Restriction map for
pFN21AB7417
(Circular) (Six-base) MAPSORT of: pFN21AB7417.seq Check: 9047 from: 1 to: 5800
pFN21AB7417 5800 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2477 2477
Size: 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 3863 5247 5515 1632
Size: 846 1385 1384 268 1917
Fragments arranged by size:
1917 1385 1384 846 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2215 2309 2592 3077 1166
Size: 1049 94 283 485 3889
Fragments arranged by size:
3889 1049 485 283 94
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3025 3125 1870
Size: 73 1082 100 4545
Fragments arranged by size:
4545 1082 100 73
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2522 3121 4576 618
Size: 325 217 627 735 599 1455 1842
Fragments arranged by size:
1842 1455 735 627 599 325 217
BanII G_rGCy'C
Cuts at: 1391 3119 1391
Size: 1728 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2677 928
Size: 971 778 4051
Fragments arranged by size:
4051 971 778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2025 3692 4902 2025
Size: 1667 1210 2923
Fragments arranged by size:
2923 1667 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2427 3861 5017 1790
Size: 637 1434 1156 2573
Fragments arranged by size:
2573 1434 1156 637
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 3531 4489
Size: 416 53 83 399 24 1169 945 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1169 958 945 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 2978
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2635 3002 4460 1239
Size: 1184 212 367 1458 2579
Fragments arranged by size:
2579 1458 1184 367 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2346 3925 4790 5088 2346
Size: 1579 865 298 3058
Fragments arranged by size:
3058 1579 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2742 4463 882
Size: 617 1243 1721 2219
Fragments arranged by size:
2219 1721 1243 617
BsaAI yAC'GTr
Cuts at: 493 1204 2370 3061 3591 493
Size: 711 1166 691 530 2702
Fragments arranged by size:
2702 1166 711 691 530
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2660 2690 3038 3068 1178
Size: 30 165 30 1257 30 348 30 3910
Fragments arranged by size:
3910 1257 348 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 3065 5003 5499 3065
Size: 1938 496 3366
Fragments arranged by size:
3366 1938 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3007 3101 1163
Size: 573 1271 94 3862
Fragments arranged by size:
3862 1271 573 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3861 3946 5017 3861
Size: 85 1071 4644
Fragments arranged by size:
4644 1071 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 3119 3861 3946 5017 1391
Size: 399 637 692 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 692 637 399 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2477 3155 844
Size: 1633 678 3489
Fragments arranged by size:
3489 1633 678
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 4295 4469 65
Size: 1183 1759 1288 174 1396
Fragments arranged by size:
1759 1396 1288 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3018 794
Size: 2224 3576
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 3065 3065
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2286 3130 3497 3882 3899 5351 5437 5449
Size: 314 844 367 385 17 1452 86 12
Cuts at: 5449 5795 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 844 385 367 346 314 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2453 2453
Size: 5800
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3316 4110 4130 2995
Size: 321 794 20 4665
Fragments arranged by size:
4665 794 321 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3247 4136 5765
Size: 54 1425 50 275 18 1417 889 1629
Cuts at: 5765 8
Size: 43
Fragments arranged by size:
1629 1425 1417 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3730 1353
Size: 2377 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2478 2635 3002 3863 4460 5247
Size: 393 791 55 157 367 861 597 787
Cuts at: 5247 5515 1239
Size: 268 1524
Fragments arranged by size:
1524 861 791 787 597 393 367 268
157 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2402
Size: 5800
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 3088 4947 1678
Size: 1410 1859 2531
Fragments arranged by size:
2531 1859 1410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2989 3021 3038 3070 4441
Size: 32 135 32 1417 32 17 32 1371
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 1371 135 42 32 32 32
32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2082 3144 3390 669
Size: 988 281 144 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2898 4914 5098 1305
Size: 1593 2016 184 2007
Fragments arranged by size:
2016 2007 1593 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2215 2309 2592 3077 3758 4117 4276
Size: 648 1049 94 283 485 681 359 159
Cuts at: 4276 518
Size: 2042
Fragments arranged by size:
2042 1049 681 648 485 359 283 159
94
MspA1I CmG'CkG
Cuts at: 1606 2389 3216 3893 5041 5286 1606
Size: 783 827 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 783 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 2784 387
Size: 2397 3403
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 1108
Size: 1629 4171
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2378 3152 830
Size: 1548 774 3478
Fragments arranged by size:
3478 1548 774
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2389 2389
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SexAI A'CCwGG_T
Cuts at: 2529 2529
Size: 5800
SfcI C'TryA_G
Cuts at: 826 1045 2374 3148 4283 4964 5155 826
Size: 219 1329 774 1135 681 191 1471
Fragments arranged by size:
1471 1329 1135 774 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3027 3127 1872
Size: 1155 100 4545
Fragments arranged by size:
4545 1155 100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2361 3904 4805 5067 820
Size: 197 926 418 1543 901 262 1553
Fragments arranged by size:
1553 1543 926 901 418 262 197
SnaBI TAC'GTA
Cuts at: 493 2370 3591 493
Size: 1877 1221 2702
Fragments arranged by size:
2702 1877 1221
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 2195 2830 3724 5
Size: 47 2143 635 894 2081
Fragments arranged by size:
2143 2081 894 635 47
StuI AGG'CCT
Cuts at: 1517 2776 2798 1517
Size: 1259 22 4519
Fragments arranged by size:
4519 1259 22
StyI C'CwwG_G
Cuts at: 513 1065 2004 2173 2629 3239 513
Size: 552 939 169 456 610 3074
Fragments arranged by size:
3074 939 610 552 456 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 2321 3827 3844 3997 4182 4627
Size: 1255 728 13 1506 17 153 185 445
Cuts at: 4627 325
Size: 1498
Fragments arranged by size:
1506 1498 1255 728 445 185 153 17
13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 2733 4046
Size: 274 80 33 51 1443 489 267 1313
Cuts at: 4046 5758 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1313 489 274 267 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2029 2061 3061 3093 5760 5792 2029
Size: 32 1000 32 2667 32 2037
Fragments arranged by size:
2667 2037 1000 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2479 2479
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3025 3125 1870
Size: 1155 100 4545
Fragments arranged by size:
4545 1155 100
XmnI GAAnn'nnTTC
Cuts at: 2183 2830 3929 2183
Size: 647 1099 4054
Fragments arranged by size:
4054 1099 647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI