Restriction map for
pFN21AB7431
(Circular) (Six-base) MAPSORT of: pFN21AB7431.seq Check: 1588 from: 1 to: 5629
pFN21AB7431 5629 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2906 3439 278
Size: 53 83 186 936 1370 533 2468
Fragments arranged by size:
2468 1370 936 533 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 1937
Size: 1035 4594
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2107 2907 3692 5076 5344 1632
Size: 475 800 785 1384 268 1917
Fragments arranged by size:
1917 1384 800 785 475 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2873 1184
Size: 1689 3940
AlwNI CAG_nnn'CTG
Cuts at: 2324 3372 4944 2324
Size: 1048 1572 3009
Fragments arranged by size:
3009 1572 1048
ApaI G_GGCC'C
Cuts at: 2310 2310
Size: 5629
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 1870
Size: 73 1011 4545
Fragments arranged by size:
4545 1011 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2114 2450 2950 4405
Size: 325 217 627 248 79 336 500 1455
Cuts at: 4405 618
Size: 1842
Fragments arranged by size:
1842 1455 627 500 336 325 248 217
79
BanII G_rGCy'C
Cuts at: 1391 2021 2310 2948 1391
Size: 630 289 638 4072
Fragments arranged by size:
4072 638 630 289
BbeI G_GCGC'C
Cuts at: 2454 2454
Size: 5629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 928
Size: 971 645 4013
Fragments arranged by size:
4013 971 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3820 3854 1903
Size: 34 50 34 1799 34 3678
Fragments arranged by size:
3678 1799 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2727 3521 4731 2727
Size: 794 1210 3625
Fragments arranged by size:
3625 1210 794
BclI T'GATC_A
Cuts at: 1548 2520 1548
Size: 972 4657
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2016 2044 2279
Size: 107 122 71 631 605 344 28 235
Cuts at: 2279 2387 4240 136
Size: 108 1853 1525
Fragments arranged by size:
1853 1525 631 605 344 235 122 108
107 71 28
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 2546 3046 2546
Size: 500 5129
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2284 2310 3690 4846 1790
Size: 327 167 26 1380 1156 2573
Fragments arranged by size:
2573 1380 1156 327 167 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2911 3360 4318
Size: 416 53 83 399 24 1494 449 958
Cuts at: 4318 442
Size: 1753
Fragments arranged by size:
1753 1494 958 449 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2381 2585 4289 1239
Size: 1112 30 204 1704 2579
Fragments arranged by size:
2579 1704 1112 204 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2451 2903 3436 3818
Size: 53 83 186 936 918 452 533 382
Cuts at: 3818 4453 275
Size: 635 1451
Fragments arranged by size:
1451 936 918 635 533 452 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2719 4062 4460 4734 4881 5292 2719
Size: 1343 398 274 147 411 3056
Fragments arranged by size:
3056 1343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2255 2258 2282 2348 1186
Size: 1015 54 3 24 66 4467
Fragments arranged by size:
4467 1015 66 54 24 3
BseYI C'CCAG_C
Cuts at: 2274 4832 5328 2274
Size: 2558 496 2575
Fragments arranged by size:
2575 2558 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2310 1163
Size: 573 423 151 4482
Fragments arranged by size:
4482 573 423 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2154 3079 3840 4868 1540
Size: 275 186 153 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 3690 3775 4846 3690
Size: 85 1071 4473
Fragments arranged by size:
4473 1071 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
BsmBI CGTCTCn'nnnn_
Cuts at: 2894 2894
Size: 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2117 2284 2310 2948 3690 3775
Size: 399 231 96 167 26 638 742 85
Cuts at: 3775 4846 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 638 399 231 167 96
85 26
BspEI T'CCGG_A
Cuts at: 2719 5292 2719
Size: 2573 3056
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 844
Size: 2140 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2260 2419 3516 1394
Size: 429 437 159 1097 3507
Fragments arranged by size:
3507 1097 437 429 159
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2365 4273 4460 1700
Size: 110 555 1908 187 2869
Fragments arranged by size:
2869 1908 555 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 2252 3689 4701 2252
Size: 1437 1012 3180
Fragments arranged by size:
3180 1437 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2293 794
Size: 1499 4130
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2712 2712
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2807 2826 2959 3326 3711 3728 5180 5266
Size: 835 19 133 367 385 17 1452 86
Cuts at: 5266 5278 5624 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 835 385 367 346 133 86
19 17 12
Bsu36I CC'TnA_GG
Cuts at: 2013 2013
Size: 5629
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5077
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 3145 3939 3959 3145
Size: 794 20 4815
Fragments arranged by size:
4815 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2689 2724 529
Size: 35 2125 35 3434
Fragments arranged by size:
3434 2125 35 35
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2320 4636 809
Size: 507 1004 2316 1802
Fragments arranged by size:
2316 1802 1004 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2343 3076
Size: 54 1425 50 275 18 321 192 733
Cuts at: 3076 3965 5594 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 733 321 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2151 3076 1537
Size: 275 339 925 4090
Fragments arranged by size:
4090 925 339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2571 2883 3559 1353
Size: 1218 312 676 3423
Fragments arranged by size:
3423 1218 676 312
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2234 2252 4216 4590 4736 387
Size: 1064 138 645 18 1964 374 146 1280
Fragments arranged by size:
1964 1280 1064 645 374 146 138 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2351 2381 2585 2907 3692 4289
Size: 393 475 244 30 204 322 785 597
Cuts at: 4289 5076 5344 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 785 597 475 393 322 268
244 204 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2834 2834
Size: 5629
EcoO109I rG'GnC_Cy
Cuts at: 2248 2839 2248
Size: 591 5038
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 2454 4776 1678
Size: 776 2322 2531
Fragments arranged by size:
2531 2322 776
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2439 2471 4270 4302 4344
Size: 32 135 32 867 32 1799 32 42
Cuts at: 4344 4376 1373
Size: 32 2626
Fragments arranged by size:
2626 1799 867 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KasI G'GCGC_C
Cuts at: 2450 2450
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 2706 3228 2706
Size: 522 5107
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 4743 4927 1305
Size: 879 2559 184 2007
Fragments arranged by size:
2559 2007 879 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3807
Fragments arranged by size:
3807 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3587 3946 4105 518
Size: 648 2421 359 159 2042
Fragments arranged by size:
2421 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2278 2404 3045 3722 4870 5115 1606
Size: 672 126 641 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 641 245 126
NarI GG'CG_CC
Cuts at: 2451 2451
Size: 5629
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5077
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2545 2987 4532 1132
Size: 97 1316 442 1545 2229
Fragments arranged by size:
2229 1545 1316 442 97
PasI CC'CwG_GG
Cuts at: 1348 2303 1348
Size: 955 4674
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5629
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2839 2839
Size: 5629
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5629
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2306 2306
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2981 830
Size: 2151 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2977 4112 4793 4984 826
Size: 219 1932 1135 681 191 1471
Fragments arranged by size:
1932 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2452 2452
Size: 5629
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 2087 2390 2738 1517
Size: 570 303 348 4408
Fragments arranged by size:
4408 570 348 303
StyI C'CwwG_G
Cuts at: 513 1065 2088 2106 2655 3068 513
Size: 552 1023 18 549 413 3074
Fragments arranged by size:
3074 1023 552 549 413 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3656 3673 3826 4011 4456 325
Size: 1255 2076 17 153 185 445 1498
Fragments arranged by size:
2076 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2662 2468
Size: 194 5435
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2904 3437 276
Size: 53 83 186 936 1370 533 2468
Fragments arranged by size:
2468 1370 936 533 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI