Restriction map for
pFN21AB7425
(Circular) (Six-base) MAPSORT of: pFN21AB7425.seq Check: 8103 from: 1 to: 6142
pFN21AB7425 6142 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 3322 3485 1937
Size: 673 712 163 4594
Fragments arranged by size:
4594 712 673 163
Acc65I G'GTAC_C
Cuts at: 2949 3463 2949
Size: 514 5628
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 2827 2990 3041 3206 3287 4205 5589
Size: 548 647 163 51 165 81 918 1384
Cuts at: 5589 5857 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 918 647 548 268 165 163
81 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 2646 3168 5041 6018 1203
Size: 1443 522 1873 977 1327
Fragments arranged by size:
1873 1443 1327 977 522
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 2053 2148 3885 5457 2053
Size: 95 1737 1572 2738
Fragments arranged by size:
2738 1737 1572 95
ApaLI G'TGCA_C
Cuts at: 2994 4199 5355 2994
Size: 1205 1156 3781
Fragments arranged by size:
3781 1205 1156
ApoI r'AATT_y
Cuts at: 1493 2335 2477 2584 2637 2777 3451 3677 3829
Size: 842 142 107 53 140 674 226 152
Cuts at: 3829 5938 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 842 674 226 152 142 140
107 53
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 3249 3467 1870
Size: 73 1306 218 4545
Fragments arranged by size:
4545 1306 218 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2941 2974 2941
Size: 33 6109
BamHI G'GATC_C
Cuts at: 3472 3839 3472
Size: 367 5775
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2871 2949 3463 4918 618
Size: 325 217 627 1084 78 514 1455 1842
Fragments arranged by size:
1842 1455 1084 627 514 325 217 78
BanII G_rGCy'C
Cuts at: 1391 2109 2885 3021 3031 3291 3345 3461 1391
Size: 718 776 136 10 260 54 116 4072
Fragments arranged by size:
4072 776 718 260 136 116 54 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 928
Size: 971 323 4848
Fragments arranged by size:
4848 971 323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2633 4034 5244 2633
Size: 1401 1210 3531
Fragments arranged by size:
3531 1401 1210
BclI T'GATC_A
Cuts at: 1548 2865 1548
Size: 1317 4825
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3207 4753 136
Size: 107 122 71 631 605 1535 1546 1525
Fragments arranged by size:
1546 1535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2232 2491 6137 2232
Size: 259 3646 2237
Fragments arranged by size:
3646 2237 259
BlpI GC'TnA_GC
Cuts at: 2744 3215 3309 3559 2744
Size: 471 94 250 5327
Fragments arranged by size:
5327 471 250 94
Bme1580I G_kGCm'C
Cuts at: 1790 2998 4203 5359 1790
Size: 1208 1205 1156 2573
Fragments arranged by size:
2573 1208 1205 1156
BmgBI CAC'GTC
Cuts at: 1318 3171 3390 1318
Size: 1853 219 4070
Fragments arranged by size:
4070 1853 219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3873 4831 442
Size: 416 53 83 399 24 2456 958 1753
Fragments arranged by size:
2456 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2249 2376 2795 2822 3428 4802 1239
Size: 815 195 127 419 27 606 1374 2579
Fragments arranged by size:
2579 1374 815 606 419 195 127 27
Bpu10I CC'TnA_GC
Cuts at: 2687 3122 2687
Size: 435 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2453 3035 4267 5132 5430 2453
Size: 582 1232 865 298 3165
Fragments arranged by size:
3165 1232 865 582 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 2490 6136 2490
Size: 3646 2496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2219 3949 4331 4966
Size: 53 83 186 936 686 1730 382 635
Cuts at: 4966 275
Size: 1451
Fragments arranged by size:
1730 1451 936 686 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3364 4575 4973 5247 5394 5805 3364
Size: 1211 398 274 147 411 3701
Fragments arranged by size:
3701 1211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2013 2043 1178
Size: 30 165 30 610 30 5277
Fragments arranged by size:
5277 610 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2783 3281 1186
Size: 1597 498 4047
Fragments arranged by size:
4047 1597 498
BseYI C'CCAG_C
Cuts at: 2056 5345 5841 2056
Size: 3289 496 2357
Fragments arranged by size:
3289 2357 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 1163
Size: 573 456 5113
Fragments arranged by size:
5113 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 2793 2998 3031 3291 4203 4288 5359 2109
Size: 684 205 33 260 912 85 1071 2892
Fragments arranged by size:
2892 1071 912 684 260 205 85 33
BsmI GAATG_Cn'
Cuts at: 2577 3653 3746 2577
Size: 1076 93 4973
Fragments arranged by size:
4973 1076 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2793 2885 2998 3021 3031 3291
Size: 399 319 684 92 113 23 10 260
Cuts at: 3291 3345 3461 4203 4288 5359 1391
Size: 54 116 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 399 319 260 116
113 92 85 54 23 10
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2748 3201 3497 844
Size: 1904 453 296 3489
Fragments arranged by size:
3489 1904 453 296
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3169 4637 4811 65
Size: 1183 1921 1468 174 1396
Fragments arranged by size:
1921 1468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4786 4973 1700
Size: 110 2976 187 2869
Fragments arranged by size:
2976 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 2148 794
Size: 1259 95 4788
Fragments arranged by size:
4788 1259 95
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2232 2491 3351 3472 3839 4224 4241 5693
Size: 260 259 860 121 367 385 17 1452
Cuts at: 5693 5779 5791 6137 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 860 385 367 346 260 259
121 86 17 12
BstZ17I GTA'TAC
Cuts at: 2611 3323 2611
Size: 712 5430
Bsu36I CC'TnA_GG
Cuts at: 2795 2876 2795
Size: 81 6061
BtgI C'CryG_G
Cuts at: 513 1065 3174 513
Size: 552 2109 3481
Fragments arranged by size:
3481 2109 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 2996 3658 4452 4472 2996
Size: 662 794 20 4666
Fragments arranged by size:
4666 794 662 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2350 5149 809
Size: 507 1034 2799 1802
Fragments arranged by size:
2799 1802 1034 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2654 3589
Size: 54 1425 50 275 18 243 581 935
Cuts at: 3589 4478 6107 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 935 889 581 275 243 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2521 2720 3060 3071 4072 1353
Size: 761 407 199 340 11 1001 3423
Fragments arranged by size:
3423 1001 761 407 340 199 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2311 4729 5103 5249 387
Size: 1064 138 722 2418 374 146 1280
Fragments arranged by size:
2418 1280 1064 722 374 146 138
EcoICRI GAG'CTC
Cuts at: 2107 3029 3289 2107
Size: 922 260 4960
Fragments arranged by size:
4960 922 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2180 2249 2376 2795 2822 2827
Size: 393 422 126 69 127 419 27 5
Cuts at: 2827 2990 3041 3206 3287 3428 4205 4802 5589
Size: 163 51 165 81 141 777 597 787
Cuts at: 5589 5857 1239
Size: 268 1524
Fragments arranged by size:
1524 787 777 597 422 419 393 268
165 163 141 127 126 81 69 51
27 5
EcoO109I rG'GnC_Cy
Cuts at: 2017 2205 2364 3190 2017
Size: 188 159 826 4969
Fragments arranged by size:
4969 826 188 159
EcoRI G'AATT_C
Cuts at: 2777 3451 3829 2777
Size: 674 378 5090
Fragments arranged by size:
5090 674 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 6142
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 5289 1678
Size: 3611 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 2686 2718 4783
Size: 32 135 32 637 32 445 32 2065
Cuts at: 4783 4815 4857 4889 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2065 637 445 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2966 3486 3732 669
Size: 988 281 1028 520 246 3079
Fragments arranged by size:
3079 1028 988 520 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 2953 3467 2953
Size: 514 5628
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2613 3159 5256 5440 1305
Size: 1308 546 2097 184 2007
Fragments arranged by size:
2097 2007 1308 546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 10
Size: 54 1425 343 243 4077
Fragments arranged by size:
4077 1425 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3162 3173 4100 4459 4618 518
Size: 648 1996 11 927 359 159 2042
Fragments arranged by size:
2042 1996 927 648 359 159 11
MspA1I CmG'CkG
Cuts at: 1606 2863 2977 3558 4235 5383 5628 1606
Size: 1257 114 581 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 677 581 245 114
NcoI C'CATG_G
Cuts at: 513 1065 3174 513
Size: 552 2109 3481
Fragments arranged by size:
3481 2109 552
NdeI CA'TA_TG
Cuts at: 387 2348 387
Size: 1961 4181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 2650 3500 5045 1132
Size: 97 1421 850 1545 2229
Fragments arranged by size:
2229 1545 1421 850 97
PasI CC'CwG_GG
Cuts at: 1348 2100 1348
Size: 752 5390
PciI A'CATG_T
Cuts at: 2646 5041 2646
Size: 2395 3747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2071 2632 1108
Size: 963 561 4618
Fragments arranged by size:
4618 963 561
PfoI T'CCnGG_A
Cuts at: 2031 2031
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 3190 2205
Size: 985 5157
PshAI GACnn'nnGTC
Cuts at: 1613 3270 1613
Size: 1657 4485
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 3494 830
Size: 2664 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2863 2977 2863
Size: 114 6028
SacI G_AGCT'C
Cuts at: 2109 3031 3291 2109
Size: 922 260 4960
Fragments arranged by size:
4960 922 260
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SanDI GG'GwC_CC
Cuts at: 2205 2205
Size: 6142
SapI GCTCTTCn'nnn_
Cuts at: 2114 2114
Size: 6142
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2012 3490 4625 5306 5497 826
Size: 219 967 1478 1135 681 191 1471
Fragments arranged by size:
1478 1471 1135 967 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2468 3014 4246 5147 5409 820
Size: 197 926 525 546 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 546 525 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 2069 2132 2177 3436 1517
Size: 552 63 45 1259 4223
Fragments arranged by size:
4223 1259 552 63 45
StyI C'CwwG_G
Cuts at: 513 1065 2070 2420 3174 3581 513
Size: 552 1005 350 754 407 3074
Fragments arranged by size:
3074 1005 754 552 407 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2499 4169 4186 4339 4524 4969 325
Size: 1255 919 1670 17 153 185 445 1498
Fragments arranged by size:
1670 1498 1255 919 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2536 2549 4388
Size: 274 80 33 51 1443 559 13 1839
Cuts at: 4388 6100 96
Size: 1712 138
Fragments arranged by size:
1839 1712 1443 559 274 138 80 51
33 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 6102 6134 2013
Size: 32 4057 32 2021
Fragments arranged by size:
4057 2021 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 3403 2083
Size: 1320 4822
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 2450 2530 4271 2450
Size: 80 1741 4321
Fragments arranged by size:
4321 1741 80
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI
SfiI SfoI SrfI SwaI