Restriction map for
pFN21AB7546
(Circular) (Six-base) MAPSORT of: pFN21AB7546.seq Check: 6726 from: 1 to: 5497
pFN21AB7546 5497 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2130 2130
Size: 5497
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 3560 4944 5212 1632
Size: 705 1223 1384 268 1917
Fragments arranged by size:
1917 1384 1223 705 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 3240 4812 3240
Size: 1572 3925
ApaI G_GGCC'C
Cuts at: 2680 2680
Size: 5497
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2822 1870
Size: 73 879 4545
Fragments arranged by size:
4545 879 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 2504 2818 4273 618
Size: 325 217 627 413 304 314 1455 1842
Fragments arranged by size:
1842 1455 627 413 325 314 304 217
BanII G_rGCy'C
Cuts at: 1391 2680 2816 1391
Size: 1289 136 4072
Fragments arranged by size:
4072 1289 136
BbeI G_GCGC'C
Cuts at: 2204 2204
Size: 5497
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BbvCI CC'TCA_GC
Cuts at: 2495 2495
Size: 5497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2363 2397 3688 3722 1903
Size: 34 50 34 342 34 1291 34 3678
Fragments arranged by size:
3678 1291 342 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4108 136
Size: 107 122 71 631 605 2436 1525
Fragments arranged by size:
2436 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2412 2509 2680 3558 4714 1790
Size: 622 97 171 878 1156 2573
Fragments arranged by size:
2573 1156 878 622 171 97
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4186 442
Size: 416 53 83 399 24 1811 958 1753
Fragments arranged by size:
1811 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2432 2669 4157 1239
Size: 986 207 237 1488 2579
Fragments arranged by size:
2579 1488 986 237 207
Bpu10I CC'TnA_GC
Cuts at: 2495 2782 2495
Size: 287 5210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 3622 4487 4785 2495
Size: 1127 865 298 3207
Fragments arranged by size:
3207 1127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4160 882
Size: 617 2661 2219
Fragments arranged by size:
2661 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 2519 5491 2519
Size: 2972 2525
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2201 3304 3686 4321
Size: 53 83 186 936 668 1103 382 635
Cuts at: 4321 275
Size: 1451
Fragments arranged by size:
1451 1103 936 668 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2143 2601 3930 4328 4602 4749 5160 2143
Size: 458 1329 398 274 147 411 2480
Fragments arranged by size:
2480 1329 458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5272
Fragments arranged by size:
5272 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2335 1186
Size: 1149 4348
BseYI C'CCAG_C
Cuts at: 2211 2304 2312 2424 4700 5196 2211
Size: 93 8 112 2276 496 2512
Fragments arranged by size:
2512 2276 496 112 93 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4924
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2731 3558 3643 4714 2731
Size: 827 85 1071 3514
Fragments arranged by size:
3514 1071 827 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2412 2509 2680 2731 2816 3558 3643
Size: 399 622 97 171 51 85 742 85
Cuts at: 3643 4714 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 622 399 171 97 85
85 51
BspEI T'CCGG_A
Cuts at: 2143 2601 5160 2143
Size: 458 2559 2480
Fragments arranged by size:
2559 2480 458
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2564 2679 2852 844
Size: 1286 434 115 173 3489
Fragments arranged by size:
3489 1286 434 173 115
BsrBI CCG'CTC
Cuts at: 1394 1823 2664 3384 1394
Size: 429 841 720 3507
Fragments arranged by size:
3507 841 720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2326 4141 4328 1700
Size: 110 516 1815 187 2869
Fragments arranged by size:
2869 1815 516 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2478 794
Size: 1684 3813
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstXI CCAn_nnnn'nTGG
Cuts at: 2223 2419 2223
Size: 196 5301
BstYI r'GATC_y
Cuts at: 1972 2148 2443 2827 3194 3579 3596 5048 5134
Size: 176 295 384 367 385 17 1452 86
Cuts at: 5134 5146 5492 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 384 367 346 295 176
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4945
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 2638 3013 3807 3827 2638
Size: 375 794 20 4308
Fragments arranged by size:
4308 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2275 4504 809
Size: 507 959 2229 1802
Fragments arranged by size:
2229 1802 959 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462
Size: 54 1425 50 275 18 1114 889 1629
Cuts at: 5462 8
Size: 43
Fragments arranged by size:
1629 1425 1114 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2365 3427 1353
Size: 1012 1062 3423
Fragments arranged by size:
3423 1062 1012
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2337 2432 2669 3560 4157 4944
Size: 393 593 112 95 237 891 597 787
Cuts at: 4944 5212 1239
Size: 268 1524
Fragments arranged by size:
1524 891 787 597 593 393 268 237
112 95
EcoNI CCTnn'n_nnAGG
Cuts at: 2572 2572
Size: 5497
EcoO109I rG'GnC_Cy
Cuts at: 2577 2577
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2204 4644 1678
Size: 526 2440 2531
Fragments arranged by size:
2531 2440 526
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373
Size: 32 135 32 2566 32 42 32 2626
Fragments arranged by size:
2626 2566 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KasI G'GCGC_C
Cuts at: 2200 2200
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 2217 3096 2217
Size: 879 4618
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2691 4611 4795 1305
Size: 756 630 1920 184 2007
Fragments arranged by size:
2007 1920 756 630 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2697 3455 3814 3973 518
Size: 648 1531 758 359 159 2042
Fragments arranged by size:
2042 1531 758 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2419 2913 3590 4738 4983 1606
Size: 813 494 677 1148 245 2120
Fragments arranged by size:
2120 1148 813 677 494 245
NarI GG'CG_CC
Cuts at: 2201 2201
Size: 5497
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4945
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2253 1348
Size: 905 4592
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2125 1108
Size: 1017 4480
PfoI T'CCnGG_A
Cuts at: 2241 2241
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PspOMI G'GGCC_C
Cuts at: 2676 2676
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2073 2645 2849 830
Size: 1243 572 204 3478
Fragments arranged by size:
3478 1243 572 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
PvuII CAG'CTG
Cuts at: 2419 2419
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2059 2069 2641 2845 3980 4661 4852
Size: 219 1014 10 572 204 1135 681 191
Cuts at: 4852 826
Size: 1471
Fragments arranged by size:
1471 1135 1014 681 572 219 204 191
10
SfoI GGC'GCC
Cuts at: 2202 2202
Size: 5497
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 3601 4502 4764 820
Size: 197 926 567 1091 901 262 1553
Fragments arranged by size:
1553 1091 926 901 567 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 2378 2462 2567 1517
Size: 861 84 105 4447
Fragments arranged by size:
4447 861 105 84
StyI C'CwwG_G
Cuts at: 513 1065 2364 2439 2936 513
Size: 552 1299 75 497 3074
Fragments arranged by size:
3074 1299 552 497 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2492 3524 3541 3694 3879 4324 325
Size: 1255 912 1032 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1032 912 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2756 2788 5457 5489 2756
Size: 32 2669 32 2764
Fragments arranged by size:
2764 2669 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2192 1316
Size: 876 4621
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2181 2181
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 2567 2624 3626 2567
Size: 57 1002 4438
Fragments arranged by size:
4438 1002 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI