Restriction map for
pFN21AB7506
(Circular) (Six-base) MAPSORT of: pFN21AB7506.seq Check: 2079 from: 1 to: 5425
pFN21AB7506 5425 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 3488 4872 5140 1632
Size: 920 936 1384 268 1917
Fragments arranged by size:
1917 1384 936 920 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5425
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2495 2527 2495
Size: 32 5393
AlwNI CAG_nnn'CTG
Cuts at: 2428 3168 4740 2428
Size: 740 1572 3113
Fragments arranged by size:
3113 1572 740
ApaI G_GGCC'C
Cuts at: 2449 2449
Size: 5425
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2539 2734 2960 3112 5221 1493
Size: 1046 195 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 195 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2279 2438 2750 1870
Size: 73 336 159 312 4545
Fragments arranged by size:
4545 336 312 159 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 2746 4201 618
Size: 325 217 627 541 418 1455 1842
Fragments arranged by size:
1842 1455 627 541 418 325 217
BanII G_rGCy'C
Cuts at: 1391 2449 2744 1391
Size: 1058 295 4072
Fragments arranged by size:
4072 1058 295
BbeI G_GCGC'C
Cuts at: 2332 2332
Size: 5425
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2047 928
Size: 971 148 4306
Fragments arranged by size:
4306 971 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 4036 136
Size: 107 122 71 631 605 781 1583 1525
Fragments arranged by size:
1583 1525 781 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2490 5420 2490
Size: 2930 2495
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2449 3486 4642 1790
Size: 659 1037 1156 2573
Fragments arranged by size:
2573 1156 1037 659
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2131 2362 4085 1239
Size: 892 231 1723 2579
Fragments arranged by size:
2579 1723 892 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4088 882
Size: 617 2589 2219
Fragments arranged by size:
2589 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3216 493
Size: 711 2012 2702
Fragments arranged by size:
2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2329 3232 3614
Size: 53 83 186 936 507 289 903 382
Cuts at: 3614 4249 275
Size: 635 1451
Fragments arranged by size:
1451 936 903 635 507 382 289 186
83 53
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 1178
Size: 30 165 30 1092 30 4078
Fragments arranged by size:
4078 1092 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2683 1186
Size: 1497 3928
BseYI C'CCAG_C
Cuts at: 2448 4628 5124 2448
Size: 2180 496 2749
Fragments arranged by size:
2749 2180 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 2875 3636 4664 1540
Size: 275 186 34 840 761 1028 2301
Fragments arranged by size:
2301 1028 840 761 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 2268 3486 3571 4642 2268
Size: 1218 85 1071 3051
Fragments arranged by size:
3051 1218 1071 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2449 2744 3486 3571 4642 1391
Size: 399 478 181 295 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 295 181 85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2266 2780 844
Size: 1422 514 3489
Fragments arranged by size:
3489 1422 514
BsrBI CCG'CTC
Cuts at: 1394 1823 2326 3312 1394
Size: 429 503 986 3507
Fragments arranged by size:
3507 986 503 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3920 4094 65
Size: 1183 2672 174 1396
Fragments arranged by size:
2672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4069 4256 1700
Size: 110 2259 187 2869
Fragments arranged by size:
2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2490 2620 2755 3122 3507 3524 4976 5062
Size: 518 130 135 367 385 17 1452 86
Cuts at: 5062 5074 5420 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 518 385 367 346 135 130
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2271 2334 2271
Size: 63 5362
BtgI C'CryG_G
Cuts at: 513 1065 2229 2300 513
Size: 552 1164 71 3638
Fragments arranged by size:
3638 1164 552 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2158 2872
Size: 54 1425 50 275 18 202 126 714
Cuts at: 2872 3761 5390 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 714 275 202 126 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2032 2872 1537
Size: 275 220 840 4090
Fragments arranged by size:
4090 840 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 3355 1353
Size: 2002 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4012 4386 4532 387
Size: 1064 138 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2131 2362 2552 3488 4085 4872 5140
Size: 393 499 231 190 936 597 787 268
Cuts at: 5140 1239
Size: 1524
Fragments arranged by size:
1524 936 787 597 499 393 268 231
190
EcoNI CCTnn'n_nnAGG
Cuts at: 2146 2146
Size: 5425
EcoO109I rG'GnC_Cy
Cuts at: 2338 2445 2574 2637 2338
Size: 107 129 63 5126
Fragments arranged by size:
5126 129 107 63
EcoRI G'AATT_C
Cuts at: 2539 2734 3112 2539
Size: 195 378 4852
Fragments arranged by size:
4852 378 195
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2237 2332 2435 4572 1678
Size: 559 95 103 2137 2531
Fragments arranged by size:
2531 2137 559 103 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4066 4098 4140 4172 1373
Size: 32 135 32 2494 32 42 32 2626
Fragments arranged by size:
2626 2494 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KasI G'GCGC_C
Cuts at: 2328 2328
Size: 5425
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2160 10
Size: 54 1425 343 328 3275
Fragments arranged by size:
3275 1425 343 328 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3383 3742 3901 518
Size: 648 2217 359 159 2042
Fragments arranged by size:
2217 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 2302 2389 2452 2841 3518 4666 4911
Size: 625 71 87 63 389 677 1148 245
Cuts at: 4911 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 625 389 245 87 71
63
NarI GG'CG_CC
Cuts at: 2329 2329
Size: 5425
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4873
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2783 4328 1132
Size: 97 1554 1545 2229
Fragments arranged by size:
2229 1554 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2567 1348
Size: 1219 4206
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2495 2527 3494 3526 5007 5039 2495
Size: 32 967 32 1481 32 2881
Fragments arranged by size:
2881 1481 967 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2574 2637 2574
Size: 63 5362
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2445 2445
Size: 5425
PspXI vC'TCGA_Gb
Cuts at: 2279 2279
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2263 2388 2394 2777 830
Size: 1433 125 6 383 3478
Fragments arranged by size:
3478 1433 383 125 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
PvuII CAG'CTG
Cuts at: 2389 2452 2389
Size: 63 5362
SacII CC_GC'GG
Cuts at: 2232 2303 2232
Size: 71 5354
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SanDI GG'GwC_CC
Cuts at: 2637 2637
Size: 5425
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2259 2384 2390 2773 3908 4589 4780
Size: 219 1214 125 6 383 1135 681 191
Cuts at: 4780 826
Size: 1471
Fragments arranged by size:
1471 1214 1135 681 383 219 191 125
6
SfoI GGC'GCC
Cuts at: 2330 2330
Size: 5425
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 3529 4430 4692 820
Size: 197 926 336 1250 901 262 1553
Fragments arranged by size:
1553 1250 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2628 2864 513
Size: 552 1563 236 3074
Fragments arranged by size:
3074 1563 552 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 3452 3469 3622 3807 4252 325
Size: 1255 909 963 17 153 185 445 1498
Fragments arranged by size:
1498 1255 963 909 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 2279 1943
Size: 336 5089
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PsrI RsrII SacI SapI SexAI SfiI SrfI
SwaI XcmI