Restriction map for
pFN21AB7540
(Circular) (Six-base) MAPSORT of: pFN21AB7540.seq Check: 78 from: 1 to: 5479
pFN21AB7540 5479 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3542 4926 5194 1632
Size: 1910 1384 268 1917
Fragments arranged by size:
1917 1910 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 2350 2551 2587 2677 3222 4794 2350
Size: 201 36 90 545 1572 3035
Fragments arranged by size:
3035 1572 545 201 90 36
ApaI G_GGCC'C
Cuts at: 2278 2580 2739 2278
Size: 302 159 5018
Fragments arranged by size:
5018 302 159
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 2146 2166 2482 2739 2804 1870
Size: 73 185 18 20 316 257 65 4545
Fragments arranged by size:
4545 316 257 185 73 65 20 18
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2563 2800 4255 618
Size: 325 217 627 375 401 237 1455 1842
Fragments arranged by size:
1842 1455 627 401 375 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2175 2278 2357 2580 2739 2798 1391
Size: 784 103 79 223 159 59 4072
Fragments arranged by size:
4072 784 223 159 103 79 59
BbeI G_GCGC'C
Cuts at: 2166 2166
Size: 5479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 2514 928
Size: 971 424 191 3893
Fragments arranged by size:
3893 971 424 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3670 3704 1903
Size: 34 50 34 1649 34 3678
Fragments arranged by size:
3678 1649 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BfrBI ATG'CAT
Cuts at: 2774 2774
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 4090 136
Size: 107 122 71 631 605 470 1948 1525
Fragments arranged by size:
1948 1525 631 605 470 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2278 2558 2566 2580 2739 3540 4696 1790
Size: 488 280 8 14 159 801 1156 2573
Fragments arranged by size:
2573 1156 801 488 280 159 14 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2381 4139 1239
Size: 793 349 1758 2579
Fragments arranged by size:
2579 1758 793 349
Bpu10I CC'TnA_GC
Cuts at: 2089 2351 2089
Size: 262 5217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2493 2621 4142 882
Size: 617 994 128 1521 2219
Fragments arranged by size:
2219 1521 994 617 128
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2328 2681 3286
Size: 53 83 186 936 630 165 353 605
Cuts at: 3286 3668 4303 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 630 605 382 353 186
165 83 53
BsaWI w'CCGG_w
Cuts at: 3912 4310 4584 4731 5142 3912
Size: 398 274 147 411 4249
Fragments arranged by size:
4249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 1178
Size: 30 165 30 654 30 4570
Fragments arranged by size:
4570 654 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2005 2008 1186
Size: 819 3 4657
Fragments arranged by size:
4657 819 3
BseYI C'CCAG_C
Cuts at: 2463 2503 2713 4682 5178 2463
Size: 40 210 1969 496 2764
Fragments arranged by size:
2764 1969 496 210 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 1163
Size: 573 717 4189
Fragments arranged by size:
4189 717 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3540 3625 4696 3540
Size: 85 1071 4323
Fragments arranged by size:
4323 1071 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2278 2357 2558 2566 2580 2739
Size: 399 385 103 79 201 8 14 159
Cuts at: 2739 2798 3540 3625 4696 1391
Size: 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 385 201 159 103
85 79 59 14 8
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 2834 844
Size: 1455 535 3489
Fragments arranged by size:
3489 1455 535
BsrBI CCG'CTC
Cuts at: 1394 1823 2365 3366 1394
Size: 429 542 1001 3507
Fragments arranged by size:
3507 1001 542 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2703 4123 4310 1700
Size: 110 893 1420 187 2869
Fragments arranged by size:
2869 1420 893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 2028 3539 4551 2028
Size: 1511 1012 2956
Fragments arranged by size:
2956 1511 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2178 2178
Size: 5479
BtgI C'CryG_G
Cuts at: 513 1065 2061 2238 2301 2730 513
Size: 552 996 177 63 429 3262
Fragments arranged by size:
3262 996 552 429 177 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3789 3809 2995
Size: 794 20 4665
Fragments arranged by size:
4665 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DraIII CAC_nnn'GTG
Cuts at: 2030 2030
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2376 2926
Size: 54 1425 50 275 18 411 135 550
Cuts at: 2926 3815 5444 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 550 411 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2363 3409 1353
Size: 1010 1046 3423
Fragments arranged by size:
3423 1046 1010
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2381 3542 4139 4926 5194 1239
Size: 393 400 349 1161 597 787 268 1524
Fragments arranged by size:
1524 1161 787 597 400 393 349 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2697 2697
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2208 2275 2458 2577 2638 2735 2736 2743 2208
Size: 67 183 119 61 97 1 7 4944
Fragments arranged by size:
4944 183 119 97 67 61 7 1
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FseI GG_CCGG'CC
Cuts at: 2707 2707
Size: 5479
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2166 4626 1678
Size: 488 2460 2531
Fragments arranged by size:
2531 2460 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 4120 4152 4194
Size: 32 135 32 485 32 2031 32 42
Cuts at: 4194 4226 1373
Size: 32 2626
Fragments arranged by size:
2626 2031 485 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KasI G'GCGC_C
Cuts at: 2162 2162
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3111
Fragments arranged by size:
3111 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3796 3955 518
Size: 648 2271 359 159 2042
Fragments arranged by size:
2271 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2467 2548 2895 3572 4720 4965 1606
Size: 479 382 81 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 479 382 347 245 81
NaeI GCC'GGC
Cuts at: 2705 2705
Size: 5479
NarI GG'CG_CC
Cuts at: 2163 2163
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 2238 2301 513
Size: 552 1173 63 3691
Fragments arranged by size:
3691 1173 552 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NgoMIV G'CCGG_C
Cuts at: 2703 2703
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NsiI A_TGCA'T
Cuts at: 2776 2776
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2030 1108
Size: 922 4557
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2208 2458 2208
Size: 250 5229
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2274 2576 2735 2274
Size: 302 159 5018
Fragments arranged by size:
5018 302 159
PstI C_TGCA'G
Cuts at: 830 2547 2831 830
Size: 1717 284 3478
Fragments arranged by size:
3478 1717 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2085 2467 2548 2085
Size: 382 81 5016
Fragments arranged by size:
5016 382 81
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SexAI A'CCwGG_T
Cuts at: 2414 2414
Size: 5479
SfcI C'TryA_G
Cuts at: 826 1045 2543 2827 3962 4643 4834 826
Size: 219 1498 284 1135 681 191 1471
Fragments arranged by size:
1498 1471 1135 681 284 219 191
SfoI GGC'GCC
Cuts at: 2164 2164
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2130 2148 2168 2741 2806 1872
Size: 258 18 20 573 65 4545
Fragments arranged by size:
4545 573 258 65 20 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SrfI GCCC'GGGC
Cuts at: 2148 2148
Size: 5479
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 2113 1517
Size: 596 4883
StyI C'CwwG_G
Cuts at: 513 1065 2238 2301 2319 2558 2918 513
Size: 552 1173 63 18 239 360 3074
Fragments arranged by size:
3074 1173 552 360 239 63 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 3523 3676 3861 4306 325
Size: 1255 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2128 2146 2166 2739 2804 1870
Size: 258 18 20 573 65 4545
Fragments arranged by size:
4545 573 258 65 20 18
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FspAI
NruI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SwaI XcmI