Restriction map for
pFN21AB7532
(Circular) (Six-base) MAPSORT of: pFN21AB7532.seq Check: 9357 from: 1 to: 5473
pFN21AB7532 5473 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2146 2146
Size: 5473
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3283 278
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2639 3536 4920 5188 1632
Size: 1007 897 1384 268 1917
Fragments arranged by size:
1917 1384 1007 897 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 2367 4372 5349 1203
Size: 1164 2005 977 1327
Fragments arranged by size:
2005 1327 1164 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 2189 2402 1166
Size: 1023 213 4237
Fragments arranged by size:
4237 1023 213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5473
AlwNI CAG_nnn'CTG
Cuts at: 2138 2173 2299 3216 4788 2138
Size: 35 126 917 1572 2823
Fragments arranged by size:
2823 1572 917 126 35
ApaI G_GGCC'C
Cuts at: 2412 2493 2507 2613 2412
Size: 81 14 106 5272
Fragments arranged by size:
5272 106 81 14
ApaLI G'TGCA_C
Cuts at: 3530 4686 3530
Size: 1156 4317
ApoI r'AATT_y
Cuts at: 1493 2782 3008 3160 5269 1493
Size: 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2798 1870
Size: 73 855 4545
Fragments arranged by size:
4545 855 73
BamHI G'GATC_C
Cuts at: 2803 3170 2803
Size: 367 5106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2518 2794 4249 618
Size: 325 217 627 731 276 1455 1842
Fragments arranged by size:
1842 1455 731 627 325 276 217
BanII G_rGCy'C
Cuts at: 1391 2412 2493 2507 2613 2792 1391
Size: 1021 81 14 106 179 4072
Fragments arranged by size:
4072 1021 179 106 81 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2673 928
Size: 971 774 3728
Fragments arranged by size:
3728 971 774
BbvCI CC'TCA_GC
Cuts at: 2099 2544 2099
Size: 445 5028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2720 2754 3664 3698 1903
Size: 34 50 34 699 34 910 34 3678
Fragments arranged by size:
3678 910 699 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4084 136
Size: 107 122 71 631 605 2412 1525
Fragments arranged by size:
2412 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2890 2890
Size: 5473
Bme1580I G_kGCm'C
Cuts at: 1790 2334 2390 2412 2493 2507 2613 3534 4690
Size: 544 56 22 81 14 106 921 1156
Cuts at: 4690 1790
Size: 2573
Fragments arranged by size:
2573 1156 921 544 106 81 56 22
14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2608 3204 4162
Size: 416 53 83 399 24 1191 596 958
Cuts at: 4162 442
Size: 1753
Fragments arranged by size:
1753 1191 958 596 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 4133 1239
Size: 1280 1614 2579
Fragments arranged by size:
2579 1614 1280
Bpu10I CC'TnA_GC
Cuts at: 2099 2544 2099
Size: 445 5028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3598 4463 4761 3598
Size: 865 298 4310
Fragments arranged by size:
4310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4136 882
Size: 617 2637 2219
Fragments arranged by size:
2637 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3264 493
Size: 711 2060 2702
Fragments arranged by size:
2702 2060 711
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2733 3280 3662
Size: 53 83 186 936 534 666 547 382
Cuts at: 3662 4297 275
Size: 635 1451
Fragments arranged by size:
1451 936 666 635 547 534 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2029 3906 4304 4578 4725 5136 2029
Size: 1877 398 274 147 411 2366
Fragments arranged by size:
2366 1877 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5248
Fragments arranged by size:
5248 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2255 2404 1186
Size: 930 139 149 4255
Fragments arranged by size:
4255 930 149 139
BseYI C'CCAG_C
Cuts at: 2041 2271 2390 2596 4676 5172 2041
Size: 230 119 206 2080 496 2342
Fragments arranged by size:
2342 2080 496 230 206 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 2396 1163
Size: 573 388 272 4240
Fragments arranged by size:
4240 573 388 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 3684 4712 1540
Size: 275 186 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2514 3534 3619 4690 2514
Size: 1020 85 1071 3297
Fragments arranged by size:
3297 1071 1020 85
BsmI GAATG_Cn'
Cuts at: 2984 3077 2984
Size: 93 5380
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2334 2390 2412 2493 2507 2514 2613
Size: 399 544 56 22 81 14 7 99
Cuts at: 2613 2792 3534 3619 4690 1391
Size: 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 399 179 99 85
81 56 22 14 7
BspEI T'CCGG_A
Cuts at: 2029 5136 2029
Size: 3107 2366
BspHI T'CATG_A
Cuts at: 3362 5092 3362
Size: 1730 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2146 2828 844
Size: 1302 682 3489
Fragments arranged by size:
3489 1302 682
BsrBI CCG'CTC
Cuts at: 1394 1823 3360 1394
Size: 429 1537 3507
Fragments arranged by size:
3507 1537 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3968 4142 65
Size: 1183 2720 174 1396
Fragments arranged by size:
2720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2258 4117 4304 1700
Size: 110 448 1859 187 2869
Fragments arranged by size:
2869 1859 448 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 3533 4545 3533
Size: 1012 4461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2138 794
Size: 1344 4129
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468
Size: 831 367 385 17 1452 86 12 346
Cuts at: 5468 1972
Size: 1977
Fragments arranged by size:
1977 1452 831 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2306 2537 513
Size: 552 1241 231 3449
Fragments arranged by size:
3449 1241 552 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4734
Fragments arranged by size:
4734 683 56
BtsI GCAGTG_nn'
Cuts at: 2131 2238 2490 2989 3783 3803 2131
Size: 107 252 499 794 20 3801
Fragments arranged by size:
3801 794 499 252 107 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4480 809
Size: 507 3164 1802
Fragments arranged by size:
3164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 2283 2920
Size: 54 1425 50 275 18 224 229 637
Cuts at: 2920 3809 5438 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 637 275 229 224 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2920 1537
Size: 275 1108 4090
Fragments arranged by size:
4090 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2671 2715 3403 1353
Size: 1318 44 688 3423
Fragments arranged by size:
3423 1318 688 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2427 2461 4060 4434 4580 387
Size: 1064 138 838 34 1599 374 146 1280
Fragments arranged by size:
1599 1280 1064 838 374 146 138 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2639 3536 4133 4920 5188 1239
Size: 393 887 120 897 597 787 268 1524
Fragments arranged by size:
1524 897 887 787 597 393 268 120
EcoO109I rG'GnC_Cy
Cuts at: 2195 2408 2409 2489 2490 2503 2610 2195
Size: 213 1 80 1 13 107 5058
Fragments arranged by size:
5058 213 107 80 13 1 1
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 4620 1678
Size: 2942 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373
Size: 32 135 32 2542 32 42 32 2626
Fragments arranged by size:
2626 2542 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2424 2694 2817 3063 669
Size: 988 281 486 270 123 246 3079
Fragments arranged by size:
3079 988 486 281 270 246 123
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4587 4771 1305
Size: 3282 184 2007
Fragments arranged by size:
3282 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3651
Fragments arranged by size:
3651 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2372 2402 3431 3790 3949 518
Size: 648 1023 183 30 1029 359 159 2042
Fragments arranged by size:
2042 1029 1023 648 359 183 159 30
MspA1I CmG'CkG
Cuts at: 1606 2349 2363 2497 2889 3566 4714 4959 1606
Size: 743 14 134 392 677 1148 245 2120
Fragments arranged by size:
2120 1148 743 677 392 245 134 14
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 5473
NcoI C'CATG_G
Cuts at: 513 1065 2306 513
Size: 552 1241 3680
Fragments arranged by size:
3680 1241 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 5473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2371 2831 4376 1132
Size: 97 1142 460 1545 2229
Fragments arranged by size:
2229 1545 1142 460 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 2367 4372 2367
Size: 2005 3468
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4571
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 5055 5087 3542
Size: 32 1481 32 3928
Fragments arranged by size:
3928 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2195 2195
Size: 5473
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PspOMI G'GGCC_C
Cuts at: 2408 2489 2503 2609 2408
Size: 81 14 106 5272
Fragments arranged by size:
5272 106 81 14
PstI C_TGCA'G
Cuts at: 830 2233 2295 2580 2825 830
Size: 1403 62 285 245 3478
Fragments arranged by size:
3478 1403 285 245 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
PvuII CAG'CTG
Cuts at: 2349 2363 2349
Size: 14 5459
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SapI GCTCTTCn'nnn_
Cuts at: 2671 2671
Size: 5473
SbfI CC_TGCA'GG
Cuts at: 2825 2825
Size: 5473
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SfcI C'TryA_G
Cuts at: 826 1045 2229 2291 2576 2821 3956 4637 4828
Size: 219 1184 62 285 245 1135 681 191
Cuts at: 4828 826
Size: 1471
Fragments arranged by size:
1471 1184 1135 681 285 245 219 191
62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2800 1872
Size: 928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3577 4478 4740 820
Size: 197 926 1634 901 262 1553
Fragments arranged by size:
1634 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5473
StyI C'CwwG_G
Cuts at: 513 1065 2306 2912 513
Size: 552 1241 606 3074
Fragments arranged by size:
3074 1241 606 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3500 3517 3670 3855 4300 325
Size: 1255 1920 17 153 185 445 1498
Fragments arranged by size:
1920 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3719 5431 96
Size: 274 80 33 51 1443 1742 1712 138
Fragments arranged by size:
1742 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2179 2211 5433 5465 2179
Size: 32 3222 32 2187
Fragments arranged by size:
3222 2187 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5473
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2398 2482 2123
Size: 275 84 5114
Fragments arranged by size:
5114 275 84
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2798 1870
Size: 928 4545
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3281 276
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI