Restriction map for
pFN21AB7525
(Circular) (Six-base) MAPSORT of: pFN21AB7525.seq Check: 9979 from: 1 to: 5155
pFN21AB7525 5155 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2965 278
Size: 53 83 186 936 1429 2468
Fragments arranged by size:
2468 1429 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 2498 1937
Size: 217 344 4594
Fragments arranged by size:
4594 344 217
Acc65I G'GTAC_C
Cuts at: 2207 2476 2207
Size: 269 4886
AclI AA'CG_TT
Cuts at: 1563 3282 3655 1563
Size: 1719 373 3063
Fragments arranged by size:
3063 1719 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2343 3218 4602 4870 1632
Size: 711 875 1384 268 1917
Fragments arranged by size:
1917 1384 875 711 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4958
AflIII A'CryG_T
Cuts at: 1203 2349 4054 5031 1203
Size: 1146 1705 977 1327
Fragments arranged by size:
1705 1327 1146 977
AgeI A'CCGG_T
Cuts at: 3986 3986
Size: 5155
AhdI GACnn_n'nnGTC
Cuts at: 2077 3884 2077
Size: 1807 3348
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2403 1184
Size: 1219 3936
AlwNI CAG_nnn'CTG
Cuts at: 2898 4470 2898
Size: 1572 3583
ApaLI G'TGCA_C
Cuts at: 3212 4368 3212
Size: 1156 3999
ApoI r'AATT_y
Cuts at: 1493 2142 2464 2690 2842 4951 1493
Size: 649 322 226 152 2109 1697
Fragments arranged by size:
2109 1697 649 322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3709 160
Size: 872 2677 1606
Fragments arranged by size:
2677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5155
AvaI C'yCGr_G
Cuts at: 1870 1943 2480 1870
Size: 73 537 4545
Fragments arranged by size:
4545 537 73
BamHI G'GATC_C
Cuts at: 2485 2852 2485
Size: 367 4788
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2476 3931 618
Size: 325 217 627 420 269 1455 1842
Fragments arranged by size:
1842 1455 627 420 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2474 1391
Size: 1083 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3346 3380 1903
Size: 34 1409 34 3678
Fragments arranged by size:
3678 1409 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3047 4257 3047
Size: 1210 3945
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5155
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3766 136
Size: 107 122 71 631 605 2094 1525
Fragments arranged by size:
2094 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5150 5150
Size: 5155
BlpI GC'TnA_GC
Cuts at: 2572 2572
Size: 5155
Bme1580I G_kGCm'C
Cuts at: 1790 3216 4372 1790
Size: 1426 1156 2573
Fragments arranged by size:
2573 1426 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2886 3844 442
Size: 416 53 83 399 24 1469 958 1753
Fragments arranged by size:
1753 1469 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2450 1056
Size: 1394 3761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2447 2434
Size: 13 5142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2404 3815 1239
Size: 1165 1411 2579
Fragments arranged by size:
2579 1411 1165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3280 4145 4443 3280
Size: 865 298 3992
Fragments arranged by size:
3992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3818 882
Size: 617 2319 2219
Fragments arranged by size:
2319 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2350 2946 493
Size: 711 1146 596 2702
Fragments arranged by size:
2702 1146 711 596
BsaBI GATnn'nnATC
Cuts at: 5149 5149
Size: 5155
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2962 3344 3979 275
Size: 53 83 186 936 1429 382 635 1451
Fragments arranged by size:
1451 1429 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3588 3986 4260 4407 4818 3588
Size: 398 274 147 411 3925
Fragments arranged by size:
3925 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4930
Fragments arranged by size:
4930 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5155
BseYI C'CCAG_C
Cuts at: 4358 4854 4358
Size: 496 4659
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4582
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2605 3366 4394 1540
Size: 275 186 604 761 1028 2301
Fragments arranged by size:
2301 1028 761 604 275 186
BsiHKAI G_wGCw'C
Cuts at: 3216 3301 4372 3216
Size: 85 1071 3999
Fragments arranged by size:
3999 1071 85
BsiWI C'GTAC_G
Cuts at: 2346 2346
Size: 5155
BsmI GAATG_Cn'
Cuts at: 2666 2759 2666
Size: 93 5062
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2474 3216 3301 4372 1391
Size: 399 684 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 399 85
BspEI T'CCGG_A
Cuts at: 4818 4818
Size: 5155
BspHI T'CATG_A
Cuts at: 2370 3044 4774 2370
Size: 674 1730 2751
Fragments arranged by size:
2751 1730 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 2510 844
Size: 1562 104 3489
Fragments arranged by size:
3489 1562 104
BsrBI CCG'CTC
Cuts at: 1394 1823 3042 1394
Size: 429 1219 3507
Fragments arranged by size:
3507 1219 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3650 3824 65
Size: 1183 2402 174 1396
Fragments arranged by size:
2402 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3799 3986 1700
Size: 110 1989 187 2869
Fragments arranged by size:
2869 1989 187 110
BsrGI T'GTAC_A
Cuts at: 96 2292 96
Size: 2196 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5155
BssSI C'ACGA_G
Cuts at: 3215 4227 3215
Size: 1012 4143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5155
BstBI TT'CG_AA
Cuts at: 3959 3959
Size: 5155
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5155
BstYI r'GATC_y
Cuts at: 1972 2116 2485 2852 3237 3254 4706 4792 4804
Size: 144 369 367 385 17 1452 86 12
Cuts at: 4804 5150 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 144 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3664
Fragments arranged by size:
3664 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4416
Fragments arranged by size:
4416 683 56
BtsI GCAGTG_nn'
Cuts at: 2671 3465 3485 2671
Size: 794 20 4341
Fragments arranged by size:
4341 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5120
DraI TTT'AAA
Cuts at: 2459 2459
Size: 5155
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4162 809
Size: 507 2846 1802
Fragments arranged by size:
2846 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2602 3491 5120
Size: 54 1425 50 275 18 772 889 1629
Cuts at: 5120 8
Size: 43
Fragments arranged by size:
1629 1425 889 772 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2602 1537
Size: 275 790 4090
Fragments arranged by size:
4090 790 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3085 1353
Size: 1732 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3742 4116 4262 387
Size: 1064 138 2153 374 146 1280
Fragments arranged by size:
2153 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2404 3218 3815 4602 4870 1239
Size: 393 711 61 814 597 787 268 1524
Fragments arranged by size:
1524 814 787 711 597 393 268 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2021 2021
Size: 5155
EcoO109I rG'GnC_Cy
Cuts at: 2362 2362
Size: 5155
EcoRI G'AATT_C
Cuts at: 2464 2842 2464
Size: 378 4777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 5155
FspI TGC'GCA
Cuts at: 3661 3661
Size: 5155
HaeII r_GCGC'y
Cuts at: 1678 4302 1678
Size: 2624 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2087 2095 2119 3796
Size: 32 135 32 491 24 8 24 1677
Cuts at: 3796 3828 3870 3902 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1677 491 135 42 32 32 32
32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2499 2745 669
Size: 988 281 561 246 3079
Fragments arranged by size:
3079 988 561 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5155
HpaI GTT'AAC
Cuts at: 2745 2745
Size: 5155
KpnI G_GTAC'C
Cuts at: 2211 2480 2211
Size: 269 4886
MfeI C'AATT_G
Cuts at: 2754 2754
Size: 5155
MluI A'CGCG_T
Cuts at: 5031 5031
Size: 5155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4269 4453 1305
Size: 2964 184 2007
Fragments arranged by size:
2964 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3333
Fragments arranged by size:
3333 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3113 3472 3631 518
Size: 648 1947 359 159 2042
Fragments arranged by size:
2042 1947 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2571 3248 4396 4641 1606
Size: 965 677 1148 245 2120
Fragments arranged by size:
2120 1148 965 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3664
Fragments arranged by size:
3664 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5155
NheI G'CTAG_C
Cuts at: 1052 2446 1052
Size: 1394 3761
NotI GC'GGCC_GC
Cuts at: 2602 2602
Size: 5155
NspI r_CATG'y
Cuts at: 1132 1229 2513 4058 1132
Size: 97 1284 1545 2229
Fragments arranged by size:
2229 1545 1284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5155
PciI A'CATG_T
Cuts at: 4054 4054
Size: 5155
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5155
PmeI GTTT'AAAC
Cuts at: 2459 2459
Size: 5155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 4737 4769 3224
Size: 32 1481 32 3610
Fragments arranged by size:
3610 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5155
PsiI TTA'TAA
Cuts at: 2725 2725
Size: 5155
PstI C_TGCA'G
Cuts at: 830 2382 2507 830
Size: 1552 125 3478
Fragments arranged by size:
3478 1552 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5155
SalI G'TCGA_C
Cuts at: 1936 2497 1936
Size: 561 4594
SbfI CC_TGCA'GG
Cuts at: 2507 2507
Size: 5155
ScaI AGT'ACT
Cuts at: 3403 5115 3403
Size: 1712 3443
SfcI C'TryA_G
Cuts at: 826 1045 2151 2378 2503 3638 4319 4510 826
Size: 219 1106 227 125 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 681 227 219 191 125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5155
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5155
SmaI CCC'GGG
Cuts at: 1872 2482 1872
Size: 610 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3259 4160 4422 820
Size: 197 926 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2946 493
Size: 2453 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5155
SphI G_CATG'C
Cuts at: 1132 2513 1132
Size: 1381 3774
SspI AAT'ATT
Cuts at: 5 52 3079 5
Size: 47 3027 2081
Fragments arranged by size:
3027 2081 47
StuI AGG'CCT
Cuts at: 1517 2248 1517
Size: 731 4424
StyI C'CwwG_G
Cuts at: 513 1065 2004 2594 513
Size: 552 939 590 3074
Fragments arranged by size:
3074 939 590 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2088 2150 3182 3199 3352 3537 3982
Size: 1255 508 62 1032 17 153 185 445
Cuts at: 3982 325
Size: 1498
Fragments arranged by size:
1498 1255 1032 508 445 185 153 62
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 3401 5113
Size: 274 80 33 51 1443 315 1109 1712
Cuts at: 5113 96
Size: 138
Fragments arranged by size:
1712 1443 1109 315 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 5115 5147 2397
Size: 32 2686 32 2405
Fragments arranged by size:
2686 2405 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5155
XbaI T'CTAG_A
Cuts at: 2491 2491
Size: 5155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5155
XmaI C'CCGG_G
Cuts at: 1870 2480 1870
Size: 610 4545
XmnI GAAnn'nnTTC
Cuts at: 3284 3284
Size: 5155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2963 276
Size: 53 83 186 936 1429 2468
Fragments arranged by size:
2468 1429 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI