Restriction map for
pFN21AB7443
(Circular) (Six-base) MAPSORT of: pFN21AB7443.seq Check: 2695 from: 1 to: 5581
pFN21AB7443 5581 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2274 2382 2855 2274
Size: 108 473 5000
Fragments arranged by size:
5000 473 108
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2667 3391 278
Size: 53 83 186 936 1131 724 2468
Fragments arranged by size:
2468 1131 936 724 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 3644 5028 5296 1632
Size: 710 1302 1384 268 1917
Fragments arranged by size:
1917 1384 1302 710 268
AflII C'TTAA_G
Cuts at: 820 1017 2035 820
Size: 197 1018 4366
Fragments arranged by size:
4366 1018 197
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 2153 2681 1166
Size: 987 528 4066
Fragments arranged by size:
4066 987 528
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5581
AlwNI CAG_nnn'CTG
Cuts at: 2638 3324 4896 2638
Size: 686 1572 3323
Fragments arranged by size:
3323 1572 686
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2890 3116 3268 5377 1493
Size: 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 2833 2906 1870
Size: 73 684 206 73 4545
Fragments arranged by size:
4545 684 206 73 73
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2370 2396 2902 4357 618
Size: 325 217 627 583 26 506 1455 1842
Fragments arranged by size:
1842 1455 627 583 506 325 217 26
BanII G_rGCy'C
Cuts at: 1391 2023 2223 2900 1391
Size: 632 200 677 4072
Fragments arranged by size:
4072 677 632 200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2369 2403 3772 3806 1903
Size: 34 432 34 1369 34 3678
Fragments arranged by size:
3678 1369 432 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4192 136
Size: 107 122 71 631 605 2520 1525
Fragments arranged by size:
2520 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5576 5576
Size: 5581
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 3642 4798 1790
Size: 1852 1156 2573
Fragments arranged by size:
2573 1852 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2718 3312 4270
Size: 416 53 83 399 24 1301 594 958
Cuts at: 4270 442
Size: 1753
Fragments arranged by size:
1753 1301 958 594 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 4241 1239
Size: 921 2081 2579
Fragments arranged by size:
2579 2081 921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3706 4571 4869 3706
Size: 865 298 4418
Fragments arranged by size:
4418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4244 882
Size: 617 2745 2219
Fragments arranged by size:
2745 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2664 3388 3770 4405
Size: 53 83 186 936 1131 724 382 635
Cuts at: 4405 275
Size: 1451
Fragments arranged by size:
1451 1131 936 724 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4014 4412 4686 4833 5244 4014
Size: 398 274 147 411 4351
Fragments arranged by size:
4351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5356
Fragments arranged by size:
5356 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5581
BseYI C'CCAG_C
Cuts at: 2303 4784 5280 2303
Size: 2481 496 2604
Fragments arranged by size:
2604 2481 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 2807 1163
Size: 573 831 240 3937
Fragments arranged by size:
3937 831 573 240
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2542 3031 3792 4820 1540
Size: 275 186 541 489 761 1028 2301
Fragments arranged by size:
2301 1028 761 541 489 275 186
BsiHKAI G_wGCw'C
Cuts at: 3642 3727 4798 3642
Size: 85 1071 4425
Fragments arranged by size:
4425 1071 85
BsiWI C'GTAC_G
Cuts at: 2159 2159
Size: 5581
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
BsmBI CGTCTCn'nnnn_
Cuts at: 2356 2356
Size: 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2223 2900 3642 3727 4798 1391
Size: 399 233 200 677 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 677 399 233 200 85
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2382 2855 2936 844
Size: 1430 108 473 81 3489
Fragments arranged by size:
3489 1430 473 108 81
BsrBI CCG'CTC
Cuts at: 1394 1823 2244 2614 3468 1394
Size: 429 421 370 854 3507
Fragments arranged by size:
3507 854 429 421 370
BsrDI GCAATG_nn'
Cuts at: 65 1248 4076 4250 65
Size: 1183 2828 174 1396
Fragments arranged by size:
2828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2119 2460 4225 4412 1700
Size: 110 309 341 1765 187 2869
Fragments arranged by size:
2869 1765 341 309 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2911 3278 3663 3680 5132 5218 5230 5576
Size: 939 367 385 17 1452 86 12 346
Cuts at: 5576 1972
Size: 1977
Fragments arranged by size:
1977 1452 939 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2186 2392 2571 2877 513
Size: 552 1121 206 179 306 3217
Fragments arranged by size:
3217 1121 552 306 206 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3891 3911 3097
Size: 794 20 4767
Fragments arranged by size:
4767 794 20
ClaI AT'CG_AT
Cuts at: 1554 2094 1554
Size: 540 5041
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DraIII CAC_nnn'GTG
Cuts at: 2069 2069
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 3028 3917
Size: 54 1425 50 275 18 333 865 889
Cuts at: 3917 5546 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 865 333 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 3511 1353
Size: 965 1193 3423
Fragments arranged by size:
3423 1193 965
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2342 3644 4241 5028 5296 1239
Size: 393 528 182 1302 597 787 268 1524
Fragments arranged by size:
1524 1302 787 597 528 393 268 182
EcoO109I rG'GnC_Cy
Cuts at: 2491 2509 2593 2491
Size: 18 84 5479
Fragments arranged by size:
5479 84 18
EcoRI G'AATT_C
Cuts at: 2890 3268 2890
Size: 378 5203
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 2581 4728 1678
Size: 903 2147 2531
Fragments arranged by size:
2531 2147 903
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2701 2733 4222
Size: 32 135 32 495 32 602 32 1489
Cuts at: 4222 4254 4296 4328 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1489 602 495 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2925 3171 669
Size: 988 281 987 246 3079
Fragments arranged by size:
3079 988 987 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 4695 4879 1305
Size: 1272 2118 184 2007
Fragments arranged by size:
2118 2007 1272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2420 2681 2724 3539 3898 4057
Size: 648 987 267 261 43 815 359 159
Cuts at: 4057 518
Size: 2042
Fragments arranged by size:
2042 987 815 648 359 267 261 159
43
MspA1I CmG'CkG
Cuts at: 1606 2394 2563 2599 2821 2997 3674 4822 5067
Size: 788 169 36 222 176 677 1148 245
Cuts at: 5067 1606
Size: 2120
Fragments arranged by size:
2120 1148 788 677 245 222 176 169
36
NaeI GCC'GGC
Cuts at: 2462 2462
Size: 5581
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5029
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NgoMIV G'CCGG_C
Cuts at: 2460 2460
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2533 2939 4484 1132
Size: 97 1304 406 1545 2229
Fragments arranged by size:
2229 1545 1304 406 97
PasI CC'CwG_GG
Cuts at: 1348 2752 1348
Size: 1404 4177
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 4432
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2491 2593 2491
Size: 102 5479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2290 2933 830
Size: 1460 643 3478
Fragments arranged by size:
3478 1460 643
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
PvuII CAG'CTG
Cuts at: 2563 2821 2563
Size: 258 5323
SacII CC_GC'GG
Cuts at: 2395 2395
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SfcI C'TryA_G
Cuts at: 826 1045 2286 2929 4064 4745 4936 826
Size: 219 1241 643 1135 681 191 1471
Fragments arranged by size:
1471 1241 1135 681 643 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2835 2908 1872
Size: 963 73 4545
Fragments arranged by size:
4545 963 73
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2035 3685 4586 4848 820
Size: 197 926 92 1650 901 262 1553
Fragments arranged by size:
1650 1553 926 901 262 197 92
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2533 2939 1132
Size: 1401 406 3774
Fragments arranged by size:
3774 1401 406
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2444 3020 513
Size: 552 1379 576 3074
Fragments arranged by size:
3074 1379 576 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2882 3608 3625 3778 3963 4408 325
Size: 1255 1302 726 17 153 185 445 1498
Fragments arranged by size:
1498 1302 1255 726 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3827 5539 96
Size: 274 80 33 51 1443 1850 1712 138
Fragments arranged by size:
1850 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5541 5573 5541
Size: 32 5549
Tth111I GACn'n_nGTC
Cuts at: 1316 2489 1316
Size: 1173 4408
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2683 2683
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2833 2906 1870
Size: 963 73 4545
Fragments arranged by size:
4545 963 73
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2665 3389 276
Size: 53 83 186 936 1131 724 2468
Fragments arranged by size:
2468 1131 936 724 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI