Restriction map for
pFN21AB7536
(Circular) (Six-base) MAPSORT of: pFN21AB7536.seq Check: 2732 from: 1 to: 5476
pFN21AB7536 5476 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2711 3286 278
Size: 53 83 186 936 1175 575 2468
Fragments arranged by size:
2468 1175 936 575 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2395 2797 2395
Size: 402 5074
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2465 1184
Size: 1281 4195
AlwNI CAG_nnn'CTG
Cuts at: 2410 3219 4791 2410
Size: 809 1572 3095
Fragments arranged by size:
3095 1572 809
ApaI G_GGCC'C
Cuts at: 2294 2372 2294
Size: 78 5398
ApaLI G'TGCA_C
Cuts at: 2594 3533 4689 2594
Size: 939 1156 3381
Fragments arranged by size:
3381 1156 939
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2528 2801 1870
Size: 73 585 273 4545
Fragments arranged by size:
4545 585 273 73
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2150 2168 2281 2324
Size: 325 217 627 351 12 18 113 43
Cuts at: 2324 2395 2490 2797 4252 618
Size: 71 95 307 1455 1842
Fragments arranged by size:
1842 1455 627 351 325 307 217 113
95 71 43 18 12
BanII G_rGCy'C
Cuts at: 1391 2294 2372 2795 1391
Size: 903 78 423 4072
Fragments arranged by size:
4072 903 423 78
BbeI G_GCGC'C
Cuts at: 2142 2154 2172 2285 2328 2142
Size: 12 18 113 43 5290
Fragments arranged by size:
5290 113 43 18 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 928
Size: 971 199 4306
Fragments arranged by size:
4306 971 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3667 3701 1903
Size: 34 50 34 1646 34 3678
Fragments arranged by size:
3678 1646 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 2294 2372 2432 2570 2598 3537 4693 1790
Size: 504 78 60 138 28 939 1156 2573
Fragments arranged by size:
2573 1156 939 504 138 78 60 28
BmgBI CAC'GTC
Cuts at: 1318 2563 1318
Size: 1245 4231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4136 1239
Size: 2897 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4139 882
Size: 617 1041 1599 2219
Fragments arranged by size:
2219 1599 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 2272 3267 493
Size: 711 1068 995 2702
Fragments arranged by size:
2702 1068 995 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2151 2169 2282
Size: 53 83 186 936 606 12 18 113
Cuts at: 2282 2325 2351 2708 2723 3283 3665 4300 275
Size: 43 26 357 15 560 382 635 1451
Fragments arranged by size:
1451 936 635 606 560 382 357 186
113 83 53 43 26 18 15 12
BsaWI w'CCGG_w
Cuts at: 2728 3909 4307 4581 4728 5139 2728
Size: 1181 398 274 147 411 3065
Fragments arranged by size:
3065 1181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 2609 2639 1178
Size: 30 165 30 1134 30 42 30 4015
Fragments arranged by size:
4015 1134 165 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2621 2690 1186
Size: 960 475 69 3972
Fragments arranged by size:
3972 960 475 69
BseYI C'CCAG_C
Cuts at: 2320 2328 4679 5175 2320
Size: 8 2351 496 2621
Fragments arranged by size:
2621 2351 496 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 2252 2294 2453 1163
Size: 573 366 150 42 159 4186
Fragments arranged by size:
4186 573 366 159 150 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2051 2217 2926 3687 4715 1540
Size: 275 186 50 166 709 761 1028 2301
Fragments arranged by size:
2301 1028 761 709 275 186 166 50
BsiHKAI G_wGCw'C
Cuts at: 2339 2598 3537 3622 4693 2339
Size: 259 939 85 1071 3122
Fragments arranged by size:
3122 1071 939 259 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 2339 2372 2432 2570 2598 2795
Size: 399 504 45 33 60 138 28 197
Cuts at: 2795 3537 3622 4693 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 197 138 85
60 45 33 28
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2831 844
Size: 1987 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 1394
Size: 429 1540 3507
Fragments arranged by size:
3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 4120 4307 1700
Size: 110 264 2046 187 2869
Fragments arranged by size:
2869 2046 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2407 794
Size: 1613 3863
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471
Size: 834 367 385 17 1452 86 12 346
Cuts at: 5471 1972
Size: 1977
Fragments arranged by size:
1977 1452 834 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2187 2217 2286 513
Size: 552 1122 30 69 3703
Fragments arranged by size:
3703 1122 552 69 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2339 2992 3786 3806 2339
Size: 653 794 20 4009
Fragments arranged by size:
4009 794 653 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2713 4483 809
Size: 507 1397 1770 1802
Fragments arranged by size:
1802 1770 1397 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 2214 2923
Size: 54 1425 50 275 18 218 166 709
Cuts at: 2923 3812 5441 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 709 275 218 166 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2048 2214 2923 1537
Size: 275 236 166 709 4090
Fragments arranged by size:
4090 709 275 236 166
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 2729 2736 3406 1353
Size: 1190 186 7 670 3423
Fragments arranged by size:
3423 1190 670 186 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2494 4063 4437 4583 387
Size: 1064 138 905 1569 374 146 1280
Fragments arranged by size:
1569 1280 1064 905 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3539 4136 4923 5191 1239
Size: 393 1907 597 787 268 1524
Fragments arranged by size:
1907 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2603 2540
Size: 63 5413
EcoO109I rG'GnC_Cy
Cuts at: 2368 2369 2368
Size: 1 5475
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2142 2154 2172 2285 2328 4623 1678
Size: 464 12 18 113 43 2295 2531
Fragments arranged by size:
2531 2295 464 113 43 18 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2782 4117 4149 4191
Size: 32 135 32 1178 32 1335 32 42
Cuts at: 4191 4223 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 1178 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KasI G'GCGC_C
Cuts at: 2138 2150 2168 2281 2324 2138
Size: 12 18 113 43 5290
Fragments arranged by size:
5290 113 43 18 12
KpnI G_GTAC'C
Cuts at: 2399 2801 2399
Size: 402 5074
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 4590 4774 1305
Size: 1356 1929 184 2007
Fragments arranged by size:
2007 1929 1356 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3654
Fragments arranged by size:
3654 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3434 3793 3952 518
Size: 648 1257 1011 359 159 2042
Fragments arranged by size:
2042 1257 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2145 2219 2226 2266 2320 2467 2581 2892
Size: 539 74 7 40 54 147 114 311
Cuts at: 2892 3569 4717 4962 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 539 311 245 147 114
74 54 40 7
NarI GG'CG_CC
Cuts at: 2139 2151 2169 2282 2325 2139
Size: 12 18 113 43 5290
Fragments arranged by size:
5290 113 43 18 12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4924
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2020 2834 4379 1132
Size: 97 791 814 1545 2229
Fragments arranged by size:
2229 1545 814 791 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5476
PfoI T'CCnGG_A
Cuts at: 2541 2541
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PmlI CAC'GTG
Cuts at: 2272 2272
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PspOMI G'GGCC_C
Cuts at: 2290 2368 2290
Size: 78 5398
PstI C_TGCA'G
Cuts at: 830 2454 2828 830
Size: 1624 374 3478
Fragments arranged by size:
3478 1624 374
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2266 2467 2581 2266
Size: 201 114 5161
Fragments arranged by size:
5161 201 114
SacII CC_GC'GG
Cuts at: 2220 2220
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2450 2824 3959 4640 4831 826
Size: 219 1405 374 1135 681 191 1471
Fragments arranged by size:
1471 1405 1135 681 374 219 191
SfoI GGC'GCC
Cuts at: 2140 2152 2170 2283 2326 2140
Size: 12 18 113 43 5290
Fragments arranged by size:
5290 113 43 18 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 3580 4481 4743 820
Size: 197 926 585 1052 901 262 1553
Fragments arranged by size:
1553 1052 926 901 585 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2915 513
Size: 552 1850 3074
Fragments arranged by size:
3074 1850 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5436 5468 5436
Size: 32 5444
Tth111I GACn'n_nGTC
Cuts at: 1316 2710 1316
Size: 1394 4082
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 2528 1943
Size: 585 4891
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 2537 3605 2537
Size: 1068 4408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2709 3284 276
Size: 53 83 186 936 1175 575 2468
Fragments arranged by size:
2468 1175 936 575 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI XcmI