Restriction map for
pFN21AB7428
(Circular) (Six-base) MAPSORT of: pFN21AB7428.seq Check: 6237 from: 1 to: 5941
pFN21AB7428 5941 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2422 2422
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3135 3284 1937
Size: 1198 149 4594
Fragments arranged by size:
4594 1198 149
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 4004 5388 5656 1632
Size: 561 1811 1384 268 1917
Fragments arranged by size:
1917 1811 1384 561 268
AfeI AGC'GCT
Cuts at: 2622 2622
Size: 5941
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 3684 5256 3684
Size: 1572 4369
ApaI G_GGCC'C
Cuts at: 2245 2252 3222 2245
Size: 7 970 4964
Fragments arranged by size:
4964 970 7
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2357 2880 3250 3476 3628 5737 1493
Size: 864 523 370 226 152 2109 1697
Fragments arranged by size:
2109 1697 864 523 370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2616 2730 3266 1870
Size: 73 673 114 536 4545
Fragments arranged by size:
4545 673 536 114 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2944 3262 4717 618
Size: 325 217 627 1157 318 1455 1842
Fragments arranged by size:
1842 1455 1157 627 325 318 217
BanII G_rGCy'C
Cuts at: 1391 2245 2252 2334 2643 2958 3222 3260 1391
Size: 854 7 82 309 315 264 38 4072
Fragments arranged by size:
4072 854 315 309 264 82 38 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BbvCI CC'TCA_GC
Cuts at: 2154 2223 2531 2154
Size: 69 308 5564
Fragments arranged by size:
5564 308 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2015 2049 2416 2450 4132 4166 1903
Size: 34 78 34 367 34 1682 34 3678
Fragments arranged by size:
3678 1682 367 78 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BfrBI ATG'CAT
Cuts at: 2834 2834
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2774 4552
Size: 107 122 71 631 605 337 765 1778
Cuts at: 4552 136
Size: 1525
Fragments arranged by size:
1778 1525 765 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 2624 2841 3358 2624
Size: 217 517 5207
Fragments arranged by size:
5207 517 217
Bme1580I G_kGCm'C
Cuts at: 1790 2245 2252 2418 3222 4002 5158 1790
Size: 455 7 166 804 780 1156 2573
Fragments arranged by size:
2573 1156 804 780 455 166 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2572 2776 2930
Size: 416 53 83 399 24 1155 204 154
Cuts at: 2930 3012 3672 4630 442
Size: 82 660 958 1753
Fragments arranged by size:
1753 1155 958 660 416 399 204 154
83 82 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4601 1239
Size: 1388 1974 2579
Fragments arranged by size:
2579 1974 1388
Bpu10I CC'TnA_GC
Cuts at: 2154 2223 2531 2739 2154
Size: 69 308 208 5356
Fragments arranged by size:
5356 308 208 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2399 3748 4130 4765
Size: 53 83 186 936 866 1349 382 635
Cuts at: 4765 275
Size: 1451
Fragments arranged by size:
1451 1349 936 866 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2109 4374 4772 5046 5193 5604 2109
Size: 2265 398 274 147 411 2446
Fragments arranged by size:
2446 2265 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2683 2713 1178
Size: 30 165 30 1280 30 4406
Fragments arranged by size:
4406 1280 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2225 2228 2240 2885 2966 1186
Size: 1012 27 3 12 645 81 4161
Fragments arranged by size:
4161 1012 645 81 27 12 3
BseYI C'CCAG_C
Cuts at: 2252 2746 2797 5144 5640 2252
Size: 494 51 2347 496 2553
Fragments arranged by size:
2553 2347 496 494 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3183 1163
Size: 573 1447 3921
Fragments arranged by size:
3921 1447 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2559 3391 4152 5180 1540
Size: 275 186 558 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 558 275 186
BsiHKAI G_wGCw'C
Cuts at: 2334 2439 2643 4002 4087 5158 2334
Size: 105 204 1359 85 1071 3117
Fragments arranged by size:
3117 1359 1071 204 105 85
BsiWI C'GTAC_G
Cuts at: 2601 2601
Size: 5941
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2245 2252 2334 2418 2439 2643 2958
Size: 399 455 7 82 84 21 204 315
Cuts at: 2958 3222 3260 4002 4087 5158 1391
Size: 264 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 455 399 315 264 204
85 84 82 38 21 7
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 3296 844
Size: 1578 874 3489
Fragments arranged by size:
3489 1578 874
BsrBI CCG'CTC
Cuts at: 1394 1823 2704 3058 3828 1394
Size: 429 881 354 770 3507
Fragments arranged by size:
3507 881 770 429 354
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 2291 4001 5013 2291
Size: 1710 1012 3219
Fragments arranged by size:
3219 1710 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 2464 1184
Size: 1280 4661
BstXI CCAn_nnnn'nTGG
Cuts at: 2276 2276
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936
Size: 1299 367 385 17 1452 86 12 346
Cuts at: 5936 1972
Size: 1977
Fragments arranged by size:
1977 1452 1299 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2127 3047 2127
Size: 920 5021
BtgI C'CryG_G
Cuts at: 513 1065 2004 3031 513
Size: 552 939 1027 3423
Fragments arranged by size:
3423 1027 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2900 3094 3457 4251 4271 2900
Size: 194 363 794 20 4570
Fragments arranged by size:
4570 794 363 194 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2556 3388 4277
Size: 54 1425 50 275 18 726 832 889
Cuts at: 4277 5906 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 832 726 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2556 3388 1537
Size: 275 744 832 4090
Fragments arranged by size:
4090 832 744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2711 2960 3048 3871 1353
Size: 801 557 249 88 823 3423
Fragments arranged by size:
3423 823 801 557 249 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2332 2641 2332
Size: 309 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2627 4004 4601 5388 5656 1239
Size: 393 561 434 1377 597 787 268 1524
Fragments arranged by size:
1524 1377 787 597 561 434 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2011 2122 2133 2241 2248 2249 2743 2756 2924
Size: 111 11 108 7 1 494 13 168
Cuts at: 2924 2984 3218 3219 2011
Size: 60 234 1 4733
Fragments arranged by size:
4733 494 234 168 111 108 60 13
11 7 1 1
EcoRI G'AATT_C
Cuts at: 2880 3250 3628 2880
Size: 370 378 5193
Fragments arranged by size:
5193 378 370
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2624 2739 5088 1678
Size: 946 115 2349 2531
Fragments arranged by size:
2531 2349 946 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373
Size: 32 135 32 3010 32 42 32 2626
Fragments arranged by size:
3010 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3212 5055 5239 1305
Size: 1907 1843 184 2007
Fragments arranged by size:
2007 1907 1843 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3899 4258 4417 518
Size: 648 2733 359 159 2042
Fragments arranged by size:
2733 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2222 2323 2845 3357 4034 5182 5427
Size: 471 145 101 522 512 677 1148 245
Cuts at: 5427 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 522 512 471 245 145
101
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NsiI A_TGCA'T
Cuts at: 2836 2836
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2073 2834 2838 3013 3299 4844 1132
Size: 97 844 761 4 175 286 1545 2229
Fragments arranged by size:
2229 1545 844 761 286 175 97 4
PasI CC'CwG_GG
Cuts at: 1348 2044 2245 2633 2803 3181 1348
Size: 696 201 388 170 378 4108
Fragments arranged by size:
4108 696 388 378 201 170
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 1108
Size: 942 4999
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2743 2756 2924 2743
Size: 13 168 5760
Fragments arranged by size:
5760 168 13
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2241 2248 3218 2241
Size: 7 970 4964
Fragments arranged by size:
4964 970 7
PspXI vC'TCGA_Gb
Cuts at: 2616 2616
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2019 3293 830
Size: 1189 1274 3478
Fragments arranged by size:
3478 1274 1189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2222 2323 2845 2222
Size: 101 522 5318
Fragments arranged by size:
5318 522 101
SacI G_AGCT'C
Cuts at: 2334 2643 2334
Size: 309 5632
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2756 2756
Size: 5941
SapI GCTCTTCn'nnn_
Cuts at: 2711 3048 2711
Size: 337 5604
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2015 2117 3289 4424 5105 5296 826
Size: 219 970 102 1172 1135 681 191 1471
Fragments arranged by size:
1471 1172 1135 970 681 219 191 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2616 4045 4946 5208 820
Size: 197 926 673 1429 901 262 1553
Fragments arranged by size:
1553 1429 926 901 673 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 2073 2834 2838 3013 3299 1132
Size: 941 761 4 175 286 3774
Fragments arranged by size:
3774 941 761 286 175 4
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2136 2472 2751 3380 513
Size: 552 939 132 336 279 629 3074
Fragments arranged by size:
3074 939 629 552 336 279 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2591 3968 3985 4138 4323 4768 325
Size: 1255 1011 1377 17 153 185 445 1498
Fragments arranged by size:
1498 1377 1255 1011 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 4187 5899
Size: 274 80 33 51 1443 603 1607 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
1712 1607 1443 603 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2683 2715 5901 5933 2683
Size: 32 3186 32 2691
Fragments arranged by size:
3186 2691 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2782 1316
Size: 1466 4475
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2479 2789 2479
Size: 310 5631
XhoI C'TCGA_G
Cuts at: 1943 2616 1943
Size: 673 5268
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BplI BsmBI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI