Restriction map for
pFN21AB7527
(Circular) (Six-base) MAPSORT of: pFN21AB7527.seq Check: 5907 from: 1 to: 5170
pFN21AB7527 5170 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2980 278
Size: 53 83 186 936 1444 2468
Fragments arranged by size:
2468 1444 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2513 1937
Size: 576 4594
Acc65I G'GTAC_C
Cuts at: 2491 2491
Size: 5170
AclI AA'CG_TT
Cuts at: 1563 3297 3670 1563
Size: 1734 373 3063
Fragments arranged by size:
3063 1734 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3233 4617 4885 1632
Size: 1601 1384 268 1917
Fragments arranged by size:
1917 1601 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4973
AflIII A'CryG_T
Cuts at: 1203 4069 5046 1203
Size: 2866 977 1327
Fragments arranged by size:
2866 1327 977
AgeI A'CCGG_T
Cuts at: 4001 4001
Size: 5170
AhdI GACnn_n'nnGTC
Cuts at: 3899 3899
Size: 5170
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5170
AlwNI CAG_nnn'CTG
Cuts at: 2913 4485 2913
Size: 1572 3598
ApaLI G'TGCA_C
Cuts at: 3227 4383 3227
Size: 1156 4014
ApoI r'AATT_y
Cuts at: 1493 2275 2284 2479 2705 2857 4966 1493
Size: 782 9 195 226 152 2109 1697
Fragments arranged by size:
2109 1697 782 226 195 152 9
AseI AT'TA_AT
Cuts at: 160 1032 3724 160
Size: 872 2692 1606
Fragments arranged by size:
2692 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5170
AvaI C'yCGr_G
Cuts at: 1870 1943 2495 1870
Size: 73 552 4545
Fragments arranged by size:
4545 552 73
BamHI G'GATC_C
Cuts at: 2500 2867 2500
Size: 367 4803
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2491 3946 618
Size: 325 217 627 704 1455 1842
Fragments arranged by size:
1842 1455 704 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2489 1391
Size: 1098 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2262 2373 928
Size: 971 363 111 3725
Fragments arranged by size:
3725 971 363 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3361 3395 1903
Size: 34 1424 34 3678
Fragments arranged by size:
3678 1424 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3062 4272 3062
Size: 1210 3960
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5170
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3781 136
Size: 107 122 71 631 605 2109 1525
Fragments arranged by size:
2109 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5165 5165
Size: 5170
BlpI GC'TnA_GC
Cuts at: 2587 2587
Size: 5170
Bme1580I G_kGCm'C
Cuts at: 1790 2088 3231 4387 1790
Size: 298 1143 1156 2573
Fragments arranged by size:
2573 1156 1143 298
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2901 3859 442
Size: 416 53 83 399 24 1484 958 1753
Fragments arranged by size:
1753 1484 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3830 1239
Size: 2591 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3295 4160 4458 3295
Size: 865 298 4007
Fragments arranged by size:
4007 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3833 882
Size: 617 2334 2219
Fragments arranged by size:
2334 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2961 493
Size: 711 1757 2702
Fragments arranged by size:
2702 1757 711
BsaBI GATnn'nnATC
Cuts at: 5164 5164
Size: 5170
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2977 3359 3994
Size: 53 83 186 936 476 968 382 635
Cuts at: 3994 275
Size: 1451
Fragments arranged by size:
1451 968 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2109 3603 4001 4275 4422 4833 2109
Size: 1494 398 274 147 411 2446
Fragments arranged by size:
2446 1494 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4945
Fragments arranged by size:
4945 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 1186
Size: 1291 3879
BseYI C'CCAG_C
Cuts at: 4373 4869 4373
Size: 496 4674
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4597
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2620 3381 4409 1540
Size: 275 186 619 761 1028 2301
Fragments arranged by size:
2301 1028 761 619 275 186
BsiHKAI G_wGCw'C
Cuts at: 3231 3316 4387 3231
Size: 85 1071 4014
Fragments arranged by size:
4014 1071 85
BsmI GAATG_Cn'
Cuts at: 2681 2774 2681
Size: 93 5077
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2489 3231 3316 4387 1391
Size: 399 298 401 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 298 85
BspEI T'CCGG_A
Cuts at: 2109 4833 2109
Size: 2724 2446
BspHI T'CATG_A
Cuts at: 2382 3059 4789 2382
Size: 677 1730 2763
Fragments arranged by size:
2763 1730 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2525 844
Size: 1681 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3057 1394
Size: 429 1234 3507
Fragments arranged by size:
3507 1234 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3665 3839 65
Size: 1183 2417 174 1396
Fragments arranged by size:
2417 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3814 4001 1700
Size: 110 2004 187 2869
Fragments arranged by size:
2869 2004 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5170
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5170
BssSI C'ACGA_G
Cuts at: 3230 4242 3230
Size: 1012 4158
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5170
BstBI TT'CG_AA
Cuts at: 3974 3974
Size: 5170
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5170
BstYI r'GATC_y
Cuts at: 1972 2500 2867 3252 3269 4721 4807 4819 5165
Size: 528 367 385 17 1452 86 12 346
Cuts at: 5165 1972
Size: 1977
Fragments arranged by size:
1977 1452 528 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2333 2333
Size: 5170
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3679
Fragments arranged by size:
3679 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4431
Fragments arranged by size:
4431 683 56
BtsI GCAGTG_nn'
Cuts at: 2686 3480 3500 2686
Size: 794 20 4356
Fragments arranged by size:
4356 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5170
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5135
DraI TTT'AAA
Cuts at: 2280 2474 2280
Size: 194 4976
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4177 809
Size: 507 2861 1802
Fragments arranged by size:
2861 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2617 3506 5135
Size: 54 1425 50 275 18 787 889 1629
Cuts at: 5135 8
Size: 43
Fragments arranged by size:
1629 1425 889 787 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2617 1537
Size: 275 805 4090
Fragments arranged by size:
4090 805 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 3100 1353
Size: 825 922 3423
Fragments arranged by size:
3423 922 825
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3757 4131 4277 387
Size: 1064 138 2168 374 146 1280
Fragments arranged by size:
2168 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3233 3830 4617 4885 1239
Size: 393 1601 597 787 268 1524
Fragments arranged by size:
1601 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2357
Size: 5170
EcoRI G'AATT_C
Cuts at: 2479 2857 2479
Size: 378 4792
FspI TGC'GCA
Cuts at: 3676 3676
Size: 5170
HaeII r_GCGC'y
Cuts at: 1678 4317 1678
Size: 2639 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 3811 3843 3885
Size: 32 135 32 632 32 1575 32 42
Cuts at: 3885 3917 1373
Size: 32 2626
Fragments arranged by size:
2626 1575 632 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 2514 2760 669
Size: 988 281 412 164 246 3079
Fragments arranged by size:
3079 988 412 281 246 164
HindIII A'AGCT_T
Cuts at: 748 2311 748
Size: 1563 3607
HpaI GTT'AAC
Cuts at: 2760 2760
Size: 5170
KpnI G_GTAC'C
Cuts at: 2495 2495
Size: 5170
MfeI C'AATT_G
Cuts at: 2769 2769
Size: 5170
MluI A'CGCG_T
Cuts at: 5046 5046
Size: 5170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4284 4468 1305
Size: 2979 184 2007
Fragments arranged by size:
2979 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3348
Fragments arranged by size:
3348 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3128 3487 3646 518
Size: 648 1962 359 159 2042
Fragments arranged by size:
2042 1962 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2586 3263 4411 4656 1606
Size: 461 519 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 519 461 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3679
Fragments arranged by size:
3679 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5170
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5170
NotI GC'GGCC_GC
Cuts at: 2617 2617
Size: 5170
NspI r_CATG'y
Cuts at: 1132 1229 2398 2528 4073 1132
Size: 97 1169 130 1545 2229
Fragments arranged by size:
2229 1545 1169 130 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5170
PciI A'CATG_T
Cuts at: 4069 4069
Size: 5170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5170
PmeI GTTT'AAAC
Cuts at: 2474 2474
Size: 5170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 4752 4784 3239
Size: 32 1481 32 3625
Fragments arranged by size:
3625 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5170
PsiI TTA'TAA
Cuts at: 2740 2740
Size: 5170
PstI C_TGCA'G
Cuts at: 830 2522 830
Size: 1692 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5170
SalI G'TCGA_C
Cuts at: 1936 2512 1936
Size: 576 4594
SapI GCTCTTCn'nnn_
Cuts at: 2178 2178
Size: 5170
SbfI CC_TGCA'GG
Cuts at: 2522 2522
Size: 5170
ScaI AGT'ACT
Cuts at: 3418 5130 3418
Size: 1712 3458
SfcI C'TryA_G
Cuts at: 826 1045 2223 2518 3653 4334 4525 826
Size: 219 1178 295 1135 681 191 1471
Fragments arranged by size:
1471 1178 1135 681 295 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5170
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5170
SmaI CCC'GGG
Cuts at: 1872 2497 1872
Size: 625 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3274 4175 4437 820
Size: 197 926 1331 901 262 1553
Fragments arranged by size:
1553 1331 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2961 493
Size: 2468 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5170
SphI G_CATG'C
Cuts at: 1132 2528 1132
Size: 1396 3774
SspI AAT'ATT
Cuts at: 5 52 3094 5
Size: 47 3042 2081
Fragments arranged by size:
3042 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5170
StyI C'CwwG_G
Cuts at: 513 1065 2004 2609 513
Size: 552 939 605 3074
Fragments arranged by size:
3074 939 605 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3197 3214 3367 3552 3997 325
Size: 1255 1617 17 153 185 445 1498
Fragments arranged by size:
1617 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3416 5128 96
Size: 274 80 33 51 1443 1439 1712 138
Fragments arranged by size:
1712 1443 1439 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5130 5162 5130
Size: 32 5138
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5170
XbaI T'CTAG_A
Cuts at: 2506 2506
Size: 5170
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5170
XmaI C'CCGG_G
Cuts at: 1870 2495 1870
Size: 625 4545
XmnI GAAnn'nnTTC
Cuts at: 3299 3299
Size: 5170
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2978 276
Size: 53 83 186 936 1444 2468
Fragments arranged by size:
2468 1444 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI