Restriction map for
pFN21AB7554
(Circular) (Six-base) MAPSORT of: pFN21AB7554.seq Check: 7900 from: 1 to: 5515
pFN21AB7554 5515 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 2646 3258 4830 2646
Size: 612 1572 3331
Fragments arranged by size:
3331 1572 612
ApaI G_GGCC'C
Cuts at: 2130 2300 2130
Size: 170 5345
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2468 2824 3050 3202 5311 1493
Size: 975 356 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 356 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2498 2840 1870
Size: 73 78 477 342 4545
Fragments arranged by size:
4545 477 342 78 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2047 2836 4291 618
Size: 325 217 627 249 11 789 1455 1842
Fragments arranged by size:
1842 1455 789 627 325 249 217 11
BanII G_rGCy'C
Cuts at: 1391 2130 2220 2300 2387 2834 1391
Size: 739 90 80 87 447 4072
Fragments arranged by size:
4072 739 447 90 87 80
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 5515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2742 928
Size: 971 843 3701
Fragments arranged by size:
3701 971 843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3706 3740 1903
Size: 34 50 34 1685 34 3678
Fragments arranged by size:
3678 1685 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 2650 2767 1548
Size: 1102 117 4296
Fragments arranged by size:
4296 1102 117
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 4126 136
Size: 107 122 71 631 605 592 1862 1525
Fragments arranged by size:
1862 1525 631 605 592 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2531 2932 2531
Size: 401 5114
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2300 3576 4732 1790
Size: 340 170 1276 1156 2573
Fragments arranged by size:
2573 1276 1156 340 170
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2664 3246 4204
Size: 416 53 83 399 24 1247 582 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 1247 958 582 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2401 2480 2832 4175 1239
Size: 1162 79 352 1343 2579
Fragments arranged by size:
2579 1343 1162 352 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 2766 5509 2766
Size: 2743 2772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2093 3322 3704
Size: 53 83 186 936 504 56 1229 382
Cuts at: 3704 4339 275
Size: 635 1451
Fragments arranged by size:
1451 1229 936 635 504 382 186 83
56 53
BsaWI w'CCGG_w
Cuts at: 2199 3948 4346 4620 4767 5178 2199
Size: 1749 398 274 147 411 2536
Fragments arranged by size:
2536 1749 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5290
Fragments arranged by size:
5290 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2596 1186
Size: 975 435 4105
Fragments arranged by size:
4105 975 435
BseYI C'CCAG_C
Cuts at: 4718 5214 4718
Size: 496 5019
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3576 3661 4732 3576
Size: 85 1071 4359
Fragments arranged by size:
4359 1071 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
BsmBI CGTCTCn'nnnn_
Cuts at: 2468 2468
Size: 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2220 2300 2387 2834 3576 3661
Size: 399 340 90 80 87 447 742 85
Cuts at: 3661 4732 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 340 90 87
85 80
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2026 4159 4346 1700
Size: 110 216 2133 187 2869
Fragments arranged by size:
2869 2133 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 2186 3575 4587 2186
Size: 1389 1012 3114
Fragments arranged by size:
3114 1389 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2344 2665 794
Size: 1550 321 3644
Fragments arranged by size:
3644 1550 321
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2458
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2040 2122 2315 513
Size: 552 975 82 193 3713
Fragments arranged by size:
3713 975 552 193 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 2422 2514
Size: 54 1425 50 275 18 435 157 92
Cuts at: 2514 2962 3851 5480 8
Size: 448 889 1629 43
Fragments arranged by size:
1629 1425 889 448 435 275 157 92
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 1353
Size: 2092 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2242 2339 2484 4102 4476 4622
Size: 1064 138 653 97 145 1618 374 146
Cuts at: 4622 387
Size: 1280
Fragments arranged by size:
1618 1280 1064 653 374 146 145 138
97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2401 2480 2832 3578 4175 4962 5230
Size: 393 769 79 352 746 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 787 769 746 597 393 352 268
79
EcoO109I rG'GnC_Cy
Cuts at: 2296 2297 2296
Size: 1 5514
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2040 4662 1678
Size: 362 2622 2531
Fragments arranged by size:
2622 2531 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2494 2526 2729 2761 4156
Size: 32 135 32 922 32 203 32 1395
Cuts at: 4156 4188 4230 4262 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1395 922 203 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2463 4629 4813 1305
Size: 1158 2166 184 2007
Fragments arranged by size:
2166 2007 1158 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2516 10
Size: 54 1425 343 684 3009
Fragments arranged by size:
3009 1425 684 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3473 3832 3991 518
Size: 648 2307 359 159 2042
Fragments arranged by size:
2307 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 2071 2124 2270 2317 2931 3608 4756
Size: 447 18 53 146 47 614 677 1148
Cuts at: 4756 5001 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 614 447 245 146 53
47 18
NaeI GCC'GGC
Cuts at: 2028 2028
Size: 5515
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4963
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NgoMIV G'CCGG_C
Cuts at: 2026 2026
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2362 1348
Size: 1014 4501
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2126 2296 2126
Size: 170 5345
PstI C_TGCA'G
Cuts at: 830 2529 2867 830
Size: 1699 338 3478
Fragments arranged by size:
3478 1699 338
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SacII CC_GC'GG
Cuts at: 2125 2318 2125
Size: 193 5322
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SexAI A'CCwGG_T
Cuts at: 2552 2552
Size: 5515
SfcI C'TryA_G
Cuts at: 826 1045 2525 2863 3998 4679 4870 826
Size: 219 1480 338 1135 681 191 1471
Fragments arranged by size:
1480 1471 1135 681 338 219 191
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2023 2842 1872
Size: 151 819 4545
Fragments arranged by size:
4545 819 151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 2401 1517
Size: 884 4631
StyI C'CwwG_G
Cuts at: 513 1065 2954 513
Size: 552 1889 3074
Fragments arranged by size:
3074 1889 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2021 2840 1870
Size: 151 819 4545
Fragments arranged by size:
4545 819 151
XmnI GAAnn'nnTTC
Cuts at: 2468 3644 2468
Size: 1176 4339
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI
SapI SfiI SrfI SwaI XcmI