Restriction map for
pFN21AB7418
(Circular) (Six-base) MAPSORT of: pFN21AB7418.seq Check: 5485 from: 1 to: 5836
pFN21AB7418 5836 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3899 5283 5551 1632
Size: 2267 1384 268 1917
Fragments arranged by size:
2267 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 5804
AlwNI CAG_nnn'CTG
Cuts at: 2524 2764 3579 5151 2524
Size: 240 815 1572 3209
Fragments arranged by size:
3209 1572 815 240
ApaI G_GGCC'C
Cuts at: 2012 2121 2446 2012
Size: 109 325 5402
Fragments arranged by size:
5402 325 109
ApaLI G'TGCA_C
Cuts at: 2800 3893 5049 2800
Size: 1093 1156 3587
Fragments arranged by size:
3587 1156 1093
ApoI r'AATT_y
Cuts at: 1493 3101 3145 3371 3523 5632 1493
Size: 1608 44 226 152 2109 1697
Fragments arranged by size:
2109 1697 1608 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 3161 1870
Size: 73 177 1041 4545
Fragments arranged by size:
4545 1041 177 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2260 2317 2456 3157 4612
Size: 325 217 627 473 57 139 701 1455
Cuts at: 4612 618
Size: 1842
Fragments arranged by size:
1842 1455 701 627 473 325 217 139
57
BanII G_rGCy'C
Cuts at: 1391 2012 2121 2176 2446 2859 3155 1391
Size: 621 109 55 270 413 296 4072
Fragments arranged by size:
4072 621 413 296 270 109 55
BbeI G_GCGC'C
Cuts at: 2264 2264
Size: 5836
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2791 2825 4027 4061 1903
Size: 34 854 34 1202 34 3678
Fragments arranged by size:
3678 1202 854 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BfrBI ATG'CAT
Cuts at: 2582 2582
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2101 2258 2727
Size: 107 122 71 631 605 429 157 469
Cuts at: 2727 4447 136
Size: 1720 1525
Fragments arranged by size:
1720 1525 631 605 469 429 157 122
107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 2933 3253 2933
Size: 320 5516
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2121 2446 2740 2804 3897 5053 1790
Size: 222 109 325 294 64 1093 1156 2573
Fragments arranged by size:
2573 1156 1093 325 294 222 109 64
BmgBI CAC'GTC
Cuts at: 1318 2194 1318
Size: 876 4960
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2365 3567 4525
Size: 416 53 83 399 24 948 1202 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1202 958 948 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 3062 4496 1239
Size: 1007 816 1434 2579
Fragments arranged by size:
2579 1434 1007 816
Bpu10I CC'TnA_GC
Cuts at: 2528 2528
Size: 5836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2897 3961 4826 5124 2897
Size: 1064 865 298 3609
Fragments arranged by size:
3609 1064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2966 4499 882
Size: 617 1467 1533 2219
Fragments arranged by size:
2219 1533 1467 617
BsaAI yAC'GTr
Cuts at: 493 1204 2350 3627 493
Size: 711 1146 1277 2702
Fragments arranged by size:
2702 1277 1146 711
BsaBI GATnn'nnATC
Cuts at: 2294 5830 2294
Size: 3536 2300
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2261 2834 3643
Size: 53 83 186 936 699 29 573 809
Cuts at: 3643 4025 4660 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 809 699 635 573 382 186
83 53 29
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2853 2883 3000 3030 3110
Size: 30 165 30 1450 30 117 30 80
Cuts at: 3110 3140 1178
Size: 30 3874
Fragments arranged by size:
3874 1450 165 117 80 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5836
BseYI C'CCAG_C
Cuts at: 2104 2269 2517 2823 5039 5535 2104
Size: 165 248 306 2216 496 2405
Fragments arranged by size:
2405 2216 496 306 248 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2154 2278 3286 4047 5075 1540
Size: 275 186 153 124 1008 761 1028 2301
Fragments arranged by size:
2301 1028 1008 761 275 186 153 124
BsiHKAI G_wGCw'C
Cuts at: 2804 2859 3897 3982 5053 2804
Size: 55 1038 85 1071 3587
Fragments arranged by size:
3587 1071 1038 85 55
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
BsmBI CGTCTCn'nnnn_
Cuts at: 2070 2070
Size: 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2121 2176 2446 2740 2804 2859
Size: 399 222 109 55 270 294 64 55
Cuts at: 2859 3155 3897 3982 5053 1391
Size: 296 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 296 294 270 222
109 85 64 55 55
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3095 3191 844
Size: 2251 96 3489
Fragments arranged by size:
3489 2251 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2018 3723 1394
Size: 429 195 1705 3507
Fragments arranged by size:
3507 1705 429 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 2582 4331 4505 65
Size: 1183 1334 1749 174 1396
Fragments arranged by size:
1749 1396 1334 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2146 2928 4480 4667 1700
Size: 110 336 782 1552 187 2869
Fragments arranged by size:
2869 1552 782 336 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 2810 3038 3896 4908 2810
Size: 228 858 1012 3738
Fragments arranged by size:
3738 1012 858 228
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2575 2782 2422
Size: 153 207 5476
Fragments arranged by size:
5476 207 153
BstYI r'GATC_y
Cuts at: 1972 2959 3166 3533 3918 3935 5387 5473 5485
Size: 987 207 367 385 17 1452 86 12
Cuts at: 5485 5831 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 987 385 367 346 207 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 2604 513
Size: 552 939 123 477 3745
Fragments arranged by size:
3745 939 552 477 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 2401 3352 4146 4166 2346
Size: 55 951 794 20 4016
Fragments arranged by size:
4016 951 794 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DraIII CAC_nnn'GTG
Cuts at: 2494 2494
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2219 4843 809
Size: 507 903 2624 1802
Fragments arranged by size:
2624 1802 903 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2787 3283
Size: 54 1425 50 275 18 321 636 496
Cuts at: 3283 4172 5801 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 636 496 321 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2151 3283 1537
Size: 275 339 1132 4090
Fragments arranged by size:
4090 1132 339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2453 3766 1353
Size: 1100 1313 3423
Fragments arranged by size:
3423 1313 1100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2040 2147 2184 4423 4797 4943
Size: 1064 138 451 107 37 2239 374 146
Cuts at: 4943 387
Size: 1280
Fragments arranged by size:
2239 1280 1064 451 374 146 138 107
37
EcoICRI GAG'CTC
Cuts at: 2857 2857
Size: 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 3062 3899 4496 5283 5551 1239
Size: 393 614 816 837 597 787 268 1524
Fragments arranged by size:
1524 837 816 787 614 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 2684
Size: 5836
EcoO109I rG'GnC_Cy
Cuts at: 2009 2117 2419 2443 2595 2950 2009
Size: 108 302 24 152 355 4895
Fragments arranged by size:
4895 355 302 152 108 24
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 5836
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2264 2887 2926 4983 1678
Size: 586 623 39 2057 2531
Fragments arranged by size:
2531 2057 623 586 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 4477 4509 4551
Size: 32 135 32 1046 32 1827 32 42
Cuts at: 4551 4583 1373
Size: 32 2626
Fragments arranged by size:
2626 1827 1046 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 3180 3426 669
Size: 988 281 720 522 246 3079
Fragments arranged by size:
3079 988 720 522 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KasI G'GCGC_C
Cuts at: 2260 2260
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 4950 5134 1305
Size: 1251 2394 184 2007
Fragments arranged by size:
2394 2007 1251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2789 10
Size: 54 1425 343 957 3057
Fragments arranged by size:
3057 1425 957 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3794 4153 4312 518
Size: 648 2628 359 159 2042
Fragments arranged by size:
2628 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2098 2129 2622 2764 2827 2851 3252
Size: 465 27 31 493 142 63 24 401
Cuts at: 3252 3929 5077 5322 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 493 465 401 245 142
63 31 27 24
NaeI GCC'GGC
Cuts at: 2148 2930 2148
Size: 782 5054
NarI GG'CG_CC
Cuts at: 2261 2261
Size: 5836
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NgoMIV G'CCGG_C
Cuts at: 2146 2928 2146
Size: 782 5054
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NsiI A_TGCA'T
Cuts at: 2584 2584
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2494 1108
Size: 1386 4450
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 3905 3937 5418 5450 3110
Size: 32 763 32 1481 32 3496
Fragments arranged by size:
3496 1481 763 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2595 2595
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PspOMI G'GGCC_C
Cuts at: 2008 2117 2442 2008
Size: 109 325 5402
Fragments arranged by size:
5402 325 109
PstI C_TGCA'G
Cuts at: 830 2592 2760 3188 830
Size: 1762 168 428 3478
Fragments arranged by size:
3478 1762 428 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2764 2764
Size: 5836
SacI G_AGCT'C
Cuts at: 2859 2859
Size: 5836
SacII CC_GC'GG
Cuts at: 2130 2130
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2588 2756 3184 4319 5000 5191 826
Size: 219 1543 168 428 1135 681 191 1471
Fragments arranged by size:
1543 1471 1135 681 428 219 191 168
SfoI GGC'GCC
Cuts at: 2262 2262
Size: 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 2122 3163 1872
Size: 250 1041 4545
Fragments arranged by size:
4545 1041 250
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2912 3940 4841 5103 820
Size: 197 926 969 1028 901 262 1553
Fragments arranged by size:
1553 1028 969 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 2408 2808 1517
Size: 891 400 4545
Fragments arranged by size:
4545 891 400
StyI C'CwwG_G
Cuts at: 513 1065 2004 2415 2685 2722 3275 513
Size: 552 939 411 270 37 553 3074
Fragments arranged by size:
3074 939 553 552 411 270 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2444 3863 3880 4033 4218 4663 325
Size: 1255 864 1419 17 153 185 445 1498
Fragments arranged by size:
1498 1419 1255 864 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2659 2691 2853 2885 5796 5828 2659
Size: 32 162 32 2911 32 2667
Fragments arranged by size:
2911 2667 162 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2714 1316
Size: 1398 4438
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2328 2782 2328
Size: 454 5382
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 2120 3161 1870
Size: 250 1041 4545
Fragments arranged by size:
4545 1041 250
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BplI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI NruI PacI PmlI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI
SwaI