 322 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2453 2453
Size: 5311
EcoO109I rG'GnC_Cy
Cuts at: 2240 2489 2240
Size: 249 5062
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 2415 3817 2415
Size: 1402 3909
HaeII r_GCGC'y
Cuts at: 1678 4458 1678
Size: 2780 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2082 2102 2114 2134 2500
Size: 32 135 32 510 20 12 20 366
Cuts at: 2500 2532 3952 3984 4026 4058 1373
Size: 32 1420 32 42 32 2626
Fragments arranged by size:
2626 1420 510 366 135 42 32 32
32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 2565 2655 2901 669
Size: 988 281 268 359 90 246 3079
Fragments arranged by size:
3079 988 359 281 268 246 90
HindIII A'AGCT_T
Cuts at: 748 2263 748
Size: 1515 3796
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 3269 3628 3787 518
Size: 648 888 1215 359 159 2042
Fragments arranged by size:
2042 1215 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2485 2727 3404 4552 4797 1606
Size: 879 242 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 245 242
NcoI C'CATG_G
Cuts at: 513 1065 2004 2522 513
Size: 552 939 518 3302
Fragments arranged by size:
3302 939 552 518
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5311
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2141 1108
Size: 1033 4278
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2240 2240
Size: 5311
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PspOMI G'GGCC_C
Cuts at: 2488 2488
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2663 830
Size: 1833 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
PvuII CAG'CTG
Cuts at: 2485 2485
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2563 2653 1936
Size: 627 90 4594
Fragments arranged by size:
4594 627 90
SanDI GG'GwC_CC
Cuts at: 2240 2240
Size: 5311
SapI GCTCTTCn'nnn_
Cuts at: 2394 2394
Size: 5311
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2214 2659 3794 4475 4666 826
Size: 219 1169 445 1135 681 191 1471
Fragments arranged by size:
1471 1169 1135 681 445 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2638 1872
Size: 766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2121 2425 3415 4316 4578 820
Size: 197 926 178 304 990 901 262 1553
Fragments arranged by size:
1553 990 926 901 304 262 197 178
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2004 2235 2522 2750 513
Size: 552 939 231 287 228 3074
Fragments arranged by size:
3074 939 552 287 231 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3338 3355 3508 3693 4138 325
Size: 1255 1758 17 153 185 445 1498
Fragments arranged by size:
1758 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2371 2647 2371
Size: 276 5035
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2636 1870
Size: 766 4545
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI