Restriction map for
pFN21AB7544
(Circular) (Six-base) MAPSORT of: pFN21AB7544.seq Check: 1644 from: 1 to: 5491
pFN21AB7544 5491 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3301 278
Size: 53 83 186 936 1765 2468
Fragments arranged by size:
2468 1765 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2834 1937
Size: 897 4594
Acc65I G'GTAC_C
Cuts at: 2812 2812
Size: 5491
AclI AA'CG_TT
Cuts at: 1563 3618 3991 1563
Size: 2055 373 3063
Fragments arranged by size:
3063 2055 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2029 3554 4938 5206 1632
Size: 397 1525 1384 268 1917
Fragments arranged by size:
1917 1525 1384 397 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5294
AflIII A'CryG_T
Cuts at: 1203 4390 5367 1203
Size: 3187 977 1327
Fragments arranged by size:
3187 1327 977
AgeI A'CCGG_T
Cuts at: 4322 4322
Size: 5491
AhdI GACnn_n'nnGTC
Cuts at: 2443 4220 2443
Size: 1777 3714
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2525 1184
Size: 1341 4150
AlwNI CAG_nnn'CTG
Cuts at: 2347 2527 2683 3234 4806 2347
Size: 180 156 551 1572 3032
Fragments arranged by size:
3032 1572 551 180 156
ApaLI G'TGCA_C
Cuts at: 3548 4704 3548
Size: 1156 4335
ApoI r'AATT_y
Cuts at: 1493 2800 3026 3178 5287 1493
Size: 1307 226 152 2109 1697
Fragments arranged by size:
2109 1697 1307 226 152
AscI GG'CGCG_CC
Cuts at: 2630 2630
Size: 5491
AseI AT'TA_AT
Cuts at: 160 1032 4045 160
Size: 872 3013 1606
Fragments arranged by size:
3013 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
AvaI C'yCGr_G
Cuts at: 1870 1943 2296 2471 2816 1870
Size: 73 353 175 345 4545
Fragments arranged by size:
4545 353 345 175 73
BamHI G'GATC_C
Cuts at: 2483 2821 3188 2483
Size: 338 367 4786
Fragments arranged by size:
4786 367 338
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2585 2812 4267 618
Size: 325 217 627 798 227 1455 1842
Fragments arranged by size:
1842 1455 798 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2125 2810 1391
Size: 734 685 4072
Fragments arranged by size:
4072 734 685
BbeI G_GCGC'C
Cuts at: 2589 2589
Size: 5491
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2062 2406 2587 928
Size: 971 163 344 181 3832
Fragments arranged by size:
3832 971 344 181 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3682 3716 1903
Size: 34 50 34 1661 34 3678
Fragments arranged by size:
3678 1661 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3383 4593 3383
Size: 1210 4281
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5491
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 2462 2640
Size: 107 122 71 631 605 346 444 178
Cuts at: 2640 4102 136
Size: 1462 1525
Fragments arranged by size:
1525 1462 631 605 444 346 178 122
107 71
BglII A'GATC_T
Cuts at: 5486 5486
Size: 5491
BlpI GC'TnA_GC
Cuts at: 2908 2908
Size: 5491
Bme1580I G_kGCm'C
Cuts at: 1790 3552 4708 1790
Size: 1762 1156 2573
Fragments arranged by size:
2573 1762 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5491
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3222 4180 442
Size: 416 53 83 399 24 1805 958 1753
Fragments arranged by size:
1805 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2340 1056
Size: 1284 4207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2309 2447 4151 1239
Size: 797 273 138 1704 2579
Fragments arranged by size:
2579 1704 797 273 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3616 4481 4779 3616
Size: 865 298 4328
Fragments arranged by size:
4328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2437 4154 882
Size: 617 938 1717 2219
Fragments arranged by size:
2219 1717 938 617
BsaAI yAC'GTr
Cuts at: 493 1204 3282 493
Size: 711 2078 2702
Fragments arranged by size:
2702 2078 711
BsaBI GATnn'nnATC
Cuts at: 5485 5485
Size: 5491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2586 2592 3298
Size: 53 83 186 936 534 519 6 706
Cuts at: 3298 3680 4315 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 706 635 534 519 382 186
83 53 6
BsaWI w'CCGG_w
Cuts at: 3924 4322 4596 4743 5154 3924
Size: 398 274 147 411 4261
Fragments arranged by size:
4261 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5266
Fragments arranged by size:
5266 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 2596 1186
Size: 1222 188 4081
Fragments arranged by size:
4081 1222 188
BseYI C'CCAG_C
Cuts at: 2164 2226 2282 2375 2530 2637 4694 5190 2164
Size: 62 56 93 155 107 2057 496 2465
Fragments arranged by size:
2465 2057 496 155 107 93 62 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2007 1163
Size: 573 271 4647
Fragments arranged by size:
4647 573 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2941 3702 4730 1540
Size: 275 186 940 761 1028 2301
Fragments arranged by size:
2301 1028 940 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3552 3637 4708 3552
Size: 85 1071 4335
Fragments arranged by size:
4335 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 3002 3095 2095
Size: 907 93 4491
Fragments arranged by size:
4491 907 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2125 2810 3552 3637 4708 1391
Size: 399 335 685 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 685 399 335 85
BspEI T'CCGG_A
Cuts at: 5154 5154
Size: 5491
BspHI T'CATG_A
Cuts at: 3380 5110 3380
Size: 1730 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2846 844
Size: 2002 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3378 1394
Size: 429 1555 3507
Fragments arranged by size:
3507 1555 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3986 4160 65
Size: 1183 2738 174 1396
Fragments arranged by size:
2738 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2588 2714 4135 4322 1700
Size: 110 778 126 1421 187 2869
Fragments arranged by size:
2869 1421 778 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5491
BssHII G'CGCG_C
Cuts at: 1925 2630 2687 2689 2763 1925
Size: 705 57 2 74 4653
Fragments arranged by size:
4653 705 74 57 2
BssSI C'ACGA_G
Cuts at: 2459 3551 4563 2459
Size: 1092 1012 3387
Fragments arranged by size:
3387 1092 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5491
BstBI TT'CG_AA
Cuts at: 4295 4295
Size: 5491
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5491
BstYI r'GATC_y
Cuts at: 1972 2483 2721 2821 3188 3573 3590 5042 5128
Size: 511 238 100 367 385 17 1452 86
Cuts at: 5128 5140 5486 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 511 385 367 346 238 100
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2322 513
Size: 552 1257 3682
Fragments arranged by size:
3682 1257 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4752
Fragments arranged by size:
4752 683 56
BtsI GCAGTG_nn'
Cuts at: 3007 3801 3821 3007
Size: 794 20 4677
Fragments arranged by size:
4677 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5456
DraI TTT'AAA
Cuts at: 2795 2795
Size: 5491
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4498 809
Size: 507 3182 1802
Fragments arranged by size:
3182 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2938 3827 5456
Size: 54 1425 50 275 18 1108 889 1629
Cuts at: 5456 8
Size: 43
Fragments arranged by size:
1629 1425 1108 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2938 1537
Size: 275 1126 4090
Fragments arranged by size:
4090 1126 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3421 1353
Size: 2068 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2771 4078 4452 4598 387
Size: 1064 138 1182 1307 374 146 1280
Fragments arranged by size:
1307 1280 1182 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2036 2309 2447 3554 4151 4938
Size: 393 397 7 273 138 1107 597 787
Cuts at: 4938 5206 1239
Size: 268 1524
Fragments arranged by size:
1524 1107 787 597 397 393 273 268
138 7
EcoO109I rG'GnC_Cy
Cuts at: 2293 2300 2455 2293
Size: 7 155 5329
Fragments arranged by size:
5329 155 7
EcoRI G'AATT_C
Cuts at: 2800 3178 2800
Size: 378 5113
FspI TGC'GCA
Cuts at: 3997 3997
Size: 5491
HaeII r_GCGC'y
Cuts at: 1678 2589 4638 1678
Size: 911 2049 2531
Fragments arranged by size:
2531 2049 911
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 4132 4164 4206
Size: 32 135 32 857 32 1671 32 42
Cuts at: 4206 4238 1373
Size: 32 2626
Fragments arranged by size:
2626 1671 857 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2835 3081 669
Size: 988 281 897 246 3079
Fragments arranged by size:
3079 988 897 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5491
HpaI GTT'AAC
Cuts at: 3081 3081
Size: 5491
KasI G'GCGC_C
Cuts at: 2585 2585
Size: 5491
KpnI G_GTAC'C
Cuts at: 2816 2816
Size: 5491
MfeI C'AATT_G
Cuts at: 3090 3090
Size: 5491
MluI A'CGCG_T
Cuts at: 5367 5367
Size: 5491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 2427 4605 4789 1305
Size: 1086 36 2178 184 2007
Fragments arranged by size:
2178 2007 1086 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3669
Fragments arranged by size:
3669 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3449 3808 3967 518
Size: 648 2283 359 159 2042
Fragments arranged by size:
2283 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2088 2637 2907 3584 4732 4977 1606
Size: 443 39 549 270 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 549 443 270 245 39
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 5491
NarI GG'CG_CC
Cuts at: 2586 2586
Size: 5491
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4939
NdeI CA'TA_TG
Cuts at: 387 2362 387
Size: 1975 3516
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 5491
NheI G'CTAG_C
Cuts at: 1052 2336 1052
Size: 1284 4207
NotI GC'GGCC_GC
Cuts at: 2938 2938
Size: 5491
NspI r_CATG'y
Cuts at: 1132 1229 2557 2849 4394 1132
Size: 97 1328 292 1545 2229
Fragments arranged by size:
2229 1545 1328 292 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5491
PciI A'CATG_T
Cuts at: 4390 4390
Size: 5491
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 2683 1108
Size: 1239 336 3916
Fragments arranged by size:
3916 1239 336
PmeI GTTT'AAAC
Cuts at: 2795 2795
Size: 5491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 5073 5105 3560
Size: 32 1481 32 3946
Fragments arranged by size:
3946 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5491
PsiI TTA'TAA
Cuts at: 3061 3061
Size: 5491
PstI C_TGCA'G
Cuts at: 830 2263 2779 2843 830
Size: 1433 516 64 3478
Fragments arranged by size:
3478 1433 516 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5491
PvuII CAG'CTG
Cuts at: 2049 2088 2049
Size: 39 5452
SalI G'TCGA_C
Cuts at: 1936 2833 1936
Size: 897 4594
SbfI CC_TGCA'GG
Cuts at: 2263 2843 2263
Size: 580 4911
ScaI AGT'ACT
Cuts at: 3739 5451 3739
Size: 1712 3779
SfcI C'TryA_G
Cuts at: 826 1045 2251 2259 2775 2839 3974 4655 4846
Size: 219 1206 8 516 64 1135 681 191
Cuts at: 4846 826
Size: 1471
Fragments arranged by size:
1471 1206 1135 681 516 219 191 64
8
SfoI GGC'GCC
Cuts at: 2587 2587
Size: 5491
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
SgrAI Cr'CCGG_yG
Cuts at: 1700 2588 1700
Size: 888 4603
SmaI CCC'GGG
Cuts at: 1872 2298 2818 1872
Size: 426 520 4545
Fragments arranged by size:
4545 520 426
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3595 4496 4758 820
Size: 197 926 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3282 493
Size: 2789 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5491
SphI G_CATG'C
Cuts at: 1132 2849 1132
Size: 1717 3774
SspI AAT'ATT
Cuts at: 5 52 3415 5
Size: 47 3363 2081
Fragments arranged by size:
3363 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5491
StyI C'CwwG_G
Cuts at: 513 1065 2930 513
Size: 552 1865 3074
Fragments arranged by size:
3074 1865 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2563 3518 3535 3688 3873 4318 325
Size: 1255 983 955 17 153 185 445 1498
Fragments arranged by size:
1498 1255 983 955 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3737 5449 96
Size: 274 80 33 51 1443 1760 1712 138
Fragments arranged by size:
1760 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5451 5483 5451
Size: 32 5459
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5491
XbaI T'CTAG_A
Cuts at: 2827 2827
Size: 5491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5491
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5491
XmaI C'CCGG_G
Cuts at: 1870 2296 2816 1870
Size: 426 520 4545
Fragments arranged by size:
4545 520 426
XmnI GAAnn'nnTTC
Cuts at: 3620 3620
Size: 5491
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3299 276
Size: 53 83 186 936 1765 2468
Fragments arranged by size:
2468 1765 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI