Restriction map for
pFN21AB7510
(Circular) (Six-base) MAPSORT of: pFN21AB7510.seq Check: 5968 from: 1 to: 5431
pFN21AB7510 5431 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2282 2752 2282
Size: 470 4961
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2029 2363 3494 4878 5146 1632
Size: 397 334 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 397 334 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 4330 5307 1203
Size: 3127 977 1327
Fragments arranged by size:
3127 1327 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 2131 3174 4746 2131
Size: 1043 1572 2816
Fragments arranged by size:
2816 1572 1043
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2477 2740 2966 3118 5227 1493
Size: 984 263 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 1870
Size: 73 813 4545
Fragments arranged by size:
4545 813 73
BamHI G'GATC_C
Cuts at: 2761 3128 2761
Size: 367 5064
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2282 2752 4207 618
Size: 325 217 627 291 204 470 1455 1842
Fragments arranged by size:
1842 1455 627 470 325 291 217 204
BanII G_rGCy'C
Cuts at: 1391 2750 1391
Size: 1359 4072
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 5431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 928
Size: 971 147 4313
Fragments arranged by size:
4313 971 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2516 2550 3622 3656 1903
Size: 34 579 34 1072 34 3678
Fragments arranged by size:
3678 1072 579 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4042 136
Size: 107 122 71 631 605 2370 1525
Fragments arranged by size:
2370 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2848 2848
Size: 5431
Bme1580I G_kGCm'C
Cuts at: 1790 3492 4648 1790
Size: 1702 1156 2573
Fragments arranged by size:
2573 1702 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3162 4120 442
Size: 416 53 83 399 24 1745 958 1753
Fragments arranged by size:
1753 1745 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2388 2526 2699 4091 1239
Size: 902 247 138 173 1392 2579
Fragments arranged by size:
2579 1392 902 247 173 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 3556 4421 4719 2708
Size: 848 865 298 3420
Fragments arranged by size:
3420 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2644 4094 882
Size: 617 1145 1450 2219
Fragments arranged by size:
2219 1450 1145 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 3238 3620 4255
Size: 53 83 186 936 546 1159 382 635
Cuts at: 4255 275
Size: 1451
Fragments arranged by size:
1451 1159 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3864 4262 4536 4683 5094 3864
Size: 398 274 147 411 4201
Fragments arranged by size:
4201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5206
Fragments arranged by size:
5206 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5431
BseYI C'CCAG_C
Cuts at: 2300 4634 5130 2300
Size: 2334 496 2601
Fragments arranged by size:
2601 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4858
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3642 4670 1540
Size: 275 186 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 3492 3577 4648 2106
Size: 1386 85 1071 2889
Fragments arranged by size:
2889 1386 1071 85
BsmI GAATG_Cn'
Cuts at: 2599 2942 3035 2599
Size: 343 93 4995
Fragments arranged by size:
4995 343 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2750 3492 3577 4648 1391
Size: 399 316 644 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 399 316 85
BspEI T'CCGG_A
Cuts at: 5094 5094
Size: 5431
BspHI T'CATG_A
Cuts at: 2257 3320 5050 2257
Size: 1063 1730 2638
Fragments arranged by size:
2638 1730 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2786 844
Size: 1942 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3318 1394
Size: 429 1495 3507
Fragments arranged by size:
3507 1495 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2637 3926 4100 65
Size: 1183 1389 1289 174 1396
Fragments arranged by size:
1396 1389 1289 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 4075 4262 1700
Size: 110 283 1982 187 2869
Fragments arranged by size:
2869 1982 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 794
Size: 1811 3620
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 2614 1184
Size: 1430 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 2547 2586 2547
Size: 39 5392
BstYI r'GATC_y
Cuts at: 1972 2761 3128 3513 3530 4982 5068 5080 5426
Size: 789 367 385 17 1452 86 12 346
Cuts at: 5426 1972
Size: 1977
Fragments arranged by size:
1977 1452 789 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2619 513
Size: 552 939 615 3325
Fragments arranged by size:
3325 939 615 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3741 3761 2947
Size: 794 20 4617
Fragments arranged by size:
4617 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2155 2878
Size: 54 1425 50 275 18 177 148 723
Cuts at: 2878 3767 5396 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 723 275 177 148 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3361 1353
Size: 2008 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2321 4018 4392 4538 387
Size: 1064 138 732 1697 374 146 1280
Fragments arranged by size:
1697 1280 1064 732 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2141 2363 2388 2526 2699 3494
Size: 393 397 112 222 25 138 173 795
Cuts at: 3494 4091 4878 5146 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 397 393 268 222
173 138 112 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2123
Size: 5431
EcoO109I rG'GnC_Cy
Cuts at: 2128 2128
Size: 5431
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 5431
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2082 4578 1678
Size: 404 2496 2531
Fragments arranged by size:
2531 2496 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2474 2506 4072
Size: 32 135 32 653 32 217 32 1566
Cuts at: 4072 4104 4146 4178 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 653 217 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2326 2775 3021 669
Size: 988 281 388 449 246 3079
Fragments arranged by size:
3079 988 449 388 281 246
HindIII A'AGCT_T
Cuts at: 748 2030 2184 748
Size: 1282 154 3995
Fragments arranged by size:
3995 1282 154
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 5431
KpnI G_GTAC'C
Cuts at: 2286 2756 2286
Size: 470 4961
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 2245 4545 4729 1305
Size: 903 37 2300 184 2007
Fragments arranged by size:
2300 2007 903 184 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 3748 3907 518
Size: 648 2223 359 159 2042
Fragments arranged by size:
2223 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2847 3524 4672 4917 1606
Size: 479 762 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 479 245
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 5431
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3940
Fragments arranged by size:
3940 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2789 4334 1132
Size: 97 1560 1545 2229
Fragments arranged by size:
2229 1560 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2701 1108
Size: 1593 3838
PfoI T'CCnGG_A
Cuts at: 2118 2118
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 2190 3001 2190
Size: 811 4620
PstI C_TGCA'G
Cuts at: 830 2353 2783 830
Size: 1523 430 3478
Fragments arranged by size:
3478 1523 430
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SfcI C'TryA_G
Cuts at: 826 1045 2349 2779 3914 4595 4786 826
Size: 219 1304 430 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 681 430 219 191
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 5431
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2758 1872
Size: 886 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 3535 4436 4698 820
Size: 197 926 780 812 901 262 1553
Fragments arranged by size:
1553 926 901 812 780 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 2503 1517
Size: 986 4445
StyI C'CwwG_G
Cuts at: 513 1065 2004 2460 2870 513
Size: 552 939 456 410 3074
Fragments arranged by size:
3074 939 552 456 410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2343 3458 3475 3628 3813 4258 325
Size: 1255 763 1115 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1115 763 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3677 5389 96
Size: 274 80 33 51 1443 1700 1712 138
Fragments arranged by size:
1712 1700 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5391 5423 5391
Size: 32 5399
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2698 2698
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2756 1870
Size: 886 4545
XmnI GAAnn'nnTTC
Cuts at: 3560 3560
Size: 5431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI