Restriction map for
pFN21AB7513
(Circular) (Six-base) MAPSORT of: pFN21AB7513.seq Check: 7348 from: 1 to: 5080
pFN21AB7513 5080 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2890 278
Size: 53 83 186 936 1354 2468
Fragments arranged by size:
2468 1354 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2423 1937
Size: 486 4594
Acc65I G'GTAC_C
Cuts at: 2401 2401
Size: 5080
AclI AA'CG_TT
Cuts at: 1563 3207 3580 1563
Size: 1644 373 3063
Fragments arranged by size:
3063 1644 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3143 4527 4795 1632
Size: 1511 1384 268 1917
Fragments arranged by size:
1917 1511 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4883
AflIII A'CryG_T
Cuts at: 1203 2115 2217 2340 3979 4956 1203
Size: 912 102 123 1639 977 1327
Fragments arranged by size:
1639 1327 977 912 123 102
AgeI A'CCGG_T
Cuts at: 3911 3911
Size: 5080
AhdI GACnn_n'nnGTC
Cuts at: 3809 3809
Size: 5080
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5080
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5080
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 2273
Size: 32 5048
AlwNI CAG_nnn'CTG
Cuts at: 2823 4395 2823
Size: 1572 3508
ApaLI G'TGCA_C
Cuts at: 2344 3137 4293 2344
Size: 793 1156 3131
Fragments arranged by size:
3131 1156 793
ApoI r'AATT_y
Cuts at: 1493 2389 2615 2767 4876 1493
Size: 896 226 152 2109 1697
Fragments arranged by size:
2109 1697 896 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3634 160
Size: 872 2602 1606
Fragments arranged by size:
2602 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5080
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 1870
Size: 73 462 4545
Fragments arranged by size:
4545 462 73
BamHI G'GATC_C
Cuts at: 2410 2777 2410
Size: 367 4713
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2401 3856 618
Size: 325 217 627 614 1455 1842
Fragments arranged by size:
1842 1455 627 614 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2399 1391
Size: 622 386 4072
Fragments arranged by size:
4072 622 386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2094 928
Size: 971 195 3914
Fragments arranged by size:
3914 971 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3271 3305 1903
Size: 34 1334 34 3678
Fragments arranged by size:
3678 1334 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2972 4182 2972
Size: 1210 3870
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5080
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3691 136
Size: 107 122 71 631 605 2019 1525
Fragments arranged by size:
2019 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5075 5075
Size: 5080
BlpI GC'TnA_GC
Cuts at: 2497 2497
Size: 5080
Bme1580I G_kGCm'C
Cuts at: 1790 2348 3141 4297 1790
Size: 558 793 1156 2573
Fragments arranged by size:
2573 1156 793 558
BmgBI CAC'GTC
Cuts at: 1318 2218 1318
Size: 900 4180
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2811 3769 442
Size: 416 53 83 399 24 1394 958 1753
Fragments arranged by size:
1753 1394 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3740 1239
Size: 2501 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3205 4070 4368 3205
Size: 865 298 3917
Fragments arranged by size:
3917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3743 882
Size: 617 2244 2219
Fragments arranged by size:
2244 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2341 2871 493
Size: 711 1137 530 2702
Fragments arranged by size:
2702 1137 711 530
BsaBI GATnn'nnATC
Cuts at: 5074 5074
Size: 5080
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 2887 3269 3904
Size: 53 83 186 936 789 565 382 635
Cuts at: 3904 275
Size: 1451
Fragments arranged by size:
1451 936 789 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3513 3911 4185 4332 4743 3513
Size: 398 274 147 411 3850
Fragments arranged by size:
3850 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 1178
Size: 30 165 30 870 30 3955
Fragments arranged by size:
3955 870 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5080
BseYI C'CCAG_C
Cuts at: 4283 4779 4283
Size: 496 4584
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2530 3291 4319 1540
Size: 275 186 529 761 1028 2301
Fragments arranged by size:
2301 1028 761 529 275 186
BsiHKAI G_wGCw'C
Cuts at: 2348 3141 3226 4297 2348
Size: 793 85 1071 3131
Fragments arranged by size:
3131 1071 793 85
BsmI GAATG_Cn'
Cuts at: 2103 2591 2684 2103
Size: 488 93 4499
Fragments arranged by size:
4499 488 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2348 2399 3141 3226 4297 1391
Size: 399 223 335 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 223 85 51
BspEI T'CCGG_A
Cuts at: 4743 4743
Size: 5080
BspHI T'CATG_A
Cuts at: 2969 4699 2969
Size: 1730 3350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 844
Size: 1591 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2967 1394
Size: 429 1144 3507
Fragments arranged by size:
3507 1144 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3575 3749 65
Size: 1183 2327 174 1396
Fragments arranged by size:
2327 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3724 3911 1700
Size: 110 1914 187 2869
Fragments arranged by size:
2869 1914 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5080
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5080
BssSI C'ACGA_G
Cuts at: 3140 4152 3140
Size: 1012 4068
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5080
BstBI TT'CG_AA
Cuts at: 3884 3884
Size: 5080
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5080
BstYI r'GATC_y
Cuts at: 1972 2410 2777 3162 3179 4631 4717 4729 5075
Size: 438 367 385 17 1452 86 12 346
Cuts at: 5075 1972
Size: 1977
Fragments arranged by size:
1977 1452 438 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2240 513
Size: 552 939 236 3353
Fragments arranged by size:
3353 939 552 236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4341
Fragments arranged by size:
4341 683 56
BtsI GCAGTG_nn'
Cuts at: 2356 2596 3390 3410 2356
Size: 240 794 20 4026
Fragments arranged by size:
4026 794 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5080
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5045
DraI TTT'AAA
Cuts at: 2384 2384
Size: 5080
DraIII CAC_nnn'GTG
Cuts at: 2188 2306 2188
Size: 118 4962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4087 809
Size: 507 2771 1802
Fragments arranged by size:
2771 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2272 2362 2527
Size: 54 1425 50 275 18 442 90 165
Cuts at: 2527 3416 5045 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 442 275 165 90 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2527 1537
Size: 275 715 4090
Fragments arranged by size:
4090 715 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3010 1353
Size: 1657 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3667 4041 4187 387
Size: 1064 138 2078 374 146 1280
Fragments arranged by size:
2078 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3143 3740 4527 4795 1239
Size: 393 1511 597 787 268 1524
Fragments arranged by size:
1524 1511 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2227 2227
Size: 5080
EcoRI G'AATT_C
Cuts at: 2389 2767 2389
Size: 378 4702
FspI TGC'GCA
Cuts at: 3586 3586
Size: 5080
HaeII r_GCGC'y
Cuts at: 1678 4227 1678
Size: 2549 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3721 3753 3795 3827 1373
Size: 32 135 32 2149 32 42 32 2626
Fragments arranged by size:
2626 2149 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 2424 2670 669
Size: 988 281 232 254 246 3079
Fragments arranged by size:
3079 988 281 254 246 232
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5080
HpaI GTT'AAC
Cuts at: 2670 2670
Size: 5080
KpnI G_GTAC'C
Cuts at: 2405 2405
Size: 5080
MfeI C'AATT_G
Cuts at: 2679 2679
Size: 5080
MluI A'CGCG_T
Cuts at: 2115 4956 2115
Size: 2841 2239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4194 4378 1305
Size: 2889 184 2007
Fragments arranged by size:
2889 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3258
Fragments arranged by size:
3258 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3038 3397 3556 518
Size: 648 1872 359 159 2042
Fragments arranged by size:
2042 1872 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2242 2370 2496 3173 4321 4566 1606
Size: 551 85 128 126 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 551 245 128 126 85
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3589
Fragments arranged by size:
3589 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5080
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5080
NotI GC'GGCC_GC
Cuts at: 2527 2527
Size: 5080
NspI r_CATG'y
Cuts at: 1132 1229 2074 2438 3983 1132
Size: 97 845 364 1545 2229
Fragments arranged by size:
2229 1545 845 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5080
PciI A'CATG_T
Cuts at: 3979 3979
Size: 5080
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5080
PmeI GTTT'AAAC
Cuts at: 2384 2384
Size: 5080
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2273 2305 3149 3181 4662 4694 2273
Size: 32 844 32 1481 32 2659
Fragments arranged by size:
2659 1481 844 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 2227
Size: 5080
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5080
PsiI TTA'TAA
Cuts at: 2650 2650
Size: 5080
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2117 2149 2117
Size: 32 5048
PstI C_TGCA'G
Cuts at: 830 2432 830
Size: 1602 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5080
PvuII CAG'CTG
Cuts at: 2157 2157
Size: 5080
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 5080
SacII CC_GC'GG
Cuts at: 2243 2243
Size: 5080
SalI G'TCGA_C
Cuts at: 1936 2422 1936
Size: 486 4594
SbfI CC_TGCA'GG
Cuts at: 2432 2432
Size: 5080
ScaI AGT'ACT
Cuts at: 3328 5040 3328
Size: 1712 3368
SfcI C'TryA_G
Cuts at: 826 1045 2428 3563 4244 4435 826
Size: 219 1383 1135 681 191 1471
Fragments arranged by size:
1471 1383 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5080
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5080
SmaI CCC'GGG
Cuts at: 1872 2407 1872
Size: 535 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3184 4085 4347 820
Size: 197 926 1241 901 262 1553
Fragments arranged by size:
1553 1241 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2871 493
Size: 2378 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5080
SphI G_CATG'C
Cuts at: 1132 2438 1132
Size: 1306 3774
SspI AAT'ATT
Cuts at: 5 52 3004 5
Size: 47 2952 2081
Fragments arranged by size:
2952 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5080
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2519 513
Size: 552 939 78 437 3074
Fragments arranged by size:
3074 939 552 437 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 2390 3107 3124 3277 3462 3907
Size: 1255 681 129 717 17 153 185 445
Cuts at: 3907 325
Size: 1498
Fragments arranged by size:
1498 1255 717 681 445 185 153 129
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3326 5038 96
Size: 274 80 33 51 1443 1349 1712 138
Fragments arranged by size:
1712 1443 1349 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 5040 5072 2243
Size: 32 2765 32 2251
Fragments arranged by size:
2765 2251 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5080
XbaI T'CTAG_A
Cuts at: 2416 2416
Size: 5080
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5080
XmaI C'CCGG_G
Cuts at: 1870 2405 1870
Size: 535 4545
XmnI GAAnn'nnTTC
Cuts at: 3209 3209
Size: 5080
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2888 276
Size: 53 83 186 936 1354 2468
Fragments arranged by size:
2468 1354 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII RsrII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI