Restriction map for
pFN21AB7430
(Circular) (Six-base) MAPSORT of: pFN21AB7430.seq Check: 581 from: 1 to: 6193
pFN21AB7430 6193 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3174 3174
Size: 6193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 3536 1937
Size: 823 776 4594
Fragments arranged by size:
4594 823 776
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4256 5640 5908 1632
Size: 2624 1384 268 1917
Fragments arranged by size:
2624 1917 1384 268
AfeI AGC'GCT
Cuts at: 2329 2329
Size: 6193
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 5092 6069 1203
Size: 3889 977 1327
Fragments arranged by size:
3889 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3363 1184
Size: 2179 4014
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2696 3332 3502 3728 3880 5989 1493
Size: 1203 636 170 226 152 2109 1697
Fragments arranged by size:
2109 1697 1203 636 226 170 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 3518 1870
Size: 73 1575 4545
Fragments arranged by size:
4545 1575 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3514 4969 618
Size: 325 217 627 1727 1455 1842
Fragments arranged by size:
1842 1727 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 3202 3512 1391
Size: 671 1140 310 4072
Fragments arranged by size:
4072 1140 671 310
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2997 928
Size: 971 1098 4124
Fragments arranged by size:
4124 1098 971
BbvCI CC'TCA_GC
Cuts at: 3485 3485
Size: 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2517 4804 136
Size: 107 122 71 631 605 845 2287 1525
Fragments arranged by size:
2287 1525 845 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6188 6188
Size: 6193
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 4254 5410 1790
Size: 2464 1156 2573
Fragments arranged by size:
2573 2464 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3924 4882 442
Size: 416 53 83 399 24 2507 958 1753
Fragments arranged by size:
2507 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3269 4853 1239
Size: 2030 1584 2579
Fragments arranged by size:
2579 2030 1584
Bpu10I CC'TnA_GC
Cuts at: 2571 2636 3485 2571
Size: 65 849 5279
Fragments arranged by size:
5279 849 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4856 882
Size: 617 3357 2219
Fragments arranged by size:
3357 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4000 4382 5017 275
Size: 53 83 186 936 2467 382 635 1451
Fragments arranged by size:
2467 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2026 4626 5024 5298 5445 5856 2026
Size: 2600 398 274 147 411 2363
Fragments arranged by size:
2600 2363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2191 1178
Size: 30 165 30 758 30 5180
Fragments arranged by size:
5180 758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6193
BseYI C'CCAG_C
Cuts at: 5396 5892 5396
Size: 496 5697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5620
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2041 3142 3643 4404 5432 1540
Size: 275 186 40 1101 501 761 1028 2301
Fragments arranged by size:
2301 1101 1028 761 501 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 4254 4339 5410 4254
Size: 85 1071 5037
Fragments arranged by size:
5037 1071 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 3202 3512 4254 4339 5410 1391
Size: 399 272 1140 310 742 85 1071 2174
Fragments arranged by size:
2174 1140 1071 742 399 310 272 85
BspEI T'CCGG_A
Cuts at: 2026 5856 2026
Size: 3830 2363
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2113 3013 3174 3548 844
Size: 1269 900 161 374 3489
Fragments arranged by size:
3489 1269 900 374 161
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 4837 5024 1700
Size: 110 265 2762 187 2869
Fragments arranged by size:
2869 2762 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2526 794
Size: 1732 4461
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 3523 3890 4275 4292 5744 5830 5842 6188
Size: 1551 367 385 17 1452 86 12 346
Cuts at: 6188 1972
Size: 1977
Fragments arranged by size:
1977 1551 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2490 3709 4503 4523 2490
Size: 1219 794 20 4160
Fragments arranged by size:
4160 1219 794 20
ClaI AT'CG_AT
Cuts at: 1554 3138 1554
Size: 1584 4609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2456 2509
Size: 54 1425 50 275 18 208 418 53
Cuts at: 2509 3640 4529 6158 8
Size: 1131 889 1629 43
Fragments arranged by size:
1629 1425 1131 889 418 275 208 54
53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2038 3640 1537
Size: 275 226 1602 4090
Fragments arranged by size:
4090 1602 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 2298 2437 2529 2737 3411 4123 1353
Size: 945 139 92 208 674 712 3423
Fragments arranged by size:
3423 945 712 674 208 139 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2070 4780 5154 5300 387
Size: 1064 138 481 2710 374 146 1280
Fragments arranged by size:
2710 1280 1064 481 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3269 4256 4853 5640 5908 1239
Size: 393 1637 987 597 787 268 1524
Fragments arranged by size:
1637 1524 987 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2931 2931
Size: 6193
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 3128 2737
Size: 391 5802
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 2017 2331 5340 1678
Size: 339 314 3009 2531
Fragments arranged by size:
3009 2531 339 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 4834 4866 4908
Size: 32 135 32 503 32 2727 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2727 2626 503 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2849 3473 3537 3783 669
Size: 988 281 911 624 64 246 3079
Fragments arranged by size:
3079 988 911 624 281 246 64
HindIII A'AGCT_T
Cuts at: 748 3344 748
Size: 2596 3597
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 2598 3792 2598
Size: 1194 4999
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5491 1305
Size: 4002 184 2007
Fragments arranged by size:
4002 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2511 10
Size: 54 1425 343 679 3692
Fragments arranged by size:
3692 1425 679 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2095 3168 4151 4510 4669 518
Size: 648 929 1073 983 359 159 2042
Fragments arranged by size:
2042 1073 983 929 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2914 3609 4286 5434 5679 1606
Size: 431 877 695 677 1148 245 2120
Fragments arranged by size:
2120 1148 877 695 677 431 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 6193
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5641
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3209 3551 5096 1132
Size: 97 1980 342 1545 2229
Fragments arranged by size:
2229 1980 1545 342 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2850 2882 2850
Size: 32 6161
PstI C_TGCA'G
Cuts at: 830 3545 830
Size: 2715 3478
PvuI CG_AT'CG
Cuts at: 2001 3142 2001
Size: 1141 5052
PvuII CAG'CTG
Cuts at: 2914 2914
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2707 2757 3541 4676 5357 5548 826
Size: 219 1662 50 784 1135 681 191 1471
Fragments arranged by size:
1662 1471 1135 784 681 219 191 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4297 5198 5460 820
Size: 197 926 2354 901 262 1553
Fragments arranged by size:
2354 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 2834 4117 5
Size: 47 2782 1283 2081
Fragments arranged by size:
2782 2081 1283 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2969 3632 513
Size: 552 1904 663 3074
Fragments arranged by size:
3074 1904 663 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2381 4439 6151
Size: 274 80 33 51 1443 404 2058 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
2058 1712 1443 404 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2122 2154 6153 6185 2122
Size: 32 3999 32 2130
Fragments arranged by size:
3999 2130 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2466 2466
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 2450 2994 3111 4322 2450
Size: 544 117 1211 4321
Fragments arranged by size:
4321 1211 544 117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI