Restriction map for
pFN21AB7414
(Circular) (Six-base) MAPSORT of: pFN21AB7414.seq Check: 4348 from: 1 to: 5971
pFN21AB7414 5971 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2323 2686 2854 3190 4034 5418 5686 1632
Size: 691 363 168 336 844 1384 268 1917
Fragments arranged by size:
1917 1384 844 691 363 336 268 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 2661 1166
Size: 1495 4476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 3714 5286 3714
Size: 1572 4399
ApaI G_GGCC'C
Cuts at: 2107 2107
Size: 5971
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 3072 3280 3506 3658 5767 1493
Size: 1579 208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1579 226 208 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 1870
Size: 73 1353 4545
Fragments arranged by size:
4545 1353 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2475 3292 4747 618
Size: 325 217 627 235 453 817 1455 1842
Fragments arranged by size:
1842 1455 817 627 453 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2107 3290 1391
Size: 716 1183 4072
Fragments arranged by size:
4072 1183 716
BbeI G_GCGC'C
Cuts at: 2026 2026
Size: 5971
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2878 3260 928
Size: 971 979 382 3639
Fragments arranged by size:
3639 979 971 382
BbvCI CC'TCA_GC
Cuts at: 2467 2467
Size: 5971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2107 4032 5188 1790
Size: 317 1925 1156 2573
Fragments arranged by size:
2573 1925 1156 317
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2922 3006 3090
Size: 416 53 83 399 24 1505 84 84
Cuts at: 3090 3702 4660 442
Size: 612 958 1753
Fragments arranged by size:
1753 1505 958 612 416 399 84 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2213 2850 2876 4631 1239
Size: 908 66 637 26 1755 2579
Fragments arranged by size:
2579 1755 908 637 66 26
Bpu10I CC'TnA_GC
Cuts at: 2467 2467
Size: 5971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2950 4096 4961 5259 2950
Size: 1146 865 298 3662
Fragments arranged by size:
3662 1146 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2188 4634 882
Size: 617 689 2446 2219
Fragments arranged by size:
2446 2219 689 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2023 3778 4160 4795
Size: 53 83 186 936 490 1755 382 635
Cuts at: 4795 275
Size: 1451
Fragments arranged by size:
1755 1451 936 635 490 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5746
Fragments arranged by size:
5746 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2461 1186
Size: 1275 4696
BseYI C'CCAG_C
Cuts at: 2187 2376 5174 5670 2187
Size: 189 2798 496 2488
Fragments arranged by size:
2798 2488 496 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 1163
Size: 573 381 5017
Fragments arranged by size:
5017 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4032 4117 5188 4032
Size: 85 1071 4815
Fragments arranged by size:
4815 1071 85
BsmI GAATG_Cn'
Cuts at: 2156 2684 2994 3119 3482 3575 2156
Size: 528 310 125 363 93 4552
Fragments arranged by size:
4552 528 363 310 125 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 3290 4032 4117 5188 1391
Size: 399 317 1183 742 85 1071 2174
Fragments arranged by size:
2174 1183 1071 742 399 317 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4466 4640 65
Size: 1183 3218 174 1396
Fragments arranged by size:
3218 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 2109 96
Size: 2013 3958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966
Size: 1329 367 385 17 1452 86 12 346
Cuts at: 5966 1972
Size: 1977
Fragments arranged by size:
1977 1452 1329 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2210 513
Size: 552 1145 4274
Fragments arranged by size:
4274 1145 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2671 2706 529
Size: 35 2107 35 3794
Fragments arranged by size:
3794 2107 35 35
DraI TTT'AAA
Cuts at: 2761 3275 2761
Size: 514 5457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3901 1353
Size: 2548 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2213 2323 2686 2850 2854 2876
Size: 393 515 66 110 363 164 4 22
Cuts at: 2876 3190 4034 4631 5418 5686 1239
Size: 314 844 597 787 268 1524
Fragments arranged by size:
1524 844 787 597 515 393 363 314
268 164 110 66 22 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2470 2470
Size: 5971
EcoO109I rG'GnC_Cy
Cuts at: 2103 2104 2215 2356 2103
Size: 1 111 141 5718
Fragments arranged by size:
5718 141 111 1
EcoRI G'AATT_C
Cuts at: 3072 3280 3658 3072
Size: 208 378 5385
Fragments arranged by size:
5385 378 208
EcoRV GAT'ATC
Cuts at: 2586 2586
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2026 5118 1678
Size: 348 3092 2531
Fragments arranged by size:
3092 2531 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2797 2829 3049
Size: 32 135 32 731 32 462 32 220
Cuts at: 3049 3081 4612 4644 4686 4718 1373
Size: 32 1531 32 42 32 2626
Fragments arranged by size:
2626 1531 731 462 220 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KasI G'GCGC_C
Cuts at: 2022 2022
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2924 3069 5085 5269 1305
Size: 1619 145 2016 184 2007
Fragments arranged by size:
2016 2007 1619 184 145
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 2643 2661 2761 3929 4288 4447
Size: 648 1458 19 18 100 1168 359 159
Cuts at: 4447 518
Size: 2042
Fragments arranged by size:
2042 1458 1168 648 359 159 100 19
18
MspA1I CmG'CkG
Cuts at: 1606 2191 3210 3387 4064 5212 5457 1606
Size: 585 1019 177 677 1148 245 2120
Fragments arranged by size:
2120 1148 1019 677 585 245 177
NarI GG'CG_CC
Cuts at: 2023 2023
Size: 5971
NcoI C'CATG_G
Cuts at: 513 1065 2210 513
Size: 552 1145 4274
Fragments arranged by size:
4274 1145 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2226 1348
Size: 878 5093
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3260 1613
Size: 1647 4324
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PspOMI G'GGCC_C
Cuts at: 2103 2103
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3323 830
Size: 2493 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2191 3210 2191
Size: 1019 4952
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 3319 4454 5135 5326 826
Size: 219 2274 1135 681 191 1471
Fragments arranged by size:
2274 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2024 2024
Size: 5971
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2965 4075 4976 5238 820
Size: 197 926 1022 1110 901 262 1553
Fragments arranged by size:
1553 1110 1022 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 2533 3895 5
Size: 47 2481 1362 2081
Fragments arranged by size:
2481 2081 1362 47
StuI AGG'CCT
Cuts at: 1517 2486 2867 1517
Size: 969 381 4621
Fragments arranged by size:
4621 969 381
StyI C'CwwG_G
Cuts at: 513 1065 2210 3410 513
Size: 552 1145 1200 3074
Fragments arranged by size:
3074 1200 1145 552
SwaI ATTT'AAAT
Cuts at: 2761 2761
Size: 5971
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 3998 4015 4168 4353 4798 325
Size: 1255 501 1917 17 153 185 445 1498
Fragments arranged by size:
1917 1498 1255 501 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2109 4217
Size: 274 80 33 51 1443 81 51 2108
Cuts at: 4217 5929 96
Size: 1712 138
Fragments arranged by size:
2108 1712 1443 274 138 81 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 2437 1316
Size: 1121 4850
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2418 2176
Size: 242 5729
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 2951 3119 4100 2951
Size: 168 981 4822
Fragments arranged by size:
4822 981 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI