Restriction map for
pFN21AB7424
(Circular) (Six-base) MAPSORT of: pFN21AB7424.seq Check: 9489 from: 1 to: 6151
pFN21AB7424 6151 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4214 5598 5866 1632
Size: 2582 1384 268 1917
Fragments arranged by size:
2582 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 2933 3302 3894 5466 2933
Size: 369 592 1572 3618
Fragments arranged by size:
3618 1572 592 369
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2995 3013 3460 3686 3838 5947 1493
Size: 1502 18 447 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 447 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 3054 3404 4705 160
Size: 872 2022 350 1301 1606
Fragments arranged by size:
2022 1606 1301 872 350
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3472 4927 618
Size: 325 217 627 1685 1455 1842
Fragments arranged by size:
1842 1685 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2281 3470 1391
Size: 890 1189 4072
Fragments arranged by size:
4072 1189 890
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2802 928
Size: 971 903 4277
Fragments arranged by size:
4277 971 903
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2493 2527 3430 3464 4342 4376 1903
Size: 34 556 34 903 34 878 34 3678
Fragments arranged by size:
3678 903 878 556 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2772 4043 5253 2772
Size: 1271 1210 3670
Fragments arranged by size:
3670 1271 1210
BclI T'GATC_A
Cuts at: 1548 3031 1548
Size: 1483 4668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 4212 5368 1790
Size: 2422 1156 2573
Fragments arranged by size:
2573 2422 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3882 4840 442
Size: 416 53 83 399 24 2465 958 1753
Fragments arranged by size:
2465 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2136 2138 2136
Size: 2 6149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2293 2958 2986 4811 1239
Size: 1054 665 28 1825 2579
Fragments arranged by size:
2579 1825 1054 665 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3187 4276 5141 5439 3187
Size: 1089 865 298 3899
Fragments arranged by size:
3899 1089 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4814 882
Size: 617 3315 2219
Fragments arranged by size:
3315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 2037 3348 6145 2037
Size: 1311 2797 2043
Fragments arranged by size:
2797 2043 1311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3078 3108 1178
Size: 30 165 30 1675 30 4221
Fragments arranged by size:
4221 1675 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2427 1186
Size: 1241 4910
BseYI C'CCAG_C
Cuts at: 3082 3189 5354 5850 3082
Size: 107 2165 496 3383
Fragments arranged by size:
3383 2165 496 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5578
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4212 4297 5368 4212
Size: 85 1071 4995
Fragments arranged by size:
4995 1071 85
BsmI GAATG_Cn'
Cuts at: 3662 3755 3662
Size: 93 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 3470 4212 4297 5368 1391
Size: 399 491 1189 742 85 1071 2174
Fragments arranged by size:
2174 1189 1071 742 491 399 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3506 844
Size: 2662 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4646 4820 65
Size: 1183 3398 174 1396
Fragments arranged by size:
3398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2897 2933 794
Size: 2103 36 4012
Fragments arranged by size:
4012 2103 36
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstXI CCAn_nnnn'nTGG
Cuts at: 2415 2415
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 3481 3848 4233 4250 5702 5788 5800 6146
Size: 1509 367 385 17 1452 86 12 346
Cuts at: 6146 1972
Size: 1977
Fragments arranged by size:
1977 1509 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2256 2801 513
Size: 552 1191 545 3863
Fragments arranged by size:
3863 1191 552 545
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 3667 4461 4481 3667
Size: 794 20 5337
Fragments arranged by size:
5337 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 2413 3213 3455 2413
Size: 800 242 5109
Fragments arranged by size:
5109 800 242
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2017 5158 809
Size: 507 701 3141 1802
Fragments arranged by size:
3141 1802 701 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116
Size: 54 1425 50 275 18 1768 889 1629
Cuts at: 6116 8
Size: 43
Fragments arranged by size:
1768 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4081 1353
Size: 2728 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3053 4738 5112 5258 387
Size: 1064 138 1464 1685 374 146 1280
Fragments arranged by size:
1685 1464 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2293 2958 2986 4214 4811 5598 5866
Size: 393 661 665 28 1228 597 787 268
Cuts at: 5866 1239
Size: 1524
Fragments arranged by size:
1524 1228 787 665 661 597 393 268
28
EcoRI G'AATT_C
Cuts at: 3013 3460 3838 3013
Size: 447 378 5326
Fragments arranged by size:
5326 447 378
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 5298 1678
Size: 3620 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2931 2963 3043 3075 4792
Size: 32 135 32 1359 32 80 32 1717
Cuts at: 4792 4824 4866 4898 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1717 1359 135 80 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2629 5265 5449 1305
Size: 1324 2636 184 2007
Fragments arranged by size:
2636 2007 1324 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2413 4109 4468 4627 518
Size: 648 1247 1696 359 159 2042
Fragments arranged by size:
2042 1696 1247 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3567 4244 5392 5637 1606
Size: 1961 677 1148 245 2120
Fragments arranged by size:
2120 1961 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2256 2801 513
Size: 552 1191 545 3863
Fragments arranged by size:
3863 1191 552 545
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2641 2988 3509 5054 1132
Size: 97 1412 347 521 1545 2229
Fragments arranged by size:
2229 1545 1412 521 347 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3009 1108
Size: 1901 4250
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3171 3721 3171
Size: 550 5601
PstI C_TGCA'G
Cuts at: 830 2506 3443 3503 830
Size: 1676 937 60 3478
Fragments arranged by size:
3478 1676 937 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 2062 4399 6111 2062
Size: 2337 1712 2102
Fragments arranged by size:
2337 2102 1712
SfcI C'TryA_G
Cuts at: 826 1045 2502 2682 3439 3499 4634 5315 5506
Size: 219 1457 180 757 60 1135 681 191
Cuts at: 5506 826
Size: 1471
Fragments arranged by size:
1471 1457 1135 757 681 219 191 180
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3202 4255 5156 5418 820
Size: 197 926 1259 1053 901 262 1553
Fragments arranged by size:
1553 1259 1053 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 2988 3509 1132
Size: 1856 521 3774
Fragments arranged by size:
3774 1856 521
SspI AAT'ATT
Cuts at: 5 52 3408 4075 5
Size: 47 3356 667 2081
Fragments arranged by size:
3356 2081 667 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2229 2256 2801 3237 3590 513
Size: 552 1164 27 545 436 353 3074
Fragments arranged by size:
3074 1164 552 545 436 353 27
SwaI ATTT'AAAT
Cuts at: 2413 2413
Size: 6151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2355 2584 2590 4178 4195 4348 4533
Size: 1255 775 229 6 1588 17 153 185
Cuts at: 4533 4978 325
Size: 445 1498
Fragments arranged by size:
1588 1498 1255 775 445 229 185 153
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2060 2239 4397
Size: 274 80 33 51 1443 83 179 2158
Cuts at: 4397 6109 96
Size: 1712 138
Fragments arranged by size:
2158 1712 1443 274 179 138 83 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6111 6143 6111
Size: 32 6119
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6151
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2106 2106
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI