Restriction map for
pFN21AB7504
(Circular) (Six-base) MAPSORT of: pFN21AB7504.seq Check: 4975 from: 1 to: 5419
pFN21AB7504 5419 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3229 278
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 1937
Size: 825 4594
Acc65I G'GTAC_C
Cuts at: 2740 2740
Size: 5419
AclI AA'CG_TT
Cuts at: 1563 3546 3919 1563
Size: 1983 373 3063
Fragments arranged by size:
3063 1983 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3482 4866 5134 1632
Size: 1850 1384 268 1917
Fragments arranged by size:
1917 1850 1384 268
AfeI AGC'GCT
Cuts at: 2366 2366
Size: 5419
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5222
AflIII A'CryG_T
Cuts at: 1203 2043 4318 5295 1203
Size: 840 2275 977 1327
Fragments arranged by size:
2275 1327 977 840
AgeI A'CCGG_T
Cuts at: 4250 4250
Size: 5419
AhdI GACnn_n'nnGTC
Cuts at: 4148 4148
Size: 5419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2448 1184
Size: 1264 4155
AlwNI CAG_nnn'CTG
Cuts at: 2032 2161 2395 2450 3162 4734 2032
Size: 129 234 55 712 1572 2717
Fragments arranged by size:
2717 1572 712 234 129 55
ApaI G_GGCC'C
Cuts at: 2597 2603 2673 2597
Size: 6 70 5343
Fragments arranged by size:
5343 70 6
ApaLI G'TGCA_C
Cuts at: 3476 4632 3476
Size: 1156 4263
ApoI r'AATT_y
Cuts at: 1493 2728 2954 3106 5215 1493
Size: 1235 226 152 2109 1697
Fragments arranged by size:
2109 1697 1235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3973 160
Size: 872 2941 1606
Fragments arranged by size:
2941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2596 2666 2744 1870
Size: 73 152 501 70 78 4545
Fragments arranged by size:
4545 501 152 78 73 70
BamHI G'GATC_C
Cuts at: 2749 3116 2749
Size: 367 5052
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2740 4195 618
Size: 325 217 627 231 722 1455 1842
Fragments arranged by size:
1842 1455 722 627 325 231 217
BanII G_rGCy'C
Cuts at: 1391 2117 2597 2603 2673 2738 1391
Size: 726 480 6 70 65 4072
Fragments arranged by size:
4072 726 480 70 65 6
BbeI G_GCGC'C
Cuts at: 2022 2022
Size: 5419
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3610 3644 1903
Size: 34 1673 34 3678
Fragments arranged by size:
3678 1673 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3311 4521 3311
Size: 1210 4209
BclI T'GATC_A
Cuts at: 1548 2037 1548
Size: 489 4930
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2462 2555 4030
Size: 107 122 71 631 605 790 93 1475
Cuts at: 4030 136
Size: 1525
Fragments arranged by size:
1525 1475 790 631 605 122 107 93
71
BglII A'GATC_T
Cuts at: 5414 5414
Size: 5419
BlpI GC'TnA_GC
Cuts at: 2111 2306 2836 2111
Size: 195 530 4694
Fragments arranged by size:
4694 530 195
Bme1580I G_kGCm'C
Cuts at: 1790 2597 2603 2673 3480 4636 1790
Size: 807 6 70 807 1156 2573
Fragments arranged by size:
2573 1156 807 807 70 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2637 3150 4108
Size: 416 53 83 399 24 1220 513 958
Cuts at: 4108 442
Size: 1753
Fragments arranged by size:
1753 1220 958 513 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5419
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2090 2090
Size: 5419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 4079 1239
Size: 947 1893 2579
Fragments arranged by size:
2579 1893 947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3544 4409 4707 3544
Size: 865 298 4256
Fragments arranged by size:
4256 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 4082 882
Size: 617 579 2004 2219
Fragments arranged by size:
2219 2004 617 579
BsaAI yAC'GTr
Cuts at: 493 1204 3210 493
Size: 711 2006 2702
Fragments arranged by size:
2702 2006 711
BsaBI GATnn'nnATC
Cuts at: 5413 5413
Size: 5419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 3226 3608 4243
Size: 53 83 186 936 486 1207 382 635
Cuts at: 4243 275
Size: 1451
Fragments arranged by size:
1451 1207 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3852 4250 4524 4671 5082 3852
Size: 398 274 147 411 4189
Fragments arranged by size:
4189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5194
Fragments arranged by size:
5194 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5419
BseYI C'CCAG_C
Cuts at: 2025 2100 2648 4622 5118 2025
Size: 75 548 1974 496 2326
Fragments arranged by size:
2326 1974 548 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4846
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2466 2559 2869 3630 4658 1540
Size: 275 186 465 93 310 761 1028 2301
Fragments arranged by size:
2301 1028 761 465 310 275 186 93
BsiHKAI G_wGCw'C
Cuts at: 3480 3565 4636 3480
Size: 85 1071 4263
Fragments arranged by size:
4263 1071 85
BsmI GAATG_Cn'
Cuts at: 2930 3023 2930
Size: 93 5326
BsmBI CGTCTCn'nnnn_
Cuts at: 2061 2061
Size: 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2597 2603 2673 2738 3480 3565
Size: 399 327 480 6 70 65 742 85
Cuts at: 3565 4636 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 327 85 70
65 6
BspEI T'CCGG_A
Cuts at: 5082 5082
Size: 5419
BspHI T'CATG_A
Cuts at: 2343 3308 5038 2343
Size: 965 1730 2724
Fragments arranged by size:
2724 1730 965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2774 844
Size: 1214 716 3489
Fragments arranged by size:
3489 1214 716
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 3306 1394
Size: 429 193 1290 3507
Fragments arranged by size:
3507 1290 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 3914 4088 65
Size: 1183 2666 174 1396
Fragments arranged by size:
2666 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 2498 2573 4063 4250 1700
Size: 110 382 306 75 1490 187 2869
Fragments arranged by size:
2869 1490 382 306 187 110 75
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5419
BssHII G'CGCG_C
Cuts at: 1925 2478 1925
Size: 553 4866
BssSI C'ACGA_G
Cuts at: 3479 4491 3479
Size: 1012 4407
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 2450 794
Size: 1367 289 3763
Fragments arranged by size:
3763 1367 289
BstBI TT'CG_AA
Cuts at: 4223 4223
Size: 5419
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5419
BstYI r'GATC_y
Cuts at: 1972 2056 2142 2749 3116 3501 3518 4970 5056
Size: 84 86 607 367 385 17 1452 86
Cuts at: 5056 5068 5414 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 607 385 367 346 86 86
84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 2460 2682 513
Size: 552 1053 342 222 3250
Fragments arranged by size:
3250 1053 552 342 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2185 487
Size: 683 56 959 3721
Fragments arranged by size:
3721 959 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3729 3749 2935
Size: 794 20 4605
Fragments arranged by size:
4605 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5384
DraI TTT'AAA
Cuts at: 2723 2723
Size: 5419
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4426 809
Size: 507 3110 1802
Fragments arranged by size:
3110 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 2547 2556
Size: 54 1425 50 275 18 633 84 9
Cuts at: 2556 2588 2685 2866 3755 5384 8
Size: 32 97 181 889 1629 43
Fragments arranged by size:
1629 1425 889 633 275 181 97 84
54 50 43 32 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2463 2556 2866 1537
Size: 275 651 93 310 4090
Fragments arranged by size:
4090 651 310 275 93
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 3349 1353
Size: 728 1268 3423
Fragments arranged by size:
3423 1268 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4006 4380 4526 387
Size: 1064 138 2417 374 146 1280
Fragments arranged by size:
2417 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 3482 4079 4866 5134 1239
Size: 393 554 1296 597 787 268 1524
Fragments arranged by size:
1524 1296 787 597 554 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2399
Size: 5419
EcoO109I rG'GnC_Cy
Cuts at: 2392 2600 2670 2392
Size: 208 70 5141
Fragments arranged by size:
5141 208 70
EcoRI G'AATT_C
Cuts at: 2728 3106 2728
Size: 378 5041
FspI TGC'GCA
Cuts at: 3925 3925
Size: 5419
HaeII r_GCGC'y
Cuts at: 1678 2022 2368 4566 1678
Size: 344 346 2198 2531
Fragments arranged by size:
2531 2198 346 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4060 4092 4134 4166 1373
Size: 32 135 32 2488 32 42 32 2626
Fragments arranged by size:
2626 2488 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2763 3009 669
Size: 988 281 825 246 3079
Fragments arranged by size:
3079 988 825 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5419
HpaI GTT'AAC
Cuts at: 3009 3009
Size: 5419
KasI G'GCGC_C
Cuts at: 2018 2018
Size: 5419
KpnI G_GTAC'C
Cuts at: 2744 2744
Size: 5419
MfeI C'AATT_G
Cuts at: 3018 3018
Size: 5419
MluI A'CGCG_T
Cuts at: 5295 5295
Size: 5419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 4533 4717 1305
Size: 739 2489 184 2007
Fragments arranged by size:
2489 2007 739 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3597
Fragments arranged by size:
3597 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2430 3377 3736 3895 518
Size: 648 1264 947 359 159 2042
Fragments arranged by size:
2042 1264 947 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2120 2136 2149 2447 2462 2512 2615
Size: 504 10 16 13 298 15 50 103
Cuts at: 2615 2835 3512 4660 4905 1606
Size: 220 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 504 298 245 220 103
50 16 15 13 10
NaeI GCC'GGC
Cuts at: 2194 2500 2575 2194
Size: 306 75 5038
Fragments arranged by size:
5038 306 75
NarI GG'CG_CC
Cuts at: 2019 2019
Size: 5419
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4867
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5419
NgoMIV G'CCGG_C
Cuts at: 2192 2498 2573 2192
Size: 306 75 5038
Fragments arranged by size:
5038 306 75
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5419
NotI GC'GGCC_GC
Cuts at: 2463 2866 2463
Size: 403 5016
NspI r_CATG'y
Cuts at: 1132 1229 2047 2777 4322 1132
Size: 97 818 730 1545 2229
Fragments arranged by size:
2229 1545 818 730 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5419
PciI A'CATG_T
Cuts at: 2043 4318 2043
Size: 2275 3144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 4132
PmeI GTTT'AAAC
Cuts at: 2723 2723
Size: 5419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 5001 5033 3488
Size: 32 1481 32 3874
Fragments arranged by size:
3874 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5419
PsiI TTA'TAA
Cuts at: 2989 2989
Size: 5419
PspOMI G'GGCC_C
Cuts at: 2593 2599 2669 2593
Size: 6 70 5343
Fragments arranged by size:
5343 70 6
PstI C_TGCA'G
Cuts at: 830 2771 830
Size: 1941 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5419
PvuII CAG'CTG
Cuts at: 2149 2447 2149
Size: 298 5121
SacII CC_GC'GG
Cuts at: 2121 2463 2121
Size: 342 5077
SalI G'TCGA_C
Cuts at: 1936 2761 1936
Size: 825 4594
SbfI CC_TGCA'GG
Cuts at: 2771 2771
Size: 5419
ScaI AGT'ACT
Cuts at: 3667 5379 3667
Size: 1712 3707
SfcI C'TryA_G
Cuts at: 826 1045 2767 3902 4583 4774 826
Size: 219 1722 1135 681 191 1471
Fragments arranged by size:
1722 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2555 2555
Size: 5419
SfoI GGC'GCC
Cuts at: 2020 2020
Size: 5419
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5419
SmaI CCC'GGG
Cuts at: 1872 2598 2668 2746 1872
Size: 726 70 78 4545
Fragments arranged by size:
4545 726 78 70
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3523 4424 4686 820
Size: 197 926 1580 901 262 1553
Fragments arranged by size:
1580 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3210 493
Size: 2717 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5419
SphI G_CATG'C
Cuts at: 1132 2777 1132
Size: 1645 3774
SrfI GCCC'GGGC
Cuts at: 2598 2668 2598
Size: 70 5349
SspI AAT'ATT
Cuts at: 5 52 3343 5
Size: 47 3291 2081
Fragments arranged by size:
3291 2081 47
StuI AGG'CCT
Cuts at: 1517 2405 1517
Size: 888 4531
StyI C'CwwG_G
Cuts at: 513 1065 2858 513
Size: 552 1793 3074
Fragments arranged by size:
3074 1793 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3446 3463 3616 3801 4246 325
Size: 1255 1866 17 153 185 445 1498
Fragments arranged by size:
1866 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3665 5377 96
Size: 274 80 33 51 1443 1688 1712 138
Fragments arranged by size:
1712 1688 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5379 5411 5379
Size: 32 5387
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5419
XbaI T'CTAG_A
Cuts at: 2755 2755
Size: 5419
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5419
XmaI C'CCGG_G
Cuts at: 1870 2596 2666 2744 1870
Size: 726 70 78 4545
Fragments arranged by size:
4545 726 78 70
XmnI GAAnn'nnTTC
Cuts at: 3548 3548
Size: 5419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3227 276
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SwaI
XcmI