Restriction map for
pFN21AB7520
(Circular) (Six-base) MAPSORT of: pFN21AB7520.seq Check: 456 from: 1 to: 5449
pFN21AB7520 5449 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 3512 4896 5164 1632
Size: 440 1440 1384 268 1917
Fragments arranged by size:
1917 1440 1384 440 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 2009 4348 5325 1203
Size: 806 2339 977 1327
Fragments arranged by size:
2339 1327 977 806
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 3192 4764 3192
Size: 1572 3877
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2152 2758 2984 3136 5245 1493
Size: 659 606 226 152 2109 1697
Fragments arranged by size:
2109 1697 659 606 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2774 1870
Size: 73 831 4545
Fragments arranged by size:
4545 831 73
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2770 4225 618
Size: 325 217 627 983 1455 1842
Fragments arranged by size:
1842 1455 983 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2768 1391
Size: 1377 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2495 928
Size: 971 596 3882
Fragments arranged by size:
3882 971 596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 2662 1548
Size: 1114 4335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4060 136
Size: 107 122 71 631 605 2388 1525
Fragments arranged by size:
2388 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2083 2634 5444 2083
Size: 551 2810 2088
Fragments arranged by size:
2810 2088 551
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2521 3510 4666 1790
Size: 731 989 1156 2573
Fragments arranged by size:
2573 1156 989 731
BmgBI CAC'GTC
Cuts at: 1318 2010 1318
Size: 692 4757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2288 4109 1239
Size: 861 188 1821 2579
Fragments arranged by size:
2579 1821 861 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3574 4439 4737 3574
Size: 865 298 4286
Fragments arranged by size:
4286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 3638 4273 275
Size: 53 83 186 936 1723 382 635 1451
Fragments arranged by size:
1723 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2374 2559 3882 4280 4554 4701 5112 2374
Size: 185 1323 398 274 147 411 2711
Fragments arranged by size:
2711 1323 411 398 274 185 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5224
Fragments arranged by size:
5224 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 1186
Size: 1012 4437
BseYI C'CCAG_C
Cuts at: 2029 4652 5148 2029
Size: 2623 496 2330
Fragments arranged by size:
2623 2330 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4876
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3510 3595 4666 3510
Size: 85 1071 4293
Fragments arranged by size:
4293 1071 85
BsmI GAATG_Cn'
Cuts at: 2698 2960 3053 2698
Size: 262 93 5094
Fragments arranged by size:
5094 262 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2521 2768 3510 3595 4666 1391
Size: 399 731 247 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 731 399 247 85
BspEI T'CCGG_A
Cuts at: 2374 2559 5112 2374
Size: 185 2553 2711
Fragments arranged by size:
2711 2553 185
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 844
Size: 1960 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 4280 1700
Size: 110 2283 187 2869
Fragments arranged by size:
2869 2283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2083 2634 2779 3146 3531 3548 5000 5086
Size: 111 551 145 367 385 17 1452 86
Cuts at: 5086 5098 5444 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 551 385 367 346 145 111
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4897
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3759 3779 2965
Size: 794 20 4635
Fragments arranged by size:
4635 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2682 2753 2682
Size: 71 5378
DraIII CAC_nnn'GTG
Cuts at: 2651 2651
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2511 2896 3785
Size: 54 1425 50 275 18 681 385 889
Cuts at: 3785 5414 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 681 385 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2304 3379 1353
Size: 951 1075 3423
Fragments arranged by size:
3423 1075 951
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4036 4410 4556 387
Size: 1064 138 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2100 2288 3512 4109 4896 5164
Size: 393 440 28 188 1224 597 787 268
Cuts at: 5164 1239
Size: 1524
Fragments arranged by size:
1524 1224 787 597 440 393 268 188
28
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2468 2468
Size: 5449
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 4596 1678
Size: 2918 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 4090 4122 4164
Size: 32 135 32 797 32 1689 32 42
Cuts at: 4164 4196 1373
Size: 32 2626
Fragments arranged by size:
2626 1689 797 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2362 4563 4747 1305
Size: 1057 2201 184 2007
Fragments arranged by size:
2201 2007 1057 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 10
Size: 54 1425 343 681 2946
Fragments arranged by size:
2946 1425 681 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2284 2865 3542 4690 4935 1606
Size: 554 124 581 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 581 554 245 124
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4897
NdeI CA'TA_TG
Cuts at: 387 2713 387
Size: 2326 3123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2807 4352 1132
Size: 97 1578 1545 2229
Fragments arranged by size:
2229 1578 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2509 1108
Size: 1401 4048
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 2703 3019 2703
Size: 316 5133
PstI C_TGCA'G
Cuts at: 830 2265 2801 830
Size: 1435 536 3478
Fragments arranged by size:
3478 1435 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
PvuII CAG'CTG
Cuts at: 2160 2284 2160
Size: 124 5325
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 2093 3697 5409 2093
Size: 1604 1712 2133
Fragments arranged by size:
2133 1712 1604
SfcI C'TryA_G
Cuts at: 826 1045 2178 2261 2797 3932 4613 4804 826
Size: 219 1133 83 536 1135 681 191 1471
Fragments arranged by size:
1471 1135 1133 681 536 219 191 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3553 4454 4716 820
Size: 197 926 1610 901 262 1553
Fragments arranged by size:
1610 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 2248 3373 5
Size: 47 2196 1125 2081
Fragments arranged by size:
2196 2081 1125 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2888 513
Size: 552 1823 3074
Fragments arranged by size:
3074 1823 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 3695 5407
Size: 274 80 33 51 1443 114 1604 1712
Cuts at: 5407 96
Size: 138
Fragments arranged by size:
1712 1604 1443 274 138 114 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5409 5441 5409
Size: 32 5417
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 2352 3578 2352
Size: 1226 4223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI