Restriction map for
pFN21AB7550
(Circular) (Six-base) MAPSORT of: pFN21AB7550.seq Check: 4293 from: 1 to: 5506
pFN21AB7550 5506 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2369 2369
Size: 5506
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2433 3316 278
Size: 53 83 186 936 897 883 2468
Fragments arranged by size:
2468 936 897 883 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 2720 3569 4953 5221 1632
Size: 645 443 849 1384 268 1917
Fragments arranged by size:
1917 1384 849 645 443 268
AfeI AGC'GCT
Cuts at: 2561 2561
Size: 5506
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 2494 2613 2643 4405 5382 1203
Size: 1291 119 30 1762 977 1327
Fragments arranged by size:
1762 1327 1291 977 119 30
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 2513 1166
Size: 1347 4159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AlwNI CAG_nnn'CTG
Cuts at: 3249 4821 3249
Size: 1572 3934
ApaI G_GGCC'C
Cuts at: 2232 2232
Size: 5506
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2815 3041 3193 5302 1493
Size: 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2831 1870
Size: 73 282 606 4545
Fragments arranged by size:
4545 606 282 73
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2439 2827 4282 618
Size: 325 217 627 652 388 1455 1842
Fragments arranged by size:
1842 1455 652 627 388 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2232 2825 1391
Size: 679 162 593 4072
Fragments arranged by size:
4072 679 593 162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2265 928
Size: 971 366 4169
Fragments arranged by size:
4169 971 366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3697 3731 1903
Size: 34 50 34 1676 34 3678
Fragments arranged by size:
3678 1676 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3398 4608 3398
Size: 1210 4296
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2682 4117 136
Size: 107 122 71 631 605 1010 1435 1525
Fragments arranged by size:
1525 1435 1010 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2091 5501 2091
Size: 3410 2096
BlpI GC'TnA_GC
Cuts at: 2923 2923
Size: 5506
Bme1580I G_kGCm'C
Cuts at: 1790 2232 3567 4723 1790
Size: 442 1335 1156 2573
Fragments arranged by size:
2573 1335 1156 442
BmgBI CAC'GTC
Cuts at: 1318 2497 2689 1318
Size: 1179 192 4135
Fragments arranged by size:
4135 1179 192
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2337 3237 4195
Size: 416 53 83 399 24 920 900 958
Cuts at: 4195 442
Size: 1753
Fragments arranged by size:
1753 958 920 900 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2591 4166 1239
Size: 841 511 1575 2579
Fragments arranged by size:
2579 1575 841 511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2567 3631 4496 4794 2471
Size: 96 1064 865 298 3183
Fragments arranged by size:
3183 1064 865 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2286 2343 4169 882
Size: 617 787 57 1826 2219
Fragments arranged by size:
2219 1826 787 617 57
BsaAI yAC'GTr
Cuts at: 493 1204 2046 2098 2451 2644 3297 493
Size: 711 842 52 353 193 653 2702
Fragments arranged by size:
2702 842 711 653 353 193 52
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2430 3313 3695 4330
Size: 53 83 186 936 897 883 382 635
Cuts at: 4330 275
Size: 1451
Fragments arranged by size:
1451 936 897 883 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5281
Fragments arranged by size:
5281 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2666 1186
Size: 907 573 4026
Fragments arranged by size:
4026 907 573
BseYI C'CCAG_C
Cuts at: 2244 4709 5205 2244
Size: 2465 496 2545
Fragments arranged by size:
2545 2465 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2431 2551 2621 2647 2678 1163
Size: 573 695 120 70 26 31 3991
Fragments arranged by size:
3991 695 573 120 70 31 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2956 3717 4745 1540
Size: 275 186 955 761 1028 2301
Fragments arranged by size:
2301 1028 955 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2709 3567 3652 4723 2070
Size: 639 858 85 1071 2853
Fragments arranged by size:
2853 1071 858 639 85
BsmI GAATG_Cn'
Cuts at: 3017 3110 3017
Size: 93 5413
BsmBI CGTCTCn'nnnn_
Cuts at: 2044 2044
Size: 5506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2232 2709 2825 3567 3652 4723
Size: 399 280 162 477 116 742 85 1071
Cuts at: 4723 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 477 399 280 162 116
85
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 3395 5125 3395
Size: 1730 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2369 2861 844
Size: 1525 492 3489
Fragments arranged by size:
3489 1525 492
BsrBI CCG'CTC
Cuts at: 1394 1823 2626 3393 1394
Size: 429 803 767 3507
Fragments arranged by size:
3507 803 767 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4001 4175 65
Size: 1183 2753 174 1396
Fragments arranged by size:
2753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4150 4337 1700
Size: 110 2340 187 2869
Fragments arranged by size:
2869 2340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 3566 4578 3566
Size: 1012 4494
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2454 794
Size: 1660 3846
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 2323 1184
Size: 1139 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 2749
Size: 5506
BstYI r'GATC_y
Cuts at: 1972 2091 2589 2836 3203 3588 3605 5057 5143
Size: 119 498 247 367 385 17 1452 86
Cuts at: 5143 5155 5501 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 498 385 367 346 247 119
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2296 2593 2754 513
Size: 552 1231 297 161 3265
Fragments arranged by size:
3265 1231 552 297 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4767
Fragments arranged by size:
4767 683 56
BtsI GCAGTG_nn'
Cuts at: 3022 3816 3836 3022
Size: 794 20 4692
Fragments arranged by size:
4692 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2810 2810
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2751 2953
Size: 54 1425 50 275 18 327 594 202
Cuts at: 2953 3842 5471 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 594 327 275 202 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2953 1537
Size: 275 1141 4090
Fragments arranged by size:
4090 1141 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3436 1353
Size: 2083 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2068
Size: 5506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2277 2591 2720 3569 4166 4953
Size: 393 448 197 314 129 849 597 787
Cuts at: 4953 5221 1239
Size: 268 1524
Fragments arranged by size:
1524 849 787 597 448 393 314 268
197 129
EcoNI CCTnn'n_nnAGG
Cuts at: 2786 2786
Size: 5506
EcoO109I rG'GnC_Cy
Cuts at: 2229 2229
Size: 5506
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 2309 2732 4012 2309
Size: 423 1280 3803
Fragments arranged by size:
3803 1280 423
HaeII r_GCGC'y
Cuts at: 1678 2563 4653 1678
Size: 885 2090 2531
Fragments arranged by size:
2531 2090 885
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373
Size: 32 135 32 2575 32 42 32 2626
Fragments arranged by size:
2626 2575 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3096 669
Size: 988 281 912 246 3079
Fragments arranged by size:
3079 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 2613 5382 2613
Size: 2769 2737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4620 4804 1305
Size: 3315 184 2007
Fragments arranged by size:
3315 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3684
Fragments arranged by size:
3684 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2513 3464 3823 3982 518
Size: 648 1149 198 951 359 159 2042
Fragments arranged by size:
2042 1149 951 648 359 198 159
MspA1I CmG'CkG
Cuts at: 1606 2179 2248 2725 2922 3599 4747 4992 1606
Size: 573 69 477 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 573 477 245 197 69
NcoI C'CATG_G
Cuts at: 513 1065 2296 2593 513
Size: 552 1231 297 3426
Fragments arranged by size:
3426 1231 552 297
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2401 2864 4409 1132
Size: 97 1172 463 1545 2229
Fragments arranged by size:
2229 1545 1172 463 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5506
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5506
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PmlI CAC'GTG
Cuts at: 2046 2046
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5506
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PspOMI G'GGCC_C
Cuts at: 2228 2228
Size: 5506
PstI C_TGCA'G
Cuts at: 830 2790 2858 830
Size: 1960 68 3478
Fragments arranged by size:
3478 1960 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
PvuII CAG'CTG
Cuts at: 2248 2248
Size: 5506
SacI G_AGCT'C
Cuts at: 2070 2070
Size: 5506
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SbfI CC_TGCA'GG
Cuts at: 2858 2858
Size: 5506
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SexAI A'CCwGG_T
Cuts at: 2802 2802
Size: 5506
SfcI C'TryA_G
Cuts at: 826 1045 2212 2762 2786 2854 3989 4670 4861
Size: 219 1167 550 24 68 1135 681 191
Cuts at: 4861 826
Size: 1471
Fragments arranged by size:
1471 1167 1135 681 550 219 191 68
24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2227 2833 1872
Size: 355 606 4545
Fragments arranged by size:
4545 606 355
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 2582 3610 4511 4773 820
Size: 197 926 543 96 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 901 543 262 197 96
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2401 2864 1132
Size: 1269 463 3774
Fragments arranged by size:
3774 1269 463
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 2381 2549 1517
Size: 864 168 4474
Fragments arranged by size:
4474 864 168
StyI C'CwwG_G
Cuts at: 513 1065 2296 2329 2593 2945 513
Size: 552 1231 33 264 352 3074
Fragments arranged by size:
3074 1231 552 352 264 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3533 3550 3703 3888 4333 325
Size: 1255 1953 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3752 5464 96
Size: 274 80 33 51 1443 1775 1712 138
Fragments arranged by size:
1775 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5466 5498 5466
Size: 32 5474
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5506
XmaI C'CCGG_G
Cuts at: 1870 2225 2831 1870
Size: 355 606 4545
Fragments arranged by size:
4545 606 355
XmnI GAAnn'nnTTC
Cuts at: 2381 2465 3635 2381
Size: 84 1170 4252
Fragments arranged by size:
4252 1170 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2431 3314 276
Size: 53 83 186 936 897 883 2468
Fragments arranged by size:
2468 936 897 883 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI