Restriction map for
pFN21AB7518
(Circular) (Six-base) MAPSORT of: pFN21AB7518.seq Check: 9276 from: 1 to: 5443
pFN21AB7518 5443 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2723 3506 4890 5158 1632
Size: 1091 783 1384 268 1917
Fragments arranged by size:
1917 1384 1091 783 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 2135 2530 4342 5319 1203
Size: 932 395 1812 977 1327
Fragments arranged by size:
1812 1327 977 932 395
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2286 1184
Size: 1102 4341
AlwNI CAG_nnn'CTG
Cuts at: 2654 3186 4758 2654
Size: 532 1572 3339
Fragments arranged by size:
3339 1572 532
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2040 2564 2576 2752 2978 3130 5239 1493
Size: 547 524 12 176 226 152 2109 1697
Fragments arranged by size:
2109 1697 547 524 226 176 152 12
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 1870
Size: 73 825 4545
Fragments arranged by size:
4545 825 73
BamHI G'GATC_C
Cuts at: 2064 2773 3140 2064
Size: 709 367 4367
Fragments arranged by size:
4367 709 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2764 4219 618
Size: 325 217 627 539 438 1455 1842
Fragments arranged by size:
1842 1455 627 539 438 325 217
BanII G_rGCy'C
Cuts at: 1391 2232 2762 1391
Size: 841 530 4072
Fragments arranged by size:
4072 841 530
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4054 136
Size: 107 122 71 631 605 337 2045 1525
Fragments arranged by size:
2045 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 3504 4660 1790
Size: 1714 1156 2573
Fragments arranged by size:
2573 1714 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 2616 4103 1239
Size: 1092 285 1487 2579
Fragments arranged by size:
2579 1487 1092 285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2208 3568 4433 4731 2208
Size: 1360 865 298 2920
Fragments arranged by size:
2920 1360 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 4106 882
Size: 617 1170 1437 2219
Fragments arranged by size:
2219 1437 1170 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3250 3632 4267 275
Size: 53 83 186 936 1717 382 635 1451
Fragments arranged by size:
1717 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3876 4274 4548 4695 5106 3876
Size: 398 274 147 411 4213
Fragments arranged by size:
4213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2681 1186
Size: 1495 3948
BseYI C'CCAG_C
Cuts at: 2035 4646 5142 2035
Size: 2611 496 2336
Fragments arranged by size:
2611 2336 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 1163
Size: 573 867 4003
Fragments arranged by size:
4003 867 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3504 3589 4660 3504
Size: 85 1071 4287
Fragments arranged by size:
4287 1071 85
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2762 3504 3589 4660 1391
Size: 399 442 530 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 442 399 85
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 2458 3332 5062 2458
Size: 874 1730 2839
Fragments arranged by size:
2839 1730 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 844
Size: 1954 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2304 3938 4112 65
Size: 1183 1056 1634 174 1396
Fragments arranged by size:
1634 1396 1183 1056 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 2689 96
Size: 2593 2850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2064 2773 3140 3525 3542 4994 5080 5092
Size: 92 709 367 385 17 1452 86 12
Cuts at: 5092 5438 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 709 385 367 346 92 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2664 2664
Size: 5443
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3952
Fragments arranged by size:
3952 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2376 2959 3753 3773 2376
Size: 583 794 20 4046
Fragments arranged by size:
4046 794 583 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2890 3779 5408
Size: 54 1425 50 275 18 1060 889 1629
Cuts at: 5408 8
Size: 43
Fragments arranged by size:
1629 1425 1060 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 3373 1353
Size: 1104 916 3423
Fragments arranged by size:
3423 1104 916
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 4404 4550 387
Size: 1064 138 2441 374 146 1280
Fragments arranged by size:
2441 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2616 2723 3506 4103 4890 5158
Size: 393 699 285 107 783 597 787 268
Cuts at: 5158 1239
Size: 1524
Fragments arranged by size:
1524 787 783 699 597 393 285 268
107
EcoNI CCTnn'n_nnAGG
Cuts at: 2430 2430
Size: 5443
EcoO109I rG'GnC_Cy
Cuts at: 2714 2714
Size: 5443
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
EcoRV GAT'ATC
Cuts at: 2020 2420 2020
Size: 400 5043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2136 2136
Size: 5443
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 4590 1678
Size: 2912 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 4084 4116 4158
Size: 32 135 32 752 32 1728 32 42
Cuts at: 4158 4190 1373
Size: 32 2626
Fragments arranged by size:
2626 1728 752 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2787 3033 669
Size: 988 281 115 734 246 3079
Fragments arranged by size:
3079 988 734 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2176 4557 4741 1305
Size: 871 2381 184 2007
Fragments arranged by size:
2381 2007 871 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3621
Fragments arranged by size:
3621 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2260 2859 3536 4684 4929 1606
Size: 654 599 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 654 599 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3952
Fragments arranged by size:
3952 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2139 2222 2801 4346 1132
Size: 97 910 83 579 1545 2229
Fragments arranged by size:
2229 1545 910 579 97 83
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 2135 4342 2135
Size: 2207 3236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5443
PfoI T'CCnGG_A
Cuts at: 2091 2091
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 2348 3013 2348
Size: 665 4778
PstI C_TGCA'G
Cuts at: 830 2371 2795 830
Size: 1541 424 3478
Fragments arranged by size:
3478 1541 424
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SapI GCTCTTCn'nnn_
Cuts at: 2457 2457
Size: 5443
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2367 2791 3926 4607 4798 826
Size: 219 1322 424 1135 681 191 1471
Fragments arranged by size:
1471 1322 1135 681 424 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2770 1872
Size: 898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2223 3547 4448 4710 820
Size: 197 926 280 1324 901 262 1553
Fragments arranged by size:
1553 1324 926 901 280 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2222 2801 1132
Size: 1090 579 3774
Fragments arranged by size:
3774 1090 579
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2004 2882 513
Size: 552 939 878 3074
Fragments arranged by size:
3074 939 878 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2587 3470 3487 3640 3825 4270 325
Size: 1255 1007 883 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1007 883 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 2689 3689
Size: 274 80 33 51 1443 228 484 1000
Cuts at: 3689 5401 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1000 484 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2354 2779 2354
Size: 425 5018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2768 1870
Size: 898 4545
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI