Restriction map for
pFN21AB7412
(Circular) (Six-base) MAPSORT of: pFN21AB7412.seq Check: 2732 from: 1 to: 5722
pFN21AB7412 5722 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2850 3532 278
Size: 53 83 186 936 1314 682 2468
Fragments arranged by size:
2468 1314 936 682 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2382 2883 3000 3785 5169 5437 1632
Size: 750 501 117 785 1384 268 1917
Fragments arranged by size:
1917 1384 785 750 501 268 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 2034 1166
Size: 868 4854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 3465 5037 3465
Size: 1572 4150
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2128 2697 2731 2884 2947 3031 3257 3409
Size: 635 569 34 153 63 84 226 152
Cuts at: 3409 5518 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 635 569 226 153 152 84
63 34
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 3047 1870
Size: 73 1104 4545
Fragments arranged by size:
4545 1104 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2749 2782 2749
Size: 33 5689
BamHI G'GATC_C
Cuts at: 2791 3052 3419 2791
Size: 261 367 5094
Fragments arranged by size:
5094 367 261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2293 2475 2929 2960
Size: 325 217 627 360 146 182 454 31
Cuts at: 2960 3043 4498 618
Size: 83 1455 1842
Fragments arranged by size:
1842 1455 627 454 360 325 217 182
146 83 31
BanII G_rGCy'C
Cuts at: 1391 2368 3041 1391
Size: 977 673 4072
Fragments arranged by size:
4072 977 673
BbeI G_GCGC'C
Cuts at: 2151 2297 2479 2151
Size: 146 182 5394
Fragments arranged by size:
5394 182 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2774 928
Size: 971 875 3876
Fragments arranged by size:
3876 971 875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3913 3947 1903
Size: 34 50 34 1892 34 3678
Fragments arranged by size:
3678 1892 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2777 3614 4824 2777
Size: 837 1210 3675
Fragments arranged by size:
3675 1210 837
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4333 136
Size: 107 122 71 631 605 2661 1525
Fragments arranged by size:
2661 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2264 2932 3783 4939 1790
Size: 474 668 851 1156 2573
Fragments arranged by size:
2573 1156 851 668 474
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 2237 3021
Size: 416 53 83 399 24 636 184 784
Cuts at: 3021 3453 4411 442
Size: 432 958 1753
Fragments arranged by size:
1753 958 784 636 432 416 399 184
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2241 1056
Size: 1185 4537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2619 2619
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 2874 4382 1239
Size: 1388 247 1508 2579
Fragments arranged by size:
2579 1508 1388 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2848 3847 4712 5010 2848
Size: 999 865 298 3560
Fragments arranged by size:
3560 999 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 5716 5716
Size: 5722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2294 2476 2847
Size: 53 83 186 936 615 146 182 371
Cuts at: 2847 3529 3911 4546 275
Size: 682 382 635 1451
Fragments arranged by size:
1451 936 682 635 615 382 371 186
182 146 83 53
BsaWI w'CCGG_w
Cuts at: 4155 4553 4827 4974 5385 4155
Size: 398 274 147 411 4492
Fragments arranged by size:
4492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2600 2630 1178
Size: 30 165 30 1197 30 4270
Fragments arranged by size:
4270 1197 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 1186
Size: 874 4848
BseYI C'CCAG_C
Cuts at: 2012 4925 5421 2012
Size: 2913 496 2313
Fragments arranged by size:
2913 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2196 3172 3933 4961 1540
Size: 275 186 21 174 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186 174 21
BsiHKAI G_wGCw'C
Cuts at: 2328 2435 3783 3868 4939 2328
Size: 107 1348 85 1071 3111
Fragments arranged by size:
3111 1348 1071 107 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2328 2368 2435 2932 3041 3783
Size: 399 474 64 40 67 497 109 742
Cuts at: 3783 3868 4939 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 497 474 399 109 85
67 64 40
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 4366 4553 1700
Size: 110 223 2333 187 2869
Fragments arranged by size:
2869 2333 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 2214 1925
Size: 289 5433
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstXI CCAn_nnnn'nTGG
Cuts at: 2671 2671
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2791 3052 3419 3804 3821 5273 5359 5371
Size: 819 261 367 385 17 1452 86 12
Cuts at: 5371 5717 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 819 385 367 346 261 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 4027
Fragments arranged by size:
4027 1143 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 2490 2951 3238 4032 4052 2490
Size: 461 287 794 20 4160
Fragments arranged by size:
4160 794 461 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 3169 4058
Size: 54 1425 50 275 18 372 967 889
Cuts at: 4058 5687 8
Size: 1629 43
Fragments arranged by size:
1629 1425 967 889 372 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2502 2566 3652 1353
Size: 1149 64 1086 3423
Fragments arranged by size:
3423 1149 1086 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2743 4309 4683 4829 387
Size: 1064 138 1154 1566 374 146 1280
Fragments arranged by size:
1566 1280 1154 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2382 2627 2874 2883 3000 3785 4382
Size: 393 750 245 247 9 117 785 597
Cuts at: 4382 5169 5437 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 785 750 597 393 268 247
245 117 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2531
Size: 5722
EcoO109I rG'GnC_Cy
Cuts at: 2466 2466
Size: 5722
EcoRI G'AATT_C
Cuts at: 2731 2884 3031 3409 2731
Size: 153 147 378 5044
Fragments arranged by size:
5044 378 153 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2798 2798
Size: 5722
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 2151 2297 2405 2479 4869 1678
Size: 473 146 108 74 2390 2531
Fragments arranged by size:
2531 2390 473 146 108 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2783 2815 4363 4395 4437
Size: 32 135 32 1211 32 1548 32 42
Cuts at: 4437 4469 1373
Size: 32 2626
Fragments arranged by size:
2626 1548 1211 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3312 669
Size: 988 281 1128 246 3079
Fragments arranged by size:
3079 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KasI G'GCGC_C
Cuts at: 2147 2293 2475 2147
Size: 146 182 5394
Fragments arranged by size:
5394 182 146
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2781 4836 5020 1305
Size: 1476 2055 184 2007
Fragments arranged by size:
2055 2007 1476 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2204 10
Size: 54 1425 343 372 3528
Fragments arranged by size:
3528 1425 372 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2034 3680 4039 4198 518
Size: 648 868 1646 359 159 2042
Fragments arranged by size:
2042 1646 868 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2210 3138 3815 4963 5208 1606
Size: 478 126 928 677 1148 245 2120
Fragments arranged by size:
2120 1148 928 677 478 245 126
NarI GG'CG_CC
Cuts at: 2148 2294 2476 2148
Size: 146 182 5394
Fragments arranged by size:
5394 182 146
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 2237 1052
Size: 1185 4537
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 2518 3080 4625 1132
Size: 97 1289 562 1545 2229
Fragments arranged by size:
2229 1545 1289 562 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5722
PfoI T'CCnGG_A
Cuts at: 2913 2913
Size: 5722
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2466 2466
Size: 5722
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 2169 3074 830
Size: 1339 905 3478
Fragments arranged by size:
3478 1339 905
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
SacII CC_GC'GG
Cuts at: 2211 2211
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SapI GCTCTTCn'nnn_
Cuts at: 2566 2566
Size: 5722
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2165 3070 4205 4886 5077 826
Size: 219 1120 905 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 905 681 219 191
SfoI GGC'GCC
Cuts at: 2149 2295 2477 2149
Size: 146 182 5394
Fragments arranged by size:
5394 182 146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 2033 1700
Size: 333 5389
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2863 3826 4727 4989 820
Size: 197 926 920 963 901 262 1553
Fragments arranged by size:
1553 963 926 920 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 2518 3080 1132
Size: 1386 562 3774
Fragments arranged by size:
3774 1386 562
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 3161 513
Size: 552 2096 3074
Fragments arranged by size:
3074 2096 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2391 2664 3749 3766 3919 4104 4549
Size: 1255 811 273 1085 17 153 185 445
Cuts at: 4549 325
Size: 1498
Fragments arranged by size:
1498 1255 1085 811 445 273 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3004 3968 5680
Size: 274 80 33 51 1443 1027 964 1712
Cuts at: 5680 96
Size: 138
Fragments arranged by size:
1712 1443 1027 964 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 5682 5714 2482
Size: 32 3168 32 2490
Fragments arranged by size:
3168 2490 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 3851
Size: 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2848 3530 276
Size: 53 83 186 936 1314 682 2468
Fragments arranged by size:
2468 1314 936 682 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI
SfiI SrfI SwaI XcmI